LOCUS       BBa_K1362000            1562 bp    DNA     linear       22-Dec-2024
DEFINITION  NpuDnaE intein RFC[105] circularization construct
ACCESSION   BBa_K1362000
VERSION     BBa_K1362000.1  
FEATURES             Location/Qualifiers
     RBS             11..28
                     /label=t7 RBS
     misc_feature    21..28
                     /label=Shine-Dalgarno
     RBS             1..29
                     /label=BBa_K1362090
     misc_feature    30..35
                     /label=BBa_G0000
     misc_feature    36..38
                     /label=BBa_K1362414
     misc_feature    39..139
                     /label=BBa_K1362401
     misc_feature    140..143
                     /label=BBa_K1362419
     misc_feature    144..150
                     /label=BBa_K1362423
     misc_feature    151..1219
                     /label=BBa_J04550*
     misc_feature    151..1219
                     /label=BBa_K1362505
     misc_feature    1220..1226
                     /label=BBa_K1362424
     misc_feature    1227..1230
                     /label=BBa_K1362415
     misc_feature    1231..1532
                     /label=BBa_K1362400
     misc_feature    1533..1536
                     /label=stop
     misc_feature    1533..1536
                     /label=BBa_K1362421
     misc_feature    1560..1562
                     /label=stop
     misc_feature    1557..1559
                     /label=stop
     misc_feature    1539..1556
                     /label=iGEMHD
     misc_feature    1537..1562
                     /label=BBa_K1362999
ORIGIN
        1 aataattttg tttaacttta agaaggagat actagatgat caaaatagcc acacgtaaat
       61 atttaggcaa acaaaatgtc tatgacattg gagttgagcg cgaccataat tttgcactca
      121 aaaatggctt catagcttcc aacagagacc caatacgcaa accgcctctc cccgcgcgtt
      181 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
      241 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
      301 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca tactagagaa
      361 agaggagaaa tactagatgg cttcctccga agacgttatc aaagagttca tgcgtttcaa
      421 agttcgtatg gaaggttccg ttaacggtca cgagttcgaa atcgaaggtg aaggtgaagg
      481 tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt accaaaggtg gtccgctgcc
      541 gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt tccaaagctt acgttaaaca
      601 cccggctgac atcccggact acctgaaact gtccttcccg gaaggtttca aatgggaacg
      661 tgttatgaac ttcgaagacg gtggtgttgt taccgttacc caggactcct ccctgcaaga
      721 cggtgagttc atctacaaag ttaaactgcg tggtaccaac ttcccgtccg atggtccggt
      781 tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc cggaagacgg
      841 tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc actacgacgc
      901 tgaagttaaa accacctaca tggctaaaaa accggttcag ctgccgggtg cttacaaaac
      961 cgacatcaaa ctggacatca cctcccacaa cgaagactac accatcgttg aacagtacga
     1021 acgtgctgaa ggtcgtcact ccaccggtgc ttaataacgc tgatagtgct agtgtagatc
     1081 gctactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     1141 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
     1201 gggcctttct gcgtttatag gtctcatgct taagctatga aacggaaata ttgacagtag
     1261 aatatggatt attaccgatt ggtaaaattg tagaaaagcg catcgaatgt actgtttata
     1321 gcgttgataa taatggaaat atttatacac aacctgtagc acaatggcac gatcgcggag
     1381 aacaagaggt gtttgagtat tgtttggaag atggttcatt gattcgggca acaaaagacc
     1441 ataagtttat gactgttgat ggtcaaatgt tgccaattga tgaaatattt gaacgtgaat
     1501 tggatttgat gcgggttgat aatttgccga attaataaat cggtgaaatg cacgactgat
     1561 ag
//