BBa_K1361002
type: ComponentDefinition
persistent identity: BBa K1361002
display id: BBa_K1361002
version: 1
was generated by: iGEM to SBOL conversion
description: Curli Fiber generator where CsgBtrunc, a dissociative nucleator, under the control of Pbad promoter
created: 2014-10-03T11:00:00Z
modified: 2015-05-08T01:10:03Z
mutable provenance: CsgAC and CsgBtrunc were collected by PCR from genome of E coli DH5alpha respectively.
owned by: james
owned by: myers
mutable description: This part contains the major subunit of curli fiber CsgA and its nucleator CsgBtrunc, in which CsgA is constitutive expression whereas CsgBtrunc is under the control of T7 promotor. This part cannot functioning alone without expression of CsgEFG genes in the same cell, for they coding the outer membrane channel secrete system for CsgA and CsgB.
discontinued: false
dominant: false
experience: experience/None
group u list: _1737_
m user id: 0
owner id: 18617
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: The PstI loci within CsgA sequence has been replaced from CTGCAG to CTGCCG.
star: false
creator: Shoujie Sun
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1361998
component: BBa_K608003
component: BBa_B0015
component: BBa_K1361995
component: BBa_B0034
component: BBa_K206000
sequence annotation: BBa_K206000
sequence annotation: BBa_B0034
sequence annotation: BBa_K1361995
sequence annotation: BBa_B0015
sequence annotation: BBa_K608003
sequence annotation: BBa_K1361998
sequence: https://synbiohub.org/public/igem/BBa_K1361002_sequence/1

BBa_K1361998
type: Component
persistent identity: component2398553
display id: component2398553
version: 1
top level: BBa_K1361002
definition: BBa_K1361998
access: public

BBa_K608003
type: Component
persistent identity: component2398546
display id: component2398546
version: 1
top level: BBa_K1361002
definition: BBa_K608003
access: public

BBa_B0015
type: Component
persistent identity: component2398541
display id: component2398541
version: 1
top level: BBa_K1361002
definition: BBa_B0015
access: public

BBa_K1361995
type: Component
persistent identity: component2398534
display id: component2398534
version: 1
top level: BBa_K1361002
definition: BBa_K1361995
access: public

BBa_B0034
type: Component
persistent identity: component2398532
display id: component2398532
version: 1
top level: BBa_K1361002
definition: BBa_B0034
access: public

BBa_K206000
type: Component
persistent identity: component2398530
display id: component2398530
version: 1
top level: BBa_K1361002
definition: pBAD
access: public

BBa_K206000
type: SequenceAnnotation
persistent identity: annotation2398530
display id: annotation2398530
version: 1
top level: BBa_K1361002
location: https://synbiohub.org/public/igem/BBa_K1361002/annotation2398530/range2398530/1
component: BBa_K206000

https://synbiohub.org/public/igem/BBa_K1361002/annotation2398530/range2398530/1
type: Range
persistent identity: range2398530
display id: range2398530
version: 1
top level: BBa_K1361002
direction: direction/reverse
start: 1
end: 130
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2398532
display id: annotation2398532
version: 1
top level: BBa_K1361002
location: https://synbiohub.org/public/igem/BBa_K1361002/annotation2398532/range2398532/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1361002/annotation2398532/range2398532/1
type: Range
persistent identity: range2398532
display id: range2398532
version: 1
top level: BBa_K1361002
direction: direction/reverse
start: 139
end: 150
orientation: inline

BBa_K1361995
type: SequenceAnnotation
persistent identity: annotation2398534
display id: annotation2398534
version: 1
top level: BBa_K1361002
location: https://synbiohub.org/public/igem/BBa_K1361002/annotation2398534/range2398534/1
component: BBa_K1361995

https://synbiohub.org/public/igem/BBa_K1361002/annotation2398534/range2398534/1
type: Range
persistent identity: range2398534
display id: range2398534
version: 1
top level: BBa_K1361002
direction: direction/reverse
start: 157
end: 555
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2398541
display id: annotation2398541
version: 1
top level: BBa_K1361002
location: https://synbiohub.org/public/igem/BBa_K1361002/annotation2398541/range2398541/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1361002/annotation2398541/range2398541/1
type: Range
persistent identity: range2398541
display id: range2398541
version: 1
top level: BBa_K1361002
direction: direction/reverse
start: 564
end: 692
orientation: inline

BBa_K608003
type: SequenceAnnotation
persistent identity: annotation2398546
display id: annotation2398546
version: 1
top level: BBa_K1361002
location: https://synbiohub.org/public/igem/BBa_K1361002/annotation2398546/range2398546/1
component: BBa_K608003

https://synbiohub.org/public/igem/BBa_K1361002/annotation2398546/range2398546/1
type: Range
persistent identity: range2398546
display id: range2398546
version: 1
top level: BBa_K1361002
direction: direction/reverse
start: 701
end: 756
orientation: inline

BBa_K1361998
type: SequenceAnnotation
persistent identity: annotation2398553
display id: annotation2398553
version: 1
top level: BBa_K1361002
location: https://synbiohub.org/public/igem/BBa_K1361002/annotation2398553/range2398553/1
component: BBa_K1361998

https://synbiohub.org/public/igem/BBa_K1361002/annotation2398553/range2398553/1
type: Range
persistent identity: range2398553
display id: range2398553
version: 1
top level: BBa_K1361002
direction: direction/reverse
start: 763
end: 1609
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_K1361998
type: ComponentDefinition
persistent identity: BBa K1361998
display id: BBa_K1361998
version: 1
was generated by: iGEM to SBOL conversion
description: Curli Fiber assembly associated protein CsgA, CsgC
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:04Z
mutable provenance: PCR on genomic DNA from E coli. DH5alpha strain. And site-directed mutation by overlap PCR primers.
owned by: james
owned by: myers
mutable description: CsgA, CsgC genes are directly PCRed from E coli. DH5alpha genome as a whole. There is a scar sequence between CsgA and CsgC coding region that contains a promoter sequence may constitutively transcript CsgC gene. CsgA protein is the major subunit of Curli Fiber. CsgC may regulate CsgG outer membrane assembly of channel protein CsgG and pore activity through modification of C230 in CsgG.
discontinued: false
dominant: false
group u list: _1737_
m user id: 0
owner id: 16915
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The PstI loci within CsgA sequence has been replaced from CTGCAG to CTGCCG.
star: false
creator: Shoujie Sun
type: DnaRegion
role: partType/Composite
role: SO:0000804
sequence annotation: start
sequence annotation: CsgA
sequence annotation: stop
sequence annotation: CsgC
sequence: https://synbiohub.org/public/igem/BBa_K1361998_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2392513
display id: annotation2392513
version: 1
top level: BBa_K1361998
location: https://synbiohub.org/public/igem/BBa_K1361998/annotation2392513/range2392513/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K1361998/annotation2392513/range2392513/1
type: Range
persistent identity: range2392513
display id: range2392513
version: 1
top level: BBa_K1361998
direction: direction/forward
start: 1
end: 3
orientation: inline

CsgA
type: SequenceAnnotation
persistent identity: annotation2392512
display id: annotation2392512
version: 1
top level: BBa_K1361998
location: https://synbiohub.org/public/igem/BBa_K1361998/annotation2392512/range2392512/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1361998/annotation2392512/range2392512/1
type: Range
persistent identity: range2392512
display id: range2392512
version: 1
top level: BBa_K1361998
direction: direction/forward
start: 1
end: 456
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2392514
display id: annotation2392514
version: 1
top level: BBa_K1361998
location: https://synbiohub.org/public/igem/BBa_K1361998/annotation2392514/range2392514/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K1361998/annotation2392514/range2392514/1
type: Range
persistent identity: range2392514
display id: range2392514
version: 1
top level: BBa_K1361998
direction: direction/forward
start: 454
end: 456
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2392516
display id: annotation2392516
version: 1
top level: BBa_K1361998
location: https://synbiohub.org/public/igem/BBa_K1361998/annotation2392516/range2392516/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K1361998/annotation2392516/range2392516/1
type: Range
persistent identity: range2392516
display id: range2392516
version: 1
top level: BBa_K1361998
direction: direction/forward
start: 515
end: 517
orientation: inline

CsgC
type: SequenceAnnotation
persistent identity: annotation2392515
display id: annotation2392515
version: 1
top level: BBa_K1361998
location: https://synbiohub.org/public/igem/BBa_K1361998/annotation2392515/range2392515/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1361998/annotation2392515/range2392515/1
type: Range
persistent identity: range2392515
display id: range2392515
version: 1
top level: BBa_K1361998
direction: direction/forward
start: 515
end: 847
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2392517
display id: annotation2392517
version: 1
top level: BBa_K1361998
location: https://synbiohub.org/public/igem/BBa_K1361998/annotation2392517/range2392517/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K1361998/annotation2392517/range2392517/1
type: Range
persistent identity: range2392517
display id: range2392517
version: 1
top level: BBa_K1361998
direction: direction/forward
start: 845
end: 847
orientation: inline

BBa_K1361995
type: ComponentDefinition
persistent identity: BBa K1361995
display id: BBa_K1361995
version: 1
was generated by: iGEM to SBOL conversion
description: CsgBtrunc, curli fiber nucleator with a C-terminnal deletion
created: 2014-10-03T11:00:00Z
modified: 2015-05-08T01:10:04Z
mutable provenance: This part is collected by PCR from genome of E coli. DH5alpha.
owned by: james
owned by: myers
mutable description: This part is Curli fiber nucleator subunit protein CsgB with deletion of C-terminal amino acid from 133 to 155 that is lost the ability to attach outer membrane of the cell.
discontinued: false
dominant: false
experience: experience/None
group u list: _1737_
m user id: 0
owner id: 18617
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Deletion is designed referring Hammer, Neal D., Jens C. Schmidt, and Matthew R. Chapman. "The curli nucleator protein, CsgB, contains an amyloidogenic domain that directs CsgA polymerization." Proceedings of the National Academy of Sciences 104.30 (2007): 12494-12499.
star: false
creator: Shoujie Sun
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cds
sequence: https://synbiohub.org/public/igem/BBa_K1361995_sequence/1

cds
type: SequenceAnnotation
persistent identity: annotation2393388
display id: annotation2393388
version: 1
top level: BBa_K1361995
location: https://synbiohub.org/public/igem/BBa_K1361995/annotation2393388/range2393388/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1361995/annotation2393388/range2393388/1
type: Range
persistent identity: range2393388
display id: range2393388
version: 1
top level: BBa_K1361995
direction: direction/forward
start: 1
end: 399
orientation: inline

BBa_K608003
type: ComponentDefinition
persistent identity: BBa K608003
display id: BBa_K608003
version: 1
was generated by: iGEM to SBOL conversion
description: strong Promoter , medium RBS
created: 2011-09-14T11:00:00Z
modified: 2015-05-08T01:12:52Z
mutable provenance: composite
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: strong Promotor , medium RBS
discontinued: false
dominant: false
experience: experience/None
group u list: _780_
m user id: 0
owner id: 9115
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: composite
star: false
creator: Julia M??ller
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_J23104
component: BBa_B0032
sequence annotation: BBa_J23104
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_K608003_sequence/1

BBa_J23104
type: Component
persistent identity: component2128649
display id: component2128649
version: 1
top level: BBa_K608003
definition: BBa_J23104
access: public

BBa_B0032
type: Component
persistent identity: component2128651
display id: component2128651
version: 1
top level: BBa_K608003
definition: BBa_B0032
access: public

BBa_J23104
type: SequenceAnnotation
persistent identity: annotation2128649
display id: annotation2128649
version: 1
top level: BBa_K608003
location: https://synbiohub.org/public/igem/BBa_K608003/annotation2128649/range2128649/1
component: BBa_J23104

https://synbiohub.org/public/igem/BBa_K608003/annotation2128649/range2128649/1
type: Range
persistent identity: range2128649
display id: range2128649
version: 1
top level: BBa_K608003
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2128651
display id: annotation2128651
version: 1
top level: BBa_K608003
location: https://synbiohub.org/public/igem/BBa_K608003/annotation2128651/range2128651/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K608003/annotation2128651/range2128651/1
type: Range
persistent identity: range2128651
display id: range2128651
version: 1
top level: BBa_K608003
direction: direction/forward
start: 44
end: 56
orientation: inline

pBAD
type: ComponentDefinition
persistent identity: BBa K206000
display id: BBa_K206000
version: 1
was generated by: iGEM to SBOL conversion
description: pBAD strong
created: 2009-10-13T11:00:00Z
modified: 2015-05-08T01:11:23Z
mutable provenance: The sequence was obtained by applying all mutations that upregulated AraC binding and subsequent promoter activity listed in reference [1].
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: pBAD is an E.coli promoter that is induced by L-arabinose. K206000 is a mutagenized pBAD promoter that is responsive to lower concentrations of arabinose than wild type (<partinfo>I13453</partinfo>) and, additionally, exhibits a higher maximum of protein expression as measured by coupling to a fluorescent reporter.
discontinued: false
dominant: false
experience: experience/Works
group u list: _307_
m user id: 0
owner id: 4172
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: There were no special design considerations.
star: false
creator: Amelia Hardjasa
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: promoter
sequence annotation: AraI1
sequence annotation: AraI2
sequence: https://synbiohub.org/public/igem/BBa_K206000_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation2049252
display id: annotation2049252
version: 1
top level: pBAD
location: https://synbiohub.org/public/igem/BBa_K206000/annotation2049252/range2049252/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K206000/annotation2049252/range2049252/1
type: Range
persistent identity: range2049252
display id: range2049252
version: 1
top level: pBAD
direction: direction/forward
start: 1
end: 131
orientation: inline

AraI1
type: SequenceAnnotation
persistent identity: annotation2049253
display id: annotation2049253
version: 1
top level: pBAD
location: https://synbiohub.org/public/igem/BBa_K206000/annotation2049253/range2049253/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_K206000/annotation2049253/range2049253/1
type: Range
persistent identity: range2049253
display id: range2049253
version: 1
top level: pBAD
direction: direction/forward
start: 40
end: 57
orientation: inline

AraI2
type: SequenceAnnotation
persistent identity: annotation2049254
display id: annotation2049254
version: 1
top level: pBAD
location: https://synbiohub.org/public/igem/BBa_K206000/annotation2049254/range2049254/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_K206000/annotation2049254/range2049254/1
type: Range
persistent identity: range2049254
display id: range2049254
version: 1
top level: pBAD
direction: direction/forward
start: 61
end: 78
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_J23104
type: ComponentDefinition
persistent identity: BBa J23104
display id: BBa_J23104
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: isolated from library of promoters
owned by: james
owned by: myers
mutable description: replace later
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23104_sequence/1

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

https://synbiohub.org/public/igem/BBa_K1361002_sequence/1
type: Sequence
persistent identity: BBa K1361002 sequence
display id: BBa_K1361002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatgaaaaacaaattgttatttatgatgttaacaatactgggtgcgcctgggattgcagccgcagcaggttatgatttagctaattcagaatataacttcgcggtaaatgaattgagtaagtcttcatttaatcaggcagccataattggtcaagctgggactaataatagtgctcagttacggcagggaggctcaaaacttttggcggttgttgcgcaagaaggtagtagcaaccgggcaaagattgaccagacaggagattataaccttgcatatattgatcaggcgggcagtgccaacgatgccagtatttcgcaaggtgcttatggtaatactgcgatgattatccagaaaggttctggtaataaagcaaatattacacagtatggtactcaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacagctagctcagtcctaggtattgtgctagctactagagtcacacaggaaagtactagatgaaacttttaaaagtagcagcaattgcagcaatcgtattctccggtagcgctctggcaggtgttgttcctcagtacggcggcggcggtaaccacggtggtggcggtaataatagcggcccaaattctgagctgaacatttaccagtacggtggcggtaactctgcacttgctctgcaaactgatgcccgtaactctgacttgactattacccagcatggcggcggtaatggtgcagatgttggtcagggctcagatgacagctcaatcgatctgacccaacgtggcttcggtaacagcgctactcttgatcagtggaacggcaaaaattctgaaatgacggttaaacagttcggtggtggcaacggtgccgcagttgaccagactgcatctaactcctccgtcaacgtgactcaggttggctttggtaacaacgcgaccgctcatcagtactaatacatcatttgtattacagaaacagggcgcaagccctgttttttttcgggagaagaatatgaatacgttattactccttgcggcactttccagtcagataacctttaatacgacccagcaaggggatgtgtataccattattcctgaagtcactcttactcaatcttgtctgtgcagagtacaaatattgtccctgcgcgaaggcagttcagggcaaagtcagacgaagcaagaaaagaccctttcattgcctgctaatcaacccattgctttgacgaagttgagtttaaatatttccccggacgatcgggtgaaaatagttgttactgtttctgatggacagtcacttcatttatcacaacaatggccgccctcttcagaaaagtcttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1361998_sequence/1
type: Sequence
persistent identity: BBa K1361998 sequence
display id: BBa_K1361998_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaacttttaaaagtagcagcaattgcagcaatcgtattctccggtagcgctctggcaggtgttgttcctcagtacggcggcggcggtaaccacggtggtggcggtaataatagcggcccaaattctgagctgaacatttaccagtacggtggcggtaactctgcacttgctctgcaaactgatgcccgtaactctgacttgactattacccagcatggcggcggtaatggtgcagatgttggtcagggctcagatgacagctcaatcgatctgacccaacgtggcttcggtaacagcgctactcttgatcagtggaacggcaaaaattctgaaatgacggttaaacagttcggtggtggcaacggtgccgcagttgaccagactgcatctaactcctccgtcaacgtgactcaggttggctttggtaacaacgcgaccgctcatcagtactaatacatcatttgtattacagaaacagggcgcaagccctgttttttttcgggagaagaatatgaatacgttattactccttgcggcactttccagtcagataacctttaatacgacccagcaaggggatgtgtataccattattcctgaagtcactcttactcaatcttgtctgtgcagagtacaaatattgtccctgcgcgaaggcagttcagggcaaagtcagacgaagcaagaaaagaccctttcattgcctgctaatcaacccattgctttgacgaagttgagtttaaatatttccccggacgatcgggtgaaaatagttgttactgtttctgatggacagtcacttcatttatcacaacaatggccgccctcttcagaaaagtcttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1361995_sequence/1
type: Sequence
persistent identity: BBa K1361995 sequence
display id: BBa_K1361995_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaacaaattgttatttatgatgttaacaatactgggtgcgcctgggattgcagccgcagcaggttatgatttagctaattcagaatataacttcgcggtaaatgaattgagtaagtcttcatttaatcaggcagccataattggtcaagctgggactaataatagtgctcagttacggcagggaggctcaaaacttttggcggttgttgcgcaagaaggtagtagcaaccgggcaaagattgaccagacaggagattataaccttgcatatattgatcaggcgggcagtgccaacgatgccagtatttcgcaaggtgcttatggtaatactgcgatgattatccagaaaggttctggtaataaagcaaatattacacagtatggtactcaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K608003_sequence/1
type: Sequence
persistent identity: BBa K608003 sequence
display id: BBa_K608003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtattgtgctagctactagagtcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K206000_sequence/1
type: Sequence
persistent identity: BBa K206000 sequence
display id: BBa_K206000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23104_sequence/1
type: Sequence
persistent identity: BBa J23104 sequence
display id: BBa_J23104_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtattgtgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z