LOCUS       BBa_K1362013            1016 bp    DNA     linear       22-Nov-2024
DEFINITION  RBS + Circular λ-Lysozyme with rigid linker
ACCESSION   BBa_K1362013
VERSION     BBa_K1362013.1  
FEATURES             Location/Qualifiers
     RBS             11..28
                     /label=t7 RBS
     misc_feature    21..28
                     /label=Shine-Dalgarno
     RBS             1..29
                     /label=BBa_K1362090
     misc_feature    30..35
                     /label=BBa_G0000
     misc_feature    36..38
                     /label=BBa_K1362414
     misc_feature    39..139
                     /label=BBa_K1362401
     misc_feature    140..143
                     /label=BBa_K1362419
     misc_feature    144..152
                     /label=CWE
     misc_feature    144..152
                     /label=BBa_K1362433
     CDS             153..623
                     /label=LaLy
     misc_feature    153..623
                     /label=BBa_K1362432
     misc_feature    624..671
                     /label=rigid linker
     misc_feature    624..671
                     /label=BBa_K1362439
     misc_feature    672..680
                     /label=RGK
     misc_feature    672..680
                     /label=BBa_K1362434
     misc_feature    681..684
                     /label=BBa_K1362415
     misc_feature    685..986
                     /label=BBa_K1362400
     misc_feature    987..990
                     /label=stop
     misc_feature    987..990
                     /label=BBa_K1362421
     misc_feature    1014..1016
                     /label=stop
     misc_feature    1011..1013
                     /label=stop
     misc_feature    993..1010
                     /label=iGEMHD
     misc_feature    991..1016
                     /label=BBa_K1362999
ORIGIN
        1 aataattttg tttaacttta agaaggagat actagatgat caaaatagcc acacgtaaat
       61 atttaggcaa acaaaatgtc tatgacattg gagttgagcg cgaccataat tttgcactca
      121 aaaatggctt catagcttcc aactgctggg aagtagaaat caataatcaa cgtaaggcgt
      181 tcctcgatat gctggcgtgg tcggagggaa ctgataacgg acgtcagaaa accagaaatc
      241 atggttatga cgtcattgta ggcggagagc tatttactga ttactccgat caccctcgca
      301 aacttgtcac gctaaaccca aaactcaaat caacaggcgc cggacgctac cagcttcttt
      361 cccgttggtg ggatgcctac cgcaagcagc ttggcctgaa agacttctct ccgaaaagtc
      421 aggacgctgt ggcattgcag cagattaagg agcgtggcgc tttacctatg attgatcgtg
      481 gtgatatccg tcaggcaatc gaccgttgca gcaatatctg ggcttcactg ccgggcgctg
      541 gttatggtca gttcgagcat aaggctgaca gcctgattgc aaaattcaaa gaagcgggcg
      601 gaacggtcag agagattgat gtaggtggcg cagaagcagc agcaaaagaa gcagcagcaa
      661 aagcagcacc gcgtggtaaa tgcttaagct atgaaacgga aatattgaca gtagaatatg
      721 gattattacc gattggtaaa attgtagaaa agcgcatcga atgtactgtt tatagcgttg
      781 ataataatgg aaatatttat acacaacctg tagcacaatg gcacgatcgc ggagaacaag
      841 aggtgtttga gtattgtttg gaagatggtt cattgattcg ggcaacaaaa gaccataagt
      901 ttatgactgt tgatggtcaa atgttgccaa ttgatgaaat atttgaacgt gaattggatt
      961 tgatgcgggt tgataatttg ccgaattaat aaatcggtga aatgcacgac tgatag
//