LOCUS       BBa_K136069             2397 bp    DNA     linear       25-Nov-2024
DEFINITION  fliL promoter (+WT RBS) - EnvZ*- GFP generator
ACCESSION   BBa_K136069
VERSION     BBa_K136069.1  
FEATURES             Location/Qualifiers
     misc_feature    127..127
                     /label=transcription initiation site
     misc_feature    44..59
                     /label=FlhDC binding site
     misc_feature    116..116
                     /label=transcription initiation site
     misc_feature    70..85
                     /label=FlhDC binding site
     misc_feature    150..152
                     /label=ATG
     RBS             134..141
                     /label=natural fliL RBS
     promoter        1..154
                     /label=BBa_K136009
     misc_feature    1002..1002
                     /label=I281S T->G
     misc_feature    1511..1513
                     /label=TAA
     misc_feature    161..163
                     /label=ATG
     CDS             161..1513
                     /label=BBa_K136046
     RBS             1522..1534
                     /label=RBS-3\Weak
     RBS             1528..1531
                     /label=RBS
     misc_feature    1522..1534
                     /label=BBa_B0032
     RBS             1522..1534
                     /label=BBa_B0032
     CDS             1541..2260
                     /label=GFP protein
     CDS             1541..2260
                     /label=BBa_E0040
     stem_loop       2280..2323
                     /label=stem_loop
     misc_feature    2269..2348
                     /label=BBa_B0010
     terminator      2269..2348
                     /label=BBa_B0010
     stem_loop       2364..2383
                     /label=T7 TE
     misc_feature    2390..2390
                     /label=stop
     polyA_site      2384..2397
                     /label=polya
     misc_feature    2357..2397
                     /label=BBa_B0012
     terminator      2357..2397
                     /label=BBa_B0012
ORIGIN
        1 caagggcgtg taacaggcaa cagcggcgtt gatattttcg cctaacgtca gaggtagcac
       61 cgtaatccgc gtcttttccc cgctttgttg cgctcaagac gcaggataat tagccgataa
      121 gcagtagcga cacaggaaga ccgcaacaca tgactactag atgaggcgat tgcgcttctc
      181 gccacgaagt tcatttgccc gtacgttatt gctcatcgtc accttgctgt tcgccagcct
      241 ggtgacgact tatctggtgg tgctgaactt cgcgattttg ccgagcctcc agcagtttaa
      301 taaagtcctc gcgtacgaag tgcgtatgtt gatgaccgac aaactgcaac tggaggacgg
      361 cacgcagttg gttgtgcctc ccgctttccg tcgggagatc taccgtgagc tggggatctc
      421 tctctactcc aacgaggctg ccgaagaggc aggtctgcgt tgggcgcaac actatgaatt
      481 cttaagccat cagatggcgc agcaactggg cggcccgacg gaagtgcgcg ttgaggtcaa
      541 caaaagttcg cctgtcgtct ggctgaaaac ctggctgtcg cccaatatct gggtacgcgt
      601 gccgctgacc gaaattcatc agggcgattt ctctccgctg ttccgctata cgctggcgat
      661 tatgctattg gcgataggcg gggcgtggct gtttattcgt atccagaacc gaccgttggt
      721 cgatctcgaa cacgcagcct tgcaggttgg taaagggatt attccgccgc cgctgcgtga
      781 gtatggcgct tcggaggtgc gttccgttac ccgtgccttt aaccatatgg cggctggtgt
      841 taagcaactg gcggatgacc gcacgctgct gatggcgggg gtaagtcacg acttgcgcac
      901 gccgctgacg cgtattcgcc tggcgactga gatgatgagc gagcaggatg gctatctggc
      961 agaatcgatc aataaagata tcgaagagtg caacgccatc agtgagcagt ttatcgacta
     1021 cctgcgcacc gggcaggaga tgccgatgga aatggcggat cttaatgcag tactcggtga
     1081 ggtgattgct gccgaaagtg gctatgagcg ggaaattgaa accgcgcttt accccggcag
     1141 cattgaagtg aaaatgcacc cgctgtcgat caaacgcgcg gtggcgaata tggtggtcaa
     1201 cgccgcccgt tatggcaatg gctggatcaa agtcagcagc ggaacggagc cgaatcgcgc
     1261 ctggttccag gtggaagatg acggtccggg aattgcgccg gaacaacgta agcacctgtt
     1321 ccagccgttt gtccgcggcg acagtgcgcg caccattagc ggcacgggat tagggctggc
     1381 aattgtgcag cgtatcgtgg ataaccataa cgggatgctg gagcttggca ccagcgagcg
     1441 gggcgggctt tccattcgcg cctggctgcc agtgccggta acgcgggcgc agggcacgac
     1501 aaaagaaggg taatactaga gtcacacagg aaagtactag atgcgtaaag gagaagaact
     1561 tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt
     1621 ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat
     1681 ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt tcggttatgg
     1741 tgttcaatgc tttgcgagat acccagatca tatgaaacag catgactttt tcaagagtgc
     1801 catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa
     1861 gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg
     1921 tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc
     1981 acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagtta acttcaaaat
     2041 tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc
     2101 aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct
     2161 ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc
     2221 tgggattaca catggcatgg atgaactata caaataataa tactagagcc aggcatcaaa
     2281 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     2341 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//