BBa_K1362004
type: ComponentDefinition
persistent identity: BBa K1362004
display id: BBa_K1362004
version: 1
was generated by: iGEM to SBOL conversion
description: Sortase A non-circularization construct (with His6)
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:10:04Z
mutable provenance: TODO
owned by: james
owned by: myers
mutable description: TODO
discontinued: false
dominant: false
group u list: _1738_
m user id: 0
owner id: 22951
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TODO
star: false
creator: Silvan Schmitz
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K1362090
component: BBa_K1362204
sequence annotation: BBa_K1362090
sequence annotation: BBa_K1362204
sequence: https://synbiohub.org/public/igem/BBa_K1362004_sequence/1

BBa_K1362090
type: Component
persistent identity: component2409455
display id: component2409455
version: 1
top level: BBa_K1362004
definition: T7 RBS
access: public

BBa_K1362204
type: Component
persistent identity: component2409483
display id: component2409483
version: 1
top level: BBa_K1362004
definition: BBa_K1362204
access: public

BBa_K1362090
type: SequenceAnnotation
persistent identity: annotation2409455
display id: annotation2409455
version: 1
top level: BBa_K1362004
location: https://synbiohub.org/public/igem/BBa_K1362004/annotation2409455/range2409455/1
component: BBa_K1362090

https://synbiohub.org/public/igem/BBa_K1362004/annotation2409455/range2409455/1
type: Range
persistent identity: range2409455
display id: range2409455
version: 1
top level: BBa_K1362004
direction: direction/reverse
start: 1
end: 29
orientation: inline

BBa_K1362204
type: SequenceAnnotation
persistent identity: annotation2409483
display id: annotation2409483
version: 1
top level: BBa_K1362004
location: https://synbiohub.org/public/igem/BBa_K1362004/annotation2409483/range2409483/1
component: BBa_K1362204

https://synbiohub.org/public/igem/BBa_K1362004/annotation2409483/range2409483/1
type: Range
persistent identity: range2409483
display id: range2409483
version: 1
top level: BBa_K1362004
direction: direction/reverse
start: 36
end: 1195
orientation: inline

T7 RBS
type: ComponentDefinition
persistent identity: BBa K1362090
display id: BBa_K1362090
version: 1
was generated by: iGEM to SBOL conversion
description: strong T7 RBS
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:04Z
mutable provenance: synthesized as found in the T7 genome and several commercial expression plasmids.
owned by: james
owned by: myers
mutable description: RFC10 compatible strong RBS derived from the T7 phage gene 10a (major capsid protein)[1]. When assembled to a coding part with the A of the start codon being part of the XbaI site, the RBS will be shifted one bp downstream compared to the native sequence. The sequence was successfully used by the iGEM team Heidelberg 2014 for the expression of many proteins in E.coli. 1. Olinss, P. & Rangwala, S. H. Derived from Bacteriophage T7 mRNA Acts ELS an Enhancer of Translation of the lac2 Gene in. 16973???16976 (1989).
discontinued: false
dominant: false
experience: experience/Works
group u list: _1738_
m user id: 0
owner id: 22830
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: The 18 bp including the XbaI that can be found upstream of the presumably important part of the RBS were included into the sequence just to make sure it works. However to fully comply with RFC10 a G was inserted behind the XbaI site.
star: false
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: t7 RBS
sequence annotation: Shine-Dalgarno
sequence: https://synbiohub.org/public/igem/BBa_K1362090_sequence/1

t7 RBS
type: SequenceAnnotation
persistent identity: annotation2393795
display id: annotation2393795
version: 1
top level: T7 RBS
location: https://synbiohub.org/public/igem/BBa_K1362090/annotation2393795/range2393795/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K1362090/annotation2393795/range2393795/1
type: Range
persistent identity: range2393795
display id: range2393795
version: 1
top level: T7 RBS
direction: direction/forward
start: 11
end: 28
orientation: inline

Shine-Dalgarno
type: SequenceAnnotation
persistent identity: annotation2393796
display id: annotation2393796
version: 1
top level: T7 RBS
location: https://synbiohub.org/public/igem/BBa_K1362090/annotation2393796/range2393796/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1362090/annotation2393796/range2393796/1
type: Range
persistent identity: range2393796
display id: range2393796
version: 1
top level: T7 RBS
direction: direction/forward
start: 21
end: 28
orientation: inline

BBa_K1362204
type: ComponentDefinition
persistent identity: BBa K1362204
display id: BBa_K1362204
version: 1
was generated by: iGEM to SBOL conversion
description: Sortase A non-circularization construct (with His6; without RBS)
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:05Z
mutable provenance: TODO
owned by: james
owned by: myers
mutable description: TODO
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 22951
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TODo
star: false
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J70594
component: BBa_K1362459
component: BBa_K1362449
component: BBa_K1362458
component: BBa_K1362445
component: BBa_K1362424
component: BBa_J04450
component: BBa_K1362447
component: BBa_K1362453
sequence annotation: BBa_K1362445
sequence annotation: BBa_K1362458
sequence annotation: BBa_K1362449
sequence annotation: BBa_K1362453
sequence annotation: BBa_K1362447
sequence annotation: BBa_J04450
sequence annotation: BBa_K1362424
sequence annotation: BBa_K1362459
sequence annotation: BBa_J70594
sequence: https://synbiohub.org/public/igem/BBa_K1362204_sequence/1

BBa_J70594
type: Component
persistent identity: component2410846
display id: component2410846
version: 1
top level: BBa_K1362204
definition: BBa_J70594
access: public

BBa_K1362459
type: Component
persistent identity: component2410844
display id: component2410844
version: 1
top level: BBa_K1362204
definition: SortA mock
access: public

BBa_K1362449
type: Component
persistent identity: component2410822
display id: component2410822
version: 1
top level: BBa_K1362204
definition: SSG
access: public

BBa_K1362458
type: Component
persistent identity: component2410821
display id: component2410821
version: 1
top level: BBa_K1362204
definition: His6
access: public

BBa_K1362445
type: Component
persistent identity: component2410820
display id: component2410820
version: 1
top level: BBa_K1362204
definition: MGSS
access: public

BBa_K1362424
type: Component
persistent identity: component2410843
display id: component2410843
version: 1
top level: BBa_K1362204
definition: BsaI ->
access: public

BBa_J04450
type: Component
persistent identity: component2410842
display id: component2410842
version: 1
top level: BBa_K1362204
definition: BBa_J04450
access: public

BBa_K1362447
type: Component
persistent identity: component2410824
display id: component2410824
version: 1
top level: BBa_K1362204
definition: <-BsaI
access: public

BBa_K1362453
type: Component
persistent identity: component2410823
display id: component2410823
version: 1
top level: BBa_K1362204
definition: TEV clv.
access: public

BBa_K1362445
type: SequenceAnnotation
persistent identity: annotation2410820
display id: annotation2410820
version: 1
top level: BBa_K1362204
location: https://synbiohub.org/public/igem/BBa_K1362204/annotation2410820/range2410820/1
component: BBa_K1362445

https://synbiohub.org/public/igem/BBa_K1362204/annotation2410820/range2410820/1
type: Range
persistent identity: range2410820
display id: range2410820
version: 1
top level: BBa_K1362204
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_K1362458
type: SequenceAnnotation
persistent identity: annotation2410821
display id: annotation2410821
version: 1
top level: BBa_K1362204
location: https://synbiohub.org/public/igem/BBa_K1362204/annotation2410821/range2410821/1
component: BBa_K1362458

https://synbiohub.org/public/igem/BBa_K1362204/annotation2410821/range2410821/1
type: Range
persistent identity: range2410821
display id: range2410821
version: 1
top level: BBa_K1362204
direction: direction/reverse
start: 13
end: 30
orientation: inline

BBa_K1362449
type: SequenceAnnotation
persistent identity: annotation2410822
display id: annotation2410822
version: 1
top level: BBa_K1362204
location: https://synbiohub.org/public/igem/BBa_K1362204/annotation2410822/range2410822/1
component: BBa_K1362449

https://synbiohub.org/public/igem/BBa_K1362204/annotation2410822/range2410822/1
type: Range
persistent identity: range2410822
display id: range2410822
version: 1
top level: BBa_K1362204
direction: direction/reverse
start: 31
end: 39
orientation: inline

BBa_K1362453
type: SequenceAnnotation
persistent identity: annotation2410823
display id: annotation2410823
version: 1
top level: BBa_K1362204
location: https://synbiohub.org/public/igem/BBa_K1362204/annotation2410823/range2410823/1
component: BBa_K1362453

https://synbiohub.org/public/igem/BBa_K1362204/annotation2410823/range2410823/1
type: Range
persistent identity: range2410823
display id: range2410823
version: 1
top level: BBa_K1362204
direction: direction/reverse
start: 40
end: 60
orientation: inline

BBa_K1362447
type: SequenceAnnotation
persistent identity: annotation2410824
display id: annotation2410824
version: 1
top level: BBa_K1362204
location: https://synbiohub.org/public/igem/BBa_K1362204/annotation2410824/range2410824/1
component: BBa_K1362447

https://synbiohub.org/public/igem/BBa_K1362204/annotation2410824/range2410824/1
type: Range
persistent identity: range2410824
display id: range2410824
version: 1
top level: BBa_K1362204
direction: direction/reverse
start: 61
end: 66
orientation: inline

BBa_J04450
type: SequenceAnnotation
persistent identity: annotation2410842
display id: annotation2410842
version: 1
top level: BBa_K1362204
location: https://synbiohub.org/public/igem/BBa_K1362204/annotation2410842/range2410842/1
component: BBa_J04450

https://synbiohub.org/public/igem/BBa_K1362204/annotation2410842/range2410842/1
type: Range
persistent identity: range2410842
display id: range2410842
version: 1
top level: BBa_K1362204
direction: direction/reverse
start: 67
end: 1135
orientation: inline

BBa_K1362424
type: SequenceAnnotation
persistent identity: annotation2410843
display id: annotation2410843
version: 1
top level: BBa_K1362204
location: https://synbiohub.org/public/igem/BBa_K1362204/annotation2410843/range2410843/1
component: BBa_K1362424

https://synbiohub.org/public/igem/BBa_K1362204/annotation2410843/range2410843/1
type: Range
persistent identity: range2410843
display id: range2410843
version: 1
top level: BBa_K1362204
direction: direction/reverse
start: 1136
end: 1142
orientation: inline

BBa_K1362459
type: SequenceAnnotation
persistent identity: annotation2410844
display id: annotation2410844
version: 1
top level: BBa_K1362204
location: https://synbiohub.org/public/igem/BBa_K1362204/annotation2410844/range2410844/1
component: BBa_K1362459

https://synbiohub.org/public/igem/BBa_K1362204/annotation2410844/range2410844/1
type: Range
persistent identity: range2410844
display id: range2410844
version: 1
top level: BBa_K1362204
direction: direction/reverse
start: 1143
end: 1154
orientation: inline

BBa_J70594
type: SequenceAnnotation
persistent identity: annotation2410846
display id: annotation2410846
version: 1
top level: BBa_K1362204
location: https://synbiohub.org/public/igem/BBa_K1362204/annotation2410846/range2410846/1
component: BBa_J70594

https://synbiohub.org/public/igem/BBa_K1362204/annotation2410846/range2410846/1
type: Range
persistent identity: range2410846
display id: range2410846
version: 1
top level: BBa_K1362204
direction: direction/reverse
start: 1155
end: 1160
orientation: inline

BBa_J04450
type: ComponentDefinition
persistent identity: BBa J04450
display id: BBa_J04450
version: 1
was generated by: iGEM to SBOL conversion
description: RFP Coding Device
created: 2005-06-08T11:00:00Z
modified: 2015-08-31T04:08:14Z
mutable provenance: Davidson Synth-Aces
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Contains an IPTG inducible promoter an RBS, RFP, and terminator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _16_
m user id: 0
owner id: 328
owning group id: 16
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Tamar Odle
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0034
component: BBa_R0010
component: BBa_B0012
component: BBa_B0010
component: BBa_E1010
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J04450_sequence/1

BBa_B0034
type: Component
persistent identity: component1509404
display id: component1509404
version: 1
top level: BBa_J04450
definition: BBa_B0034
access: public

BBa_R0010
type: Component
persistent identity: component1509394
display id: component1509394
version: 1
top level: BBa_J04450
definition: LacI
access: public

BBa_B0012
type: Component
persistent identity: component1509427
display id: component1509427
version: 1
top level: BBa_J04450
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1509417
display id: component1509417
version: 1
top level: BBa_J04450
definition: BBa_B0010
access: public

BBa_E1010
type: Component
persistent identity: component1509411
display id: component1509411
version: 1
top level: BBa_J04450
definition: mRFP1
access: public

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1509394
display id: annotation1509394
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509394/range1509394/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_J04450/annotation1509394/range1509394/1
type: Range
persistent identity: range1509394
display id: range1509394
version: 1
top level: BBa_J04450
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1509404
display id: annotation1509404
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509404/range1509404/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J04450/annotation1509404/range1509404/1
type: Range
persistent identity: range1509404
display id: range1509404
version: 1
top level: BBa_J04450
direction: direction/forward
start: 209
end: 220
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1509411
display id: annotation1509411
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509411/range1509411/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_J04450/annotation1509411/range1509411/1
type: Range
persistent identity: range1509411
display id: range1509411
version: 1
top level: BBa_J04450
direction: direction/forward
start: 227
end: 907
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1509417
display id: annotation1509417
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509417/range1509417/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J04450/annotation1509417/range1509417/1
type: Range
persistent identity: range1509417
display id: range1509417
version: 1
top level: BBa_J04450
direction: direction/forward
start: 941
end: 1020
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1509427
display id: annotation1509427
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509427/range1509427/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J04450/annotation1509427/range1509427/1
type: Range
persistent identity: range1509427
display id: range1509427
version: 1
top level: BBa_J04450
direction: direction/forward
start: 1029
end: 1069
orientation: inline

BsaI ->
type: ComponentDefinition
persistent identity: BBa K1362424
display id: BBa_K1362424
version: 1
was generated by: iGEM to SBOL conversion
description: BsaI restriction site for RFC[105] cloning
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:05Z
mutable provenance: This standard restriction site sequence is used as part of the iGEM team Heidelberg 2014's Intein Toolbox [[#References|[1]]]. All associated standard sequences can be reviewed in RFC[???]
owned by: james
owned by: myers
mutable description: This is the reverse complement of a BsaI restriction site headed by an Adenine as a spacer-base to separate the recognition sequence from the outward-lying cutting sequence. It was used by us for scarless golden-gate cloning to fuse inteins to other proteins and thereby implement a variety of possible port-translational modifications.
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 12377
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This part is only the sequence of a restriction site. It will not be sent in as physical DNA and was merely created to easily compose new parts in the RFC[???] standard. In the actual cloning process this sequence was and is recommended to be inserted with the according PCR primers.
star: false
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_K1362424_sequence/1

MGSS
type: ComponentDefinition
persistent identity: BBa K1362445
display id: BBa_K1362445
version: 1
was generated by: iGEM to SBOL conversion
description: Start codon and GSS spacer
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:06Z
mutable provenance: designed manually
owned by: james
owned by: myers
mutable description: This part codes for the protein sequence MGSS.
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 22951
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
sequence: https://synbiohub.org/public/igem/BBa_K1362445_sequence/1

<-BsaI
type: ComponentDefinition
persistent identity: BBa K1362447
display id: BBa_K1362447
version: 1
was generated by: iGEM to SBOL conversion
description: BsaI reverse restriction site for RFC[???] cloning (shortened by one C)
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:06Z
mutable provenance: BsaI recognition sequence from rebase
owned by: james
owned by: myers
mutable description: This Sequence starts with a part of the reverse-complemented BsaI recognition site. The missing C of the recognition site must be included in the downstream part. It contains a spacer adenine so that BsaI will cut the bottom strand directly upstream and the top strand 4 nucleotides upstream.
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 22951
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K1362447_sequence/1

SSG
type: ComponentDefinition
persistent identity: BBa K1362449
display id: BBa_K1362449
version: 1
was generated by: iGEM to SBOL conversion
description: SSG linker
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:06Z
mutable provenance: Sequence excerpt from the pET-28a-c(+) series of vectors.
owned by: james
owned by: myers
mutable description: This part codes for the amino acid sequence SSG.
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 22951
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
sequence: https://synbiohub.org/public/igem/BBa_K1362449_sequence/1

TEV clv.
type: ComponentDefinition
persistent identity: BBa K1362453
display id: BBa_K1362453
version: 1
was generated by: iGEM to SBOL conversion
description: TEV protease recognition/cleavage site
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:06Z
mutable provenance: Tobacco Etch Virus
owned by: james
owned by: myers
mutable description: This part codes for the amino acid sequence ENLYFQG. This site is recognized by TEV protease (catalytic domain of the Tobacco Etch Virus nuclear inclusion a (NIa) protein), which will cleave the Gln-Gly peptide bond [[{{PAGENAME}}:Design#References|[1]]]. The final four nucleotides of this sequence are GGGT, which will be the overhang produced wenn a reverse-facing BsaI site (<partinfo>BBa_K1362423</partinfo>, <partinfo>BBa_K1362427</partinfo>, <partinfo>BBa_K1362447</partinfo>) is directly following this part, as in the Sortase A circularization constructs (<partinfo>BBa_K1362202</partinfo>, <partinfo>BBa_K1362203</partinfo>, <partinfo>BBa_K1362204</partinfo>, <partinfo>BBa_K1362205</partinfo>).
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 22951
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
sequence: https://synbiohub.org/public/igem/BBa_K1362453_sequence/1

His6
type: ComponentDefinition
persistent identity: BBa K1362458
display id: BBa_K1362458
version: 1
was generated by: iGEM to SBOL conversion
description: Hexahistidine tag
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:06Z
owned by: james
owned by: myers
mutable description: This part codes for a hexahistidine tag, which can be used in protein purification by affinity chromatography.
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 22951
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_K1362458_sequence/1

SortA mock
type: ComponentDefinition
persistent identity: BBa K1362459
display id: BBa_K1362459
version: 1
was generated by: iGEM to SBOL conversion
description: Sortase A post-cleavage residual sequence (LPET)
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:06Z
mutable provenance: ''Staphylococcus aureus''
owned by: james
owned by: myers
mutable description: This part codes for the amino acid sequence LPET. This is the N-terminal portion of the Sortase A recognition sequence up to its cleavage site [[{{PAGENAME}}:Design#References|[1]]]. The first four nucleotides of this sequence are CTTC, which will be the overhang produced wenn a forward-facing BsaI site (<partinfo>BBa_K1362424</partinfo>, <partinfo>BBa_K1362425</partinfo>) is directly following this part, as in the Sortase A non-circularization constructs (<partinfo>BBa_K1362204</partinfo>, <partinfo>BBa_K1362205</partinfo>).
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 22951
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
sequence: https://synbiohub.org/public/igem/BBa_K1362459_sequence/1

BBa_J70594
type: ComponentDefinition
persistent identity: BBa J70594
display id: BBa_J70594
version: 1
was generated by: iGEM to SBOL conversion
description: RFC12 TAATAA Tail Domain
created: 2010-06-17T11:00:00Z
modified: 2015-05-08T01:08:25Z
mutable provenance: Common Knowledge
owned by: james
owned by: myers
mutable description: A RFC12 compatible part that simply codes for two stop codons. This part does not have any degradation tag.
discontinued: false
dominant: true
group u list: _41_
m user id: 0
owner id: 6384
owning group id: 41
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Made with synthetic oligos: 5' AATTC GCGGCGC T ACTAGT TAATAA GCTAGC A GCGGCCG CTGCA 3' 5' GCGGCCGCTGCTAGC TTATTA ACTAGTAGCGCCGC G 3' Note that both primers were ordered phosphorylated. An alternative is to phosphorylate the primers yourself with a kinase.
star: false
creator: Joseph Lynch
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_J70594_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2071257
display id: annotation2071257
version: 1
top level: BBa_J70594
location: https://synbiohub.org/public/igem/BBa_J70594/annotation2071257/range2071257/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_J70594/annotation2071257/range2071257/1
type: Range
persistent identity: range2071257
display id: range2071257
version: 1
top level: BBa_J70594
direction: direction/forward
start: 1
end: 5
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

https://synbiohub.org/public/igem/BBa_K1362004_sequence/1
type: Sequence
persistent identity: BBa K1362004 sequence
display id: BBa_K1362004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aataattttgtttaactttaagaaggagatactagatgggcagtagtcatcatcatcatcatcacagcagcggcgaaaacctgtacttccagggtagagaccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttataggtctcacttccggaaacctaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1362090_sequence/1
type: Sequence
persistent identity: BBa K1362090 sequence
display id: BBa_K1362090_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aataattttgtttaactttaagaaggaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1362204_sequence/1
type: Sequence
persistent identity: BBa K1362204 sequence
display id: BBa_K1362204_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgggcagtagtcatcatcatcatcatcacagcagcggcgaaaacctgtacttccagggtagagaccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttataggtctcacttccggaaacctaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1362424_sequence/1
type: Sequence
persistent identity: BBa K1362424 sequence
display id: BBa_K1362424_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggtctca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1362445_sequence/1
type: Sequence
persistent identity: BBa K1362445 sequence
display id: BBa_K1362445_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgggcagtagt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1362447_sequence/1
type: Sequence
persistent identity: BBa K1362447 sequence
display id: BBa_K1362447_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agagac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1362449_sequence/1
type: Sequence
persistent identity: BBa K1362449 sequence
display id: BBa_K1362449_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agcagcggc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1362453_sequence/1
type: Sequence
persistent identity: BBa K1362453 sequence
display id: BBa_K1362453_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaaaacctgtacttccagggt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1362458_sequence/1
type: Sequence
persistent identity: BBa K1362458 sequence
display id: BBa_K1362458_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: catcatcatcatcatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1362459_sequence/1
type: Sequence
persistent identity: BBa K1362459 sequence
display id: BBa_K1362459_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cttccggaaacc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J04450_sequence/1
type: Sequence
persistent identity: BBa J04450 sequence
display id: BBa_J04450_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J70594_sequence/1
type: Sequence
persistent identity: BBa J70594 sequence
display id: BBa_J70594_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z