BBa_K1362440
1
RFC105 A
N-terminal start overhang (T)(A)-GATG=RBS+Start RFC[105] A
2014-10-07T11:00:00Z
2015-05-08T01:10:05Z
false
false
_1738_
0
22830
9
Not in stock
false
false
Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
BBa_K1362436
1
spacer
Random spacer sequence
2014-10-07T11:00:00Z
2015-05-08T01:10:05Z
/dev/random
Random spacer sequence to ensure efficient cutting up and downstream.
false
false
_1738_
0
22830
9
Not in stock
false
false
Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
BBa_K1362426
1
spacer
Random sequence
2014-10-07T11:00:00Z
2015-05-08T01:10:05Z
It was suddenly there.
This sequence is of no use.
false
false
_1738_
0
22830
9
Not in stock
false
No consideration was performed by the designer.
false
Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
BBa_K1362053
1
N-carrier
Carrier for RFC[105] N-terminal inserts
2014-10-07T11:00:00Z
2015-05-08T01:10:04Z
false
false
_1738_
0
22911
9
In stock
false
false
Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
component2409689
1
BBa_K1362423
component2409686
1
BBa_K1362440
component2409687
1
BBa_K1362436
component2409685
1
BBa_K1362425
component2409690
1
BBa_K1362426
component2409688
1
BBa_K1362415
annotation2409688
1
BBa_K1362415
range2409688
1
17
20
annotation2409685
1
BBa_K1362425
range2409685
1
1
6
annotation2409689
1
BBa_K1362423
range2409689
1
21
27
annotation2409687
1
BBa_K1362436
range2409687
1
11
16
annotation2409690
1
BBa_K1362426
range2409690
1
28
31
annotation2409686
1
BBa_K1362440
range2409686
1
7
10
BBa_K1362415
1
RFC105 NN
N-terminal splicing overhang TGCT=Cys+1/3 Leu|Ile RFC[105] overhang NN
2014-10-06T11:00:00Z
2015-05-08T01:10:05Z
This standard overhang part was developed as part of the iGEM team Heidelberg 2014's Intein Toolbox [[#References[1]]]. All standard sequences can be reviewed in RFC[???] [[#References[2]]].
This is a standard overhang sequence for in-frame cloning of Proteins of Interest in front or behind an Intein. A detailed cloning strategy is found on the iGEM team Heidelberg 2014's [[http://2014.igem.org/Team:Heidelberg|wiki page]] as well as in RFC[???]. Specifically, this part contains the overhang A used to insert a protein behind any RFC[10] compatible RBS and in-frame with the start-codon. It lies within the four bases formed by the second last base of the XbaI/SpeI scar or the first base in front of start codon respectively and the start codon itself.
false
false
_1738_
0
12377
9
Not in stock
false
This part is only the sequence of a standard overhang. It will not be sent in as physical DNA and was merely created to easily compose new parts in the RFC[???] standard. In the actual cloning process this sequence was and is recommended to be inserted with the according PCR primers.
false
Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
BBa_K1362425
1
BsaI ->
BsaI restriction site for RFC[???] cloning (in part in prefix)
2014-10-07T11:00:00Z
2015-05-08T01:10:05Z
This Sequence starts with a part of the BsaI recognition site. The missing G of the recognition site can be found in the upstream BB prefix. It contains a spacer nucleotide so that BsaI will cut the top strand directly downstream and the bottom strand 4 nucleotides downstream.
false
false
_1738_
0
22830
9
Not in stock
false
false
Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
annotation2402282
1
RFC[???] standard overhang A
range2402282
1
5
5
annotation2400588
1
BsaI site
range2400588
1
1
5
BBa_K1362423
1
<- BsaI
BsaI reverse restriction site for RFC[105] cloning
2014-10-06T11:00:00Z
2015-05-08T01:10:05Z
This standard restriction site sequence is used as part of the iGEM team Heidelberg 2014's Intein Toolbox [[#References|[1]]]. All associated standard sequences can be reviewed in RFC[???]
This is the reverse complement of a BsaI restriction site headed by an Adenine as a spacer-base to separate the recognition sequence from the outward-lying cutting sequence. It was used by us for scarless golden-gate cloning to fuse inteins to other proteins and thereby implement a variety of possible port-translational modifications.
false
false
_1738_
0
12377
9
Not in stock
false
This part is only the sequence of a restriction site. It will not be sent in as physical DNA and was merely created to easily compose new parts in the RFC[???] standard. In the actual cloning process this sequence was and is recommended to be inserted with the according PCR primers.
false
Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
BBa_K1362425_sequence
1
gtctcc
BBa_K1362426_sequence
1
tgtc
BBa_K1362423_sequence
1
agagacc
BBa_K1362440_sequence
1
gatg
BBa_K1362415_sequence
1
tgct
BBa_K1362053_sequence
1
gtctccgatgcctgcctgctagagacctgtc
BBa_K1362436_sequence
1
cctgcc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z