##gff-version 3
##sequence-region BBa_K136069 1 2397
BBa_K136069	.	terminator	2357	2397	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K136069	.	engineered_region	2357	2397	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K136069	.	stop_codon	2390	2390	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K136069	.	polyA_site	2384	2397	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K136069	.	stem_loop	2364	2383	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K136069	.	CDS	1541	2260	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_K136069	.	CDS	1541	2260	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K136069	.	terminator	2269	2348	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K136069	.	stem_loop	2280	2323	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K136069	.	engineered_region	2269	2348	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K136069	.	ribosome_entry_site	1522	1534	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K136069	.	ribosome_entry_site	1528	1531	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K136069	.	ribosome_entry_site	1522	1534	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K136069	.	engineered_region	1522	1534	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K136069	.	CDS	161	1513	.	+	0	ID=BBa_K136046;Name=BBa_K136046
BBa_K136069	.	stop_codon	1511	1513	.	+	0	ID=annotation1994303;Name=TAA;Parent=BBa_K136046
BBa_K136069	.	point_mutation	1002	1002	.	+	0	ID=annotation1994273;Name=I281S T->G;Parent=BBa_K136046
BBa_K136069	.	start_codon	161	163	.	+	0	ID=annotation1994304;Name=ATG;Parent=BBa_K136046
BBa_K136069	.	promoter	1	154	.	+	0	ID=BBa_K136009;Name=BBa_K136009
BBa_K136069	.	sequence_feature	116	116	.	+	0	ID=annotation1987003;Name=transcription initiation site;Parent=BBa_K136009
BBa_K136069	.	start_codon	150	152	.	+	0	ID=annotation1999343;Name=ATG;Parent=BBa_K136009
BBa_K136069	.	non_covalent_binding_site	44	59	.	+	0	ID=annotation1987002;Name=FlhDC binding site;Parent=BBa_K136009
BBa_K136069	.	sequence_feature	127	127	.	+	0	ID=annotation1987001;Name=transcription initiation site;Parent=BBa_K136009
BBa_K136069	.	non_covalent_binding_site	70	85	.	+	0	ID=annotation1987004;Name=FlhDC binding site;Parent=BBa_K136009
BBa_K136069	.	ribosome_entry_site	134	141	.	+	0	ID=annotation1999344;Name=natural fliL RBS;Parent=BBa_K136009
>BBa_K136069
caagggcgtgtaacaggcaacagcggcgttgatattttcgcctaacgtcagaggtagcaccgtaatccgcgtcttttcc
ccgctttgttgcgctcaagacgcaggataattagccgataagcagtagcgacacaggaagaccgcaacacatgactact
agatgaggcgattgcgcttctcgccacgaagttcatttgcccgtacgttattgctcatcgtcaccttgctgttcgccag
cctggtgacgacttatctggtggtgctgaacttcgcgattttgccgagcctccagcagtttaataaagtcctcgcgtac
gaagtgcgtatgttgatgaccgacaaactgcaactggaggacggcacgcagttggttgtgcctcccgctttccgtcggg
agatctaccgtgagctggggatctctctctactccaacgaggctgccgaagaggcaggtctgcgttgggcgcaacacta
tgaattcttaagccatcagatggcgcagcaactgggcggcccgacggaagtgcgcgttgaggtcaacaaaagttcgcct
gtcgtctggctgaaaacctggctgtcgcccaatatctgggtacgcgtgccgctgaccgaaattcatcagggcgatttct
ctccgctgttccgctatacgctggcgattatgctattggcgataggcggggcgtggctgtttattcgtatccagaaccg
accgttggtcgatctcgaacacgcagccttgcaggttggtaaagggattattccgccgccgctgcgtgagtatggcgct
tcggaggtgcgttccgttacccgtgcctttaaccatatggcggctggtgttaagcaactggcggatgaccgcacgctgc
tgatggcgggggtaagtcacgacttgcgcacgccgctgacgcgtattcgcctggcgactgagatgatgagcgagcagga
tggctatctggcagaatcgatcaataaagatatcgaagagtgcaacgccatcagtgagcagtttatcgactacctgcgc
accgggcaggagatgccgatggaaatggcggatcttaatgcagtactcggtgaggtgattgctgccgaaagtggctatg
agcgggaaattgaaaccgcgctttaccccggcagcattgaagtgaaaatgcacccgctgtcgatcaaacgcgcggtggc
gaatatggtggtcaacgccgcccgttatggcaatggctggatcaaagtcagcagcggaacggagccgaatcgcgcctgg
ttccaggtggaagatgacggtccgggaattgcgccggaacaacgtaagcacctgttccagccgtttgtccgcggcgaca
gtgcgcgcaccattagcggcacgggattagggctggcaattgtgcagcgtatcgtggataaccataacgggatgctgga
gcttggcaccagcgagcggggcgggctttccattcgcgcctggctgccagtgccggtaacgcgggcgcagggcacgaca
aaagaagggtaatactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaa
ttcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacgg
aaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttat
ggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatg
tacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgataccct
tgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactat
aactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattg
aagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaa
ccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgta
acagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaag
gctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcac
cttcgggtgggcctttctgcgtttata