LOCUS BBa_K136057 1571 bp DNA linear 26-Dec-2024 DEFINITION RBS - lasI - GFP tTripart ACCESSION BBa_K136057 VERSION BBa_K136057.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 CDS 19..621 /label=LasI misc_feature 622..654 /label=LVA misc_feature 661..685 /label=Help:Barcodes CDS 19..660 /label=BBa_C0078 misc_feature 1..685 /label=BBa_S03154 RBS 694..708 /label=RBS-1\Strong RBS 701..705 /label=RBS misc_feature 694..708 /label=BBa_B0030 RBS 694..708 /label=BBa_B0030 CDS 715..1434 /label=GFP protein CDS 715..1434 /label=BBa_E0040 stem_loop 1454..1497 /label=stem_loop misc_feature 1443..1522 /label=BBa_B0010 terminator 1443..1522 /label=BBa_B0010 stem_loop 1538..1557 /label=T7 TE misc_feature 1564..1564 /label=stop polyA_site 1558..1571 /label=polya misc_feature 1531..1571 /label=BBa_B0012 terminator 1531..1571 /label=BBa_B0012 misc_feature 694..1571 /label=BBa_E0840 ORIGIN 1 aaagaggaga aatactagat gatcgttcag atcggtcgtc gtgaagagtt cgacaaaaaa 61 ctgctgggtg aaatgcacaa actgcgtgct caggttttca aagaacgtaa aggttgggac 121 gtttccgtta tcgacgaaat ggaaatcgac ggttacgacg ctctgtcccc gtactacatg 181 ctgatccagg aagacacccc ggaagctcag gttttcggtt gctggcgtat cttcgacacc 241 accggtccgt acatgctgaa aaacaccttc ccggaactgc tgcacggtaa agaagctccg 301 tgctccccgc acatctggga actgtcccgt ttcgctatca actccggtca gaaaggttcc 361 ctgggtttct ccgactgcac cctggaagct atgcgtgctc tggctcgtta ctccttgcag 421 aacgacatcc agaccctggt taccgttacc accgttggtg ttgaaaaaat gatgatccgt 481 gctggtctgg acgtttcccg tttcggtccg cacctgaaaa tcggtatcga acgtgctgtt 541 gctctgcgta tcgaactgaa cgctaaaacc cagatcgctc tgtacggtgg tgttctggtt 601 gaacagcgtc tggctgtttc cgctgctaac gacgaaaact acgctctggt tgcttaataa 661 ctctgatagt gctagtgtag atctctacta gagattaaag aggagaaata ctagatgcgt 721 aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt 781 aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt 841 acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact 901 actttcggtt atggtgttca atgctttgcg agatacccag atcatatgaa acagcatgac 961 tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat 1021 gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga 1081 atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa 1141 tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa 1201 gttaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat 1261 caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc 1321 acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag 1381 tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaata ataatactag 1441 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg 1501 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt 1561 ctgcgtttat a //