BBa_K1361992 1 BBa_K1361992 CsgE, CsgF, CsgG, the outer membrane secrete device for curli fiber 2014-10-05T11:00:00Z 2015-05-08T01:10:04Z PCR on genomic DNA from E coli. DH5alpha strain. And site-directed mutation by overlap PCR primers. This part is for specific transport of CsgA(BBa_K1361999) and CsgB(BBa_K1361997) into extracellular matrix. CsgE is a small periplasmic protein, is proposed to direct CsgA to the CsgG secretion complex and helps mediate secretion specificity via an N-terminal sequence in the mature CsgA. CsgF is a membrane-associated protein, required for CsgB surface exposure, and is crucial for efficient CsgA polymerization. CsgG is a canal protein at outer membrane that is required for secretion of CsgA and CsgB into the extracellular milieu. false false _1737_ 0 16915 9 Not in stock false The CsgEFG was PCRed as a whole, despite there may be some regulation sequence between them. The EcoRI loci within CsgA sequence has been replaced from GAATTC to GAATTT. false Shoujie Sun annotation2397607 1 stop range2397607 1 829 831 annotation2397602 1 CsgF range2397602 1 415 831 annotation2397603 1 CsgG range2397603 1 858 1691 annotation2397605 1 stop range2397605 1 388 390 annotation2397601 1 CsgE range2397601 1 1 390 annotation2397604 1 start range2397604 1 1 3 annotation2397606 1 start range2397606 1 415 417 annotation2397609 1 stop range2397609 1 1689 1691 annotation2397608 1 start range2397608 1 858 860 BBa_K1361992_sequence 1 atgaaacgttatttacgctggattgtggcggcagaatttctgttcgccgcagggaatcttcacgccgttgaggtagaagtcccgggattgctaactgaccatactgtttcatctattggccatgatttttaccgagcctttagtgataaatgggaaagtgactatacgggtaacttaacgattaatgaaaggcccagtgcacgatggggaagctggatcactataacggtcaatcaggacgttattttccagacttttttatttccgttgaaaagagacttcgagaaaactgtcgtctttgcactgattcaaactgaagaagcactaaatcgtcgccagataaatcaggcgttattaagtacgggcgatttggcgcatgatgaattttaaataaaaaattgttcggaggctgcaatgcgtgtcaaacatgcagtagttctactcatgcttatttcgccattaagttgggctggaaccatgactttccagttccgtaatccaaactttggtggtaacccaaataatggcgcttttttattaaatagcgctcaggcccaaaactcttataaagatccgagctataacgatgactttggtattgaaacaccctcagcgttagataactttactcaggccatccagtcacaaattttaggtgggctactgtcgaatattaataccggtaaaccgggccgcatggtgaccaacgattatattgtcgatattgccaaccgcgatggtcaattgcagttgaacgtgacagatcgtaaaaccggacaaacctcgaccatccaggtttcgggtttacaaaataactcaaccgatttttaagccccagcttcataaggaaaataatcatgcagcgcttatttcttttggttgccgtcatgttactgagcggatgcttaaccgccccgcctaaagaagccgccagaccgacattaatgcctcgtgctcagagctacaaagatttgacccatctgccagcgccgacgggtaaaatctttgtttcggtatacaacattcaggacgaaaccgggcaatttaaaccctacccggcaagtaacttctccactgctgttccgcaaagcgccacggcaatgctggtcacggcactgaaagattctcgctggtttataccgctggagcgccagggcttacaaaacctgcttaacgagcgcaagattattcgtgcggcacaagaaaacggcacggttgccattaataaccgaatcccgctgcaatctttaacggcggcaaatatcatggttgaaggttcgattatcggttatgaaagcaacgtcaaatctggcggggttggggcaagatattttggcatcggtgccgacacgcaataccagctcgatcagattgccgtgaacctgcgcgtcgtcaatgtgagtaccggcgagatcctttcttcggtgaacaccagtaagacgatactttcctatgaagttcaggccggggttttccgctttattgactaccagcgcttgcttgaaggggaagtgggttacacctcgaacgaacctgttatgctgtgcctgatgtcggctatcgaaacaggggtcattttcctgattaatgatggtatcgaccgtggtctgtgggatttgcaaaataaagcagaacggcagaatgacattctggtgaaataccgccatatgtcggttccaccggaatcctga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z