LOCUS       BBa_K136068             2288 bp    DNA     linear       10-Dec-2025
DEFINITION  flhB Promoter (+ WT RBS) - EnvZ* - GFP generator
ACCESSION   BBa_K136068
VERSION     BBa_K136068.1  
FEATURES             Location/Qualifiers
     misc_feature    79..94
                     /label=flhDC binding site
     misc_feature    106..121
                     /label=flhDC binding site
     misc_feature    153..153
                     /label=transcription initiation site
     misc_feature    178..180
                     /label=GTG
     RBS             166..173
                     /label=natural flhB RBS
     promoter        1..203
                     /label=BBa_K136008
     misc_feature    1051..1051
                     /label=I281S T->G
     misc_feature    1560..1562
                     /label=TAA
     misc_feature    210..212
                     /label=ATG
     CDS             210..1562
                     /label=BBa_K136046
     CDS             1569..2288
                     /label=GFP protein
     CDS             1569..2288
                     /label=BBa_E0040
ORIGIN
        1 ccacgtcata tcaggcggtc tgataaggcg atgacgccgc atccgacaaa cgcacactat
       61 gcttgatgtg tggcagcaaa agccctaaat cccgcctgtt ttgcccctta ctcaaaccat
      121 tgaacgcttt gcgctctggc atcattcacg cttaatactc tttccaggat tggcgacgtg
      181 tctgacgaga gcgacgacaa aactactaga tgaggcgatt gcgcttctcg ccacgaagtt
      241 catttgcccg tacgttattg ctcatcgtca ccttgctgtt cgccagcctg gtgacgactt
      301 atctggtggt gctgaacttc gcgattttgc cgagcctcca gcagtttaat aaagtcctcg
      361 cgtacgaagt gcgtatgttg atgaccgaca aactgcaact ggaggacggc acgcagttgg
      421 ttgtgcctcc cgctttccgt cgggagatct accgtgagct ggggatctct ctctactcca
      481 acgaggctgc cgaagaggca ggtctgcgtt gggcgcaaca ctatgaattc ttaagccatc
      541 agatggcgca gcaactgggc ggcccgacgg aagtgcgcgt tgaggtcaac aaaagttcgc
      601 ctgtcgtctg gctgaaaacc tggctgtcgc ccaatatctg ggtacgcgtg ccgctgaccg
      661 aaattcatca gggcgatttc tctccgctgt tccgctatac gctggcgatt atgctattgg
      721 cgataggcgg ggcgtggctg tttattcgta tccagaaccg accgttggtc gatctcgaac
      781 acgcagcctt gcaggttggt aaagggatta ttccgccgcc gctgcgtgag tatggcgctt
      841 cggaggtgcg ttccgttacc cgtgccttta accatatggc ggctggtgtt aagcaactgg
      901 cggatgaccg cacgctgctg atggcggggg taagtcacga cttgcgcacg ccgctgacgc
      961 gtattcgcct ggcgactgag atgatgagcg agcaggatgg ctatctggca gaatcgatca
     1021 ataaagatat cgaagagtgc aacgccatca gtgagcagtt tatcgactac ctgcgcaccg
     1081 ggcaggagat gccgatggaa atggcggatc ttaatgcagt actcggtgag gtgattgctg
     1141 ccgaaagtgg ctatgagcgg gaaattgaaa ccgcgcttta ccccggcagc attgaagtga
     1201 aaatgcaccc gctgtcgatc aaacgcgcgg tggcgaatat ggtggtcaac gccgcccgtt
     1261 atggcaatgg ctggatcaaa gtcagcagcg gaacggagcc gaatcgcgcc tggttccagg
     1321 tggaagatga cggtccggga attgcgccgg aacaacgtaa gcacctgttc cagccgtttg
     1381 tccgcggcga cagtgcgcgc accattagcg gcacgggatt agggctggca attgtgcagc
     1441 gtatcgtgga taaccataac gggatgctgg agcttggcac cagcgagcgg ggcgggcttt
     1501 ccattcgcgc ctggctgcca gtgccggtaa cgcgggcgca gggcacgaca aaagaagggt
     1561 aatactagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat
     1621 tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa
     1681 catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc
     1741 caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac ccagatcata
     1801 tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta
     1861 tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt gaaggtgata
     1921 cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg
     1981 gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca gacaaacaaa
     2041 agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac
     2101 tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca
     2161 accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca
     2221 tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca
     2281 aataataa
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