LOCUS       BBa_K1361006            1608 bp    DNA     linear       23-Dec-2024
DEFINITION  Curli Fiber generator where CsgBtrunc, a dissociative nucleator, 
            under the control of Pbad promoter
ACCESSION   BBa_K1361006
VERSION     BBa_K1361006.1  
FEATURES             Location/Qualifiers
     promoter        1..131
                     /label=promoter
     misc_feature    40..57
                     /label=AraI1
     misc_feature    61..78
                     /label=AraI2
     promoter        1..130
                     /label=BBa_K206000
     misc_feature    143..146
                     /label=conserved
     RBS             139..150
                     /label=BBa_B0034
     CDS             157..555
                     /label=cds
     CDS             157..555
                     /label=BBa_K1361995
     stem_loop       575..618
                     /label=stem_loop
     misc_feature    564..643
                     /label=BBa_B0010
     terminator      564..643
                     /label=BBa_B0010
     stem_loop       659..678
                     /label=T7 TE
     misc_feature    685..685
                     /label=stop
     polyA_site      679..692
                     /label=polya
     misc_feature    652..692
                     /label=BBa_B0012
     terminator      652..692
                     /label=BBa_B0012
     terminator      564..692
                     /label=BBa_B0015
     promoter        701..735
                     /label=BBa_J23100
     misc_feature    748..751
                     /label=conserved
     RBS             744..755
                     /label=BBa_B0034
     misc_feature    701..755
                     /label=BBa_K880005
     CDS             762..1217
                     /label=CsgA
     misc_feature    762..764
                     /label=start
     misc_feature    1215..1217
                     /label=stop
     CDS             1276..1608
                     /label=CsgC
     misc_feature    1276..1278
                     /label=start
     misc_feature    1606..1608
                     /label=stop
     misc_feature    762..1608
                     /label=BBa_K1361998
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcaagatag tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagaa agaggagaaa tactagatga aaaacaaatt gttatttatg
      181 atgttaacaa tactgggtgc gcctgggatt gcagccgcag caggttatga tttagctaat
      241 tcagaatata acttcgcggt aaatgaattg agtaagtctt catttaatca ggcagccata
      301 attggtcaag ctgggactaa taatagtgct cagttacggc agggaggctc aaaacttttg
      361 gcggttgttg cgcaagaagg tagtagcaac cgggcaaaga ttgaccagac aggagattat
      421 aaccttgcat atattgatca ggcgggcagt gccaacgatg ccagtatttc gcaaggtgct
      481 tatggtaata ctgcgatgat tatccagaaa ggttctggta ataaagcaaa tattacacag
      541 tatggtactc aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
      601 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
      661 ctcaccttcg ggtgggcctt tctgcgttta tatactagag ttgacggcta gctcagtcct
      721 aggtacagtg ctagctacta gagaaagagg agaaatacta gatgaaactt ttaaaagtag
      781 cagcaattgc agcaatcgta ttctccggta gcgctctggc aggtgttgtt cctcagtacg
      841 gcggcggcgg taaccacggt ggtggcggta ataatagcgg cccaaattct gagctgaaca
      901 tttaccagta cggtggcggt aactctgcac ttgctctgca aactgatgcc cgtaactctg
      961 acttgactat tacccagcat ggcggcggta atggtgcaga tgttggtcag ggctcagatg
     1021 acagctcaat cgatctgacc caacgtggct tcggtaacag cgctactctt gatcagtgga
     1081 acggcaaaaa ttctgaaatg acggttaaac agttcggtgg tggcaacggt gccgcagttg
     1141 accagactgc atctaactcc tccgtcaacg tgactcaggt tggctttggt aacaacgcga
     1201 ccgctcatca gtactaatac atcatttgta ttacagaaac agggcgcaag ccctgttttt
     1261 tttcgggaga agaatatgaa tacgttatta ctccttgcgg cactttccag tcagataacc
     1321 tttaatacga cccagcaagg ggatgtgtat accattattc ctgaagtcac tcttactcaa
     1381 tcttgtctgt gcagagtaca aatattgtcc ctgcgcgaag gcagttcagg gcaaagtcag
     1441 acgaagcaag aaaagaccct ttcattgcct gctaatcaac ccattgcttt gacgaagttg
     1501 agtttaaata tttccccgga cgatcgggtg aaaatagttg ttactgtttc tgatggacag
     1561 tcacttcatt tatcacaaca atggccgccc tcttcagaaa agtcttaa
//