##gff-version 3
##sequence-region BBa_K1362004 1 1195
BBa_K1362004	.	ribosome_entry_site	1	29	.	+	0	ID=BBa_K1362090;Name=BBa_K1362090
BBa_K1362004	.	sequence_feature	21	28	.	+	0	ID=annotation2393796;Name=Shine-Dalgarno;Parent=BBa_K1362090
BBa_K1362004	.	ribosome_entry_site	11	28	.	+	0	ID=annotation2393795;Name=t7 RBS;Parent=BBa_K1362090
BBa_K1362004	.	engineered_region	36	1195	.	+	0	ID=BBa_K1362204;Name=BBa_K1362204
BBa_K1362004	.	polypeptide_domain	66	74	.	+	0	ID=BBa_K1362449;Name=BBa_K1362449;Parent=BBa_K1362204
BBa_K1362004	.	polypeptide_domain	75	95	.	+	0	ID=BBa_K1362453;Name=BBa_K1362453;Parent=BBa_K1362204
BBa_K1362004	.	engineered_region	102	1170	.	+	0	ID=BBa_J04450;Name=BBa_J04450;Parent=BBa_K1362204
BBa_K1362004	.	terminator	1042	1121	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J04450
BBa_K1362004	.	engineered_region	1042	1121	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1362004	.	stem_loop	1053	1096	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1362004	.	promoter	102	301	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04450
BBa_K1362004	.	non_covalent_binding_site	190	227	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1362004	.	promoter	262	267	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1362004	.	engineered_region	102	301	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1362004	.	CDS	102	189	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1362004	.	non_covalent_binding_site	267	301	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1362004	.	promoter	238	243	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1362004	.	start_codon	274	274	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1362004	.	terminator	1130	1170	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J04450
BBa_K1362004	.	engineered_region	1130	1170	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1362004	.	stop_codon	1163	1163	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1362004	.	stem_loop	1137	1156	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1362004	.	polyA_site	1157	1170	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1362004	.	CDS	328	1008	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_J04450
BBa_K1362004	.	engineered_tag	1009	1033	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1362004	.	CDS	328	1002	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1362004	.	ribosome_entry_site	310	321	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04450
BBa_K1362004	.	conserved_region	314	317	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1362004	.	polypeptide_domain	1190	1195	.	+	0	ID=BBa_J70594;Name=BBa_J70594;Parent=BBa_K1362204
BBa_K1362004	.	stop_codon	1190	1194	.	+	0	ID=annotation2071257;Name=stop;Parent=BBa_J70594
BBa_K1362004	.	tag	48	65	.	+	0	ID=BBa_K1362458;Name=BBa_K1362458;Parent=BBa_K1362204
BBa_K1362004	.	polypeptide_domain	36	47	.	+	0	ID=BBa_K1362445;Name=BBa_K1362445;Parent=BBa_K1362204
BBa_K1362004	.	sequence_feature	1171	1177	.	+	0	ID=BBa_K1362424;Name=BBa_K1362424;Parent=BBa_K1362204
BBa_K1362004	.	sequence_feature	96	101	.	+	0	ID=BBa_K1362447;Name=BBa_K1362447;Parent=BBa_K1362204
BBa_K1362004	.	polypeptide_domain	1178	1189	.	+	0	ID=BBa_K1362459;Name=BBa_K1362459;Parent=BBa_K1362204
>BBa_K1362004
aataattttgtttaactttaagaaggagatactagatgggcagtagtcatcatcatcatcatcacagcagcggcgaaaa
cctgtacttccagggtagagaccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacg
acaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggc
tttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagagg
agaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaa
cggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttacc
aaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacc
cggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacgg
tggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaac
ttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacg
gtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccaccta
catggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagac
tacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgta
gatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttg
tcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttataggtctcacttccgga
aacctaataa