LOCUS       BBa_K1362051            1717 bp    DNA     linear       04-May-2024
DEFINITION  Intein protease with arabinose inducible regulatory promoter/ 
            repressor araC-Pbad-T7RBS-His-SspDnaBC
ACCESSION   BBa_K1362051
VERSION     BBa_K1362051.1  
FEATURES             Location/Qualifiers
     CDS             1..879
                     /label=AraC
     misc_feature    909..924
                     /label=AraO
     misc_feature    1066..1077
                     /label=AraO1
     promoter        1153..1181
                     /label=AraPromoter
     promoter        1..1209
                     /label=BBa_K808000
     RBS             1228..1245
                     /label=t7 RBS
     misc_feature    1238..1245
                     /label=Shine-Dalgarno
     RBS             1218..1246
                     /label=BBa_K1362090
     misc_feature    1253..1255
                     /label=start
     misc_feature    1715..1717
                     /label=stop
     misc_feature    1712..1714
                     /label=stop
     misc_feature    1256..1273
                     /label=tag
     misc_feature    1274..1702
                     /label=SspDnaB C intein
     misc_feature    1703..1711
                     /label=SspDnaB C extein
     misc_feature    1700..1702
                     /label=N -> A
     CDS             1253..1717
                     /label=BBa_K1362251
     misc_feature    1218..1717
                     /label=BBa_K1362050
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 tgggctaact actagagaat aattttgttt aactttaaga aggagatact agatgcatca
     1261 tcaccatcac cacagcacag gaaaaagagt ttctattaaa gatttgttag atgaaaaaga
     1321 ttttgaaata tgggcaatta atgaacagac gatgaagcta gaatcagcta aagttagtcg
     1381 tgtattttgt actggcaaaa agctagttta tattctaaaa actcgactag gtagaactat
     1441 caaggcaaca gcaaatcata gatttttaac tattgatggt tggaaaagat tagatgagct
     1501 atctttaaaa gagcatattg ctctaccccg taaactagaa agctcctctt tacaattgtc
     1561 accagaaata gaaaagttgt ctcagagtga tatttactgg gactccatcg tttctattac
     1621 ggagactgga gtcgaagagg tttttgattt gactgtgcca ggaccacata actttgtcgc
     1681 caatgacatc attgtccatg cctcaggcgg gtaataa
//