LOCUS       BBa_K136055             1086 bp    DNA     linear       26-Dec-2024
DEFINITION  flhB promoter + WT RBS (K136008) - mRFP tripart + LVA (I732078)
ACCESSION   BBa_K136055
VERSION     BBa_K136055.1  
FEATURES             Location/Qualifiers
     misc_feature    79..94
                     /label=flhDC binding site
     misc_feature    106..121
                     /label=flhDC binding site
     misc_feature    153..153
                     /label=transcription initiation site
     misc_feature    178..180
                     /label=GTG
     RBS             166..173
                     /label=natural flhB RBS
     promoter        1..203
                     /label=BBa_K136008
     misc_feature    216..219
                     /label=conserved
     RBS             212..223
                     /label=BBa_B0034
     CDS             230..904
                     /label=mrfp1
     misc_feature    944..949
                     /label=Stop
     misc_feature    905..943
                     /label=LVA
     misc_feature    230..232
                     /label=Start
     misc_feature    230..949
                     /label=BBa_J04051
     stem_loop       969..1012
                     /label=stem_loop
     misc_feature    958..1037
                     /label=BBa_B0010
     terminator      958..1037
                     /label=BBa_B0010
     stem_loop       1053..1072
                     /label=T7 TE
     misc_feature    1079..1079
                     /label=stop
     polyA_site      1073..1086
                     /label=polya
     misc_feature    1046..1086
                     /label=BBa_B0012
     terminator      1046..1086
                     /label=BBa_B0012
ORIGIN
        1 ccacgtcata tcaggcggtc tgataaggcg atgacgccgc atccgacaaa cgcacactat
       61 gcttgatgtg tggcagcaaa agccctaaat cccgcctgtt ttgcccctta ctcaaaccat
      121 tgaacgcttt gcgctctggc atcattcacg cttaatactc tttccaggat tggcgacgtg
      181 tctgacgaga gcgacgacaa aactactaga gaaagaggag aaatactaga tggcttcctc
      241 cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg
      301 tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc
      361 taaactgaaa gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca
      421 gttccagtac ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa
      481 actgtccttc ccggaaggtt tcaaatggga acgtgttatg aacttcgaag acggtggtgt
      541 tgttaccgtt acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact
      601 gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga
      661 agcttccacc gaacgtatgt acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg
      721 tctgaaactg aaagacggtg gtcactacga cgctgaagtt aaaaccacct acatggctaa
      781 aaaaccggtt cagctgccgg gtgcttacaa aaccgacatc aaactggaca tcacctccca
      841 caacgaagac tacaccatcg ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg
      901 tgctaggcct gctgcaaacg acgaaaacta cgctttagta gcttaataat actagagcca
      961 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
     1021 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
     1081 tttata
//