##gff-version 3
##sequence-region BBa_K136056 1 1610
BBa_K136056	.	engineered_region	694	1610	.	+	0	ID=BBa_E0422;Name=BBa_E0422
BBa_K136056	.	terminator	1570	1610	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0422
BBa_K136056	.	stem_loop	1577	1596	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K136056	.	stop_codon	1603	1603	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K136056	.	polyA_site	1597	1610	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K136056	.	engineered_region	1570	1610	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K136056	.	terminator	1482	1561	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0422
BBa_K136056	.	stem_loop	1493	1536	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K136056	.	engineered_region	1482	1561	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K136056	.	ribosome_entry_site	694	705	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0422
BBa_K136056	.	conserved_region	698	701	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K136056	.	engineered_region	712	1473	.	+	0	ID=BBa_E0022;Name=BBa_E0022;Parent=BBa_E0422
BBa_K136056	.	engineered_region	712	1473	.	+	0	ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022
BBa_K136056	.	tag	1430	1467	.	+	0	ID=annotation2153;Name=SsrA;Parent=BBa_E0022
BBa_K136056	.	CDS	712	1473	.	+	0	ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022
BBa_K136056	.	stop_codon	1468	1473	.	+	0	ID=annotation2154;Name=2;Parent=BBa_E0022
BBa_K136056	.	point_mutation	780	780	.	+	0	ID=annotation2155;Name=A;Parent=BBa_E0022
BBa_K136056	.	engineered_region	1	685	.	+	0	ID=BBa_S03154;Name=BBa_S03154
BBa_K136056	.	CDS	19	660	.	+	0	ID=BBa_C0078;Name=BBa_C0078;Parent=BBa_S03154
BBa_K136056	.	engineered_tag	661	685	.	+	0	ID=annotation2214003;Name=Help:Barcodes;Parent=BBa_C0078
BBa_K136056	.	CDS	19	621	.	+	0	ID=annotation305970;Name=LasI;Parent=BBa_C0078
BBa_K136056	.	tag	622	654	.	+	0	ID=annotation306608;Name=LVA;Parent=BBa_C0078
BBa_K136056	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03154
BBa_K136056	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K136056
aaagaggagaaatactagatgatcgttcagatcggtcgtcgtgaagagttcgacaaaaaactgctgggtgaaatgcaca
aactgcgtgctcaggttttcaaagaacgtaaaggttgggacgtttccgttatcgacgaaatggaaatcgacggttacga
cgctctgtccccgtactacatgctgatccaggaagacaccccggaagctcaggttttcggttgctggcgtatcttcgac
accaccggtccgtacatgctgaaaaacaccttcccggaactgctgcacggtaaagaagctccgtgctccccgcacatct
gggaactgtcccgtttcgctatcaactccggtcagaaaggttccctgggtttctccgactgcaccctggaagctatgcg
tgctctggctcgttactccttgcagaacgacatccagaccctggttaccgttaccaccgttggtgttgaaaaaatgatg
atccgtgctggtctggacgtttcccgtttcggtccgcacctgaaaatcggtatcgaacgtgctgttgctctgcgtatcg
aactgaacgctaaaacccagatcgctctgtacggtggtgttctggttgaacagcgtctggctgtttccgctgctaacga
cgaaaactacgctctggttgcttaataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactag
atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccaca
agttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaa
gctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatg
aagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggca
actacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaa
ggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcag
aagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagc
agaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaaga
ccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctg
tacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacga
aaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggct
caccttcgggtgggcctttctgcgtttata