LOCUS       BBa_K136063             1513 bp    DNA     linear       23-Dec-2024
DEFINITION  fliL promoter + WT RBS ( K136009) - EnvZ*
ACCESSION   BBa_K136063
VERSION     BBa_K136063.1  
FEATURES             Location/Qualifiers
     misc_feature    127..127
                     /label=transcription initiation site
     misc_feature    44..59
                     /label=FlhDC binding site
     misc_feature    116..116
                     /label=transcription initiation site
     misc_feature    70..85
                     /label=FlhDC binding site
     misc_feature    150..152
                     /label=ATG
     RBS             134..141
                     /label=natural fliL RBS
     promoter        1..154
                     /label=BBa_K136009
     misc_feature    1002..1002
                     /label=I281S T->G
     misc_feature    1511..1513
                     /label=TAA
     misc_feature    161..163
                     /label=ATG
     CDS             161..1513
                     /label=BBa_K136046
ORIGIN
        1 caagggcgtg taacaggcaa cagcggcgtt gatattttcg cctaacgtca gaggtagcac
       61 cgtaatccgc gtcttttccc cgctttgttg cgctcaagac gcaggataat tagccgataa
      121 gcagtagcga cacaggaaga ccgcaacaca tgactactag atgaggcgat tgcgcttctc
      181 gccacgaagt tcatttgccc gtacgttatt gctcatcgtc accttgctgt tcgccagcct
      241 ggtgacgact tatctggtgg tgctgaactt cgcgattttg ccgagcctcc agcagtttaa
      301 taaagtcctc gcgtacgaag tgcgtatgtt gatgaccgac aaactgcaac tggaggacgg
      361 cacgcagttg gttgtgcctc ccgctttccg tcgggagatc taccgtgagc tggggatctc
      421 tctctactcc aacgaggctg ccgaagaggc aggtctgcgt tgggcgcaac actatgaatt
      481 cttaagccat cagatggcgc agcaactggg cggcccgacg gaagtgcgcg ttgaggtcaa
      541 caaaagttcg cctgtcgtct ggctgaaaac ctggctgtcg cccaatatct gggtacgcgt
      601 gccgctgacc gaaattcatc agggcgattt ctctccgctg ttccgctata cgctggcgat
      661 tatgctattg gcgataggcg gggcgtggct gtttattcgt atccagaacc gaccgttggt
      721 cgatctcgaa cacgcagcct tgcaggttgg taaagggatt attccgccgc cgctgcgtga
      781 gtatggcgct tcggaggtgc gttccgttac ccgtgccttt aaccatatgg cggctggtgt
      841 taagcaactg gcggatgacc gcacgctgct gatggcgggg gtaagtcacg acttgcgcac
      901 gccgctgacg cgtattcgcc tggcgactga gatgatgagc gagcaggatg gctatctggc
      961 agaatcgatc aataaagata tcgaagagtg caacgccatc agtgagcagt ttatcgacta
     1021 cctgcgcacc gggcaggaga tgccgatgga aatggcggat cttaatgcag tactcggtga
     1081 ggtgattgct gccgaaagtg gctatgagcg ggaaattgaa accgcgcttt accccggcag
     1141 cattgaagtg aaaatgcacc cgctgtcgat caaacgcgcg gtggcgaata tggtggtcaa
     1201 cgccgcccgt tatggcaatg gctggatcaa agtcagcagc ggaacggagc cgaatcgcgc
     1261 ctggttccag gtggaagatg acggtccggg aattgcgccg gaacaacgta agcacctgtt
     1321 ccagccgttt gtccgcggcg acagtgcgcg caccattagc ggcacgggat tagggctggc
     1381 aattgtgcag cgtatcgtgg ataaccataa cgggatgctg gagcttggca ccagcgagcg
     1441 gggcgggctt tccattcgcg cctggctgcc agtgccggta acgcgggcgc agggcacgac
     1501 aaaagaaggg taa
//
LOCUS       BBa_S04135              1513 bp    DNA     linear       23-Dec-2024
DEFINITION  K136009:K136046
ACCESSION   BBa_S04135
VERSION     BBa_S04135.1  
FEATURES             Location/Qualifiers
     misc_feature    127..127
                     /label=transcription initiation site
     misc_feature    44..59
                     /label=FlhDC binding site
     misc_feature    116..116
                     /label=transcription initiation site
     misc_feature    70..85
                     /label=FlhDC binding site
     misc_feature    150..152
                     /label=ATG
     RBS             134..141
                     /label=natural fliL RBS
     promoter        1..154
                     /label=BBa_K136009
     misc_feature    1002..1002
                     /label=I281S T->G
     misc_feature    1511..1513
                     /label=TAA
     misc_feature    161..163
                     /label=ATG
     CDS             161..1513
                     /label=BBa_K136046
ORIGIN
        1 caagggcgtg taacaggcaa cagcggcgtt gatattttcg cctaacgtca gaggtagcac
       61 cgtaatccgc gtcttttccc cgctttgttg cgctcaagac gcaggataat tagccgataa
      121 gcagtagcga cacaggaaga ccgcaacaca tgactactag atgaggcgat tgcgcttctc
      181 gccacgaagt tcatttgccc gtacgttatt gctcatcgtc accttgctgt tcgccagcct
      241 ggtgacgact tatctggtgg tgctgaactt cgcgattttg ccgagcctcc agcagtttaa
      301 taaagtcctc gcgtacgaag tgcgtatgtt gatgaccgac aaactgcaac tggaggacgg
      361 cacgcagttg gttgtgcctc ccgctttccg tcgggagatc taccgtgagc tggggatctc
      421 tctctactcc aacgaggctg ccgaagaggc aggtctgcgt tgggcgcaac actatgaatt
      481 cttaagccat cagatggcgc agcaactggg cggcccgacg gaagtgcgcg ttgaggtcaa
      541 caaaagttcg cctgtcgtct ggctgaaaac ctggctgtcg cccaatatct gggtacgcgt
      601 gccgctgacc gaaattcatc agggcgattt ctctccgctg ttccgctata cgctggcgat
      661 tatgctattg gcgataggcg gggcgtggct gtttattcgt atccagaacc gaccgttggt
      721 cgatctcgaa cacgcagcct tgcaggttgg taaagggatt attccgccgc cgctgcgtga
      781 gtatggcgct tcggaggtgc gttccgttac ccgtgccttt aaccatatgg cggctggtgt
      841 taagcaactg gcggatgacc gcacgctgct gatggcgggg gtaagtcacg acttgcgcac
      901 gccgctgacg cgtattcgcc tggcgactga gatgatgagc gagcaggatg gctatctggc
      961 agaatcgatc aataaagata tcgaagagtg caacgccatc agtgagcagt ttatcgacta
     1021 cctgcgcacc gggcaggaga tgccgatgga aatggcggat cttaatgcag tactcggtga
     1081 ggtgattgct gccgaaagtg gctatgagcg ggaaattgaa accgcgcttt accccggcag
     1141 cattgaagtg aaaatgcacc cgctgtcgat caaacgcgcg gtggcgaata tggtggtcaa
     1201 cgccgcccgt tatggcaatg gctggatcaa agtcagcagc ggaacggagc cgaatcgcgc
     1261 ctggttccag gtggaagatg acggtccggg aattgcgccg gaacaacgta agcacctgtt
     1321 ccagccgttt gtccgcggcg acagtgcgcg caccattagc ggcacgggat tagggctggc
     1381 aattgtgcag cgtatcgtgg ataaccataa cgggatgctg gagcttggca ccagcgagcg
     1441 gggcgggctt tccattcgcg cctggctgcc agtgccggta acgcgggcgc agggcacgac
     1501 aaaagaaggg taa
//