BBa_K1361006
type: ComponentDefinition
persistent identity: BBa K1361006
display id: BBa_K1361006
version: 1
was generated by: iGEM to SBOL conversion
description: Curli Fiber generator where CsgBtrunc, a dissociative nucleator, under the control of Pbad promoter
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:04Z
mutable provenance: CsgAC and CsgBtrunc were collected by PCR from genome of E coli DH5alpha respectively.
owned by: james
owned by: myers
mutable description: This part contains the major subunit of curli fiber CsgA and its nucleator CsgBtrunc, in which CsgA is constitutive expression whereas CsgBtrunc is under the control of Pbad promotor. CsgBtrunc is different from native CsgB from the deletion of C-terminal amino acid from 133 to 155 that it cannot attach cell outer membrane. And CsgBtrunc itself is highly aggregative in the culture. This part is designed to be used for immediate generate of large amount of curli fiber after adding of the inducer and a stronger promoter for CsgA was chosen to parallel with BBa_K1361002. This part cannot functioning alone without expression of CsgEFG genes in the same cell, for they coding the outer membrane channel secrete system for CsgA and CsgB.
discontinued: false
dominant: false
group u list: _1737_
m user id: 0
owner id: 16915
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: The PstI loci within CsgA sequence has been replaced from CTGCAG to CTGCCG.
star: false
creator: Shoujie Sun
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_K206000
component: BBa_K1361995
component: BBa_B0034
component: BBa_K880005
component: BBa_B0015
component: BBa_K1361998
sequence annotation: BBa_K206000
sequence annotation: BBa_B0034
sequence annotation: BBa_K1361995
sequence annotation: BBa_B0015
sequence annotation: BBa_K880005
sequence annotation: BBa_K1361998
sequence: https://synbiohub.org/public/igem/BBa_K1361006_sequence/1

BBa_K206000
type: Component
persistent identity: component2398558
display id: component2398558
version: 1
top level: BBa_K1361006
definition: pBAD
access: public

BBa_K1361995
type: Component
persistent identity: component2398562
display id: component2398562
version: 1
top level: BBa_K1361006
definition: BBa_K1361995
access: public

BBa_B0034
type: Component
persistent identity: component2398560
display id: component2398560
version: 1
top level: BBa_K1361006
definition: BBa_B0034
access: public

BBa_K880005
type: Component
persistent identity: component2398573
display id: component2398573
version: 1
top level: BBa_K1361006
definition: BBa_K880005
access: public

BBa_B0015
type: Component
persistent identity: component2398569
display id: component2398569
version: 1
top level: BBa_K1361006
definition: BBa_B0015
access: public

BBa_K1361998
type: Component
persistent identity: component2398580
display id: component2398580
version: 1
top level: BBa_K1361006
definition: BBa_K1361998
access: public

BBa_K206000
type: SequenceAnnotation
persistent identity: annotation2398558
display id: annotation2398558
version: 1
top level: BBa_K1361006
location: https://synbiohub.org/public/igem/BBa_K1361006/annotation2398558/range2398558/1
component: BBa_K206000

https://synbiohub.org/public/igem/BBa_K1361006/annotation2398558/range2398558/1
type: Range
persistent identity: range2398558
display id: range2398558
version: 1
top level: BBa_K1361006
direction: direction/reverse
start: 1
end: 130
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2398560
display id: annotation2398560
version: 1
top level: BBa_K1361006
location: https://synbiohub.org/public/igem/BBa_K1361006/annotation2398560/range2398560/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1361006/annotation2398560/range2398560/1
type: Range
persistent identity: range2398560
display id: range2398560
version: 1
top level: BBa_K1361006
direction: direction/reverse
start: 139
end: 150
orientation: inline

BBa_K1361995
type: SequenceAnnotation
persistent identity: annotation2398562
display id: annotation2398562
version: 1
top level: BBa_K1361006
location: https://synbiohub.org/public/igem/BBa_K1361006/annotation2398562/range2398562/1
component: BBa_K1361995

https://synbiohub.org/public/igem/BBa_K1361006/annotation2398562/range2398562/1
type: Range
persistent identity: range2398562
display id: range2398562
version: 1
top level: BBa_K1361006
direction: direction/reverse
start: 157
end: 555
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2398569
display id: annotation2398569
version: 1
top level: BBa_K1361006
location: https://synbiohub.org/public/igem/BBa_K1361006/annotation2398569/range2398569/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1361006/annotation2398569/range2398569/1
type: Range
persistent identity: range2398569
display id: range2398569
version: 1
top level: BBa_K1361006
direction: direction/reverse
start: 564
end: 692
orientation: inline

BBa_K880005
type: SequenceAnnotation
persistent identity: annotation2398573
display id: annotation2398573
version: 1
top level: BBa_K1361006
location: https://synbiohub.org/public/igem/BBa_K1361006/annotation2398573/range2398573/1
component: BBa_K880005

https://synbiohub.org/public/igem/BBa_K1361006/annotation2398573/range2398573/1
type: Range
persistent identity: range2398573
display id: range2398573
version: 1
top level: BBa_K1361006
direction: direction/reverse
start: 701
end: 755
orientation: inline

BBa_K1361998
type: SequenceAnnotation
persistent identity: annotation2398580
display id: annotation2398580
version: 1
top level: BBa_K1361006
location: https://synbiohub.org/public/igem/BBa_K1361006/annotation2398580/range2398580/1
component: BBa_K1361998

https://synbiohub.org/public/igem/BBa_K1361006/annotation2398580/range2398580/1
type: Range
persistent identity: range2398580
display id: range2398580
version: 1
top level: BBa_K1361006
direction: direction/reverse
start: 762
end: 1608
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_K880005
type: ComponentDefinition
persistent identity: BBa K880005
display id: BBa_K880005
version: 1
was generated by: iGEM to SBOL conversion
description: Strong promoter, strong RBS combination for high expression levels of proteins
created: 2012-09-28T11:00:00Z
modified: 2015-05-08T01:13:40Z
mutable provenance: It is a combination of strong promoter (J23100) and RBS (B0034).
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -J23100+B0034 -Strong promoter, strong RBS combination for high expression levels of proteins Consensus constitutive promoter and RBS sequence-produces strongest possible expression.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1142_
m user id: 0
owner id: 9403
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Enter design considerations.
star: false
creator: Josh Atkinson, Mike Ferguson, and Ben Parker
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_B0034
component: BBa_J23100
sequence annotation: BBa_J23100
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K880005_sequence/1

BBa_B0034
type: Component
persistent identity: component2204230
display id: component2204230
version: 1
top level: BBa_K880005
definition: BBa_B0034
access: public

BBa_J23100
type: Component
persistent identity: component2204228
display id: component2204228
version: 1
top level: BBa_K880005
definition: BBa_J23100
access: public

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2204228
display id: annotation2204228
version: 1
top level: BBa_K880005
location: https://synbiohub.org/public/igem/BBa_K880005/annotation2204228/range2204228/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K880005/annotation2204228/range2204228/1
type: Range
persistent identity: range2204228
display id: range2204228
version: 1
top level: BBa_K880005
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2204230
display id: annotation2204230
version: 1
top level: BBa_K880005
location: https://synbiohub.org/public/igem/BBa_K880005/annotation2204230/range2204230/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K880005/annotation2204230/range2204230/1
type: Range
persistent identity: range2204230
display id: range2204230
version: 1
top level: BBa_K880005
direction: direction/reverse
start: 44
end: 55
orientation: inline

BBa_K1361998
type: ComponentDefinition
persistent identity: BBa K1361998
display id: BBa_K1361998
version: 1
was generated by: iGEM to SBOL conversion
description: Curli Fiber assembly associated protein CsgA, CsgC
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:04Z
mutable provenance: PCR on genomic DNA from E coli. DH5alpha strain. And site-directed mutation by overlap PCR primers.
owned by: james
owned by: myers
mutable description: CsgA, CsgC genes are directly PCRed from E coli. DH5alpha genome as a whole. There is a scar sequence between CsgA and CsgC coding region that contains a promoter sequence may constitutively transcript CsgC gene. CsgA protein is the major subunit of Curli Fiber. CsgC may regulate CsgG outer membrane assembly of channel protein CsgG and pore activity through modification of C230 in CsgG.
discontinued: false
dominant: false
group u list: _1737_
m user id: 0
owner id: 16915
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The PstI loci within CsgA sequence has been replaced from CTGCAG to CTGCCG.
star: false
creator: Shoujie Sun
type: DnaRegion
role: partType/Composite
role: SO:0000804
sequence annotation: start
sequence annotation: CsgA
sequence annotation: stop
sequence annotation: CsgC
sequence: https://synbiohub.org/public/igem/BBa_K1361998_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2392513
display id: annotation2392513
version: 1
top level: BBa_K1361998
location: https://synbiohub.org/public/igem/BBa_K1361998/annotation2392513/range2392513/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K1361998/annotation2392513/range2392513/1
type: Range
persistent identity: range2392513
display id: range2392513
version: 1
top level: BBa_K1361998
direction: direction/forward
start: 1
end: 3
orientation: inline

CsgA
type: SequenceAnnotation
persistent identity: annotation2392512
display id: annotation2392512
version: 1
top level: BBa_K1361998
location: https://synbiohub.org/public/igem/BBa_K1361998/annotation2392512/range2392512/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1361998/annotation2392512/range2392512/1
type: Range
persistent identity: range2392512
display id: range2392512
version: 1
top level: BBa_K1361998
direction: direction/forward
start: 1
end: 456
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2392514
display id: annotation2392514
version: 1
top level: BBa_K1361998
location: https://synbiohub.org/public/igem/BBa_K1361998/annotation2392514/range2392514/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K1361998/annotation2392514/range2392514/1
type: Range
persistent identity: range2392514
display id: range2392514
version: 1
top level: BBa_K1361998
direction: direction/forward
start: 454
end: 456
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2392516
display id: annotation2392516
version: 1
top level: BBa_K1361998
location: https://synbiohub.org/public/igem/BBa_K1361998/annotation2392516/range2392516/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K1361998/annotation2392516/range2392516/1
type: Range
persistent identity: range2392516
display id: range2392516
version: 1
top level: BBa_K1361998
direction: direction/forward
start: 515
end: 517
orientation: inline

CsgC
type: SequenceAnnotation
persistent identity: annotation2392515
display id: annotation2392515
version: 1
top level: BBa_K1361998
location: https://synbiohub.org/public/igem/BBa_K1361998/annotation2392515/range2392515/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1361998/annotation2392515/range2392515/1
type: Range
persistent identity: range2392515
display id: range2392515
version: 1
top level: BBa_K1361998
direction: direction/forward
start: 515
end: 847
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2392517
display id: annotation2392517
version: 1
top level: BBa_K1361998
location: https://synbiohub.org/public/igem/BBa_K1361998/annotation2392517/range2392517/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K1361998/annotation2392517/range2392517/1
type: Range
persistent identity: range2392517
display id: range2392517
version: 1
top level: BBa_K1361998
direction: direction/forward
start: 845
end: 847
orientation: inline

BBa_K1361995
type: ComponentDefinition
persistent identity: BBa K1361995
display id: BBa_K1361995
version: 1
was generated by: iGEM to SBOL conversion
description: CsgBtrunc, curli fiber nucleator with a C-terminnal deletion
created: 2014-10-03T11:00:00Z
modified: 2015-05-08T01:10:04Z
mutable provenance: This part is collected by PCR from genome of E coli. DH5alpha.
owned by: james
owned by: myers
mutable description: This part is Curli fiber nucleator subunit protein CsgB with deletion of C-terminal amino acid from 133 to 155 that is lost the ability to attach outer membrane of the cell.
discontinued: false
dominant: false
experience: experience/None
group u list: _1737_
m user id: 0
owner id: 18617
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Deletion is designed referring Hammer, Neal D., Jens C. Schmidt, and Matthew R. Chapman. "The curli nucleator protein, CsgB, contains an amyloidogenic domain that directs CsgA polymerization." Proceedings of the National Academy of Sciences 104.30 (2007): 12494-12499.
star: false
creator: Shoujie Sun
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cds
sequence: https://synbiohub.org/public/igem/BBa_K1361995_sequence/1

cds
type: SequenceAnnotation
persistent identity: annotation2393388
display id: annotation2393388
version: 1
top level: BBa_K1361995
location: https://synbiohub.org/public/igem/BBa_K1361995/annotation2393388/range2393388/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1361995/annotation2393388/range2393388/1
type: Range
persistent identity: range2393388
display id: range2393388
version: 1
top level: BBa_K1361995
direction: direction/forward
start: 1
end: 399
orientation: inline

pBAD
type: ComponentDefinition
persistent identity: BBa K206000
display id: BBa_K206000
version: 1
was generated by: iGEM to SBOL conversion
description: pBAD strong
created: 2009-10-13T11:00:00Z
modified: 2015-05-08T01:11:23Z
mutable provenance: The sequence was obtained by applying all mutations that upregulated AraC binding and subsequent promoter activity listed in reference [1].
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: pBAD is an E.coli promoter that is induced by L-arabinose. K206000 is a mutagenized pBAD promoter that is responsive to lower concentrations of arabinose than wild type (<partinfo>I13453</partinfo>) and, additionally, exhibits a higher maximum of protein expression as measured by coupling to a fluorescent reporter.
discontinued: false
dominant: false
experience: experience/Works
group u list: _307_
m user id: 0
owner id: 4172
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: There were no special design considerations.
star: false
creator: Amelia Hardjasa
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: promoter
sequence annotation: AraI1
sequence annotation: AraI2
sequence: https://synbiohub.org/public/igem/BBa_K206000_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation2049252
display id: annotation2049252
version: 1
top level: pBAD
location: https://synbiohub.org/public/igem/BBa_K206000/annotation2049252/range2049252/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K206000/annotation2049252/range2049252/1
type: Range
persistent identity: range2049252
display id: range2049252
version: 1
top level: pBAD
direction: direction/forward
start: 1
end: 131
orientation: inline

AraI1
type: SequenceAnnotation
persistent identity: annotation2049253
display id: annotation2049253
version: 1
top level: pBAD
location: https://synbiohub.org/public/igem/BBa_K206000/annotation2049253/range2049253/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_K206000/annotation2049253/range2049253/1
type: Range
persistent identity: range2049253
display id: range2049253
version: 1
top level: pBAD
direction: direction/forward
start: 40
end: 57
orientation: inline

AraI2
type: SequenceAnnotation
persistent identity: annotation2049254
display id: annotation2049254
version: 1
top level: pBAD
location: https://synbiohub.org/public/igem/BBa_K206000/annotation2049254/range2049254/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_K206000/annotation2049254/range2049254/1
type: Range
persistent identity: range2049254
display id: range2049254
version: 1
top level: pBAD
direction: direction/forward
start: 61
end: 78
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_J23100
type: ComponentDefinition
persistent identity: BBa J23100
display id: BBa_J23100
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Replace later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23100_sequence/1

https://synbiohub.org/public/igem/BBa_K1361006_sequence/1
type: Sequence
persistent identity: BBa K1361006 sequence
display id: BBa_K1361006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatgaaaaacaaattgttatttatgatgttaacaatactgggtgcgcctgggattgcagccgcagcaggttatgatttagctaattcagaatataacttcgcggtaaatgaattgagtaagtcttcatttaatcaggcagccataattggtcaagctgggactaataatagtgctcagttacggcagggaggctcaaaacttttggcggttgttgcgcaagaaggtagtagcaaccgggcaaagattgaccagacaggagattataaccttgcatatattgatcaggcgggcagtgccaacgatgccagtatttcgcaaggtgcttatggtaatactgcgatgattatccagaaaggttctggtaataaagcaaatattacacagtatggtactcaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgaaacttttaaaagtagcagcaattgcagcaatcgtattctccggtagcgctctggcaggtgttgttcctcagtacggcggcggcggtaaccacggtggtggcggtaataatagcggcccaaattctgagctgaacatttaccagtacggtggcggtaactctgcacttgctctgcaaactgatgcccgtaactctgacttgactattacccagcatggcggcggtaatggtgcagatgttggtcagggctcagatgacagctcaatcgatctgacccaacgtggcttcggtaacagcgctactcttgatcagtggaacggcaaaaattctgaaatgacggttaaacagttcggtggtggcaacggtgccgcagttgaccagactgcatctaactcctccgtcaacgtgactcaggttggctttggtaacaacgcgaccgctcatcagtactaatacatcatttgtattacagaaacagggcgcaagccctgttttttttcgggagaagaatatgaatacgttattactccttgcggcactttccagtcagataacctttaatacgacccagcaaggggatgtgtataccattattcctgaagtcactcttactcaatcttgtctgtgcagagtacaaatattgtccctgcgcgaaggcagttcagggcaaagtcagacgaagcaagaaaagaccctttcattgcctgctaatcaacccattgctttgacgaagttgagtttaaatatttccccggacgatcgggtgaaaatagttgttactgtttctgatggacagtcacttcatttatcacaacaatggccgccctcttcagaaaagtcttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K880005_sequence/1
type: Sequence
persistent identity: BBa K880005 sequence
display id: BBa_K880005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1361998_sequence/1
type: Sequence
persistent identity: BBa K1361998 sequence
display id: BBa_K1361998_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaacttttaaaagtagcagcaattgcagcaatcgtattctccggtagcgctctggcaggtgttgttcctcagtacggcggcggcggtaaccacggtggtggcggtaataatagcggcccaaattctgagctgaacatttaccagtacggtggcggtaactctgcacttgctctgcaaactgatgcccgtaactctgacttgactattacccagcatggcggcggtaatggtgcagatgttggtcagggctcagatgacagctcaatcgatctgacccaacgtggcttcggtaacagcgctactcttgatcagtggaacggcaaaaattctgaaatgacggttaaacagttcggtggtggcaacggtgccgcagttgaccagactgcatctaactcctccgtcaacgtgactcaggttggctttggtaacaacgcgaccgctcatcagtactaatacatcatttgtattacagaaacagggcgcaagccctgttttttttcgggagaagaatatgaatacgttattactccttgcggcactttccagtcagataacctttaatacgacccagcaaggggatgtgtataccattattcctgaagtcactcttactcaatcttgtctgtgcagagtacaaatattgtccctgcgcgaaggcagttcagggcaaagtcagacgaagcaagaaaagaccctttcattgcctgctaatcaacccattgctttgacgaagttgagtttaaatatttccccggacgatcgggtgaaaatagttgttactgtttctgatggacagtcacttcatttatcacaacaatggccgccctcttcagaaaagtcttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1361995_sequence/1
type: Sequence
persistent identity: BBa K1361995 sequence
display id: BBa_K1361995_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaacaaattgttatttatgatgttaacaatactgggtgcgcctgggattgcagccgcagcaggttatgatttagctaattcagaatataacttcgcggtaaatgaattgagtaagtcttcatttaatcaggcagccataattggtcaagctgggactaataatagtgctcagttacggcagggaggctcaaaacttttggcggttgttgcgcaagaaggtagtagcaaccgggcaaagattgaccagacaggagattataaccttgcatatattgatcaggcgggcagtgccaacgatgccagtatttcgcaaggtgcttatggtaatactgcgatgattatccagaaaggttctggtaataaagcaaatattacacagtatggtactcaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K206000_sequence/1
type: Sequence
persistent identity: BBa K206000 sequence
display id: BBa_K206000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23100_sequence/1
type: Sequence
persistent identity: BBa J23100 sequence
display id: BBa_J23100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z