LOCUS       BBa_K1361002            1609 bp    DNA     linear       15-Jun-2024
DEFINITION  Curli Fiber generator where CsgBtrunc, a dissociative nucleator, 
            under the control of Pbad promoter
ACCESSION   BBa_K1361002
VERSION     BBa_K1361002.1  
FEATURES             Location/Qualifiers
     promoter        1..131
                     /label=promoter
     misc_feature    40..57
                     /label=AraI1
     misc_feature    61..78
                     /label=AraI2
     promoter        1..130
                     /label=BBa_K206000
     misc_feature    143..146
                     /label=conserved
     RBS             139..150
                     /label=BBa_B0034
     CDS             157..555
                     /label=cds
     CDS             157..555
                     /label=BBa_K1361995
     stem_loop       575..618
                     /label=stem_loop
     misc_feature    564..643
                     /label=BBa_B0010
     terminator      564..643
                     /label=BBa_B0010
     stem_loop       659..678
                     /label=T7 TE
     misc_feature    685..685
                     /label=stop
     polyA_site      679..692
                     /label=polya
     misc_feature    652..692
                     /label=BBa_B0012
     terminator      652..692
                     /label=BBa_B0012
     terminator      564..692
                     /label=BBa_B0015
     promoter        701..735
                     /label=BBa_J23104
     RBS             744..756
                     /label=RBS-3\Weak
     RBS             750..753
                     /label=RBS
     misc_feature    744..756
                     /label=BBa_B0032
     RBS             744..756
                     /label=BBa_B0032
     misc_feature    701..756
                     /label=BBa_K608003
     CDS             763..1218
                     /label=CsgA
     misc_feature    763..765
                     /label=start
     misc_feature    1216..1218
                     /label=stop
     CDS             1277..1609
                     /label=CsgC
     misc_feature    1277..1279
                     /label=start
     misc_feature    1607..1609
                     /label=stop
     misc_feature    763..1609
                     /label=BBa_K1361998
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcaagatag tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagaa agaggagaaa tactagatga aaaacaaatt gttatttatg
      181 atgttaacaa tactgggtgc gcctgggatt gcagccgcag caggttatga tttagctaat
      241 tcagaatata acttcgcggt aaatgaattg agtaagtctt catttaatca ggcagccata
      301 attggtcaag ctgggactaa taatagtgct cagttacggc agggaggctc aaaacttttg
      361 gcggttgttg cgcaagaagg tagtagcaac cgggcaaaga ttgaccagac aggagattat
      421 aaccttgcat atattgatca ggcgggcagt gccaacgatg ccagtatttc gcaaggtgct
      481 tatggtaata ctgcgatgat tatccagaaa ggttctggta ataaagcaaa tattacacag
      541 tatggtactc aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
      601 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
      661 ctcaccttcg ggtgggcctt tctgcgttta tatactagag ttgacagcta gctcagtcct
      721 aggtattgtg ctagctacta gagtcacaca ggaaagtact agatgaaact tttaaaagta
      781 gcagcaattg cagcaatcgt attctccggt agcgctctgg caggtgttgt tcctcagtac
      841 ggcggcggcg gtaaccacgg tggtggcggt aataatagcg gcccaaattc tgagctgaac
      901 atttaccagt acggtggcgg taactctgca cttgctctgc aaactgatgc ccgtaactct
      961 gacttgacta ttacccagca tggcggcggt aatggtgcag atgttggtca gggctcagat
     1021 gacagctcaa tcgatctgac ccaacgtggc ttcggtaaca gcgctactct tgatcagtgg
     1081 aacggcaaaa attctgaaat gacggttaaa cagttcggtg gtggcaacgg tgccgcagtt
     1141 gaccagactg catctaactc ctccgtcaac gtgactcagg ttggctttgg taacaacgcg
     1201 accgctcatc agtactaata catcatttgt attacagaaa cagggcgcaa gccctgtttt
     1261 ttttcgggag aagaatatga atacgttatt actccttgcg gcactttcca gtcagataac
     1321 ctttaatacg acccagcaag gggatgtgta taccattatt cctgaagtca ctcttactca
     1381 atcttgtctg tgcagagtac aaatattgtc cctgcgcgaa ggcagttcag ggcaaagtca
     1441 gacgaagcaa gaaaagaccc tttcattgcc tgctaatcaa cccattgctt tgacgaagtt
     1501 gagtttaaat atttccccgg acgatcgggt gaaaatagtt gttactgttt ctgatggaca
     1561 gtcacttcat ttatcacaac aatggccgcc ctcttcagaa aagtcttaa
//