LOCUS       BBa_K136070             2446 bp    DNA     linear       18-Feb-2025
DEFINITION  flhB Promoter (+ WT RBS) - EnvZ* - GFP generator
ACCESSION   BBa_K136070
VERSION     BBa_K136070.1  
FEATURES             Location/Qualifiers
     misc_feature    79..94
                     /label=flhDC binding site
     misc_feature    106..121
                     /label=flhDC binding site
     misc_feature    153..153
                     /label=transcription initiation site
     misc_feature    178..180
                     /label=GTG
     RBS             166..173
                     /label=natural flhB RBS
     promoter        1..203
                     /label=BBa_K136008
     misc_feature    1051..1051
                     /label=I281S T->G
     misc_feature    1560..1562
                     /label=TAA
     misc_feature    210..212
                     /label=ATG
     CDS             210..1562
                     /label=BBa_K136046
     RBS             1571..1583
                     /label=RBS-3\Weak
     RBS             1577..1580
                     /label=RBS
     misc_feature    1571..1583
                     /label=BBa_B0032
     RBS             1571..1583
                     /label=BBa_B0032
     CDS             1590..2309
                     /label=GFP protein
     CDS             1590..2309
                     /label=BBa_E0040
     stem_loop       2329..2372
                     /label=stem_loop
     misc_feature    2318..2397
                     /label=BBa_B0010
     terminator      2318..2397
                     /label=BBa_B0010
     stem_loop       2413..2432
                     /label=T7 TE
     misc_feature    2439..2439
                     /label=stop
     polyA_site      2433..2446
                     /label=polya
     misc_feature    2406..2446
                     /label=BBa_B0012
     terminator      2406..2446
                     /label=BBa_B0012
ORIGIN
        1 ccacgtcata tcaggcggtc tgataaggcg atgacgccgc atccgacaaa cgcacactat
       61 gcttgatgtg tggcagcaaa agccctaaat cccgcctgtt ttgcccctta ctcaaaccat
      121 tgaacgcttt gcgctctggc atcattcacg cttaatactc tttccaggat tggcgacgtg
      181 tctgacgaga gcgacgacaa aactactaga tgaggcgatt gcgcttctcg ccacgaagtt
      241 catttgcccg tacgttattg ctcatcgtca ccttgctgtt cgccagcctg gtgacgactt
      301 atctggtggt gctgaacttc gcgattttgc cgagcctcca gcagtttaat aaagtcctcg
      361 cgtacgaagt gcgtatgttg atgaccgaca aactgcaact ggaggacggc acgcagttgg
      421 ttgtgcctcc cgctttccgt cgggagatct accgtgagct ggggatctct ctctactcca
      481 acgaggctgc cgaagaggca ggtctgcgtt gggcgcaaca ctatgaattc ttaagccatc
      541 agatggcgca gcaactgggc ggcccgacgg aagtgcgcgt tgaggtcaac aaaagttcgc
      601 ctgtcgtctg gctgaaaacc tggctgtcgc ccaatatctg ggtacgcgtg ccgctgaccg
      661 aaattcatca gggcgatttc tctccgctgt tccgctatac gctggcgatt atgctattgg
      721 cgataggcgg ggcgtggctg tttattcgta tccagaaccg accgttggtc gatctcgaac
      781 acgcagcctt gcaggttggt aaagggatta ttccgccgcc gctgcgtgag tatggcgctt
      841 cggaggtgcg ttccgttacc cgtgccttta accatatggc ggctggtgtt aagcaactgg
      901 cggatgaccg cacgctgctg atggcggggg taagtcacga cttgcgcacg ccgctgacgc
      961 gtattcgcct ggcgactgag atgatgagcg agcaggatgg ctatctggca gaatcgatca
     1021 ataaagatat cgaagagtgc aacgccatca gtgagcagtt tatcgactac ctgcgcaccg
     1081 ggcaggagat gccgatggaa atggcggatc ttaatgcagt actcggtgag gtgattgctg
     1141 ccgaaagtgg ctatgagcgg gaaattgaaa ccgcgcttta ccccggcagc attgaagtga
     1201 aaatgcaccc gctgtcgatc aaacgcgcgg tggcgaatat ggtggtcaac gccgcccgtt
     1261 atggcaatgg ctggatcaaa gtcagcagcg gaacggagcc gaatcgcgcc tggttccagg
     1321 tggaagatga cggtccggga attgcgccgg aacaacgtaa gcacctgttc cagccgtttg
     1381 tccgcggcga cagtgcgcgc accattagcg gcacgggatt agggctggca attgtgcagc
     1441 gtatcgtgga taaccataac gggatgctgg agcttggcac cagcgagcgg ggcgggcttt
     1501 ccattcgcgc ctggctgcca gtgccggtaa cgcgggcgca gggcacgaca aaagaagggt
     1561 aatactagag tcacacagga aagtactaga tgcgtaaagg agaagaactt ttcactggag
     1621 ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg
     1681 gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg
     1741 gaaaactacc tgttccatgg ccaacacttg tcactacttt cggttatggt gttcaatgct
     1801 ttgcgagata cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag
     1861 gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg
     1921 aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta
     1981 aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat
     2041 acatcatggc agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca
     2101 ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg
     2161 gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc
     2221 ccaacgaaaa gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac
     2281 atggcatgga tgaactatac aaataataat actagagcca ggcatcaaat aaaacgaaag
     2341 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
     2401 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//