BBa_K1362022
type: ComponentDefinition
persistent identity: BBa K1362022
display id: BBa_K1362022
version: 1
was generated by: iGEM to SBOL conversion
description: RBS + Circular Xylanase
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:04Z
mutable provenance: ???
owned by: james
owned by: myers
mutable description: ???
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 22920
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: ???
star: false
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
component: BBa_K1362434
component: BBa_K1362441
component: BBa_K1362433
component: BBa_K1362419
component: BBa_K1362401
component: BBa_K1362414
component: BBa_G0000
component: BBa_K1362090
component: BBa_K1362999
component: BBa_K1362421
component: BBa_K1362400
component: BBa_K1362415
sequence annotation: BBa_K1362090
sequence annotation: BBa_G0000
sequence annotation: BBa_K1362414
sequence annotation: BBa_K1362401
sequence annotation: BBa_K1362419
sequence annotation: BBa_K1362433
sequence annotation: BBa_K1362441
sequence annotation: BBa_K1362434
sequence annotation: BBa_K1362415
sequence annotation: BBa_K1362400
sequence annotation: BBa_K1362421
sequence annotation: BBa_K1362999
sequence: https://synbiohub.org/public/igem/BBa_K1362022_sequence/1

BBa_K1362434
type: Component
persistent identity: component2409627
display id: component2409627
version: 1
top level: BBa_K1362022
definition: RGK
access: public

BBa_K1362441
type: Component
persistent identity: component2409625
display id: component2409625
version: 1
top level: BBa_K1362022
definition: Xyla
access: public

BBa_K1362433
type: Component
persistent identity: component2409623
display id: component2409623
version: 1
top level: BBa_K1362022
definition: CWE
access: public

BBa_K1362419
type: Component
persistent identity: component2409621
display id: component2409621
version: 1
top level: BBa_K1362022
definition: RFC105 CC
access: public

BBa_K1362401
type: Component
persistent identity: component2409620
display id: component2409620
version: 1
top level: BBa_K1362022
definition: NpuDnaE(C)
access: public

BBa_K1362414
type: Component
persistent identity: component2409619
display id: component2409619
version: 1
top level: BBa_K1362022
definition: RFC105 A
access: public

BBa_G0000
type: Component
persistent identity: component2409618
display id: component2409618
version: 1
top level: BBa_K1362022
definition: scar
access: public

BBa_K1362090
type: Component
persistent identity: component2409617
display id: component2409617
version: 1
top level: BBa_K1362022
definition: T7 RBS
access: public

BBa_K1362999
type: Component
persistent identity: component2409635
display id: component2409635
version: 1
top level: BBa_K1362022
definition: BBa_K1362999
access: public

BBa_K1362421
type: Component
persistent identity: component2409631
display id: component2409631
version: 1
top level: BBa_K1362022
definition: RFC105 Z
access: public

BBa_K1362400
type: Component
persistent identity: component2409629
display id: component2409629
version: 1
top level: BBa_K1362022
definition: NpuDnaE(N)
access: public

BBa_K1362415
type: Component
persistent identity: component2409628
display id: component2409628
version: 1
top level: BBa_K1362022
definition: RFC105 NN
access: public

BBa_K1362090
type: SequenceAnnotation
persistent identity: annotation2409617
display id: annotation2409617
version: 1
top level: BBa_K1362022
location: https://synbiohub.org/public/igem/BBa_K1362022/annotation2409617/range2409617/1
component: BBa_K1362090

https://synbiohub.org/public/igem/BBa_K1362022/annotation2409617/range2409617/1
type: Range
persistent identity: range2409617
display id: range2409617
version: 1
top level: BBa_K1362022
direction: direction/reverse
start: 1
end: 29
orientation: inline

BBa_G0000
type: SequenceAnnotation
persistent identity: annotation2409618
display id: annotation2409618
version: 1
top level: BBa_K1362022
location: https://synbiohub.org/public/igem/BBa_K1362022/annotation2409618/range2409618/1
component: BBa_G0000

https://synbiohub.org/public/igem/BBa_K1362022/annotation2409618/range2409618/1
type: Range
persistent identity: range2409618
display id: range2409618
version: 1
top level: BBa_K1362022
direction: direction/reverse
start: 30
end: 35
orientation: inline

BBa_K1362414
type: SequenceAnnotation
persistent identity: annotation2409619
display id: annotation2409619
version: 1
top level: BBa_K1362022
location: https://synbiohub.org/public/igem/BBa_K1362022/annotation2409619/range2409619/1
component: BBa_K1362414

https://synbiohub.org/public/igem/BBa_K1362022/annotation2409619/range2409619/1
type: Range
persistent identity: range2409619
display id: range2409619
version: 1
top level: BBa_K1362022
direction: direction/reverse
start: 36
end: 38
orientation: inline

BBa_K1362401
type: SequenceAnnotation
persistent identity: annotation2409620
display id: annotation2409620
version: 1
top level: BBa_K1362022
location: https://synbiohub.org/public/igem/BBa_K1362022/annotation2409620/range2409620/1
component: BBa_K1362401

https://synbiohub.org/public/igem/BBa_K1362022/annotation2409620/range2409620/1
type: Range
persistent identity: range2409620
display id: range2409620
version: 1
top level: BBa_K1362022
direction: direction/reverse
start: 39
end: 139
orientation: inline

BBa_K1362419
type: SequenceAnnotation
persistent identity: annotation2409621
display id: annotation2409621
version: 1
top level: BBa_K1362022
location: https://synbiohub.org/public/igem/BBa_K1362022/annotation2409621/range2409621/1
component: BBa_K1362419

https://synbiohub.org/public/igem/BBa_K1362022/annotation2409621/range2409621/1
type: Range
persistent identity: range2409621
display id: range2409621
version: 1
top level: BBa_K1362022
direction: direction/reverse
start: 140
end: 143
orientation: inline

BBa_K1362433
type: SequenceAnnotation
persistent identity: annotation2409623
display id: annotation2409623
version: 1
top level: BBa_K1362022
location: https://synbiohub.org/public/igem/BBa_K1362022/annotation2409623/range2409623/1
component: BBa_K1362433

https://synbiohub.org/public/igem/BBa_K1362022/annotation2409623/range2409623/1
type: Range
persistent identity: range2409623
display id: range2409623
version: 1
top level: BBa_K1362022
direction: direction/reverse
start: 144
end: 152
orientation: inline

BBa_K1362441
type: SequenceAnnotation
persistent identity: annotation2409625
display id: annotation2409625
version: 1
top level: BBa_K1362022
location: https://synbiohub.org/public/igem/BBa_K1362022/annotation2409625/range2409625/1
component: BBa_K1362441

https://synbiohub.org/public/igem/BBa_K1362022/annotation2409625/range2409625/1
type: Range
persistent identity: range2409625
display id: range2409625
version: 1
top level: BBa_K1362022
direction: direction/reverse
start: 153
end: 707
orientation: inline

BBa_K1362434
type: SequenceAnnotation
persistent identity: annotation2409627
display id: annotation2409627
version: 1
top level: BBa_K1362022
location: https://synbiohub.org/public/igem/BBa_K1362022/annotation2409627/range2409627/1
component: BBa_K1362434

https://synbiohub.org/public/igem/BBa_K1362022/annotation2409627/range2409627/1
type: Range
persistent identity: range2409627
display id: range2409627
version: 1
top level: BBa_K1362022
direction: direction/reverse
start: 708
end: 716
orientation: inline

BBa_K1362415
type: SequenceAnnotation
persistent identity: annotation2409628
display id: annotation2409628
version: 1
top level: BBa_K1362022
location: https://synbiohub.org/public/igem/BBa_K1362022/annotation2409628/range2409628/1
component: BBa_K1362415

https://synbiohub.org/public/igem/BBa_K1362022/annotation2409628/range2409628/1
type: Range
persistent identity: range2409628
display id: range2409628
version: 1
top level: BBa_K1362022
direction: direction/reverse
start: 717
end: 720
orientation: inline

BBa_K1362400
type: SequenceAnnotation
persistent identity: annotation2409629
display id: annotation2409629
version: 1
top level: BBa_K1362022
location: https://synbiohub.org/public/igem/BBa_K1362022/annotation2409629/range2409629/1
component: BBa_K1362400

https://synbiohub.org/public/igem/BBa_K1362022/annotation2409629/range2409629/1
type: Range
persistent identity: range2409629
display id: range2409629
version: 1
top level: BBa_K1362022
direction: direction/reverse
start: 721
end: 1022
orientation: inline

BBa_K1362421
type: SequenceAnnotation
persistent identity: annotation2409631
display id: annotation2409631
version: 1
top level: BBa_K1362022
location: https://synbiohub.org/public/igem/BBa_K1362022/annotation2409631/range2409631/1
component: BBa_K1362421

https://synbiohub.org/public/igem/BBa_K1362022/annotation2409631/range2409631/1
type: Range
persistent identity: range2409631
display id: range2409631
version: 1
top level: BBa_K1362022
direction: direction/reverse
start: 1023
end: 1026
orientation: inline

BBa_K1362999
type: SequenceAnnotation
persistent identity: annotation2409635
display id: annotation2409635
version: 1
top level: BBa_K1362022
location: https://synbiohub.org/public/igem/BBa_K1362022/annotation2409635/range2409635/1
component: BBa_K1362999

https://synbiohub.org/public/igem/BBa_K1362022/annotation2409635/range2409635/1
type: Range
persistent identity: range2409635
display id: range2409635
version: 1
top level: BBa_K1362022
direction: direction/reverse
start: 1027
end: 1052
orientation: inline

scar
type: ComponentDefinition
persistent identity: BBa G0000
display id: BBa_G0000
version: 1
was generated by: iGEM to SBOL conversion
description: SpeI/XbaI scar for RBS-CDS junctions
created: 2007-07-22T11:00:00Z
modified: 2015-08-31T04:07:27Z
mutable provenance: SpeI/XbaI scar
owned by: james
owned by: myers
mutable description: This is the sequence of the SpeI/XbaI scar for RBS-CDS junctions in BioBricks standard assembly.
discontinued: false
dominant: true
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This is a shorter scar to ensure proper spacing between the RBS and CDS.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_G0000_sequence/1

T7 RBS
type: ComponentDefinition
persistent identity: BBa K1362090
display id: BBa_K1362090
version: 1
was generated by: iGEM to SBOL conversion
description: strong T7 RBS
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:04Z
mutable provenance: synthesized as found in the T7 genome and several commercial expression plasmids.
owned by: james
owned by: myers
mutable description: RFC10 compatible strong RBS derived from the T7 phage gene 10a (major capsid protein)[1]. When assembled to a coding part with the A of the start codon being part of the XbaI site, the RBS will be shifted one bp downstream compared to the native sequence. The sequence was successfully used by the iGEM team Heidelberg 2014 for the expression of many proteins in E.coli. 1. Olinss, P. & Rangwala, S. H. Derived from Bacteriophage T7 mRNA Acts ELS an Enhancer of Translation of the lac2 Gene in. 16973???16976 (1989).
discontinued: false
dominant: false
experience: experience/Works
group u list: _1738_
m user id: 0
owner id: 22830
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: The 18 bp including the XbaI that can be found upstream of the presumably important part of the RBS were included into the sequence just to make sure it works. However to fully comply with RFC10 a G was inserted behind the XbaI site.
star: false
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: t7 RBS
sequence annotation: Shine-Dalgarno
sequence: https://synbiohub.org/public/igem/BBa_K1362090_sequence/1

t7 RBS
type: SequenceAnnotation
persistent identity: annotation2393795
display id: annotation2393795
version: 1
top level: T7 RBS
location: https://synbiohub.org/public/igem/BBa_K1362090/annotation2393795/range2393795/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K1362090/annotation2393795/range2393795/1
type: Range
persistent identity: range2393795
display id: range2393795
version: 1
top level: T7 RBS
direction: direction/forward
start: 11
end: 28
orientation: inline

Shine-Dalgarno
type: SequenceAnnotation
persistent identity: annotation2393796
display id: annotation2393796
version: 1
top level: T7 RBS
location: https://synbiohub.org/public/igem/BBa_K1362090/annotation2393796/range2393796/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K1362090/annotation2393796/range2393796/1
type: Range
persistent identity: range2393796
display id: range2393796
version: 1
top level: T7 RBS
direction: direction/forward
start: 21
end: 28
orientation: inline

RFC105 A
type: ComponentDefinition
persistent identity: BBa K1362414
display id: BBa_K1362414
version: 1
was generated by: iGEM to SBOL conversion
description: N-terminal start overhang (T)(A)-(G)ATG=RBS+Start RFC[105] A
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:05Z
mutable provenance: This standard overhang part was developed as part of the iGEM team Heidelberg 2014's Intein Toolbox [[#References[1]]]. All standard sequences can be reviewed in RFC[???] [[#References[2]]].
owned by: james
owned by: myers
mutable description: This is a standard overhang sequence for in-frame cloning of Proteins of Interest in front or behind an Intein. A detailed cloning strategy is found on the iGEM team Heidelberg 2014's wiki page as well as in RFC[???]. Specifically, this part contains the overhang A used to insert a protein behind any RFC[10] compatible RBS and in-frame with the start-codon. It lies within the four bases formed by the second last base of the XbaI/SpeI scar or the first base in front of start codon respectively and the start codon itself.
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 12377
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This part is only the sequence of a standard overhang. It will not be sent in as physical DNA and was merely created to easily compose new parts in the RFC[???] standard. In the actual cloning process this sequence was and is recommended to be inserted with the according PCR primers.
star: false
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K1362414_sequence/1

NpuDnaE(C)
type: ComponentDefinition
persistent identity: BBa K1362401
display id: BBa_K1362401
version: 1
was generated by: iGEM to SBOL conversion
description: NpuDnaE C-Intein cloning piece
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:05Z
mutable provenance: Obtained from pVS41 by Prof. Henning D. Mootz, University of Muenster.
owned by: james
owned by: myers
mutable description: Nostoc punctiforme DnaE split Intein C-terminal half. This is a DNA piece for cloning used to assemble our other BioBrick parts.
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 12377
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This part represents only the intein sequence without inluding the standard splicing-site or polyglycine-linker overhangs.
star: false
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K1362401_sequence/1

RFC105 CC
type: ComponentDefinition
persistent identity: BBa K1362419
display id: BBa_K1362419
version: 1
was generated by: iGEM to SBOL conversion
description: C-terminal splicing overhang CAAC=1/3?+Asn RFC[105] standard overhang CC
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:05Z
mutable provenance: This standard overhang part was developed as part of the iGEM team Heidelberg 2014's Intein Toolbox [[#References|[1]]]. All standard sequences can be reviewed in RFC[???] [[#References|[2]]].
owned by: james
owned by: myers
mutable description: This is a standard overhang sequence for in-frame cloning of Proteins of Interest in front or behind an Intein. A detailed cloning strategy is found on the iGEM team Heidelberg 2014's [http://2014.igem.org/Team:Heidelberg/parts wiki page] as well as in RFC[???]. Specifically, this part contains the overhang E used to insert a protein in front of an N-Intein. It lies within the four bases formed by the cystein and the first base of the subsequent Leucine/Isoleucine or similar of the N-terminal splicing site.
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 12377
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This part is only the sequence of a standard overhang. It will not be sent in as physical DNA and was merely created to easily compose new parts in the RFC[???] standard. In the actual cloning process this sequence was and is recommended to be inserted with the according PCR primers.
star: false
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
sequence: https://synbiohub.org/public/igem/BBa_K1362419_sequence/1

CWE
type: ComponentDefinition
persistent identity: BBa K1362433
display id: BBa_K1362433
version: 1
was generated by: iGEM to SBOL conversion
description: CWE (extein)
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:05Z
mutable provenance: ???
owned by: james
owned by: myers
mutable description: ???
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 22920
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: ???
star: false
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: CWE
sequence: https://synbiohub.org/public/igem/BBa_K1362433_sequence/1

CWE
type: SequenceAnnotation
persistent identity: annotation2407307
display id: annotation2407307
version: 1
top level: CWE
location: https://synbiohub.org/public/igem/BBa_K1362433/annotation2407307/range2407307/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K1362433/annotation2407307/range2407307/1
type: Range
persistent identity: range2407307
display id: range2407307
version: 1
top level: CWE
direction: direction/forward
start: 1
end: 9
orientation: inline

Xyla
type: ComponentDefinition
persistent identity: BBa K1362441
display id: BBa_K1362441
version: 1
was generated by: iGEM to SBOL conversion
description: Xylanase (cds only)
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:05Z
mutable provenance: ???
owned by: james
owned by: myers
mutable description: ???
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 22920
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: ???
star: false
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
sequence annotation: Xylanase
sequence: https://synbiohub.org/public/igem/BBa_K1362441_sequence/1

Xylanase
type: SequenceAnnotation
persistent identity: annotation2407315
display id: annotation2407315
version: 1
top level: Xyla
location: https://synbiohub.org/public/igem/BBa_K1362441/annotation2407315/range2407315/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K1362441/annotation2407315/range2407315/1
type: Range
persistent identity: range2407315
display id: range2407315
version: 1
top level: Xyla
direction: direction/forward
start: 1
end: 555
orientation: inline

NpuDnaE(N)
type: ComponentDefinition
persistent identity: BBa K1362400
display id: BBa_K1362400
version: 1
was generated by: iGEM to SBOL conversion
description: NpuDnaE N-Intein cloning piece
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:05Z
mutable provenance: Obtained from pVS07 by Prof. Henning D. Mootz, University of Muenster.
owned by: james
owned by: myers
mutable description: Nostoc punctiforme DnaE split Intein N-terminal half. This is a DNA piece for cloning used to assemble other BioBrick parts.
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 12377
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This part represents only the intein sequence without inluding the standard splicing-site or polyglycine-linker overhangs.
star: false
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
sequence: https://synbiohub.org/public/igem/BBa_K1362400_sequence/1

RFC105 NN
type: ComponentDefinition
persistent identity: BBa K1362415
display id: BBa_K1362415
version: 1
was generated by: iGEM to SBOL conversion
description: N-terminal splicing overhang TGCT=Cys+1/3 Leu|Ile RFC[105] overhang NN
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:05Z
mutable provenance: This standard overhang part was developed as part of the iGEM team Heidelberg 2014's Intein Toolbox [[#References[1]]]. All standard sequences can be reviewed in RFC[???] [[#References[2]]].
owned by: james
owned by: myers
mutable description: This is a standard overhang sequence for in-frame cloning of Proteins of Interest in front or behind an Intein. A detailed cloning strategy is found on the iGEM team Heidelberg 2014's wiki page as well as in RFC[???]. Specifically, this part contains the overhang A used to insert a protein behind any RFC[10] compatible RBS and in-frame with the start-codon. It lies within the four bases formed by the second last base of the XbaI/SpeI scar or the first base in front of start codon respectively and the start codon itself.
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 12377
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This part is only the sequence of a standard overhang. It will not be sent in as physical DNA and was merely created to easily compose new parts in the RFC[???] standard. In the actual cloning process this sequence was and is recommended to be inserted with the according PCR primers.
star: false
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K1362415_sequence/1

RFC105 Z
type: ComponentDefinition
persistent identity: BBa K1362421
display id: BBa_K1362421
version: 1
was generated by: iGEM to SBOL conversion
description: C-terminal stop overhang TAAT=STOP+1/3Stop RFC[105] overhang Z
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:05Z
mutable provenance: This standard overhang part was developed as part of the iGEM team Heidelberg 2014's Intein Toolbox [[#References|[1]]]. All standard sequences can be reviewed in RFC[???] [[#References|[2]]].
owned by: james
owned by: myers
mutable description: This is a standard overhang sequence for in-frame cloning of Proteins of Interest in front or behind an Intein. A detailed cloning strategy is found on the iGEM team Heidelberg 2014's [http://2014.igem.org/Team:Heidelberg/parts wiki page] as well as in RFC[???]. Specifically, this part contains the overhang F used to insert the last part of an intein-fused protein directly in front of an RFC[10] double stop-codon. It lies within the first for bases of the stopstop sequence.
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 12377
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This part is only the sequence of a standard overhang. It will not be sent in as physical DNA and was merely created to easily compose new parts in the RFC[???] standard. In the actual cloning process this sequence was and is recommended to be inserted with the according PCR primers.
star: false
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K1362421_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2402257
display id: annotation2402257
version: 1
top level: RFC105 Z
location: https://synbiohub.org/public/igem/BBa_K1362421/annotation2402257/range2402257/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K1362421/annotation2402257/range2402257/1
type: Range
persistent identity: range2402257
display id: range2402257
version: 1
top level: RFC105 Z
direction: direction/forward
start: 1
end: 4
orientation: inline

BBa_K1362999
type: ComponentDefinition
persistent identity: BBa K1362999
display id: BBa_K1362999
version: 1
was generated by: iGEM to SBOL conversion
description: iGEMHD tag
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:06Z
mutable provenance: Directly synthesized as an oligo as backtranslated with EMBOSS[1]. ==References== 1. Rice, P., Longden, I. & Bleasby, A. EMBOSS: the European Molecular Biology Open Software Suite. Trends Genet. 16, 276???7 (2000).
owned by: james
owned by: myers
mutable description: This Part adds ultimate coolness to you your part. More seriously, it served us as a random sequence being a better overlap site for CPEC cloning than the RFC[10] suffix.
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 22830
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: We aimed for a sequence without secondary structures, balanced GC content.
star: false
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
type: DnaRegion
role: partType/Tag
role: SO:0000324
sequence annotation: iGEMHD
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K1362999_sequence/1

iGEMHD
type: SequenceAnnotation
persistent identity: annotation2400474
display id: annotation2400474
version: 1
top level: BBa_K1362999
location: https://synbiohub.org/public/igem/BBa_K1362999/annotation2400474/range2400474/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_K1362999/annotation2400474/range2400474/1
type: Range
persistent identity: range2400474
display id: range2400474
version: 1
top level: BBa_K1362999
direction: direction/forward
start: 3
end: 20
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2400473
display id: annotation2400473
version: 1
top level: BBa_K1362999
location: https://synbiohub.org/public/igem/BBa_K1362999/annotation2400473/range2400473/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K1362999/annotation2400473/range2400473/1
type: Range
persistent identity: range2400473
display id: range2400473
version: 1
top level: BBa_K1362999
direction: direction/forward
start: 21
end: 23
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2400472
display id: annotation2400472
version: 1
top level: BBa_K1362999
location: https://synbiohub.org/public/igem/BBa_K1362999/annotation2400472/range2400472/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K1362999/annotation2400472/range2400472/1
type: Range
persistent identity: range2400472
display id: range2400472
version: 1
top level: BBa_K1362999
direction: direction/forward
start: 24
end: 26
orientation: inline

RGK
type: ComponentDefinition
persistent identity: BBa K1362434
display id: BBa_K1362434
version: 1
was generated by: iGEM to SBOL conversion
description: RGK (extein)
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:05Z
mutable provenance: ???
owned by: james
owned by: myers
mutable description: ???
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 22920
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: ???
star: false
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: RGK
sequence: https://synbiohub.org/public/igem/BBa_K1362434_sequence/1

RGK
type: SequenceAnnotation
persistent identity: annotation2407309
display id: annotation2407309
version: 1
top level: RGK
location: https://synbiohub.org/public/igem/BBa_K1362434/annotation2407309/range2407309/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1362434/annotation2407309/range2407309/1
type: Range
persistent identity: range2407309
display id: range2407309
version: 1
top level: RGK
direction: direction/forward
start: 1
end: 9
orientation: inline

https://synbiohub.org/public/igem/BBa_K1362022_sequence/1
type: Sequence
persistent identity: BBa K1362022 sequence
display id: BBa_K1362022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aataattttgtttaactttaagaaggagatactagatgatcaaaatagccacacgtaaatatttaggcaaacaaaatgtctatgacattggagttgagcgcgaccataattttgcactcaaaaatggcttcatagcttccaactgctgggaagctagcacagactactggcaaaattggactgatgggggcggtatagtaaacgctgtcaatgggtctggcgggaattacagtgttaattggtctaataccggaaattttgttgttggtaaaggttggactacaggttcgccatttaggacgataaactataatgccggagtttgggcgccgaatggcaatggatatttaactttatatggttggacgagatcacctctcatagaatattatgtagtggattcatggggtacttatagacctactggaacgtataaaggtactgtaaaaagtgatgggggtacatatgacatatatacaactacacgttataacgcaccttccattgatggcgatcgcactacttttacgcagtactggagtgttcgccagtcgaagagaccaaccggaagcaacgctacaatcactttcagcaatcatgtgaacgcatggaagagccatggaatgaatctgggcagtaattgggcttaccaagtcatggcgacagaaggatatcaaagtagtggaagttctaacgtaacagtgtggcgtggtaaatgcttaagctatgaaacggaaatattgacagtagaatatggattattaccgattggtaaaattgtagaaaagcgcatcgaatgtactgtttatagcgttgataataatggaaatatttatacacaacctgtagcacaatggcacgatcgcggagaacaagaggtgtttgagtattgtttggaagatggttcattgattcgggcaacaaaagaccataagtttatgactgttgatggtcaaatgttgccaattgatgaaatatttgaacgtgaattggatttgatgcgggttgataatttgccgaattaataaatcggtgaaatgcacgactgatag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G0000_sequence/1
type: Sequence
persistent identity: BBa G0000 sequence
display id: BBa_G0000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1362090_sequence/1
type: Sequence
persistent identity: BBa K1362090 sequence
display id: BBa_K1362090_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aataattttgtttaactttaagaaggaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1362414_sequence/1
type: Sequence
persistent identity: BBa K1362414 sequence
display id: BBa_K1362414_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1362401_sequence/1
type: Sequence
persistent identity: BBa K1362401 sequence
display id: BBa_K1362401_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atcaaaatagccacacgtaaatatttaggcaaacaaaatgtctatgacattggagttgagcgcgaccataattttgcactcaaaaatggcttcatagcttc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1362419_sequence/1
type: Sequence
persistent identity: BBa K1362419 sequence
display id: BBa_K1362419_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1362400_sequence/1
type: Sequence
persistent identity: BBa K1362400 sequence
display id: BBa_K1362400_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taagctatgaaacggaaatattgacagtagaatatggattattaccgattggtaaaattgtagaaaagcgcatcgaatgtactgtttatagcgttgataataatggaaatatttatacacaacctgtagcacaatggcacgatcgcggagaacaagaggtgtttgagtattgtttggaagatggttcattgattcgggcaacaaaagaccataagtttatgactgttgatggtcaaatgttgccaattgatgaaatatttgaacgtgaattggatttgatgcgggttgataatttgccgaat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1362415_sequence/1
type: Sequence
persistent identity: BBa K1362415 sequence
display id: BBa_K1362415_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1362421_sequence/1
type: Sequence
persistent identity: BBa K1362421 sequence
display id: BBa_K1362421_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1362999_sequence/1
type: Sequence
persistent identity: BBa K1362999 sequence
display id: BBa_K1362999_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaatcggtgaaatgcacgactgatag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1362433_sequence/1
type: Sequence
persistent identity: BBa K1362433 sequence
display id: BBa_K1362433_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgctgggaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1362434_sequence/1
type: Sequence
persistent identity: BBa K1362434 sequence
display id: BBa_K1362434_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgtggtaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1362441_sequence/1
type: Sequence
persistent identity: BBa K1362441 sequence
display id: BBa_K1362441_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctagcacagactactggcaaaattggactgatgggggcggtatagtaaacgctgtcaatgggtctggcgggaattacagtgttaattggtctaataccggaaattttgttgttggtaaaggttggactacaggttcgccatttaggacgataaactataatgccggagtttgggcgccgaatggcaatggatatttaactttatatggttggacgagatcacctctcatagaatattatgtagtggattcatggggtacttatagacctactggaacgtataaaggtactgtaaaaagtgatgggggtacatatgacatatatacaactacacgttataacgcaccttccattgatggcgatcgcactacttttacgcagtactggagtgttcgccagtcgaagagaccaaccggaagcaacgctacaatcactttcagcaatcatgtgaacgcatggaagagccatggaatgaatctgggcagtaattgggcttaccaagtcatggcgacagaaggatatcaaagtagtggaagttctaacgtaacagtgtgg
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z