LOCUS       BBa_K1361004            1656 bp    DNA     linear       30-Dec-2025
DEFINITION  Curli Fiber generator under the control of Pbad promoter with CsgA 
            modified by His tag
ACCESSION   BBa_K1361004
VERSION     BBa_K1361004.1  
FEATURES             Location/Qualifiers
     promoter        1..131
                     /label=promoter
     misc_feature    40..57
                     /label=AraI1
     misc_feature    61..78
                     /label=AraI2
     promoter        1..130
                     /label=BBa_K206000
     misc_feature    143..146
                     /label=conserved
     RBS             139..150
                     /label=BBa_B0034
     misc_feature    157..159
                     /label=start
     misc_feature    610..612
                     /label=stop
     CDS             157..612
                     /label=CsgB
     CDS             157..612
                     /label=BBa_K1361997
     stem_loop       632..675
                     /label=stem_loop
     misc_feature    621..700
                     /label=BBa_B0010
     terminator      621..700
                     /label=BBa_B0010
     stem_loop       716..735
                     /label=T7 TE
     misc_feature    742..742
                     /label=stop
     polyA_site      736..749
                     /label=polya
     misc_feature    709..749
                     /label=BBa_B0012
     terminator      709..749
                     /label=BBa_B0012
     terminator      621..749
                     /label=BBa_B0015
     promoter        758..792
                     /label=BBa_J23104
     RBS             801..813
                     /label=RBS-3\Weak
     RBS             807..810
                     /label=RBS
     misc_feature    801..813
                     /label=BBa_B0032
     RBS             801..813
                     /label=BBa_B0032
     misc_feature    758..813
                     /label=BBa_K608003
     CDS             820..1317
                     /label=cds
     misc_feature    946..966
                     /label=7XHis
     misc_feature    1294..1314
                     /label=7XHis
     CDS             820..1317
                     /label=BBa_K1361994
     CDS             1324..1656
                     /label=cds
     misc_feature    1324..1326
                     /label=start
     misc_feature    1654..1656
                     /label=stop
     CDS             1324..1656
                     /label=BBa_K1361993
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcaagatag tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagaa agaggagaaa tactagatga aaaacaaatt gttatttatg
      181 atgttaacaa tactgggtgc gcctgggatt gcagccgcag caggttatga tttagctaat
      241 tcagaatata acttcgcggt aaatgaattg agtaagtctt catttaatca ggcagccata
      301 attggtcaag ctgggactaa taatagtgct cagttacggc agggaggctc aaaacttttg
      361 gcggttgttg cgcaagaagg tagtagcaac cgggcaaaga ttgaccagac aggagattat
      421 aaccttgcat atattgatca ggcgggcagt gccaacgatg ccagtatttc gcaaggtgct
      481 tatggtaata ctgcgatgat tatccagaaa ggttctggta ataaagcaaa tattacacag
      541 tatggtactc aaaaaacggc aattgtagtg cagagacagt cgcaaatggc tattcgcgtg
      601 acacaacgtt aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg
      661 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
      721 accttcgggt gggcctttct gcgtttatat actagagttg acagctagct cagtcctagg
      781 tattgtgcta gctactagag tcacacagga aagtactaga tgaaactttt aaaagtagca
      841 gcaattgcag caatcgtatt ctccggtagc gctctggcag gtgttgttcc tcagtacggc
      901 ggcggcggta accacggtgg tggcggtaat aatagcggcc caaatcacca tcaccatcac
      961 caccattctg agctgaacat ttaccagtac ggtggcggta actctgcact tgctctgcaa
     1021 actgatgccc gtaactctga cttgactatt acccagcatg gcggcggtaa tggtgcagat
     1081 gttggtcagg gctcagatga cagctcaatc gatctgaccc aacgtggctt cggtaacagc
     1141 gctactcttg atcagtggaa cggcaaaaat tctgaaatga cggttaaaca gttcggtggt
     1201 ggcaacggtg ccgcagttga ccagactgca tctaactcct ccgtcaacgt gactcaggtt
     1261 ggctttggta acaacgcgac cgctcatcag taccaccatc accatcacca ccattaatac
     1321 tagatgaata cgttattact ccttgcggca ctttccagtc agataacctt taatacgacc
     1381 cagcaagggg atgtgtatac cattattcct gaagtcactc ttactcaatc ttgtctgtgc
     1441 agagtacaaa tattgtccct gcgcgaaggc agttcagggc aaagtcagac gaagcaagaa
     1501 aagacccttt cattgcctgc taatcaaccc attgctttga cgaagttgag tttaaatatt
     1561 tccccggacg atcgggtgaa aatagttgtt actgtttctg atggacagtc acttcattta
     1621 tcacaacaat ggccgccctc ttcagaaaag tcttaa
//