BBa_K1362414
1
RFC105 A
N-terminal start overhang (T)(A)-(G)ATG=RBS+Start RFC[105] A
2014-10-06T11:00:00Z
2015-05-08T01:10:05Z
This standard overhang part was developed as part of the iGEM team Heidelberg 2014's Intein Toolbox [[#References[1]]]. All standard sequences can be reviewed in RFC[???] [[#References[2]]].
This is a standard overhang sequence for in-frame cloning of Proteins of Interest in front or behind an Intein. A detailed cloning strategy is found on the iGEM team Heidelberg 2014's [[http://2014.igem.org/Team:Heidelberg|wiki page]] as well as in RFC[???]. Specifically, this part contains the overhang A used to insert a protein behind any RFC[10] compatible RBS and in-frame with the start-codon. It lies within the four bases formed by the second last base of the XbaI/SpeI scar or the first base in front of start codon respectively and the start codon itself.
false
false
_1738_
0
12377
9
Not in stock
false
This part is only the sequence of a standard overhang. It will not be sent in as physical DNA and was merely created to easily compose new parts in the RFC[???] standard. In the actual cloning process this sequence was and is recommended to be inserted with the according PCR primers.
false
Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
BBa_K1362999
1
BBa_K1362999
iGEMHD tag
2014-10-07T11:00:00Z
2015-05-08T01:10:06Z
Directly synthesized as an oligo as backtranslated with EMBOSS[1].
==References==
1. Rice, P., Longden, I. & Bleasby, A. EMBOSS: the European Molecular Biology Open Software Suite. Trends Genet. 16, 276???7 (2000).
This Part adds ultimate coolness to you your part.
More seriously, it served us as a random sequence being a better overlap site for CPEC cloning than the RFC[10] suffix.
false
false
_1738_
0
22830
9
Not in stock
false
We aimed for a sequence without secondary structures, balanced GC content.
false
Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
annotation2400474
1
iGEMHD
range2400474
1
3
20
annotation2400473
1
stop
range2400473
1
21
23
annotation2400472
1
stop
range2400472
1
24
26
BBa_K1362090
1
T7 RBS
strong T7 RBS
2014-10-02T11:00:00Z
2015-05-08T01:10:04Z
synthesized as found in the T7 genome and several commercial expression plasmids.
RFC10 compatible strong RBS derived from the T7 phage gene 10a (major capsid protein)[1]. When assembled to a coding part with the A of the start codon being part of the XbaI site, the RBS will be shifted one bp downstream compared to the native sequence.
The sequence was successfully used by the iGEM team Heidelberg 2014 for the expression of many proteins in E.coli.
1. Olinss, P. & Rangwala, S. H. Derived from Bacteriophage T7 mRNA Acts ELS an Enhancer of Translation of the lac2 Gene in. 16973???16976 (1989).
false
false
_1738_
0
22830
9
It's complicated
false
The 18 bp including the XbaI that can be found upstream of the presumably important part of the RBS were included into the sequence just to make sure it works. However to fully comply with RFC10 a G was inserted behind the XbaI site.
false
Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
annotation2393796
1
Shine-Dalgarno
range2393796
1
21
28
annotation2393795
1
t7 RBS
range2393795
1
11
28
BBa_K1362432
1
LaLy
λ-Lysozyme (cds only)
2014-10-07T11:00:00Z
2015-05-08T01:10:05Z
???
???
false
false
_1738_
0
22920
9
Not in stock
false
???
false
Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
annotation2407303
1
LaLy
range2407303
1
1
471
BBa_K1362421
1
RFC105 Z
C-terminal stop overhang TAAT=STOP+1/3Stop RFC[105] overhang Z
2014-10-06T11:00:00Z
2015-05-08T01:10:05Z
This standard overhang part was developed as part of the iGEM team Heidelberg 2014's Intein Toolbox [[#References|[1]]]. All standard sequences can be reviewed in RFC[???] [[#References|[2]]].
This is a standard overhang sequence for in-frame cloning of Proteins of Interest in front or behind an Intein. A detailed cloning strategy is found on the iGEM team Heidelberg 2014's [http://2014.igem.org/Team:Heidelberg/parts wiki page] as well as in RFC[???]. Specifically, this part contains the overhang F used to insert the last part of an intein-fused protein directly in front of an RFC[10] double stop-codon. It lies within the first for bases of the stopstop sequence.
false
false
_1738_
0
12377
9
Not in stock
false
This part is only the sequence of a standard overhang. It will not be sent in as physical DNA and was merely created to easily compose new parts in the RFC[???] standard. In the actual cloning process this sequence was and is recommended to be inserted with the according PCR primers.
false
Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
annotation2402257
1
stop
range2402257
1
1
4
BBa_G0000
1
scar
SpeI/XbaI scar for RBS-CDS junctions
2007-07-22T11:00:00Z
2015-08-31T04:07:27Z
SpeI/XbaI scar
This is the sequence of the SpeI/XbaI scar for RBS-CDS junctions in BioBricks standard assembly.
false
true
_41_
0
126
162
Not in stock
false
This is a shorter scar to ensure proper spacing between the RBS and CDS.
false
Reshma Shetty
BBa_K1362011
1
BBa_K1362011
RBS + λ-Lysozyme
2014-10-07T11:00:00Z
2015-05-08T01:10:04Z
???
???
false
false
_1738_
0
22920
9
In stock
false
???
false
Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
component2430360
1
BBa_K1362999
component2430352
1
BBa_K1362414
component2430356
1
BBa_K1362421
component2430351
1
BBa_G0000
component2430354
1
BBa_K1362432
component2430350
1
BBa_K1362090
annotation2430360
1
BBa_K1362999
range2430360
1
514
539
annotation2430354
1
BBa_K1362432
range2430354
1
39
509
annotation2430350
1
BBa_K1362090
range2430350
1
1
29
annotation2430351
1
BBa_G0000
range2430351
1
30
35
annotation2430356
1
BBa_K1362421
range2430356
1
510
513
annotation2430352
1
BBa_K1362414
range2430352
1
36
38
BBa_K1362432_sequence
1
gtagaaatcaataatcaacgtaaggcgttcctcgatatgctggcgtggtcggagggaactgataacggacgtcagaaaaccagaaatcatggttatgacgtcattgtaggcggagagctatttactgattactccgatcaccctcgcaaacttgtcacgctaaacccaaaactcaaatcaacaggcgccggacgctaccagcttctttcccgttggtgggatgcctaccgcaagcagcttggcctgaaagacttctctccgaaaagtcaggacgctgtggcattgcagcagattaaggagcgtggcgctttacctatgattgatcgtggtgatatccgtcaggcaatcgaccgttgcagcaatatctgggcttcactgccgggcgctggttatggtcagttcgagcataaggctgacagcctgattgcaaaattcaaagaagcgggcggaacggtcagagagattgatgta
BBa_G0000_sequence
1
tactag
BBa_K1362414_sequence
1
atg
BBa_K1362011_sequence
1
aataattttgtttaactttaagaaggagatactagatggtagaaatcaataatcaacgtaaggcgttcctcgatatgctggcgtggtcggagggaactgataacggacgtcagaaaaccagaaatcatggttatgacgtcattgtaggcggagagctatttactgattactccgatcaccctcgcaaacttgtcacgctaaacccaaaactcaaatcaacaggcgccggacgctaccagcttctttcccgttggtgggatgcctaccgcaagcagcttggcctgaaagacttctctccgaaaagtcaggacgctgtggcattgcagcagattaaggagcgtggcgctttacctatgattgatcgtggtgatatccgtcaggcaatcgaccgttgcagcaatatctgggcttcactgccgggcgctggttatggtcagttcgagcataaggctgacagcctgattgcaaaattcaaagaagcgggcggaacggtcagagagattgatgtataataaatcggtgaaatgcacgactgatag
BBa_K1362999_sequence
1
aaatcggtgaaatgcacgactgatag
BBa_K1362421_sequence
1
taat
BBa_K1362090_sequence
1
aataattttgtttaactttaagaaggaga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z