LOCUS       BBa_K1361007            1655 bp    DNA     linear       22-Nov-2024
DEFINITION  Curli Fiber generator under the control of Pbad promoter with CsgA 
            modified by His tag at a relative
ACCESSION   BBa_K1361007
VERSION     BBa_K1361007.1  
FEATURES             Location/Qualifiers
     promoter        1..131
                     /label=promoter
     misc_feature    40..57
                     /label=AraI1
     misc_feature    61..78
                     /label=AraI2
     promoter        1..130
                     /label=BBa_K206000
     misc_feature    143..146
                     /label=conserved
     RBS             139..150
                     /label=BBa_B0034
     misc_feature    157..159
                     /label=start
     misc_feature    610..612
                     /label=stop
     CDS             157..612
                     /label=CsgB
     CDS             157..612
                     /label=BBa_K1361997
     stem_loop       632..675
                     /label=stem_loop
     misc_feature    621..700
                     /label=BBa_B0010
     terminator      621..700
                     /label=BBa_B0010
     stem_loop       716..735
                     /label=T7 TE
     misc_feature    742..742
                     /label=stop
     polyA_site      736..749
                     /label=polya
     misc_feature    709..749
                     /label=BBa_B0012
     terminator      709..749
                     /label=BBa_B0012
     terminator      621..749
                     /label=BBa_B0015
     promoter        758..792
                     /label=BBa_J23100
     misc_feature    805..808
                     /label=conserved
     RBS             801..812
                     /label=BBa_B0034
     misc_feature    758..812
                     /label=BBa_K880005
     CDS             819..1316
                     /label=cds
     misc_feature    945..965
                     /label=7XHis
     misc_feature    1293..1313
                     /label=7XHis
     CDS             819..1316
                     /label=BBa_K1361994
     CDS             1323..1655
                     /label=cds
     misc_feature    1323..1325
                     /label=start
     misc_feature    1653..1655
                     /label=stop
     CDS             1323..1655
                     /label=BBa_K1361993
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcaagatag tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagaa agaggagaaa tactagatga aaaacaaatt gttatttatg
      181 atgttaacaa tactgggtgc gcctgggatt gcagccgcag caggttatga tttagctaat
      241 tcagaatata acttcgcggt aaatgaattg agtaagtctt catttaatca ggcagccata
      301 attggtcaag ctgggactaa taatagtgct cagttacggc agggaggctc aaaacttttg
      361 gcggttgttg cgcaagaagg tagtagcaac cgggcaaaga ttgaccagac aggagattat
      421 aaccttgcat atattgatca ggcgggcagt gccaacgatg ccagtatttc gcaaggtgct
      481 tatggtaata ctgcgatgat tatccagaaa ggttctggta ataaagcaaa tattacacag
      541 tatggtactc aaaaaacggc aattgtagtg cagagacagt cgcaaatggc tattcgcgtg
      601 acacaacgtt aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg
      661 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
      721 accttcgggt gggcctttct gcgtttatat actagagttg acggctagct cagtcctagg
      781 tacagtgcta gctactagag aaagaggaga aatactagat gaaactttta aaagtagcag
      841 caattgcagc aatcgtattc tccggtagcg ctctggcagg tgttgttcct cagtacggcg
      901 gcggcggtaa ccacggtggt ggcggtaata atagcggccc aaatcaccat caccatcacc
      961 accattctga gctgaacatt taccagtacg gtggcggtaa ctctgcactt gctctgcaaa
     1021 ctgatgcccg taactctgac ttgactatta cccagcatgg cggcggtaat ggtgcagatg
     1081 ttggtcaggg ctcagatgac agctcaatcg atctgaccca acgtggcttc ggtaacagcg
     1141 ctactcttga tcagtggaac ggcaaaaatt ctgaaatgac ggttaaacag ttcggtggtg
     1201 gcaacggtgc cgcagttgac cagactgcat ctaactcctc cgtcaacgtg actcaggttg
     1261 gctttggtaa caacgcgacc gctcatcagt accaccatca ccatcaccac cattaatact
     1321 agatgaatac gttattactc cttgcggcac tttccagtca gataaccttt aatacgaccc
     1381 agcaagggga tgtgtatacc attattcctg aagtcactct tactcaatct tgtctgtgca
     1441 gagtacaaat attgtccctg cgcgaaggca gttcagggca aagtcagacg aagcaagaaa
     1501 agaccctttc attgcctgct aatcaaccca ttgctttgac gaagttgagt ttaaatattt
     1561 ccccggacga tcgggtgaaa atagttgtta ctgtttctga tggacagtca cttcatttat
     1621 cacaacaatg gccgccctct tcagaaaagt cttaa
//