BBa_K1361004
type: ComponentDefinition
persistent identity: BBa K1361004
display id: BBa_K1361004
version: 1
was generated by: iGEM to SBOL conversion
description: Curli Fiber generator under the control of Pbad promoter with CsgA modified by His tag
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:04Z
mutable provenance: CsgB and CsgC were collected by PCR from genomic DNA of E coli. DH5alpha strain. CsgA-his was synthesized by GeneScrip.
owned by: james
owned by: myers
mutable description: This part contains the major subunit of curli fiber CsgA-his and its nucleator CsgB, in which CsgA-his is constitutive expression whereas CsgB is under the control of Pbad promotor. This part is designed to be used for immediate generate of large amount of curli fiber after adding of the inducer, and also can be used for purification of CsgA by Ni-NTA reign, or absorbing Au-NTA-Ni nano partical. This part cannot functioning alone without expression of CsgEFG genes in the same cell, for they coding the outer membrane channel secrete system for CsgA and CsgB.
discontinued: false
dominant: false
experience: experience/None
group u list: _1737_
m user id: 0
owner id: 16915
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: The modifying of CsgA was referring the supplementary of Lu, Michelle Y., et al. "Synthesis and patterning of tunable multiscale materials with engineered cells." (2014).
star: false
creator: Shoujie Sun
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_K1361997
component: BBa_B0034
component: BBa_K206000
component: BBa_K1361993
component: BBa_K1361994
component: BBa_K608003
component: BBa_B0015
sequence annotation: BBa_K206000
sequence annotation: BBa_B0034
sequence annotation: BBa_K1361997
sequence annotation: BBa_B0015
sequence annotation: BBa_K608003
sequence annotation: BBa_K1361994
sequence annotation: BBa_K1361993
sequence: https://synbiohub.org/public/igem/BBa_K1361004_sequence/1

BBa_K1361997
type: Component
persistent identity: component2397411
display id: component2397411
version: 1
top level: BBa_K1361004
definition: BBa_K1361997
access: public

BBa_B0034
type: Component
persistent identity: component2397407
display id: component2397407
version: 1
top level: BBa_K1361004
definition: BBa_B0034
access: public

BBa_K206000
type: Component
persistent identity: component2397405
display id: component2397405
version: 1
top level: BBa_K1361004
definition: pBAD
access: public

BBa_K1361993
type: Component
persistent identity: component2397431
display id: component2397431
version: 1
top level: BBa_K1361004
definition: BBa_K1361993
access: public

BBa_K1361994
type: Component
persistent identity: component2397427
display id: component2397427
version: 1
top level: BBa_K1361004
definition: BBa_K1361994
access: public

BBa_K608003
type: Component
persistent identity: component2397423
display id: component2397423
version: 1
top level: BBa_K1361004
definition: BBa_K608003
access: public

BBa_B0015
type: Component
persistent identity: component2397418
display id: component2397418
version: 1
top level: BBa_K1361004
definition: BBa_B0015
access: public

BBa_K206000
type: SequenceAnnotation
persistent identity: annotation2397405
display id: annotation2397405
version: 1
top level: BBa_K1361004
location: https://synbiohub.org/public/igem/BBa_K1361004/annotation2397405/range2397405/1
component: BBa_K206000

https://synbiohub.org/public/igem/BBa_K1361004/annotation2397405/range2397405/1
type: Range
persistent identity: range2397405
display id: range2397405
version: 1
top level: BBa_K1361004
direction: direction/reverse
start: 1
end: 130
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2397407
display id: annotation2397407
version: 1
top level: BBa_K1361004
location: https://synbiohub.org/public/igem/BBa_K1361004/annotation2397407/range2397407/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1361004/annotation2397407/range2397407/1
type: Range
persistent identity: range2397407
display id: range2397407
version: 1
top level: BBa_K1361004
direction: direction/reverse
start: 139
end: 150
orientation: inline

BBa_K1361997
type: SequenceAnnotation
persistent identity: annotation2397411
display id: annotation2397411
version: 1
top level: BBa_K1361004
location: https://synbiohub.org/public/igem/BBa_K1361004/annotation2397411/range2397411/1
component: BBa_K1361997

https://synbiohub.org/public/igem/BBa_K1361004/annotation2397411/range2397411/1
type: Range
persistent identity: range2397411
display id: range2397411
version: 1
top level: BBa_K1361004
direction: direction/reverse
start: 157
end: 612
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2397418
display id: annotation2397418
version: 1
top level: BBa_K1361004
location: https://synbiohub.org/public/igem/BBa_K1361004/annotation2397418/range2397418/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1361004/annotation2397418/range2397418/1
type: Range
persistent identity: range2397418
display id: range2397418
version: 1
top level: BBa_K1361004
direction: direction/reverse
start: 621
end: 749
orientation: inline

BBa_K608003
type: SequenceAnnotation
persistent identity: annotation2397423
display id: annotation2397423
version: 1
top level: BBa_K1361004
location: https://synbiohub.org/public/igem/BBa_K1361004/annotation2397423/range2397423/1
component: BBa_K608003

https://synbiohub.org/public/igem/BBa_K1361004/annotation2397423/range2397423/1
type: Range
persistent identity: range2397423
display id: range2397423
version: 1
top level: BBa_K1361004
direction: direction/reverse
start: 758
end: 813
orientation: inline

BBa_K1361994
type: SequenceAnnotation
persistent identity: annotation2397427
display id: annotation2397427
version: 1
top level: BBa_K1361004
location: https://synbiohub.org/public/igem/BBa_K1361004/annotation2397427/range2397427/1
component: BBa_K1361994

https://synbiohub.org/public/igem/BBa_K1361004/annotation2397427/range2397427/1
type: Range
persistent identity: range2397427
display id: range2397427
version: 1
top level: BBa_K1361004
direction: direction/reverse
start: 820
end: 1317
orientation: inline

BBa_K1361993
type: SequenceAnnotation
persistent identity: annotation2397431
display id: annotation2397431
version: 1
top level: BBa_K1361004
location: https://synbiohub.org/public/igem/BBa_K1361004/annotation2397431/range2397431/1
component: BBa_K1361993

https://synbiohub.org/public/igem/BBa_K1361004/annotation2397431/range2397431/1
type: Range
persistent identity: range2397431
display id: range2397431
version: 1
top level: BBa_K1361004
direction: direction/reverse
start: 1324
end: 1656
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_K1361997
type: ComponentDefinition
persistent identity: BBa K1361997
display id: BBa_K1361997
version: 1
was generated by: iGEM to SBOL conversion
description: CsgB, curlin nucleator protein, minor subunit in curli complex
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:04Z
mutable provenance: PCR on genomic DNA from E coli. DH5alpha strain.
owned by: james
owned by: myers
mutable description: CsgB is a secretive protein that attached cells' outer membranes. It plays key role in curli fiber polymerization. CsgB is the nucleator for CsgA aggregation in vivo, that facilitate the transformation of soluble CsgA into polymer fiber.
discontinued: false
dominant: false
group u list: _1737_
m user id: 0
owner id: 16915
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: CsgB can be designed under inductively control while make CsgACEFG genes constitutively express respectly. Using this method could achieve the immediate synthesis of curli fiber after inducer has been added.
star: false
creator: Shoujie Sun
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: CsgB
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K1361997_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2392539
display id: annotation2392539
version: 1
top level: BBa_K1361997
location: https://synbiohub.org/public/igem/BBa_K1361997/annotation2392539/range2392539/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K1361997/annotation2392539/range2392539/1
type: Range
persistent identity: range2392539
display id: range2392539
version: 1
top level: BBa_K1361997
direction: direction/forward
start: 1
end: 3
orientation: inline

CsgB
type: SequenceAnnotation
persistent identity: annotation2392541
display id: annotation2392541
version: 1
top level: BBa_K1361997
location: https://synbiohub.org/public/igem/BBa_K1361997/annotation2392541/range2392541/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1361997/annotation2392541/range2392541/1
type: Range
persistent identity: range2392541
display id: range2392541
version: 1
top level: BBa_K1361997
direction: direction/forward
start: 1
end: 456
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2392540
display id: annotation2392540
version: 1
top level: BBa_K1361997
location: https://synbiohub.org/public/igem/BBa_K1361997/annotation2392540/range2392540/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K1361997/annotation2392540/range2392540/1
type: Range
persistent identity: range2392540
display id: range2392540
version: 1
top level: BBa_K1361997
direction: direction/forward
start: 454
end: 456
orientation: inline

BBa_K1361994
type: ComponentDefinition
persistent identity: BBa K1361994
display id: BBa_K1361994
version: 1
was generated by: iGEM to SBOL conversion
description: Curli Fiber major monomer-CsgA with his tag
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:04Z
mutable provenance: CsgA-his was synthesized by GeneScrip.
owned by: james
owned by: myers
mutable description: This part is CsgA(BBa_K1361999) with twin 7Xhis tag. And can be used for purified of CsgA monomer or absorbing Au-NTA-Ni nano partical. CsgA is the major monomer of curli fiber. In vivo, its alone has little polymerizability and CsgB protein is requested for quick polymerization of CsgA.
discontinued: false
dominant: false
group u list: _1737_
m user id: 0
owner id: 16915
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The modifying of CsgA was referring the supplementary of Lu, Michelle Y., et al. "Synthesis and patterning of tunable multiscale materials with engineered cells." (2014).
star: false
creator: Shoujie Sun
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cds
sequence annotation: 7XHis
sequence: https://synbiohub.org/public/igem/BBa_K1361994_sequence/1

cds
type: SequenceAnnotation
persistent identity: annotation2397393
display id: annotation2397393
version: 1
top level: BBa_K1361994
location: https://synbiohub.org/public/igem/BBa_K1361994/annotation2397393/range2397393/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1361994/annotation2397393/range2397393/1
type: Range
persistent identity: range2397393
display id: range2397393
version: 1
top level: BBa_K1361994
direction: direction/forward
start: 1
end: 498
orientation: inline

7XHis
type: SequenceAnnotation
persistent identity: annotation2397394
display id: annotation2397394
version: 1
top level: BBa_K1361994
location: https://synbiohub.org/public/igem/BBa_K1361994/annotation2397394/range2397394/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_K1361994/annotation2397394/range2397394/1
type: Range
persistent identity: range2397394
display id: range2397394
version: 1
top level: BBa_K1361994
direction: direction/forward
start: 127
end: 147
orientation: inline

7XHis
type: SequenceAnnotation
persistent identity: annotation2397395
display id: annotation2397395
version: 1
top level: BBa_K1361994
location: https://synbiohub.org/public/igem/BBa_K1361994/annotation2397395/range2397395/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_K1361994/annotation2397395/range2397395/1
type: Range
persistent identity: range2397395
display id: range2397395
version: 1
top level: BBa_K1361994
direction: direction/forward
start: 475
end: 495
orientation: inline

BBa_K1361993
type: ComponentDefinition
persistent identity: BBa K1361993
display id: BBa_K1361993
version: 1
was generated by: iGEM to SBOL conversion
description: CsgC, curli fiber secrete accessory molecules
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:04Z
mutable provenance: CsgC were collected by PCR from genomic DNA of E coli. DH5alpha strain.
owned by: james
owned by: myers
mutable description: Within the periplasm, CsgC may regulate CsgG outer membrane assembly and pore activity through modification of C230 in CsgG. And its essential for curli fiber assembly.
discontinued: false
dominant: false
group u list: _1737_
m user id: 0
owner id: 16915
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Shoujie Sun
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cds
sequence annotation: start
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K1361993_sequence/1

cds
type: SequenceAnnotation
persistent identity: annotation2397399
display id: annotation2397399
version: 1
top level: BBa_K1361993
location: https://synbiohub.org/public/igem/BBa_K1361993/annotation2397399/range2397399/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1361993/annotation2397399/range2397399/1
type: Range
persistent identity: range2397399
display id: range2397399
version: 1
top level: BBa_K1361993
direction: direction/forward
start: 1
end: 333
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2397400
display id: annotation2397400
version: 1
top level: BBa_K1361993
location: https://synbiohub.org/public/igem/BBa_K1361993/annotation2397400/range2397400/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K1361993/annotation2397400/range2397400/1
type: Range
persistent identity: range2397400
display id: range2397400
version: 1
top level: BBa_K1361993
direction: direction/forward
start: 1
end: 3
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2397401
display id: annotation2397401
version: 1
top level: BBa_K1361993
location: https://synbiohub.org/public/igem/BBa_K1361993/annotation2397401/range2397401/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K1361993/annotation2397401/range2397401/1
type: Range
persistent identity: range2397401
display id: range2397401
version: 1
top level: BBa_K1361993
direction: direction/forward
start: 331
end: 333
orientation: inline

BBa_K608003
type: ComponentDefinition
persistent identity: BBa K608003
display id: BBa_K608003
version: 1
was generated by: iGEM to SBOL conversion
description: strong Promoter , medium RBS
created: 2011-09-14T11:00:00Z
modified: 2015-05-08T01:12:52Z
mutable provenance: composite
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: strong Promotor , medium RBS
discontinued: false
dominant: false
experience: experience/None
group u list: _780_
m user id: 0
owner id: 9115
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: composite
star: false
creator: Julia M??ller
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_J23104
component: BBa_B0032
sequence annotation: BBa_J23104
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_K608003_sequence/1

BBa_J23104
type: Component
persistent identity: component2128649
display id: component2128649
version: 1
top level: BBa_K608003
definition: BBa_J23104
access: public

BBa_B0032
type: Component
persistent identity: component2128651
display id: component2128651
version: 1
top level: BBa_K608003
definition: BBa_B0032
access: public

BBa_J23104
type: SequenceAnnotation
persistent identity: annotation2128649
display id: annotation2128649
version: 1
top level: BBa_K608003
location: https://synbiohub.org/public/igem/BBa_K608003/annotation2128649/range2128649/1
component: BBa_J23104

https://synbiohub.org/public/igem/BBa_K608003/annotation2128649/range2128649/1
type: Range
persistent identity: range2128649
display id: range2128649
version: 1
top level: BBa_K608003
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2128651
display id: annotation2128651
version: 1
top level: BBa_K608003
location: https://synbiohub.org/public/igem/BBa_K608003/annotation2128651/range2128651/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K608003/annotation2128651/range2128651/1
type: Range
persistent identity: range2128651
display id: range2128651
version: 1
top level: BBa_K608003
direction: direction/forward
start: 44
end: 56
orientation: inline

pBAD
type: ComponentDefinition
persistent identity: BBa K206000
display id: BBa_K206000
version: 1
was generated by: iGEM to SBOL conversion
description: pBAD strong
created: 2009-10-13T11:00:00Z
modified: 2015-05-08T01:11:23Z
mutable provenance: The sequence was obtained by applying all mutations that upregulated AraC binding and subsequent promoter activity listed in reference [1].
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: pBAD is an E.coli promoter that is induced by L-arabinose. K206000 is a mutagenized pBAD promoter that is responsive to lower concentrations of arabinose than wild type (<partinfo>I13453</partinfo>) and, additionally, exhibits a higher maximum of protein expression as measured by coupling to a fluorescent reporter.
discontinued: false
dominant: false
experience: experience/Works
group u list: _307_
m user id: 0
owner id: 4172
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: There were no special design considerations.
star: false
creator: Amelia Hardjasa
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: promoter
sequence annotation: AraI1
sequence annotation: AraI2
sequence: https://synbiohub.org/public/igem/BBa_K206000_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation2049252
display id: annotation2049252
version: 1
top level: pBAD
location: https://synbiohub.org/public/igem/BBa_K206000/annotation2049252/range2049252/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K206000/annotation2049252/range2049252/1
type: Range
persistent identity: range2049252
display id: range2049252
version: 1
top level: pBAD
direction: direction/forward
start: 1
end: 131
orientation: inline

AraI1
type: SequenceAnnotation
persistent identity: annotation2049253
display id: annotation2049253
version: 1
top level: pBAD
location: https://synbiohub.org/public/igem/BBa_K206000/annotation2049253/range2049253/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_K206000/annotation2049253/range2049253/1
type: Range
persistent identity: range2049253
display id: range2049253
version: 1
top level: pBAD
direction: direction/forward
start: 40
end: 57
orientation: inline

AraI2
type: SequenceAnnotation
persistent identity: annotation2049254
display id: annotation2049254
version: 1
top level: pBAD
location: https://synbiohub.org/public/igem/BBa_K206000/annotation2049254/range2049254/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_K206000/annotation2049254/range2049254/1
type: Range
persistent identity: range2049254
display id: range2049254
version: 1
top level: pBAD
direction: direction/forward
start: 61
end: 78
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_J23104
type: ComponentDefinition
persistent identity: BBa J23104
display id: BBa_J23104
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: isolated from library of promoters
owned by: james
owned by: myers
mutable description: replace later
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23104_sequence/1

https://synbiohub.org/public/igem/BBa_K1361004_sequence/1
type: Sequence
persistent identity: BBa K1361004 sequence
display id: BBa_K1361004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatgaaaaacaaattgttatttatgatgttaacaatactgggtgcgcctgggattgcagccgcagcaggttatgatttagctaattcagaatataacttcgcggtaaatgaattgagtaagtcttcatttaatcaggcagccataattggtcaagctgggactaataatagtgctcagttacggcagggaggctcaaaacttttggcggttgttgcgcaagaaggtagtagcaaccgggcaaagattgaccagacaggagattataaccttgcatatattgatcaggcgggcagtgccaacgatgccagtatttcgcaaggtgcttatggtaatactgcgatgattatccagaaaggttctggtaataaagcaaatattacacagtatggtactcaaaaaacggcaattgtagtgcagagacagtcgcaaatggctattcgcgtgacacaacgttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacagctagctcagtcctaggtattgtgctagctactagagtcacacaggaaagtactagatgaaacttttaaaagtagcagcaattgcagcaatcgtattctccggtagcgctctggcaggtgttgttcctcagtacggcggcggcggtaaccacggtggtggcggtaataatagcggcccaaatcaccatcaccatcaccaccattctgagctgaacatttaccagtacggtggcggtaactctgcacttgctctgcaaactgatgcccgtaactctgacttgactattacccagcatggcggcggtaatggtgcagatgttggtcagggctcagatgacagctcaatcgatctgacccaacgtggcttcggtaacagcgctactcttgatcagtggaacggcaaaaattctgaaatgacggttaaacagttcggtggtggcaacggtgccgcagttgaccagactgcatctaactcctccgtcaacgtgactcaggttggctttggtaacaacgcgaccgctcatcagtaccaccatcaccatcaccaccattaatactagatgaatacgttattactccttgcggcactttccagtcagataacctttaatacgacccagcaaggggatgtgtataccattattcctgaagtcactcttactcaatcttgtctgtgcagagtacaaatattgtccctgcgcgaaggcagttcagggcaaagtcagacgaagcaagaaaagaccctttcattgcctgctaatcaacccattgctttgacgaagttgagtttaaatatttccccggacgatcgggtgaaaatagttgttactgtttctgatggacagtcacttcatttatcacaacaatggccgccctcttcagaaaagtcttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1361997_sequence/1
type: Sequence
persistent identity: BBa K1361997 sequence
display id: BBa_K1361997_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaacaaattgttatttatgatgttaacaatactgggtgcgcctgggattgcagccgcagcaggttatgatttagctaattcagaatataacttcgcggtaaatgaattgagtaagtcttcatttaatcaggcagccataattggtcaagctgggactaataatagtgctcagttacggcagggaggctcaaaacttttggcggttgttgcgcaagaaggtagtagcaaccgggcaaagattgaccagacaggagattataaccttgcatatattgatcaggcgggcagtgccaacgatgccagtatttcgcaaggtgcttatggtaatactgcgatgattatccagaaaggttctggtaataaagcaaatattacacagtatggtactcaaaaaacggcaattgtagtgcagagacagtcgcaaatggctattcgcgtgacacaacgttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1361994_sequence/1
type: Sequence
persistent identity: BBa K1361994 sequence
display id: BBa_K1361994_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaacttttaaaagtagcagcaattgcagcaatcgtattctccggtagcgctctggcaggtgttgttcctcagtacggcggcggcggtaaccacggtggtggcggtaataatagcggcccaaatcaccatcaccatcaccaccattctgagctgaacatttaccagtacggtggcggtaactctgcacttgctctgcaaactgatgcccgtaactctgacttgactattacccagcatggcggcggtaatggtgcagatgttggtcagggctcagatgacagctcaatcgatctgacccaacgtggcttcggtaacagcgctactcttgatcagtggaacggcaaaaattctgaaatgacggttaaacagttcggtggtggcaacggtgccgcagttgaccagactgcatctaactcctccgtcaacgtgactcaggttggctttggtaacaacgcgaccgctcatcagtaccaccatcaccatcaccaccattaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1361993_sequence/1
type: Sequence
persistent identity: BBa K1361993 sequence
display id: BBa_K1361993_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaatacgttattactccttgcggcactttccagtcagataacctttaatacgacccagcaaggggatgtgtataccattattcctgaagtcactcttactcaatcttgtctgtgcagagtacaaatattgtccctgcgcgaaggcagttcagggcaaagtcagacgaagcaagaaaagaccctttcattgcctgctaatcaacccattgctttgacgaagttgagtttaaatatttccccggacgatcgggtgaaaatagttgttactgtttctgatggacagtcacttcatttatcacaacaatggccgccctcttcagaaaagtcttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K608003_sequence/1
type: Sequence
persistent identity: BBa K608003 sequence
display id: BBa_K608003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtattgtgctagctactagagtcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K206000_sequence/1
type: Sequence
persistent identity: BBa K206000 sequence
display id: BBa_K206000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23104_sequence/1
type: Sequence
persistent identity: BBa J23104 sequence
display id: BBa_J23104_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtattgtgctagc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z