BBa_K1361007
type: ComponentDefinition
persistent identity: BBa K1361007
display id: BBa_K1361007
version: 1
was generated by: iGEM to SBOL conversion
description: Curli Fiber generator under the control of Pbad promoter with CsgA modified by His tag at a relative
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:04Z
mutable provenance: CsgB and CsgC were collected by PCR from genomic DNA of E coli. DH5alpha strain. CsgA-his was synthesized by GeneScrip.
owned by: james
owned by: myers
mutable description: This part contains the major subunit of curli fiber CsgA-his and its nucleator CsgB, in which CsgA-his is constitutive expression whereas CsgB is under the control of Pbad promotor. This part is designed to be used for immediate generate of large amount of curli fiber after adding of the inducer, and also can be used for purification of CsgA by Ni-NTA reign, or absorbing Au-NTA-Ni nano partical. This part cannot functioning alone without expression of CsgEFG genes in the same cell, for they coding the outer membrane channel secrete system for CsgA and CsgB.
discontinued: false
dominant: false
group u list: _1737_
m user id: 0
owner id: 16915
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: The modifying of CsgA was referring the supplementary of Lu, Michelle Y., et al. "Synthesis and patterning of tunable multiscale materials with engineered cells." (2014).
star: false
creator: Shoujie Sun
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K1361997
component: BBa_B0034
component: BBa_K206000
component: BBa_K1361993
component: BBa_K1361994
component: BBa_K880005
component: BBa_B0015
sequence annotation: BBa_K206000
sequence annotation: BBa_B0034
sequence annotation: BBa_K1361997
sequence annotation: BBa_B0015
sequence annotation: BBa_K880005
sequence annotation: BBa_K1361994
sequence annotation: BBa_K1361993
sequence: https://synbiohub.org/public/igem/BBa_K1361007_sequence/1

BBa_K1361997
type: Component
persistent identity: component2398632
display id: component2398632
version: 1
top level: BBa_K1361007
definition: BBa_K1361997
access: public

BBa_B0034
type: Component
persistent identity: component2398628
display id: component2398628
version: 1
top level: BBa_K1361007
definition: BBa_B0034
access: public

BBa_K206000
type: Component
persistent identity: component2398626
display id: component2398626
version: 1
top level: BBa_K1361007
definition: pBAD
access: public

BBa_K1361993
type: Component
persistent identity: component2398651
display id: component2398651
version: 1
top level: BBa_K1361007
definition: BBa_K1361993
access: public

BBa_K1361994
type: Component
persistent identity: component2398647
display id: component2398647
version: 1
top level: BBa_K1361007
definition: BBa_K1361994
access: public

BBa_K880005
type: Component
persistent identity: component2398643
display id: component2398643
version: 1
top level: BBa_K1361007
definition: BBa_K880005
access: public

BBa_B0015
type: Component
persistent identity: component2398639
display id: component2398639
version: 1
top level: BBa_K1361007
definition: BBa_B0015
access: public

BBa_K206000
type: SequenceAnnotation
persistent identity: annotation2398626
display id: annotation2398626
version: 1
top level: BBa_K1361007
location: https://synbiohub.org/public/igem/BBa_K1361007/annotation2398626/range2398626/1
component: BBa_K206000

https://synbiohub.org/public/igem/BBa_K1361007/annotation2398626/range2398626/1
type: Range
persistent identity: range2398626
display id: range2398626
version: 1
top level: BBa_K1361007
direction: direction/reverse
start: 1
end: 130
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2398628
display id: annotation2398628
version: 1
top level: BBa_K1361007
location: https://synbiohub.org/public/igem/BBa_K1361007/annotation2398628/range2398628/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1361007/annotation2398628/range2398628/1
type: Range
persistent identity: range2398628
display id: range2398628
version: 1
top level: BBa_K1361007
direction: direction/reverse
start: 139
end: 150
orientation: inline

BBa_K1361997
type: SequenceAnnotation
persistent identity: annotation2398632
display id: annotation2398632
version: 1
top level: BBa_K1361007
location: https://synbiohub.org/public/igem/BBa_K1361007/annotation2398632/range2398632/1
component: BBa_K1361997

https://synbiohub.org/public/igem/BBa_K1361007/annotation2398632/range2398632/1
type: Range
persistent identity: range2398632
display id: range2398632
version: 1
top level: BBa_K1361007
direction: direction/reverse
start: 157
end: 612
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2398639
display id: annotation2398639
version: 1
top level: BBa_K1361007
location: https://synbiohub.org/public/igem/BBa_K1361007/annotation2398639/range2398639/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1361007/annotation2398639/range2398639/1
type: Range
persistent identity: range2398639
display id: range2398639
version: 1
top level: BBa_K1361007
direction: direction/reverse
start: 621
end: 749
orientation: inline

BBa_K880005
type: SequenceAnnotation
persistent identity: annotation2398643
display id: annotation2398643
version: 1
top level: BBa_K1361007
location: https://synbiohub.org/public/igem/BBa_K1361007/annotation2398643/range2398643/1
component: BBa_K880005

https://synbiohub.org/public/igem/BBa_K1361007/annotation2398643/range2398643/1
type: Range
persistent identity: range2398643
display id: range2398643
version: 1
top level: BBa_K1361007
direction: direction/reverse
start: 758
end: 812
orientation: inline

BBa_K1361994
type: SequenceAnnotation
persistent identity: annotation2398647
display id: annotation2398647
version: 1
top level: BBa_K1361007
location: https://synbiohub.org/public/igem/BBa_K1361007/annotation2398647/range2398647/1
component: BBa_K1361994

https://synbiohub.org/public/igem/BBa_K1361007/annotation2398647/range2398647/1
type: Range
persistent identity: range2398647
display id: range2398647
version: 1
top level: BBa_K1361007
direction: direction/reverse
start: 819
end: 1316
orientation: inline

BBa_K1361993
type: SequenceAnnotation
persistent identity: annotation2398651
display id: annotation2398651
version: 1
top level: BBa_K1361007
location: https://synbiohub.org/public/igem/BBa_K1361007/annotation2398651/range2398651/1
component: BBa_K1361993

https://synbiohub.org/public/igem/BBa_K1361007/annotation2398651/range2398651/1
type: Range
persistent identity: range2398651
display id: range2398651
version: 1
top level: BBa_K1361007
direction: direction/reverse
start: 1323
end: 1655
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_K880005
type: ComponentDefinition
persistent identity: BBa K880005
display id: BBa_K880005
version: 1
was generated by: iGEM to SBOL conversion
description: Strong promoter, strong RBS combination for high expression levels of proteins
created: 2012-09-28T11:00:00Z
modified: 2015-05-08T01:13:40Z
mutable provenance: It is a combination of strong promoter (J23100) and RBS (B0034).
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -J23100+B0034 -Strong promoter, strong RBS combination for high expression levels of proteins Consensus constitutive promoter and RBS sequence-produces strongest possible expression.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1142_
m user id: 0
owner id: 9403
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Enter design considerations.
star: false
creator: Josh Atkinson, Mike Ferguson, and Ben Parker
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_B0034
component: BBa_J23100
sequence annotation: BBa_J23100
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K880005_sequence/1

BBa_B0034
type: Component
persistent identity: component2204230
display id: component2204230
version: 1
top level: BBa_K880005
definition: BBa_B0034
access: public

BBa_J23100
type: Component
persistent identity: component2204228
display id: component2204228
version: 1
top level: BBa_K880005
definition: BBa_J23100
access: public

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2204228
display id: annotation2204228
version: 1
top level: BBa_K880005
location: https://synbiohub.org/public/igem/BBa_K880005/annotation2204228/range2204228/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K880005/annotation2204228/range2204228/1
type: Range
persistent identity: range2204228
display id: range2204228
version: 1
top level: BBa_K880005
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2204230
display id: annotation2204230
version: 1
top level: BBa_K880005
location: https://synbiohub.org/public/igem/BBa_K880005/annotation2204230/range2204230/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K880005/annotation2204230/range2204230/1
type: Range
persistent identity: range2204230
display id: range2204230
version: 1
top level: BBa_K880005
direction: direction/reverse
start: 44
end: 55
orientation: inline

BBa_K1361997
type: ComponentDefinition
persistent identity: BBa K1361997
display id: BBa_K1361997
version: 1
was generated by: iGEM to SBOL conversion
description: CsgB, curlin nucleator protein, minor subunit in curli complex
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:04Z
mutable provenance: PCR on genomic DNA from E coli. DH5alpha strain.
owned by: james
owned by: myers
mutable description: CsgB is a secretive protein that attached cells' outer membranes. It plays key role in curli fiber polymerization. CsgB is the nucleator for CsgA aggregation in vivo, that facilitate the transformation of soluble CsgA into polymer fiber.
discontinued: false
dominant: false
group u list: _1737_
m user id: 0
owner id: 16915
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: CsgB can be designed under inductively control while make CsgACEFG genes constitutively express respectly. Using this method could achieve the immediate synthesis of curli fiber after inducer has been added.
star: false
creator: Shoujie Sun
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: CsgB
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K1361997_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2392539
display id: annotation2392539
version: 1
top level: BBa_K1361997
location: https://synbiohub.org/public/igem/BBa_K1361997/annotation2392539/range2392539/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K1361997/annotation2392539/range2392539/1
type: Range
persistent identity: range2392539
display id: range2392539
version: 1
top level: BBa_K1361997
direction: direction/forward
start: 1
end: 3
orientation: inline

CsgB
type: SequenceAnnotation
persistent identity: annotation2392541
display id: annotation2392541
version: 1
top level: BBa_K1361997
location: https://synbiohub.org/public/igem/BBa_K1361997/annotation2392541/range2392541/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1361997/annotation2392541/range2392541/1
type: Range
persistent identity: range2392541
display id: range2392541
version: 1
top level: BBa_K1361997
direction: direction/forward
start: 1
end: 456
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2392540
display id: annotation2392540
version: 1
top level: BBa_K1361997
location: https://synbiohub.org/public/igem/BBa_K1361997/annotation2392540/range2392540/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K1361997/annotation2392540/range2392540/1
type: Range
persistent identity: range2392540
display id: range2392540
version: 1
top level: BBa_K1361997
direction: direction/forward
start: 454
end: 456
orientation: inline

BBa_K1361994
type: ComponentDefinition
persistent identity: BBa K1361994
display id: BBa_K1361994
version: 1
was generated by: iGEM to SBOL conversion
description: Curli Fiber major monomer-CsgA with his tag
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:04Z
mutable provenance: CsgA-his was synthesized by GeneScrip.
owned by: james
owned by: myers
mutable description: This part is CsgA(BBa_K1361999) with twin 7Xhis tag. And can be used for purified of CsgA monomer or absorbing Au-NTA-Ni nano partical. CsgA is the major monomer of curli fiber. In vivo, its alone has little polymerizability and CsgB protein is requested for quick polymerization of CsgA.
discontinued: false
dominant: false
group u list: _1737_
m user id: 0
owner id: 16915
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The modifying of CsgA was referring the supplementary of Lu, Michelle Y., et al. "Synthesis and patterning of tunable multiscale materials with engineered cells." (2014).
star: false
creator: Shoujie Sun
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cds
sequence annotation: 7XHis
sequence: https://synbiohub.org/public/igem/BBa_K1361994_sequence/1

cds
type: SequenceAnnotation
persistent identity: annotation2397393
display id: annotation2397393
version: 1
top level: BBa_K1361994
location: https://synbiohub.org/public/igem/BBa_K1361994/annotation2397393/range2397393/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1361994/annotation2397393/range2397393/1
type: Range
persistent identity: range2397393
display id: range2397393
version: 1
top level: BBa_K1361994
direction: direction/forward
start: 1
end: 498
orientation: inline

7XHis
type: SequenceAnnotation
persistent identity: annotation2397394
display id: annotation2397394
version: 1
top level: BBa_K1361994
location: https://synbiohub.org/public/igem/BBa_K1361994/annotation2397394/range2397394/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_K1361994/annotation2397394/range2397394/1
type: Range
persistent identity: range2397394
display id: range2397394
version: 1
top level: BBa_K1361994
direction: direction/forward
start: 127
end: 147
orientation: inline

7XHis
type: SequenceAnnotation
persistent identity: annotation2397395
display id: annotation2397395
version: 1
top level: BBa_K1361994
location: https://synbiohub.org/public/igem/BBa_K1361994/annotation2397395/range2397395/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_K1361994/annotation2397395/range2397395/1
type: Range
persistent identity: range2397395
display id: range2397395
version: 1
top level: BBa_K1361994
direction: direction/forward
start: 475
end: 495
orientation: inline

BBa_K1361993
type: ComponentDefinition
persistent identity: BBa K1361993
display id: BBa_K1361993
version: 1
was generated by: iGEM to SBOL conversion
description: CsgC, curli fiber secrete accessory molecules
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:04Z
mutable provenance: CsgC were collected by PCR from genomic DNA of E coli. DH5alpha strain.
owned by: james
owned by: myers
mutable description: Within the periplasm, CsgC may regulate CsgG outer membrane assembly and pore activity through modification of C230 in CsgG. And its essential for curli fiber assembly.
discontinued: false
dominant: false
group u list: _1737_
m user id: 0
owner id: 16915
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Shoujie Sun
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cds
sequence annotation: start
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K1361993_sequence/1

cds
type: SequenceAnnotation
persistent identity: annotation2397399
display id: annotation2397399
version: 1
top level: BBa_K1361993
location: https://synbiohub.org/public/igem/BBa_K1361993/annotation2397399/range2397399/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1361993/annotation2397399/range2397399/1
type: Range
persistent identity: range2397399
display id: range2397399
version: 1
top level: BBa_K1361993
direction: direction/forward
start: 1
end: 333
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2397400
display id: annotation2397400
version: 1
top level: BBa_K1361993
location: https://synbiohub.org/public/igem/BBa_K1361993/annotation2397400/range2397400/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K1361993/annotation2397400/range2397400/1
type: Range
persistent identity: range2397400
display id: range2397400
version: 1
top level: BBa_K1361993
direction: direction/forward
start: 1
end: 3
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2397401
display id: annotation2397401
version: 1
top level: BBa_K1361993
location: https://synbiohub.org/public/igem/BBa_K1361993/annotation2397401/range2397401/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K1361993/annotation2397401/range2397401/1
type: Range
persistent identity: range2397401
display id: range2397401
version: 1
top level: BBa_K1361993
direction: direction/forward
start: 331
end: 333
orientation: inline

pBAD
type: ComponentDefinition
persistent identity: BBa K206000
display id: BBa_K206000
version: 1
was generated by: iGEM to SBOL conversion
description: pBAD strong
created: 2009-10-13T11:00:00Z
modified: 2015-05-08T01:11:23Z
mutable provenance: The sequence was obtained by applying all mutations that upregulated AraC binding and subsequent promoter activity listed in reference [1].
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: pBAD is an E.coli promoter that is induced by L-arabinose. K206000 is a mutagenized pBAD promoter that is responsive to lower concentrations of arabinose than wild type (<partinfo>I13453</partinfo>) and, additionally, exhibits a higher maximum of protein expression as measured by coupling to a fluorescent reporter.
discontinued: false
dominant: false
experience: experience/Works
group u list: _307_
m user id: 0
owner id: 4172
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: There were no special design considerations.
star: false
creator: Amelia Hardjasa
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: promoter
sequence annotation: AraI1
sequence annotation: AraI2
sequence: https://synbiohub.org/public/igem/BBa_K206000_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation2049252
display id: annotation2049252
version: 1
top level: pBAD
location: https://synbiohub.org/public/igem/BBa_K206000/annotation2049252/range2049252/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K206000/annotation2049252/range2049252/1
type: Range
persistent identity: range2049252
display id: range2049252
version: 1
top level: pBAD
direction: direction/forward
start: 1
end: 131
orientation: inline

AraI1
type: SequenceAnnotation
persistent identity: annotation2049253
display id: annotation2049253
version: 1
top level: pBAD
location: https://synbiohub.org/public/igem/BBa_K206000/annotation2049253/range2049253/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_K206000/annotation2049253/range2049253/1
type: Range
persistent identity: range2049253
display id: range2049253
version: 1
top level: pBAD
direction: direction/forward
start: 40
end: 57
orientation: inline

AraI2
type: SequenceAnnotation
persistent identity: annotation2049254
display id: annotation2049254
version: 1
top level: pBAD
location: https://synbiohub.org/public/igem/BBa_K206000/annotation2049254/range2049254/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_K206000/annotation2049254/range2049254/1
type: Range
persistent identity: range2049254
display id: range2049254
version: 1
top level: pBAD
direction: direction/forward
start: 61
end: 78
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_J23100
type: ComponentDefinition
persistent identity: BBa J23100
display id: BBa_J23100
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Replace later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_J23100_sequence/1

https://synbiohub.org/public/igem/BBa_K1361007_sequence/1
type: Sequence
persistent identity: BBa K1361007 sequence
display id: BBa_K1361007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatgaaaaacaaattgttatttatgatgttaacaatactgggtgcgcctgggattgcagccgcagcaggttatgatttagctaattcagaatataacttcgcggtaaatgaattgagtaagtcttcatttaatcaggcagccataattggtcaagctgggactaataatagtgctcagttacggcagggaggctcaaaacttttggcggttgttgcgcaagaaggtagtagcaaccgggcaaagattgaccagacaggagattataaccttgcatatattgatcaggcgggcagtgccaacgatgccagtatttcgcaaggtgcttatggtaatactgcgatgattatccagaaaggttctggtaataaagcaaatattacacagtatggtactcaaaaaacggcaattgtagtgcagagacagtcgcaaatggctattcgcgtgacacaacgttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgaaacttttaaaagtagcagcaattgcagcaatcgtattctccggtagcgctctggcaggtgttgttcctcagtacggcggcggcggtaaccacggtggtggcggtaataatagcggcccaaatcaccatcaccatcaccaccattctgagctgaacatttaccagtacggtggcggtaactctgcacttgctctgcaaactgatgcccgtaactctgacttgactattacccagcatggcggcggtaatggtgcagatgttggtcagggctcagatgacagctcaatcgatctgacccaacgtggcttcggtaacagcgctactcttgatcagtggaacggcaaaaattctgaaatgacggttaaacagttcggtggtggcaacggtgccgcagttgaccagactgcatctaactcctccgtcaacgtgactcaggttggctttggtaacaacgcgaccgctcatcagtaccaccatcaccatcaccaccattaatactagatgaatacgttattactccttgcggcactttccagtcagataacctttaatacgacccagcaaggggatgtgtataccattattcctgaagtcactcttactcaatcttgtctgtgcagagtacaaatattgtccctgcgcgaaggcagttcagggcaaagtcagacgaagcaagaaaagaccctttcattgcctgctaatcaacccattgctttgacgaagttgagtttaaatatttccccggacgatcgggtgaaaatagttgttactgtttctgatggacagtcacttcatttatcacaacaatggccgccctcttcagaaaagtcttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K880005_sequence/1
type: Sequence
persistent identity: BBa K880005 sequence
display id: BBa_K880005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1361997_sequence/1
type: Sequence
persistent identity: BBa K1361997 sequence
display id: BBa_K1361997_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaacaaattgttatttatgatgttaacaatactgggtgcgcctgggattgcagccgcagcaggttatgatttagctaattcagaatataacttcgcggtaaatgaattgagtaagtcttcatttaatcaggcagccataattggtcaagctgggactaataatagtgctcagttacggcagggaggctcaaaacttttggcggttgttgcgcaagaaggtagtagcaaccgggcaaagattgaccagacaggagattataaccttgcatatattgatcaggcgggcagtgccaacgatgccagtatttcgcaaggtgcttatggtaatactgcgatgattatccagaaaggttctggtaataaagcaaatattacacagtatggtactcaaaaaacggcaattgtagtgcagagacagtcgcaaatggctattcgcgtgacacaacgttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1361994_sequence/1
type: Sequence
persistent identity: BBa K1361994 sequence
display id: BBa_K1361994_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaacttttaaaagtagcagcaattgcagcaatcgtattctccggtagcgctctggcaggtgttgttcctcagtacggcggcggcggtaaccacggtggtggcggtaataatagcggcccaaatcaccatcaccatcaccaccattctgagctgaacatttaccagtacggtggcggtaactctgcacttgctctgcaaactgatgcccgtaactctgacttgactattacccagcatggcggcggtaatggtgcagatgttggtcagggctcagatgacagctcaatcgatctgacccaacgtggcttcggtaacagcgctactcttgatcagtggaacggcaaaaattctgaaatgacggttaaacagttcggtggtggcaacggtgccgcagttgaccagactgcatctaactcctccgtcaacgtgactcaggttggctttggtaacaacgcgaccgctcatcagtaccaccatcaccatcaccaccattaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1361993_sequence/1
type: Sequence
persistent identity: BBa K1361993 sequence
display id: BBa_K1361993_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaatacgttattactccttgcggcactttccagtcagataacctttaatacgacccagcaaggggatgtgtataccattattcctgaagtcactcttactcaatcttgtctgtgcagagtacaaatattgtccctgcgcgaaggcagttcagggcaaagtcagacgaagcaagaaaagaccctttcattgcctgctaatcaacccattgctttgacgaagttgagtttaaatatttccccggacgatcgggtgaaaatagttgttactgtttctgatggacagtcacttcatttatcacaacaatggccgccctcttcagaaaagtcttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K206000_sequence/1
type: Sequence
persistent identity: BBa K206000 sequence
display id: BBa_K206000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23100_sequence/1
type: Sequence
persistent identity: BBa J23100 sequence
display id: BBa_J23100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z