BBa_K1361003
type: ComponentDefinition
persistent identity: BBa K1361003
display id: BBa_K1361003
version: 1
was generated by: iGEM to SBOL conversion
description: Curli Fiber generator under the control of T7 promoter with a relatively strong expression of CsgA,C
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:04Z
mutable provenance: CsgB and CsgAC were collected by PCR from genomic DNA of E coli. DH5alpha strain. And site-directed mutation by overlap PCR primers. Others parts come from Biobricks Attribution Kit.
owned by: james
owned by: myers
mutable description: This part contains the major subunit of curli fiber CsgA and its nucleator CsgB, in which CsgA is constitutive expression(a relatively strong promoter compared to BBa_K1361001) whereas CsgB is under the control of T7 promotor. This part cannot functioning alone without expression of CsgEFG genes in the same cell, for they coding the outer membrane channel secrete system for CsgA and CsgB.
discontinued: false
dominant: false
group u list: _1737_
m user id: 0
owner id: 16915
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: The PstI loci within CsgA sequence has been replaced from CTGCAG to CTGCCG.
star: false
creator: Shoujie Sun
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0015
component: BBa_K1361997
component: BBa_K525998
component: BBa_K1361998
component: BBa_K880005
sequence annotation: BBa_K525998
sequence annotation: BBa_K1361997
sequence annotation: BBa_B0015
sequence annotation: BBa_K880005
sequence annotation: BBa_K1361998
sequence: https://synbiohub.org/public/igem/BBa_K1361003_sequence/1

BBa_B0015
type: Component
persistent identity: component2397380
display id: component2397380
version: 1
top level: BBa_K1361003
definition: BBa_B0015
access: public

BBa_K1361997
type: Component
persistent identity: component2397373
display id: component2397373
version: 1
top level: BBa_K1361003
definition: BBa_K1361997
access: public

BBa_K525998
type: Component
persistent identity: component2397369
display id: component2397369
version: 1
top level: BBa_K1361003
definition: pT7
access: public

BBa_K1361998
type: Component
persistent identity: component2397391
display id: component2397391
version: 1
top level: BBa_K1361003
definition: BBa_K1361998
access: public

BBa_K880005
type: Component
persistent identity: component2397384
display id: component2397384
version: 1
top level: BBa_K1361003
definition: BBa_K880005
access: public

BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2397369
display id: annotation2397369
version: 1
top level: BBa_K1361003
location: https://synbiohub.org/public/igem/BBa_K1361003/annotation2397369/range2397369/1
component: BBa_K525998

https://synbiohub.org/public/igem/BBa_K1361003/annotation2397369/range2397369/1
type: Range
persistent identity: range2397369
display id: range2397369
version: 1
top level: BBa_K1361003
direction: direction/reverse
start: 1
end: 32
orientation: inline

BBa_K1361997
type: SequenceAnnotation
persistent identity: annotation2397373
display id: annotation2397373
version: 1
top level: BBa_K1361003
location: https://synbiohub.org/public/igem/BBa_K1361003/annotation2397373/range2397373/1
component: BBa_K1361997

https://synbiohub.org/public/igem/BBa_K1361003/annotation2397373/range2397373/1
type: Range
persistent identity: range2397373
display id: range2397373
version: 1
top level: BBa_K1361003
direction: direction/reverse
start: 39
end: 494
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2397380
display id: annotation2397380
version: 1
top level: BBa_K1361003
location: https://synbiohub.org/public/igem/BBa_K1361003/annotation2397380/range2397380/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1361003/annotation2397380/range2397380/1
type: Range
persistent identity: range2397380
display id: range2397380
version: 1
top level: BBa_K1361003
direction: direction/reverse
start: 503
end: 631
orientation: inline

BBa_K880005
type: SequenceAnnotation
persistent identity: annotation2397384
display id: annotation2397384
version: 1
top level: BBa_K1361003
location: https://synbiohub.org/public/igem/BBa_K1361003/annotation2397384/range2397384/1
component: BBa_K880005

https://synbiohub.org/public/igem/BBa_K1361003/annotation2397384/range2397384/1
type: Range
persistent identity: range2397384
display id: range2397384
version: 1
top level: BBa_K1361003
direction: direction/reverse
start: 640
end: 694
orientation: inline

BBa_K1361998
type: SequenceAnnotation
persistent identity: annotation2397391
display id: annotation2397391
version: 1
top level: BBa_K1361003
location: https://synbiohub.org/public/igem/BBa_K1361003/annotation2397391/range2397391/1
component: BBa_K1361998

https://synbiohub.org/public/igem/BBa_K1361003/annotation2397391/range2397391/1
type: Range
persistent identity: range2397391
display id: range2397391
version: 1
top level: BBa_K1361003
direction: direction/reverse
start: 701
end: 1547
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_K880005
type: ComponentDefinition
persistent identity: BBa K880005
display id: BBa_K880005
version: 1
was generated by: iGEM to SBOL conversion
description: Strong promoter, strong RBS combination for high expression levels of proteins
created: 2012-09-28T11:00:00Z
modified: 2015-05-08T01:13:40Z
mutable provenance: It is a combination of strong promoter (J23100) and RBS (B0034).
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -J23100+B0034 -Strong promoter, strong RBS combination for high expression levels of proteins Consensus constitutive promoter and RBS sequence-produces strongest possible expression.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1142_
m user id: 0
owner id: 9403
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Enter design considerations.
star: false
creator: Josh Atkinson, Mike Ferguson, and Ben Parker
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_B0034
component: BBa_J23100
sequence annotation: BBa_J23100
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K880005_sequence/1

BBa_B0034
type: Component
persistent identity: component2204230
display id: component2204230
version: 1
top level: BBa_K880005
definition: BBa_B0034
access: public

BBa_J23100
type: Component
persistent identity: component2204228
display id: component2204228
version: 1
top level: BBa_K880005
definition: BBa_J23100
access: public

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2204228
display id: annotation2204228
version: 1
top level: BBa_K880005
location: https://synbiohub.org/public/igem/BBa_K880005/annotation2204228/range2204228/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K880005/annotation2204228/range2204228/1
type: Range
persistent identity: range2204228
display id: range2204228
version: 1
top level: BBa_K880005
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2204230
display id: annotation2204230
version: 1
top level: BBa_K880005
location: https://synbiohub.org/public/igem/BBa_K880005/annotation2204230/range2204230/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K880005/annotation2204230/range2204230/1
type: Range
persistent identity: range2204230
display id: range2204230
version: 1
top level: BBa_K880005
direction: direction/reverse
start: 44
end: 55
orientation: inline

BBa_K1361998
type: ComponentDefinition
persistent identity: BBa K1361998
display id: BBa_K1361998
version: 1
was generated by: iGEM to SBOL conversion
description: Curli Fiber assembly associated protein CsgA, CsgC
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:04Z
mutable provenance: PCR on genomic DNA from E coli. DH5alpha strain. And site-directed mutation by overlap PCR primers.
owned by: james
owned by: myers
mutable description: CsgA, CsgC genes are directly PCRed from E coli. DH5alpha genome as a whole. There is a scar sequence between CsgA and CsgC coding region that contains a promoter sequence may constitutively transcript CsgC gene. CsgA protein is the major subunit of Curli Fiber. CsgC may regulate CsgG outer membrane assembly of channel protein CsgG and pore activity through modification of C230 in CsgG.
discontinued: false
dominant: false
group u list: _1737_
m user id: 0
owner id: 16915
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The PstI loci within CsgA sequence has been replaced from CTGCAG to CTGCCG.
star: false
creator: Shoujie Sun
type: DnaRegion
role: partType/Composite
role: SO:0000804
sequence annotation: start
sequence annotation: CsgA
sequence annotation: stop
sequence annotation: CsgC
sequence: https://synbiohub.org/public/igem/BBa_K1361998_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2392513
display id: annotation2392513
version: 1
top level: BBa_K1361998
location: https://synbiohub.org/public/igem/BBa_K1361998/annotation2392513/range2392513/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K1361998/annotation2392513/range2392513/1
type: Range
persistent identity: range2392513
display id: range2392513
version: 1
top level: BBa_K1361998
direction: direction/forward
start: 1
end: 3
orientation: inline

CsgA
type: SequenceAnnotation
persistent identity: annotation2392512
display id: annotation2392512
version: 1
top level: BBa_K1361998
location: https://synbiohub.org/public/igem/BBa_K1361998/annotation2392512/range2392512/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1361998/annotation2392512/range2392512/1
type: Range
persistent identity: range2392512
display id: range2392512
version: 1
top level: BBa_K1361998
direction: direction/forward
start: 1
end: 456
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2392514
display id: annotation2392514
version: 1
top level: BBa_K1361998
location: https://synbiohub.org/public/igem/BBa_K1361998/annotation2392514/range2392514/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K1361998/annotation2392514/range2392514/1
type: Range
persistent identity: range2392514
display id: range2392514
version: 1
top level: BBa_K1361998
direction: direction/forward
start: 454
end: 456
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2392516
display id: annotation2392516
version: 1
top level: BBa_K1361998
location: https://synbiohub.org/public/igem/BBa_K1361998/annotation2392516/range2392516/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K1361998/annotation2392516/range2392516/1
type: Range
persistent identity: range2392516
display id: range2392516
version: 1
top level: BBa_K1361998
direction: direction/forward
start: 515
end: 517
orientation: inline

CsgC
type: SequenceAnnotation
persistent identity: annotation2392515
display id: annotation2392515
version: 1
top level: BBa_K1361998
location: https://synbiohub.org/public/igem/BBa_K1361998/annotation2392515/range2392515/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1361998/annotation2392515/range2392515/1
type: Range
persistent identity: range2392515
display id: range2392515
version: 1
top level: BBa_K1361998
direction: direction/forward
start: 515
end: 847
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2392517
display id: annotation2392517
version: 1
top level: BBa_K1361998
location: https://synbiohub.org/public/igem/BBa_K1361998/annotation2392517/range2392517/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K1361998/annotation2392517/range2392517/1
type: Range
persistent identity: range2392517
display id: range2392517
version: 1
top level: BBa_K1361998
direction: direction/forward
start: 845
end: 847
orientation: inline

BBa_K1361997
type: ComponentDefinition
persistent identity: BBa K1361997
display id: BBa_K1361997
version: 1
was generated by: iGEM to SBOL conversion
description: CsgB, curlin nucleator protein, minor subunit in curli complex
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:04Z
mutable provenance: PCR on genomic DNA from E coli. DH5alpha strain.
owned by: james
owned by: myers
mutable description: CsgB is a secretive protein that attached cells' outer membranes. It plays key role in curli fiber polymerization. CsgB is the nucleator for CsgA aggregation in vivo, that facilitate the transformation of soluble CsgA into polymer fiber.
discontinued: false
dominant: false
group u list: _1737_
m user id: 0
owner id: 16915
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: CsgB can be designed under inductively control while make CsgACEFG genes constitutively express respectly. Using this method could achieve the immediate synthesis of curli fiber after inducer has been added.
star: false
creator: Shoujie Sun
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: CsgB
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K1361997_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2392539
display id: annotation2392539
version: 1
top level: BBa_K1361997
location: https://synbiohub.org/public/igem/BBa_K1361997/annotation2392539/range2392539/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K1361997/annotation2392539/range2392539/1
type: Range
persistent identity: range2392539
display id: range2392539
version: 1
top level: BBa_K1361997
direction: direction/forward
start: 1
end: 3
orientation: inline

CsgB
type: SequenceAnnotation
persistent identity: annotation2392541
display id: annotation2392541
version: 1
top level: BBa_K1361997
location: https://synbiohub.org/public/igem/BBa_K1361997/annotation2392541/range2392541/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1361997/annotation2392541/range2392541/1
type: Range
persistent identity: range2392541
display id: range2392541
version: 1
top level: BBa_K1361997
direction: direction/forward
start: 1
end: 456
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2392540
display id: annotation2392540
version: 1
top level: BBa_K1361997
location: https://synbiohub.org/public/igem/BBa_K1361997/annotation2392540/range2392540/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K1361997/annotation2392540/range2392540/1
type: Range
persistent identity: range2392540
display id: range2392540
version: 1
top level: BBa_K1361997
direction: direction/forward
start: 454
end: 456
orientation: inline

pT7
type: ComponentDefinition
persistent identity: BBa K525998
display id: BBa_K525998
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter T7 and RBS
created: 2011-09-12T11:00:00Z
modified: 2015-05-08T01:12:35Z
mutable provenance: Synthesis
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Promoter T7 and RBS
discontinued: false
dominant: false
experience: experience/Works
group u list: _690_
m user id: 0
owner id: 8543
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Synthesis
star: false
creator: Anna Drong
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: T7 promoter
sequence annotation: B0034
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_K525998_sequence/1

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2129432
display id: annotation2129432
version: 1
top level: pT7
location: https://synbiohub.org/public/igem/BBa_K525998/annotation2129432/range2129432/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K525998/annotation2129432/range2129432/1
type: Range
persistent identity: range2129432
display id: range2129432
version: 1
top level: pT7
direction: direction/forward
start: 1
end: 20
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2127788
display id: annotation2127788
version: 1
top level: pT7
location: https://synbiohub.org/public/igem/BBa_K525998/annotation2127788/range2127788/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K525998/annotation2127788/range2127788/1
type: Range
persistent identity: range2127788
display id: range2127788
version: 1
top level: pT7
direction: direction/forward
start: 21
end: 32
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2129433
display id: annotation2129433
version: 1
top level: pT7
location: https://synbiohub.org/public/igem/BBa_K525998/annotation2129433/range2129433/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K525998/annotation2129433/range2129433/1
type: Range
persistent identity: range2129433
display id: range2129433
version: 1
top level: pT7
direction: direction/forward
start: 24
end: 27
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_J23100
type: ComponentDefinition
persistent identity: BBa J23100
display id: BBa_J23100
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Replace later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23100_sequence/1

https://synbiohub.org/public/igem/BBa_K1361003_sequence/1
type: Sequence
persistent identity: BBa K1361003 sequence
display id: BBa_K1361003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggaaagaggagaaatactagatgaaaaacaaattgttatttatgatgttaacaatactgggtgcgcctgggattgcagccgcagcaggttatgatttagctaattcagaatataacttcgcggtaaatgaattgagtaagtcttcatttaatcaggcagccataattggtcaagctgggactaataatagtgctcagttacggcagggaggctcaaaacttttggcggttgttgcgcaagaaggtagtagcaaccgggcaaagattgaccagacaggagattataaccttgcatatattgatcaggcgggcagtgccaacgatgccagtatttcgcaaggtgcttatggtaatactgcgatgattatccagaaaggttctggtaataaagcaaatattacacagtatggtactcaaaaaacggcaattgtagtgcagagacagtcgcaaatggctattcgcgtgacacaacgttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgaaacttttaaaagtagcagcaattgcagcaatcgtattctccggtagcgctctggcaggtgttgttcctcagtacggcggcggcggtaaccacggtggtggcggtaataatagcggcccaaattctgagctgaacatttaccagtacggtggcggtaactctgcacttgctctgcaaactgatgcccgtaactctgacttgactattacccagcatggcggcggtaatggtgcagatgttggtcagggctcagatgacagctcaatcgatctgacccaacgtggcttcggtaacagcgctactcttgatcagtggaacggcaaaaattctgaaatgacggttaaacagttcggtggtggcaacggtgccgcagttgaccagactgcatctaactcctccgtcaacgtgactcaggttggctttggtaacaacgcgaccgctcatcagtactaatacatcatttgtattacagaaacagggcgcaagccctgttttttttcgggagaagaatatgaatacgttattactccttgcggcactttccagtcagataacctttaatacgacccagcaaggggatgtgtataccattattcctgaagtcactcttactcaatcttgtctgtgcagagtacaaatattgtccctgcgcgaaggcagttcagggcaaagtcagacgaagcaagaaaagaccctttcattgcctgctaatcaacccattgctttgacgaagttgagtttaaatatttccccggacgatcgggtgaaaatagttgttactgtttctgatggacagtcacttcatttatcacaacaatggccgccctcttcagaaaagtcttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K880005_sequence/1
type: Sequence
persistent identity: BBa K880005 sequence
display id: BBa_K880005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1361998_sequence/1
type: Sequence
persistent identity: BBa K1361998 sequence
display id: BBa_K1361998_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaacttttaaaagtagcagcaattgcagcaatcgtattctccggtagcgctctggcaggtgttgttcctcagtacggcggcggcggtaaccacggtggtggcggtaataatagcggcccaaattctgagctgaacatttaccagtacggtggcggtaactctgcacttgctctgcaaactgatgcccgtaactctgacttgactattacccagcatggcggcggtaatggtgcagatgttggtcagggctcagatgacagctcaatcgatctgacccaacgtggcttcggtaacagcgctactcttgatcagtggaacggcaaaaattctgaaatgacggttaaacagttcggtggtggcaacggtgccgcagttgaccagactgcatctaactcctccgtcaacgtgactcaggttggctttggtaacaacgcgaccgctcatcagtactaatacatcatttgtattacagaaacagggcgcaagccctgttttttttcgggagaagaatatgaatacgttattactccttgcggcactttccagtcagataacctttaatacgacccagcaaggggatgtgtataccattattcctgaagtcactcttactcaatcttgtctgtgcagagtacaaatattgtccctgcgcgaaggcagttcagggcaaagtcagacgaagcaagaaaagaccctttcattgcctgctaatcaacccattgctttgacgaagttgagtttaaatatttccccggacgatcgggtgaaaatagttgttactgtttctgatggacagtcacttcatttatcacaacaatggccgccctcttcagaaaagtcttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1361997_sequence/1
type: Sequence
persistent identity: BBa K1361997 sequence
display id: BBa_K1361997_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaacaaattgttatttatgatgttaacaatactgggtgcgcctgggattgcagccgcagcaggttatgatttagctaattcagaatataacttcgcggtaaatgaattgagtaagtcttcatttaatcaggcagccataattggtcaagctgggactaataatagtgctcagttacggcagggaggctcaaaacttttggcggttgttgcgcaagaaggtagtagcaaccgggcaaagattgaccagacaggagattataaccttgcatatattgatcaggcgggcagtgccaacgatgccagtatttcgcaaggtgcttatggtaatactgcgatgattatccagaaaggttctggtaataaagcaaatattacacagtatggtactcaaaaaacggcaattgtagtgcagagacagtcgcaaatggctattcgcgtgacacaacgttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K525998_sequence/1
type: Sequence
persistent identity: BBa K525998 sequence
display id: BBa_K525998_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23100_sequence/1
type: Sequence
persistent identity: BBa J23100 sequence
display id: BBa_J23100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z