##gff-version 3
##sequence-region BBa_K1362003 1 1477
BBa_K1362003	.	engineered_region	36	1477	.	+	0	ID=BBa_K1362203;Name=BBa_K1362203
BBa_K1362003	.	sequence_feature	1429	1435	.	+	0	ID=BBa_K1362424;Name=BBa_K1362424;Parent=BBa_K1362203
BBa_K1362003	.	tag	1454	1471	.	+	0	ID=BBa_K1362457;Name=BBa_K1362457;Parent=BBa_K1362203
BBa_K1362003	.	sequence_feature	354	359	.	+	0	ID=BBa_K1362427;Name=BBa_K1362427;Parent=BBa_K1362203
BBa_K1362003	.	polypeptide_domain	1472	1477	.	+	0	ID=BBa_J70594;Name=BBa_J70594;Parent=BBa_K1362203
BBa_K1362003	.	stop_codon	1472	1476	.	+	0	ID=annotation2071257;Name=stop;Parent=BBa_J70594
BBa_K1362003	.	polypeptide_domain	39	332	.	+	0	ID=BBa_K1362429;Name=BBa_K1362429;Parent=BBa_K1362203
BBa_K1362003	.	sequence_feature	39	332	.	+	0	ID=annotation2407301;Name=Smt3;Parent=BBa_K1362429
BBa_K1362003	.	polypeptide_domain	333	353	.	+	0	ID=BBa_K1362453;Name=BBa_K1362453;Parent=BBa_K1362203
BBa_K1362003	.	engineered_region	360	1428	.	+	0	ID=BBa_J04450;Name=BBa_J04450;Parent=BBa_K1362203
BBa_K1362003	.	ribosome_entry_site	568	579	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04450
BBa_K1362003	.	conserved_region	572	575	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1362003	.	terminator	1300	1379	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J04450
BBa_K1362003	.	stem_loop	1311	1354	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1362003	.	engineered_region	1300	1379	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1362003	.	promoter	360	559	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04450
BBa_K1362003	.	promoter	496	501	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1362003	.	start_codon	532	532	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1362003	.	non_covalent_binding_site	448	485	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1362003	.	promoter	520	525	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1362003	.	non_covalent_binding_site	525	559	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1362003	.	engineered_region	360	559	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1362003	.	CDS	360	447	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1362003	.	CDS	586	1266	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_J04450
BBa_K1362003	.	engineered_tag	1267	1291	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1362003	.	CDS	586	1260	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1362003	.	terminator	1388	1428	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J04450
BBa_K1362003	.	polyA_site	1415	1428	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1362003	.	stem_loop	1395	1414	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1362003	.	engineered_region	1388	1428	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1362003	.	stop_codon	1421	1421	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1362003	.	polypeptide_domain	36	38	.	+	0	ID=BBa_K371056;Name=BBa_K371056;Parent=BBa_K1362203
BBa_K1362003	.	start_codon	36	38	.	+	0	ID=annotation2113878;Name=start codon;Parent=BBa_K371056
BBa_K1362003	.	polypeptide_domain	1436	1453	.	+	0	ID=BBa_K1362455;Name=BBa_K1362455;Parent=BBa_K1362203
BBa_K1362003	.	ribosome_entry_site	1	29	.	+	0	ID=BBa_K1362090;Name=BBa_K1362090
BBa_K1362003	.	ribosome_entry_site	11	28	.	+	0	ID=annotation2393795;Name=t7 RBS;Parent=BBa_K1362090
BBa_K1362003	.	sequence_feature	21	28	.	+	0	ID=annotation2393796;Name=Shine-Dalgarno;Parent=BBa_K1362090
>BBa_K1362003
aataattttgtttaactttaagaaggagatactagatgtcggactcagaagtcaatcaagaagctaagccagaggtcaa
gccagaagtcaagcctgagactcacatcaatttaaaggtgtccgatggatcttcagagatcttcttcaagatcaaaaag
accactcctttaagaaggctgatggaagcgttcgctaaaagacagggtaaggaaatggactccttaagattcttgtacg
acggtattagaatccaagctgatcagacccctgaagatttggacatggaggataacgatattattgaggctcacagaga
acagattggtggatccgaaaacctgtacttccagggttgagaccaatacgcaaaccgcctctccccgcgcgttggccga
ttcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagct
cactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaattt
cacacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagt
tcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccag
accgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggtt
ccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacg
tgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaa
gttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccg
aacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacga
cgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggac
atcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaat
aacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcc
tttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcg
tttataggtctcacttccggaaaccggtggtcatcaccatcaccatcactaataa