BBa_K1362425 1 BsaI -> BsaI restriction site for RFC[???] cloning (in part in prefix) 2014-10-07T11:00:00Z 2015-05-08T01:10:05Z This Sequence starts with a part of the BsaI recognition site. The missing G of the recognition site can be found in the upstream BB prefix. It contains a spacer nucleotide so that BsaI will cut the top strand directly downstream and the bottom strand 4 nucleotides downstream. false false _1738_ 0 22830 9 Not in stock false false Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B&uuml;scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch&auml;fer, Carolin Schmelas, Silvan Schmitz, Max Waldha annotation2402282 1 RFC[???] standard overhang A range2402282 1 5 5 annotation2400588 1 BsaI site range2400588 1 1 5 BBa_J32015 1 PelB PelB leader sequence; directs protein to E. coli periplasmic membrane 2006-08-24T11:00:00Z 2015-08-31T04:08:46Z Novagen Released HQ 2013 The pelB leader sequence is a sequence of amino acids which when attached to a protein, directs the protein to the periplasmic membrane of E. coli, where the sequence is removed by pelB peptidase. It is used to direct coat protein-antigen fusions to the cell surface. false true _50_ 0 495 50 In stock true . true Austen Heinz annotation1898182 1 PelB range1898182 1 1 66 BBa_K1362058 1 BBa_K1362058 PelB leading sequence as RFC[105] N-terminal insert 2014-10-07T11:00:00Z 2015-05-08T01:10:04Z false false _1738_ 0 22830 9 In stock false false Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha component2409735 1 BBa_J32015 component2409733 1 BBa_K1362425 component2409738 1 BBa_K1362423 component2409737 1 BBa_K1362415 component2409736 1 BBa_K1362444 annotation2409737 1 BBa_K1362415 range2409737 1 92 95 annotation2409738 1 BBa_K1362423 range2409738 1 96 102 annotation2409735 1 BBa_J32015 range2409735 1 8 73 annotation2409733 1 BBa_K1362425 range2409733 1 1 6 annotation2409736 1 BBa_K1362444 range2409736 1 74 91 BBa_K1362415 1 RFC105 NN N-terminal splicing overhang TGCT=Cys+1/3 Leu|Ile RFC[105] overhang NN 2014-10-06T11:00:00Z 2015-05-08T01:10:05Z This standard overhang part was developed as part of the iGEM team Heidelberg 2014's Intein Toolbox [[#References[1]]]. All standard sequences can be reviewed in RFC[???] [[#References[2]]]. This is a standard overhang sequence for in-frame cloning of Proteins of Interest in front or behind an Intein. A detailed cloning strategy is found on the iGEM team Heidelberg 2014's [[http://2014.igem.org/Team:Heidelberg|wiki page]] as well as in RFC[???]. Specifically, this part contains the overhang A used to insert a protein behind any RFC[10] compatible RBS and in-frame with the start-codon. It lies within the four bases formed by the second last base of the XbaI/SpeI scar or the first base in front of start codon respectively and the start codon itself. false false _1738_ 0 12377 9 Not in stock false This part is only the sequence of a standard overhang. It will not be sent in as physical DNA and was merely created to easily compose new parts in the RFC[???] standard. In the actual cloning process this sequence was and is recommended to be inserted with the according PCR primers. false Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha BBa_K1362423 1 <- BsaI BsaI reverse restriction site for RFC[105] cloning 2014-10-06T11:00:00Z 2015-05-08T01:10:05Z This standard restriction site sequence is used as part of the iGEM team Heidelberg 2014's Intein Toolbox [[#References|[1]]]. All associated standard sequences can be reviewed in RFC[???] This is the reverse complement of a BsaI restriction site headed by an Adenine as a spacer-base to separate the recognition sequence from the outward-lying cutting sequence. It was used by us for scarless golden-gate cloning to fuse inteins to other proteins and thereby implement a variety of possible port-translational modifications. false false _1738_ 0 12377 9 Not in stock false This part is only the sequence of a restriction site. It will not be sent in as physical DNA and was merely created to easily compose new parts in the RFC[???] standard. In the actual cloning process this sequence was and is recommended to be inserted with the according PCR primers. false Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha BBa_K1362444 1 BBa_K1362444 GGG linker with RGK (extein) 2014-10-07T11:00:00Z 2015-05-08T01:10:06Z false false _1738_ 0 22830 9 Not in stock false false Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B&uuml;scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch&auml;fer, Carolin Schmelas, Silvan Schmitz, Max Waldha BBa_K1362425_sequence 1 gtctcc BBa_K1362058_sequence 1 gtctccgatgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggccggcggaggcaggggcaagtgctagagacc BBa_K1362423_sequence 1 agagacc BBa_K1362415_sequence 1 tgct BBa_K1362444_sequence 1 ggcggaggcaggggcaag BBa_J32015_sequence 1 atgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggcc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z