LOCUS       BBa_K1362001            1880 bp    DNA     linear       22-Dec-2024
DEFINITION  NpuDnaE intein RFC[105] circularization construct (with FLAG and 
            Smt3)
ACCESSION   BBa_K1362001
VERSION     BBa_K1362001.1  
FEATURES             Location/Qualifiers
     RBS             11..28
                     /label=t7 RBS
     misc_feature    21..28
                     /label=Shine-Dalgarno
     RBS             1..29
                     /label=BBa_K1362090
     misc_feature    30..35
                     /label=BBa_G0000
     misc_feature    36..38
                     /label=BBa_K1362414
     misc_feature    39..62
                     /label=FLAG
     misc_feature    39..62
                     /label=BBa_K1362428
     misc_feature    63..356
                     /label=Smt3
     misc_feature    63..356
                     /label=BBa_K1362429
     misc_feature    357..457
                     /label=BBa_K1362401
     misc_feature    458..461
                     /label=BBa_K1362419
     misc_feature    462..468
                     /label=BBa_K1362423
     misc_feature    469..1537
                     /label=BBa_J04550*
     misc_feature    469..1537
                     /label=BBa_K1362505
     misc_feature    1538..1544
                     /label=BBa_K1362424
     misc_feature    1545..1548
                     /label=BBa_K1362415
     misc_feature    1549..1850
                     /label=BBa_K1362400
     misc_feature    1851..1854
                     /label=stop
     misc_feature    1851..1854
                     /label=BBa_K1362421
     misc_feature    1878..1880
                     /label=stop
     misc_feature    1875..1877
                     /label=stop
     misc_feature    1857..1874
                     /label=iGEMHD
     misc_feature    1855..1880
                     /label=BBa_K1362999
ORIGIN
        1 aataattttg tttaacttta agaaggagat actagatgga ctacaaagac gatgacgaca
       61 agtcggactc agaagtcaat caagaagcta agccagaggt caagccagaa gtcaagcctg
      121 agactcacat caatttaaag gtgtccgatg gatcttcaga gatcttcttc aagatcaaaa
      181 agaccactcc tttaagaagg ctgatggaag cgttcgctaa aagacagggt aaggaaatgg
      241 actccttaag attcttgtac gacggtatta gaatccaagc tgatcagacc cctgaagatt
      301 tggacatgga ggataacgat attattgagg ctcacagaga acagattggt ggatccatca
      361 aaatagccac acgtaaatat ttaggcaaac aaaatgtcta tgacattgga gttgagcgcg
      421 accataattt tgcactcaaa aatggcttca tagcttccaa cagagaccca atacgcaaac
      481 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
      541 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
      601 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
      661 ttcacacata ctagagaaag aggagaaata ctagatggct tcctccgaag acgttatcaa
      721 agagttcatg cgtttcaaag ttcgtatgga aggttccgtt aacggtcacg agttcgaaat
      781 cgaaggtgaa ggtgaaggtc gtccgtacga aggtacccag accgctaaac tgaaagttac
      841 caaaggtggt ccgctgccgt tcgcttggga catcctgtcc ccgcagttcc agtacggttc
      901 caaagcttac gttaaacacc cggctgacat cccggactac ctgaaactgt ccttcccgga
      961 aggtttcaaa tgggaacgtg ttatgaactt cgaagacggt ggtgttgtta ccgttaccca
     1021 ggactcctcc ctgcaagacg gtgagttcat ctacaaagtt aaactgcgtg gtaccaactt
     1081 cccgtccgat ggtccggtta tgcagaaaaa aaccatgggt tgggaagctt ccaccgaacg
     1141 tatgtacccg gaagacggtg ctctgaaagg tgaaatcaaa atgcgtctga aactgaaaga
     1201 cggtggtcac tacgacgctg aagttaaaac cacctacatg gctaaaaaac cggttcagct
     1261 gccgggtgct tacaaaaccg acatcaaact ggacatcacc tcccacaacg aagactacac
     1321 catcgttgaa cagtacgaac gtgctgaagg tcgtcactcc accggtgctt aataacgctg
     1381 atagtgctag tgtagatcgc tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
     1441 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
     1501 actggctcac cttcgggtgg gcctttctgc gtttataggt ctcatgctta agctatgaaa
     1561 cggaaatatt gacagtagaa tatggattat taccgattgg taaaattgta gaaaagcgca
     1621 tcgaatgtac tgtttatagc gttgataata atggaaatat ttatacacaa cctgtagcac
     1681 aatggcacga tcgcggagaa caagaggtgt ttgagtattg tttggaagat ggttcattga
     1741 ttcgggcaac aaaagaccat aagtttatga ctgttgatgg tcaaatgttg ccaattgatg
     1801 aaatatttga acgtgaattg gatttgatgc gggttgataa tttgccgaat taataaatcg
     1861 gtgaaatgca cgactgatag
//