BBa_K1362440
1
RFC105 A
N-terminal start overhang (T)(A)-GATG=RBS+Start RFC[105] A
2014-10-07T11:00:00Z
2015-05-08T01:10:05Z
false
false
_1738_
0
22830
9
Not in stock
false
false
Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
BBa_K1362425
1
BsaI ->
BsaI restriction site for RFC[???] cloning (in part in prefix)
2014-10-07T11:00:00Z
2015-05-08T01:10:05Z
This Sequence starts with a part of the BsaI recognition site. The missing G of the recognition site can be found in the upstream BB prefix. It contains a spacer nucleotide so that BsaI will cut the top strand directly downstream and the bottom strand 4 nucleotides downstream.
false
false
_1738_
0
22830
9
Not in stock
false
false
Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
annotation2402282
1
RFC[???] standard overhang A
range2402282
1
5
5
annotation2400588
1
BsaI site
range2400588
1
1
5
BBa_K1362059
1
BBa_K1362059
PKI-NES as RFC[105] N-terminal insert
2014-10-07T11:00:00Z
2015-05-08T01:10:04Z
false
false
_1738_
0
22830
9
In stock
false
false
Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
component2409744
1
BBa_K1362444
component2409743
1
BBa_J63007
component2409741
1
BBa_K1362425
component2409746
1
BBa_K1362423
component2409742
1
BBa_K1362440
component2409745
1
BBa_K1362415
annotation2409745
1
BBa_K1362415
range2409745
1
59
62
annotation2409741
1
BBa_K1362425
range2409741
1
1
6
annotation2409744
1
BBa_K1362444
range2409744
1
41
58
annotation2409742
1
BBa_K1362440
range2409742
1
7
10
annotation2409746
1
BBa_K1362423
range2409746
1
63
69
annotation2409743
1
BBa_J63007
range2409743
1
11
40
BBa_J63007
1
PKI NES
PKI nuclear export sequence; yeast codon optimized
2006-10-10T11:00:00Z
2015-08-31T01:56:26Z
from genomic DNA
yeast codon optimized PKI nuclear export sequence
false
false
_97_
0
545
97
It's complicated
false
optimized codons for S. cerevisiae
false
Caroline Ajo-Franklin
BBa_K1362415
1
RFC105 NN
N-terminal splicing overhang TGCT=Cys+1/3 Leu|Ile RFC[105] overhang NN
2014-10-06T11:00:00Z
2015-05-08T01:10:05Z
This standard overhang part was developed as part of the iGEM team Heidelberg 2014's Intein Toolbox [[#References[1]]]. All standard sequences can be reviewed in RFC[???] [[#References[2]]].
This is a standard overhang sequence for in-frame cloning of Proteins of Interest in front or behind an Intein. A detailed cloning strategy is found on the iGEM team Heidelberg 2014's [[http://2014.igem.org/Team:Heidelberg|wiki page]] as well as in RFC[???]. Specifically, this part contains the overhang A used to insert a protein behind any RFC[10] compatible RBS and in-frame with the start-codon. It lies within the four bases formed by the second last base of the XbaI/SpeI scar or the first base in front of start codon respectively and the start codon itself.
false
false
_1738_
0
12377
9
Not in stock
false
This part is only the sequence of a standard overhang. It will not be sent in as physical DNA and was merely created to easily compose new parts in the RFC[???] standard. In the actual cloning process this sequence was and is recommended to be inserted with the according PCR primers.
false
Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
BBa_K1362423
1
<- BsaI
BsaI reverse restriction site for RFC[105] cloning
2014-10-06T11:00:00Z
2015-05-08T01:10:05Z
This standard restriction site sequence is used as part of the iGEM team Heidelberg 2014's Intein Toolbox [[#References|[1]]]. All associated standard sequences can be reviewed in RFC[???]
This is the reverse complement of a BsaI restriction site headed by an Adenine as a spacer-base to separate the recognition sequence from the outward-lying cutting sequence. It was used by us for scarless golden-gate cloning to fuse inteins to other proteins and thereby implement a variety of possible port-translational modifications.
false
false
_1738_
0
12377
9
Not in stock
false
This part is only the sequence of a restriction site. It will not be sent in as physical DNA and was merely created to easily compose new parts in the RFC[???] standard. In the actual cloning process this sequence was and is recommended to be inserted with the according PCR primers.
false
Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
BBa_K1362444
1
BBa_K1362444
GGG linker with RGK (extein)
2014-10-07T11:00:00Z
2015-05-08T01:10:06Z
false
false
_1738_
0
22830
9
Not in stock
false
false
Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
BBa_K1362425_sequence
1
gtctcc
BBa_K1362423_sequence
1
agagacc
BBa_K1362440_sequence
1
gatg
BBa_K1362415_sequence
1
tgct
BBa_K1362444_sequence
1
ggcggaggcaggggcaag
BBa_J63007_sequence
1
ttggctttgaaattggctggtttggatatt
BBa_K1362059_sequence
1
gtctccgatgttggctttgaaattggctggtttggatattggcggaggcaggggcaagtgctagagacc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z