BBa_K136069
type: ComponentDefinition
persistent identity: BBa K136069
display id: BBa_K136069
version: 1
was generated by: iGEM to SBOL conversion
description: fliL promoter (+WT RBS) - EnvZ*- GFP generator
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:03Z
mutable provenance: none
owned by: james
owned by: myers
mutable description: none
discontinued: false
dominant: false
group u list: _211_
m user id: 0
owner id: 3200
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Beno??t D'hayer
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K136046
component: BBa_K136009
component: BBa_E0040
component: BBa_B0032
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_K136009
sequence annotation: BBa_K136046
sequence annotation: BBa_B0032
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K136069_sequence/1

BBa_K136046
type: Component
persistent identity: component1997255
display id: component1997255
version: 1
top level: BBa_K136069
definition: BBa_K136046
access: public

BBa_K136009
type: Component
persistent identity: component1997251
display id: component1997251
version: 1
top level: BBa_K136069
definition: BBa_K136009
access: public

BBa_E0040
type: Component
persistent identity: component1997260
display id: component1997260
version: 1
top level: BBa_K136069
definition: GFP
access: public

BBa_B0032
type: Component
persistent identity: component1997257
display id: component1997257
version: 1
top level: BBa_K136069
definition: BBa_B0032
access: public

BBa_B0012
type: Component
persistent identity: component1997263
display id: component1997263
version: 1
top level: BBa_K136069
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1997261
display id: component1997261
version: 1
top level: BBa_K136069
definition: BBa_B0010
access: public

BBa_K136009
type: SequenceAnnotation
persistent identity: annotation1997251
display id: annotation1997251
version: 1
top level: BBa_K136069
location: https://synbiohub.org/public/igem/BBa_K136069/annotation1997251/range1997251/1
component: BBa_K136009

https://synbiohub.org/public/igem/BBa_K136069/annotation1997251/range1997251/1
type: Range
persistent identity: range1997251
display id: range1997251
version: 1
top level: BBa_K136069
direction: direction/reverse
start: 1
end: 154
orientation: inline

BBa_K136046
type: SequenceAnnotation
persistent identity: annotation1997255
display id: annotation1997255
version: 1
top level: BBa_K136069
location: https://synbiohub.org/public/igem/BBa_K136069/annotation1997255/range1997255/1
component: BBa_K136046

https://synbiohub.org/public/igem/BBa_K136069/annotation1997255/range1997255/1
type: Range
persistent identity: range1997255
display id: range1997255
version: 1
top level: BBa_K136069
direction: direction/reverse
start: 161
end: 1513
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1997257
display id: annotation1997257
version: 1
top level: BBa_K136069
location: https://synbiohub.org/public/igem/BBa_K136069/annotation1997257/range1997257/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K136069/annotation1997257/range1997257/1
type: Range
persistent identity: range1997257
display id: range1997257
version: 1
top level: BBa_K136069
direction: direction/forward
start: 1522
end: 1534
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1997260
display id: annotation1997260
version: 1
top level: BBa_K136069
location: https://synbiohub.org/public/igem/BBa_K136069/annotation1997260/range1997260/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K136069/annotation1997260/range1997260/1
type: Range
persistent identity: range1997260
display id: range1997260
version: 1
top level: BBa_K136069
direction: direction/reverse
start: 1541
end: 2260
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1997261
display id: annotation1997261
version: 1
top level: BBa_K136069
location: https://synbiohub.org/public/igem/BBa_K136069/annotation1997261/range1997261/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K136069/annotation1997261/range1997261/1
type: Range
persistent identity: range1997261
display id: range1997261
version: 1
top level: BBa_K136069
direction: direction/forward
start: 2269
end: 2348
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1997263
display id: annotation1997263
version: 1
top level: BBa_K136069
location: https://synbiohub.org/public/igem/BBa_K136069/annotation1997263/range1997263/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K136069/annotation1997263/range1997263/1
type: Range
persistent identity: range1997263
display id: range1997263
version: 1
top level: BBa_K136069
direction: direction/forward
start: 2357
end: 2397
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_K136009
type: ComponentDefinition
persistent identity: BBa K136009
display id: BBa_K136009
version: 1
was generated by: iGEM to SBOL conversion
description: fliL promoter followed by its natural RBS
created: 2008-10-24T11:00:00Z
modified: 2015-05-08T01:10:02Z
mutable provenance: This part comes from the genome of E.coli K12 strain MG1655.
owned by: james
owned by: myers
mutable description: Promoter of fliL gene from E. coli flagella fliL is a class 2 gene that is part of the construction of the flagella ( Ordering Genes in a Flagella Pathway by Analysis of Expression Kinetics from Living Bacteria - S. Kalir, J. McClure, K. Pabbaraju, C. Southward, M. Ronen, S. Leibler, M. G. Surette, U. Alon, ). Its promoter is consitutively repressed. It is activated by flhD4C2 and by fliA with different forces ( Using a Quantitative Blueprint to Reprogram the Dynamics of the Flagella Gene Network - Shiraz Kalir and Uri Alon ).
discontinued: false
dominant: false
group u list: _211_
m user id: 0
owner id: 3262
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: >forward primer TCGAATTCGCGGCCGCTTCTAGAGCAAGGGCGTGTAACAGGCAAC >reverse primer TCCTGCAGCGGCCGCTACTAGTAGTCATGTGTTGCGGTCTTCCTGTG
star: false
creator: Kok-Phen YAN
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: FlhDC binding site
sequence annotation: transcription initiation site
sequence annotation: natural fliL RBS
sequence annotation: ATG
sequence: https://synbiohub.org/public/igem/BBa_K136009_sequence/1

FlhDC binding site
type: SequenceAnnotation
persistent identity: annotation1987002
display id: annotation1987002
version: 1
top level: BBa_K136009
location: https://synbiohub.org/public/igem/BBa_K136009/annotation1987002/range1987002/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K136009/annotation1987002/range1987002/1
type: Range
persistent identity: range1987002
display id: range1987002
version: 1
top level: BBa_K136009
direction: direction/forward
start: 44
end: 59
orientation: inline

FlhDC binding site
type: SequenceAnnotation
persistent identity: annotation1987004
display id: annotation1987004
version: 1
top level: BBa_K136009
location: https://synbiohub.org/public/igem/BBa_K136009/annotation1987004/range1987004/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K136009/annotation1987004/range1987004/1
type: Range
persistent identity: range1987004
display id: range1987004
version: 1
top level: BBa_K136009
direction: direction/forward
start: 70
end: 85
orientation: inline

transcription initiation site
type: SequenceAnnotation
persistent identity: annotation1987003
display id: annotation1987003
version: 1
top level: BBa_K136009
location: https://synbiohub.org/public/igem/BBa_K136009/annotation1987003/range1987003/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K136009/annotation1987003/range1987003/1
type: Range
persistent identity: range1987003
display id: range1987003
version: 1
top level: BBa_K136009
direction: direction/forward
start: 116
end: 116
orientation: inline

transcription initiation site
type: SequenceAnnotation
persistent identity: annotation1987001
display id: annotation1987001
version: 1
top level: BBa_K136009
location: https://synbiohub.org/public/igem/BBa_K136009/annotation1987001/range1987001/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K136009/annotation1987001/range1987001/1
type: Range
persistent identity: range1987001
display id: range1987001
version: 1
top level: BBa_K136009
direction: direction/forward
start: 127
end: 127
orientation: inline

natural fliL RBS
type: SequenceAnnotation
persistent identity: annotation1999344
display id: annotation1999344
version: 1
top level: BBa_K136009
location: https://synbiohub.org/public/igem/BBa_K136009/annotation1999344/range1999344/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K136009/annotation1999344/range1999344/1
type: Range
persistent identity: range1999344
display id: range1999344
version: 1
top level: BBa_K136009
direction: direction/forward
start: 134
end: 141
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation1999343
display id: annotation1999343
version: 1
top level: BBa_K136009
location: https://synbiohub.org/public/igem/BBa_K136009/annotation1999343/range1999343/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K136009/annotation1999343/range1999343/1
type: Range
persistent identity: range1999343
display id: range1999343
version: 1
top level: BBa_K136009
direction: direction/forward
start: 150
end: 152
orientation: inline

BBa_K136046
type: ComponentDefinition
persistent identity: BBa K136046
display id: BBa_K136046
version: 1
was generated by: iGEM to SBOL conversion
description: envZ*
created: 2008-10-24T11:00:00Z
modified: 2015-05-08T01:10:03Z
mutable provenance: bacterial genome of mutated (I281S) strain for EnvZ.
owned by: james
owned by: myers
mutable description: EnvZ* is a transmenbrane kinase that phosphorylates ompR the natural inhibitor of pFlhDC independently of any outer signal.
discontinued: false
dominant: false
group u list: _211_
m user id: 0
owner id: 3215
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: PCR and ligation in pCR4Blunt-TOPO
star: false
creator: alexandra bouaziz
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: ATG
sequence annotation: I281S T->G
sequence annotation: TAA
sequence: https://synbiohub.org/public/igem/BBa_K136046_sequence/1

ATG
type: SequenceAnnotation
persistent identity: annotation1994304
display id: annotation1994304
version: 1
top level: BBa_K136046
location: https://synbiohub.org/public/igem/BBa_K136046/annotation1994304/range1994304/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K136046/annotation1994304/range1994304/1
type: Range
persistent identity: range1994304
display id: range1994304
version: 1
top level: BBa_K136046
direction: direction/forward
start: 1
end: 3
orientation: inline

I281S T->G
type: SequenceAnnotation
persistent identity: annotation1994273
display id: annotation1994273
version: 1
top level: BBa_K136046
location: https://synbiohub.org/public/igem/BBa_K136046/annotation1994273/range1994273/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_K136046/annotation1994273/range1994273/1
type: Range
persistent identity: range1994273
display id: range1994273
version: 1
top level: BBa_K136046
direction: direction/forward
start: 842
end: 842
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation1994303
display id: annotation1994303
version: 1
top level: BBa_K136046
location: https://synbiohub.org/public/igem/BBa_K136046/annotation1994303/range1994303/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K136046/annotation1994303/range1994303/1
type: Range
persistent identity: range1994303
display id: range1994303
version: 1
top level: BBa_K136046
direction: direction/forward
start: 1351
end: 1353
orientation: inline

https://synbiohub.org/public/igem/BBa_K136069_sequence/1
type: Sequence
persistent identity: BBa K136069 sequence
display id: BBa_K136069_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caagggcgtgtaacaggcaacagcggcgttgatattttcgcctaacgtcagaggtagcaccgtaatccgcgtcttttccccgctttgttgcgctcaagacgcaggataattagccgataagcagtagcgacacaggaagaccgcaacacatgactactagatgaggcgattgcgcttctcgccacgaagttcatttgcccgtacgttattgctcatcgtcaccttgctgttcgccagcctggtgacgacttatctggtggtgctgaacttcgcgattttgccgagcctccagcagtttaataaagtcctcgcgtacgaagtgcgtatgttgatgaccgacaaactgcaactggaggacggcacgcagttggttgtgcctcccgctttccgtcgggagatctaccgtgagctggggatctctctctactccaacgaggctgccgaagaggcaggtctgcgttgggcgcaacactatgaattcttaagccatcagatggcgcagcaactgggcggcccgacggaagtgcgcgttgaggtcaacaaaagttcgcctgtcgtctggctgaaaacctggctgtcgcccaatatctgggtacgcgtgccgctgaccgaaattcatcagggcgatttctctccgctgttccgctatacgctggcgattatgctattggcgataggcggggcgtggctgtttattcgtatccagaaccgaccgttggtcgatctcgaacacgcagccttgcaggttggtaaagggattattccgccgccgctgcgtgagtatggcgcttcggaggtgcgttccgttacccgtgcctttaaccatatggcggctggtgttaagcaactggcggatgaccgcacgctgctgatggcgggggtaagtcacgacttgcgcacgccgctgacgcgtattcgcctggcgactgagatgatgagcgagcaggatggctatctggcagaatcgatcaataaagatatcgaagagtgcaacgccatcagtgagcagtttatcgactacctgcgcaccgggcaggagatgccgatggaaatggcggatcttaatgcagtactcggtgaggtgattgctgccgaaagtggctatgagcgggaaattgaaaccgcgctttaccccggcagcattgaagtgaaaatgcacccgctgtcgatcaaacgcgcggtggcgaatatggtggtcaacgccgcccgttatggcaatggctggatcaaagtcagcagcggaacggagccgaatcgcgcctggttccaggtggaagatgacggtccgggaattgcgccggaacaacgtaagcacctgttccagccgtttgtccgcggcgacagtgcgcgcaccattagcggcacgggattagggctggcaattgtgcagcgtatcgtggataaccataacgggatgctggagcttggcaccagcgagcggggcgggctttccattcgcgcctggctgccagtgccggtaacgcgggcgcagggcacgacaaaagaagggtaatactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatgg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K136009_sequence/1
type: Sequence
persistent identity: BBa K136009 sequence
display id: BBa_K136009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caagggcgtgtaacaggcaacagcggcgttgatattttcgcctaacgtcagaggtagcaccgtaatccgcgtcttttccccgctttgttgcgctcaagacgcaggataattagccgataagcagtagcgacacaggaagaccgcaacacatgac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K136046_sequence/1
type: Sequence
persistent identity: BBa K136046 sequence
display id: BBa_K136046_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaggcgattgcgcttctcgccacgaagttcatttgcccgtacgttattgctcatcgtcaccttgctgttcgccagcctggtgacgacttatctggtggtgctgaacttcgcgattttgccgagcctccagcagtttaataaagtcctcgcgtacgaagtgcgtatgttgatgaccgacaaactgcaactggaggacggcacgcagttggttgtgcctcccgctttccgtcgggagatctaccgtgagctggggatctctctctactccaacgaggctgccgaagaggcaggtctgcgttgggcgcaacactatgaattcttaagccatcagatggcgcagcaactgggcggcccgacggaagtgcgcgttgaggtcaacaaaagttcgcctgtcgtctggctgaaaacctggctgtcgcccaatatctgggtacgcgtgccgctgaccgaaattcatcagggcgatttctctccgctgttccgctatacgctggcgattatgctattggcgataggcggggcgtggctgtttattcgtatccagaaccgaccgttggtcgatctcgaacacgcagccttgcaggttggtaaagggattattccgccgccgctgcgtgagtatggcgcttcggaggtgcgttccgttacccgtgcctttaaccatatggcggctggtgttaagcaactggcggatgaccgcacgctgctgatggcgggggtaagtcacgacttgcgcacgccgctgacgcgtattcgcctggcgactgagatgatgagcgagcaggatggctatctggcagaatcgatcaataaagatatcgaagagtgcaacgccatcagtgagcagtttatcgactacctgcgcaccgggcaggagatgccgatggaaatggcggatcttaatgcagtactcggtgaggtgattgctgccgaaagtggctatgagcgggaaattgaaaccgcgctttaccccggcagcattgaagtgaaaatgcacccgctgtcgatcaaacgcgcggtggcgaatatggtggtcaacgccgcccgttatggcaatggctggatcaaagtcagcagcggaacggagccgaatcgcgcctggttccaggtggaagatgacggtccgggaattgcgccggaacaacgtaagcacctgttccagccgtttgtccgcggcgacagtgcgcgcaccattagcggcacgggattagggctggcaattgtgcagcgtatcgtggataaccataacgggatgctggagcttggcaccagcgagcggggcgggctttccattcgcgcctggctgccagtgccggtaacgcgggcgcagggcacgacaaaagaagggtaa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z