BBa_K1362505
1
BBa_04450*
BBa_J04450 with a mutation
2014-10-16T11:00:00Z
2015-05-08T01:10:06Z
<partinfo>BBa_J04450</partinfo>
Like <partinfo>BBa_J04450</partinfo> but with a C->T mutation at 397, resulting in aan Arg-Trp mutation. The bacteria become red anyway.
false
false
_1738_
0
22920
9
Not in stock
false
This part was designed randomly.
false
Max Waldhauer
annotation2427465
1
BBa_J04550*
range2427465
1
1
1069
BBa_K1362421
1
RFC105 Z
C-terminal stop overhang TAAT=STOP+1/3Stop RFC[105] overhang Z
2014-10-06T11:00:00Z
2015-05-08T01:10:05Z
This standard overhang part was developed as part of the iGEM team Heidelberg 2014's Intein Toolbox [[#References|[1]]]. All standard sequences can be reviewed in RFC[???] [[#References|[2]]].
This is a standard overhang sequence for in-frame cloning of Proteins of Interest in front or behind an Intein. A detailed cloning strategy is found on the iGEM team Heidelberg 2014's [http://2014.igem.org/Team:Heidelberg/parts wiki page] as well as in RFC[???]. Specifically, this part contains the overhang F used to insert the last part of an intein-fused protein directly in front of an RFC[10] double stop-codon. It lies within the first for bases of the stopstop sequence.
false
false
_1738_
0
12377
9
Not in stock
false
This part is only the sequence of a standard overhang. It will not be sent in as physical DNA and was merely created to easily compose new parts in the RFC[???] standard. In the actual cloning process this sequence was and is recommended to be inserted with the according PCR primers.
false
Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
annotation2402257
1
stop
range2402257
1
1
4
BBa_K1362415
1
RFC105 NN
N-terminal splicing overhang TGCT=Cys+1/3 Leu|Ile RFC[105] overhang NN
2014-10-06T11:00:00Z
2015-05-08T01:10:05Z
This standard overhang part was developed as part of the iGEM team Heidelberg 2014's Intein Toolbox [[#References[1]]]. All standard sequences can be reviewed in RFC[???] [[#References[2]]].
This is a standard overhang sequence for in-frame cloning of Proteins of Interest in front or behind an Intein. A detailed cloning strategy is found on the iGEM team Heidelberg 2014's [[http://2014.igem.org/Team:Heidelberg|wiki page]] as well as in RFC[???]. Specifically, this part contains the overhang A used to insert a protein behind any RFC[10] compatible RBS and in-frame with the start-codon. It lies within the four bases formed by the second last base of the XbaI/SpeI scar or the first base in front of start codon respectively and the start codon itself.
false
false
_1738_
0
12377
9
Not in stock
false
This part is only the sequence of a standard overhang. It will not be sent in as physical DNA and was merely created to easily compose new parts in the RFC[???] standard. In the actual cloning process this sequence was and is recommended to be inserted with the according PCR primers.
false
Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
BBa_K1362090
1
T7 RBS
strong T7 RBS
2014-10-02T11:00:00Z
2015-05-08T01:10:04Z
synthesized as found in the T7 genome and several commercial expression plasmids.
RFC10 compatible strong RBS derived from the T7 phage gene 10a (major capsid protein)[1]. When assembled to a coding part with the A of the start codon being part of the XbaI site, the RBS will be shifted one bp downstream compared to the native sequence.
The sequence was successfully used by the iGEM team Heidelberg 2014 for the expression of many proteins in E.coli.
1. Olinss, P. & Rangwala, S. H. Derived from Bacteriophage T7 mRNA Acts ELS an Enhancer of Translation of the lac2 Gene in. 16973???16976 (1989).
false
false
_1738_
0
22830
9
It's complicated
false
The 18 bp including the XbaI that can be found upstream of the presumably important part of the RBS were included into the sequence just to make sure it works. However to fully comply with RFC10 a G was inserted behind the XbaI site.
false
Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
annotation2393796
1
Shine-Dalgarno
range2393796
1
21
28
annotation2393795
1
t7 RBS
range2393795
1
11
28
BBa_K1362423
1
<- BsaI
BsaI reverse restriction site for RFC[105] cloning
2014-10-06T11:00:00Z
2015-05-08T01:10:05Z
This standard restriction site sequence is used as part of the iGEM team Heidelberg 2014's Intein Toolbox [[#References|[1]]]. All associated standard sequences can be reviewed in RFC[???]
This is the reverse complement of a BsaI restriction site headed by an Adenine as a spacer-base to separate the recognition sequence from the outward-lying cutting sequence. It was used by us for scarless golden-gate cloning to fuse inteins to other proteins and thereby implement a variety of possible port-translational modifications.
false
false
_1738_
0
12377
9
Not in stock
false
This part is only the sequence of a restriction site. It will not be sent in as physical DNA and was merely created to easily compose new parts in the RFC[???] standard. In the actual cloning process this sequence was and is recommended to be inserted with the according PCR primers.
false
Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
BBa_G0000
1
scar
SpeI/XbaI scar for RBS-CDS junctions
2007-07-22T11:00:00Z
2015-08-31T04:07:27Z
SpeI/XbaI scar
This is the sequence of the SpeI/XbaI scar for RBS-CDS junctions in BioBricks standard assembly.
false
true
_41_
0
126
162
Not in stock
false
This is a shorter scar to ensure proper spacing between the RBS and CDS.
false
Reshma Shetty
BBa_K1362999
1
BBa_K1362999
iGEMHD tag
2014-10-07T11:00:00Z
2015-05-08T01:10:06Z
Directly synthesized as an oligo as backtranslated with EMBOSS[1].
==References==
1. Rice, P., Longden, I. & Bleasby, A. EMBOSS: the European Molecular Biology Open Software Suite. Trends Genet. 16, 276???7 (2000).
This Part adds ultimate coolness to you your part.
More seriously, it served us as a random sequence being a better overlap site for CPEC cloning than the RFC[10] suffix.
false
false
_1738_
0
22830
9
Not in stock
false
We aimed for a sequence without secondary structures, balanced GC content.
false
Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
annotation2400473
1
stop
range2400473
1
21
23
annotation2400474
1
iGEMHD
range2400474
1
3
20
annotation2400472
1
stop
range2400472
1
24
26
BBa_K1362414
1
RFC105 A
N-terminal start overhang (T)(A)-(G)ATG=RBS+Start RFC[105] A
2014-10-06T11:00:00Z
2015-05-08T01:10:05Z
This standard overhang part was developed as part of the iGEM team Heidelberg 2014's Intein Toolbox [[#References[1]]]. All standard sequences can be reviewed in RFC[???] [[#References[2]]].
This is a standard overhang sequence for in-frame cloning of Proteins of Interest in front or behind an Intein. A detailed cloning strategy is found on the iGEM team Heidelberg 2014's [[http://2014.igem.org/Team:Heidelberg|wiki page]] as well as in RFC[???]. Specifically, this part contains the overhang A used to insert a protein behind any RFC[10] compatible RBS and in-frame with the start-codon. It lies within the four bases formed by the second last base of the XbaI/SpeI scar or the first base in front of start codon respectively and the start codon itself.
false
false
_1738_
0
12377
9
Not in stock
false
This part is only the sequence of a standard overhang. It will not be sent in as physical DNA and was merely created to easily compose new parts in the RFC[???] standard. In the actual cloning process this sequence was and is recommended to be inserted with the according PCR primers.
false
Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
BBa_K1362400
1
NpuDnaE(N)
NpuDnaE N-Intein cloning piece
2014-10-05T11:00:00Z
2015-05-08T01:10:05Z
Obtained from pVS07 by Prof. Henning D. Mootz, University of Muenster.
Nostoc punctiforme DnaE split Intein N-terminal half. This is a DNA piece for cloning used to assemble other BioBrick parts.
false
false
_1738_
0
12377
9
Not in stock
false
This part represents only the intein sequence without inluding the standard splicing-site or polyglycine-linker overhangs.
false
Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
BBa_K1362424
1
BsaI ->
BsaI restriction site for RFC[105] cloning
2014-10-06T11:00:00Z
2015-05-08T01:10:05Z
This standard restriction site sequence is used as part of the iGEM team Heidelberg 2014's Intein Toolbox [[#References|[1]]]. All associated standard sequences can be reviewed in RFC[???]
This is the reverse complement of a BsaI restriction site headed by an Adenine as a spacer-base to separate the recognition sequence from the outward-lying cutting sequence. It was used by us for scarless golden-gate cloning to fuse inteins to other proteins and thereby implement a variety of possible port-translational modifications.
false
false
_1738_
0
12377
9
Not in stock
false
This part is only the sequence of a restriction site. It will not be sent in as physical DNA and was merely created to easily compose new parts in the RFC[???] standard. In the actual cloning process this sequence was and is recommended to be inserted with the according PCR primers.
false
Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
BBa_K1362000
1
CircNpu
NpuDnaE intein RFC[105] circularization construct
2014-10-06T11:00:00Z
2015-05-08T01:10:04Z
???
???
false
false
_1738_
0
22830
9
In stock
true
???
false
Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Buescher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schaefer, Carolin Schmelas, Silvan Schmitz, Max Waldhauer
component2427487
1
BBa_K1362999
component2427481
1
BBa_K1362400
component2427478
1
BBa_K1362505
component2427472
1
BBa_G0000
component2427480
1
BBa_K1362415
component2427473
1
BBa_K1362414
component2427475
1
BBa_K1362419
component2427471
1
BBa_K1362090
component2427474
1
BBa_K1362401
component2427479
1
BBa_K1362424
component2427476
1
BBa_K1362423
component2427483
1
BBa_K1362421
annotation2427473
1
BBa_K1362414
range2427473
1
36
38
annotation2427472
1
BBa_G0000
range2427472
1
30
35
annotation2427479
1
BBa_K1362424
range2427479
1
1220
1226
annotation2427481
1
BBa_K1362400
range2427481
1
1231
1532
annotation2427476
1
BBa_K1362423
range2427476
1
144
150
annotation2427487
1
BBa_K1362999
range2427487
1
1537
1562
annotation2427471
1
BBa_K1362090
range2427471
1
1
29
annotation2427474
1
BBa_K1362401
range2427474
1
39
139
annotation2427483
1
BBa_K1362421
range2427483
1
1533
1536
annotation2427480
1
BBa_K1362415
range2427480
1
1227
1230
annotation2427475
1
BBa_K1362419
range2427475
1
140
143
annotation2427478
1
BBa_K1362505
range2427478
1
151
1219
BBa_K1362401
1
NpuDnaE(C)
NpuDnaE C-Intein cloning piece
2014-10-05T11:00:00Z
2015-05-08T01:10:05Z
Obtained from pVS41 by Prof. Henning D. Mootz, University of Muenster.
Nostoc punctiforme DnaE split Intein C-terminal half. This is a DNA piece for cloning used to assemble our other BioBrick parts.
false
false
_1738_
0
12377
9
Not in stock
false
This part represents only the intein sequence without inluding the standard splicing-site or polyglycine-linker overhangs.
false
Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
BBa_K1362419
1
RFC105 CC
C-terminal splicing overhang CAAC=1/3?+Asn RFC[105] standard overhang CC
2014-10-06T11:00:00Z
2015-05-08T01:10:05Z
This standard overhang part was developed as part of the iGEM team Heidelberg 2014's Intein Toolbox [[#References|[1]]]. All standard sequences can be reviewed in RFC[???] [[#References|[2]]].
This is a standard overhang sequence for in-frame cloning of Proteins of Interest in front or behind an Intein. A detailed cloning strategy is found on the iGEM team Heidelberg 2014's [http://2014.igem.org/Team:Heidelberg/parts wiki page] as well as in RFC[???]. Specifically, this part contains the overhang E used to insert a protein in front of an N-Intein. It lies within the four bases formed by the cystein and the first base of the subsequent Leucine/Isoleucine or similar of the N-terminal splicing site.
false
false
_1738_
0
12377
9
Not in stock
false
This part is only the sequence of a standard overhang. It will not be sent in as physical DNA and was merely created to easily compose new parts in the RFC[???] standard. In the actual cloning process this sequence was and is recommended to be inserted with the according PCR primers.
false
Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
BBa_K1362419_sequence
1
caac
BBa_K1362423_sequence
1
agagacc
BBa_K1362401_sequence
1
atcaaaatagccacacgtaaatatttaggcaaacaaaatgtctatgacattggagttgagcgcgaccataattttgcactcaaaaatggcttcatagcttc
BBa_K1362414_sequence
1
atg
BBa_K1362400_sequence
1
taagctatgaaacggaaatattgacagtagaatatggattattaccgattggtaaaattgtagaaaagcgcatcgaatgtactgtttatagcgttgataataatggaaatatttatacacaacctgtagcacaatggcacgatcgcggagaacaagaggtgtttgagtattgtttggaagatggttcattgattcgggcaacaaaagaccataagtttatgactgttgatggtcaaatgttgccaattgatgaaatatttgaacgtgaattggatttgatgcgggttgataatttgccgaat
BBa_K1362999_sequence
1
aaatcggtgaaatgcacgactgatag
BBa_K1362505_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgatggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K1362421_sequence
1
taat
BBa_K1362090_sequence
1
aataattttgtttaactttaagaaggaga
BBa_K1362415_sequence
1
tgct
BBa_G0000_sequence
1
tactag
BBa_K1362424_sequence
1
ggtctca
BBa_K1362000_sequence
1
aataattttgtttaactttaagaaggagatactagatgatcaaaatagccacacgtaaatatttaggcaaacaaaatgtctatgacattggagttgagcgcgaccataattttgcactcaaaaatggcttcatagcttccaacagagacccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgatggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttataggtctcatgcttaagctatgaaacggaaatattgacagtagaatatggattattaccgattggtaaaattgtagaaaagcgcatcgaatgtactgtttatagcgttgataataatggaaatatttatacacaacctgtagcacaatggcacgatcgcggagaacaagaggtgtttgagtattgtttggaagatggttcattgattcgggcaacaaaagaccataagtttatgactgttgatggtcaaatgttgccaattgatgaaatatttgaacgtgaattggatttgatgcgggttgataatttgccgaattaataaatcggtgaaatgcacgactgatag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z