LOCUS       BBa_K1361003            1547 bp    DNA     linear       22-Dec-2024
DEFINITION  Curli Fiber generator under the control of T7 promoter with a 
            relatively strong expression of CsgA,C
ACCESSION   BBa_K1361003
VERSION     BBa_K1361003.1  
FEATURES             Location/Qualifiers
     misc_feature    21..32
                     /label=B0034
     promoter        1..20
                     /label=T7 promoter
     RBS             24..27
                     /label=RBS
     promoter        1..32
                     /label=BBa_K525998
     misc_feature    39..41
                     /label=start
     misc_feature    492..494
                     /label=stop
     CDS             39..494
                     /label=CsgB
     CDS             39..494
                     /label=BBa_K1361997
     stem_loop       514..557
                     /label=stem_loop
     misc_feature    503..582
                     /label=BBa_B0010
     terminator      503..582
                     /label=BBa_B0010
     stem_loop       598..617
                     /label=T7 TE
     misc_feature    624..624
                     /label=stop
     polyA_site      618..631
                     /label=polya
     misc_feature    591..631
                     /label=BBa_B0012
     terminator      591..631
                     /label=BBa_B0012
     terminator      503..631
                     /label=BBa_B0015
     promoter        640..674
                     /label=BBa_J23100
     misc_feature    687..690
                     /label=conserved
     RBS             683..694
                     /label=BBa_B0034
     misc_feature    640..694
                     /label=BBa_K880005
     CDS             701..1156
                     /label=CsgA
     misc_feature    701..703
                     /label=start
     misc_feature    1154..1156
                     /label=stop
     CDS             1215..1547
                     /label=CsgC
     misc_feature    1215..1217
                     /label=start
     misc_feature    1545..1547
                     /label=stop
     misc_feature    701..1547
                     /label=BBa_K1361998
ORIGIN
        1 taatacgact cactataggg aaagaggaga aatactagat gaaaaacaaa ttgttattta
       61 tgatgttaac aatactgggt gcgcctggga ttgcagccgc agcaggttat gatttagcta
      121 attcagaata taacttcgcg gtaaatgaat tgagtaagtc ttcatttaat caggcagcca
      181 taattggtca agctgggact aataatagtg ctcagttacg gcagggaggc tcaaaacttt
      241 tggcggttgt tgcgcaagaa ggtagtagca accgggcaaa gattgaccag acaggagatt
      301 ataaccttgc atatattgat caggcgggca gtgccaacga tgccagtatt tcgcaaggtg
      361 cttatggtaa tactgcgatg attatccaga aaggttctgg taataaagca aatattacac
      421 agtatggtac tcaaaaaacg gcaattgtag tgcagagaca gtcgcaaatg gctattcgcg
      481 tgacacaacg ttaatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac
      541 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc
      601 tcaccttcgg gtgggccttt ctgcgtttat atactagagt tgacggctag ctcagtccta
      661 ggtacagtgc tagctactag agaaagagga gaaatactag atgaaacttt taaaagtagc
      721 agcaattgca gcaatcgtat tctccggtag cgctctggca ggtgttgttc ctcagtacgg
      781 cggcggcggt aaccacggtg gtggcggtaa taatagcggc ccaaattctg agctgaacat
      841 ttaccagtac ggtggcggta actctgcact tgctctgcaa actgatgccc gtaactctga
      901 cttgactatt acccagcatg gcggcggtaa tggtgcagat gttggtcagg gctcagatga
      961 cagctcaatc gatctgaccc aacgtggctt cggtaacagc gctactcttg atcagtggaa
     1021 cggcaaaaat tctgaaatga cggttaaaca gttcggtggt ggcaacggtg ccgcagttga
     1081 ccagactgca tctaactcct ccgtcaacgt gactcaggtt ggctttggta acaacgcgac
     1141 cgctcatcag tactaataca tcatttgtat tacagaaaca gggcgcaagc cctgtttttt
     1201 ttcgggagaa gaatatgaat acgttattac tccttgcggc actttccagt cagataacct
     1261 ttaatacgac ccagcaaggg gatgtgtata ccattattcc tgaagtcact cttactcaat
     1321 cttgtctgtg cagagtacaa atattgtccc tgcgcgaagg cagttcaggg caaagtcaga
     1381 cgaagcaaga aaagaccctt tcattgcctg ctaatcaacc cattgctttg acgaagttga
     1441 gtttaaatat ttccccggac gatcgggtga aaatagttgt tactgtttct gatggacagt
     1501 cacttcattt atcacaacaa tggccgccct cttcagaaaa gtcttaa
//