##gff-version 3
##sequence-region BBa_K136070 1 2446
BBa_K136070	.	terminator	2318	2397	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K136070	.	stem_loop	2329	2372	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K136070	.	engineered_region	2318	2397	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K136070	.	CDS	210	1562	.	+	0	ID=BBa_K136046;Name=BBa_K136046
BBa_K136070	.	start_codon	210	212	.	+	0	ID=annotation1994304;Name=ATG;Parent=BBa_K136046
BBa_K136070	.	stop_codon	1560	1562	.	+	0	ID=annotation1994303;Name=TAA;Parent=BBa_K136046
BBa_K136070	.	point_mutation	1051	1051	.	+	0	ID=annotation1994273;Name=I281S T->G;Parent=BBa_K136046
BBa_K136070	.	terminator	2406	2446	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K136070	.	polyA_site	2433	2446	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K136070	.	engineered_region	2406	2446	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K136070	.	stem_loop	2413	2432	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K136070	.	stop_codon	2439	2439	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K136070	.	ribosome_entry_site	1571	1583	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K136070	.	engineered_region	1571	1583	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K136070	.	ribosome_entry_site	1571	1583	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K136070	.	ribosome_entry_site	1577	1580	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K136070	.	promoter	1	203	.	+	0	ID=BBa_K136008;Name=BBa_K136008
BBa_K136070	.	non_covalent_binding_site	106	121	.	+	0	ID=annotation1986995;Name=flhDC binding site;Parent=BBa_K136008
BBa_K136070	.	non_covalent_binding_site	79	94	.	+	0	ID=annotation1986994;Name=flhDC binding site;Parent=BBa_K136008
BBa_K136070	.	start_codon	178	180	.	+	0	ID=annotation1999345;Name=GTG;Parent=BBa_K136008
BBa_K136070	.	ribosome_entry_site	166	173	.	+	0	ID=annotation1999346;Name=natural flhB RBS;Parent=BBa_K136008
BBa_K136070	.	sequence_feature	153	153	.	+	0	ID=annotation1986996;Name=transcription initiation site;Parent=BBa_K136008
BBa_K136070	.	CDS	1590	2309	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_K136070	.	CDS	1590	2309	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
>BBa_K136070
ccacgtcatatcaggcggtctgataaggcgatgacgccgcatccgacaaacgcacactatgcttgatgtgtggcagcaa
aagccctaaatcccgcctgttttgccccttactcaaaccattgaacgctttgcgctctggcatcattcacgcttaatac
tctttccaggattggcgacgtgtctgacgagagcgacgacaaaactactagatgaggcgattgcgcttctcgccacgaa
gttcatttgcccgtacgttattgctcatcgtcaccttgctgttcgccagcctggtgacgacttatctggtggtgctgaa
cttcgcgattttgccgagcctccagcagtttaataaagtcctcgcgtacgaagtgcgtatgttgatgaccgacaaactg
caactggaggacggcacgcagttggttgtgcctcccgctttccgtcgggagatctaccgtgagctggggatctctctct
actccaacgaggctgccgaagaggcaggtctgcgttgggcgcaacactatgaattcttaagccatcagatggcgcagca
actgggcggcccgacggaagtgcgcgttgaggtcaacaaaagttcgcctgtcgtctggctgaaaacctggctgtcgccc
aatatctgggtacgcgtgccgctgaccgaaattcatcagggcgatttctctccgctgttccgctatacgctggcgatta
tgctattggcgataggcggggcgtggctgtttattcgtatccagaaccgaccgttggtcgatctcgaacacgcagcctt
gcaggttggtaaagggattattccgccgccgctgcgtgagtatggcgcttcggaggtgcgttccgttacccgtgccttt
aaccatatggcggctggtgttaagcaactggcggatgaccgcacgctgctgatggcgggggtaagtcacgacttgcgca
cgccgctgacgcgtattcgcctggcgactgagatgatgagcgagcaggatggctatctggcagaatcgatcaataaaga
tatcgaagagtgcaacgccatcagtgagcagtttatcgactacctgcgcaccgggcaggagatgccgatggaaatggcg
gatcttaatgcagtactcggtgaggtgattgctgccgaaagtggctatgagcgggaaattgaaaccgcgctttaccccg
gcagcattgaagtgaaaatgcacccgctgtcgatcaaacgcgcggtggcgaatatggtggtcaacgccgcccgttatgg
caatggctggatcaaagtcagcagcggaacggagccgaatcgcgcctggttccaggtggaagatgacggtccgggaatt
gcgccggaacaacgtaagcacctgttccagccgtttgtccgcggcgacagtgcgcgcaccattagcggcacgggattag
ggctggcaattgtgcagcgtatcgtggataaccataacgggatgctggagcttggcaccagcgagcggggcgggctttc
cattcgcgcctggctgccagtgccggtaacgcgggcgcagggcacgacaaaagaagggtaatactagagtcacacagga
aagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatg
ggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactac
tggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagat
catatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatg
acgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattga
ttttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagac
aaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccatt
atcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttc
gaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggat
gaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttt
atctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata