BBa_K1362425 1 BsaI -> BsaI restriction site for RFC[???] cloning (in part in prefix) 2014-10-07T11:00:00Z 2015-05-08T01:10:05Z This Sequence starts with a part of the BsaI recognition site. The missing G of the recognition site can be found in the upstream BB prefix. It contains a spacer nucleotide so that BsaI will cut the top strand directly downstream and the bottom strand 4 nucleotides downstream. false false _1738_ 0 22830 9 Not in stock false false Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B&uuml;scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch&auml;fer, Carolin Schmelas, Silvan Schmitz, Max Waldha annotation2402282 1 RFC[???] standard overhang A range2402282 1 5 5 annotation2400588 1 BsaI site range2400588 1 1 5 BBa_K1362421 1 RFC105 Z C-terminal stop overhang TAAT=STOP+1/3Stop RFC[105] overhang Z 2014-10-06T11:00:00Z 2015-05-08T01:10:05Z This standard overhang part was developed as part of the iGEM team Heidelberg 2014's Intein Toolbox [[#References|[1]]]. All standard sequences can be reviewed in RFC[???] [[#References|[2]]]. This is a standard overhang sequence for in-frame cloning of Proteins of Interest in front or behind an Intein. A detailed cloning strategy is found on the iGEM team Heidelberg 2014's [http://2014.igem.org/Team:Heidelberg/parts wiki page] as well as in RFC[???]. Specifically, this part contains the overhang F used to insert the last part of an intein-fused protein directly in front of an RFC[10] double stop-codon. It lies within the first for bases of the stopstop sequence. false false _1738_ 0 12377 9 Not in stock false This part is only the sequence of a standard overhang. It will not be sent in as physical DNA and was merely created to easily compose new parts in the RFC[???] standard. In the actual cloning process this sequence was and is recommended to be inserted with the according PCR primers. false Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha annotation2402257 1 stop range2402257 1 1 4 BBa_K1362436 1 spacer Random spacer sequence 2014-10-07T11:00:00Z 2015-05-08T01:10:05Z /dev/random Random spacer sequence to ensure efficient cutting up and downstream. false false _1738_ 0 22830 9 Not in stock false false Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B&uuml;scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch&auml;fer, Carolin Schmelas, Silvan Schmitz, Max Waldha BBa_K1362426 1 spacer Random sequence 2014-10-07T11:00:00Z 2015-05-08T01:10:05Z It was suddenly there. This sequence is of no use. false false _1738_ 0 22830 9 Not in stock false No consideration was performed by the designer. false Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B&uuml;scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch&auml;fer, Carolin Schmelas, Silvan Schmitz, Max Waldha BBa_K1362419 1 RFC105 CC C-terminal splicing overhang CAAC=1/3?+Asn RFC[105] standard overhang CC 2014-10-06T11:00:00Z 2015-05-08T01:10:05Z This standard overhang part was developed as part of the iGEM team Heidelberg 2014's Intein Toolbox [[#References|[1]]]. All standard sequences can be reviewed in RFC[???] [[#References|[2]]]. This is a standard overhang sequence for in-frame cloning of Proteins of Interest in front or behind an Intein. A detailed cloning strategy is found on the iGEM team Heidelberg 2014's [http://2014.igem.org/Team:Heidelberg/parts wiki page] as well as in RFC[???]. Specifically, this part contains the overhang E used to insert a protein in front of an N-Intein. It lies within the four bases formed by the cystein and the first base of the subsequent Leucine/Isoleucine or similar of the N-terminal splicing site. false false _1738_ 0 12377 9 Not in stock false This part is only the sequence of a standard overhang. It will not be sent in as physical DNA and was merely created to easily compose new parts in the RFC[???] standard. In the actual cloning process this sequence was and is recommended to be inserted with the according PCR primers. false Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha BBa_K1362052 1 BBa_K1362052 Carrier for RFC[105] C-terminal inserts 2014-10-07T11:00:00Z 2015-05-08T01:10:04Z false false _1738_ 0 22911 9 In stock false false Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha component2409680 1 BBa_K1362421 component2409676 1 BBa_K1362425 component2409677 1 BBa_K1362419 component2409681 1 BBa_K1362423 component2409678 1 BBa_K1362436 component2409682 1 BBa_K1362426 annotation2409678 1 BBa_K1362436 range2409678 1 11 16 annotation2409681 1 BBa_K1362423 range2409681 1 21 27 annotation2409682 1 BBa_K1362426 range2409682 1 28 31 annotation2409680 1 BBa_K1362421 range2409680 1 17 20 annotation2409677 1 BBa_K1362419 range2409677 1 7 10 annotation2409676 1 BBa_K1362425 range2409676 1 1 6 BBa_K1362423 1 <- BsaI BsaI reverse restriction site for RFC[105] cloning 2014-10-06T11:00:00Z 2015-05-08T01:10:05Z This standard restriction site sequence is used as part of the iGEM team Heidelberg 2014's Intein Toolbox [[#References|[1]]]. All associated standard sequences can be reviewed in RFC[???] This is the reverse complement of a BsaI restriction site headed by an Adenine as a spacer-base to separate the recognition sequence from the outward-lying cutting sequence. It was used by us for scarless golden-gate cloning to fuse inteins to other proteins and thereby implement a variety of possible port-translational modifications. false false _1738_ 0 12377 9 Not in stock false This part is only the sequence of a restriction site. It will not be sent in as physical DNA and was merely created to easily compose new parts in the RFC[???] standard. In the actual cloning process this sequence was and is recommended to be inserted with the according PCR primers. false Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha BBa_K1362425_sequence 1 gtctcc BBa_K1362426_sequence 1 tgtc BBa_K1362419_sequence 1 caac BBa_K1362423_sequence 1 agagacc BBa_K1362052_sequence 1 gtctcccaaccctgcctaatagagacctgtc BBa_K1362436_sequence 1 cctgcc BBa_K1362421_sequence 1 taat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z