LOCUS       BBa_K1362091            2206 bp    DNA     linear       22-Nov-2024
DEFINITION  pSB1A30: High copy BioBrick cloning/expression backbone carrying 
            Amp resitance
ACCESSION   BBa_K1362091
VERSION     BBa_K1362091.1  
FEATURES             Location/Qualifiers
     misc_feature    1..21
                     /label=suffix
     misc_feature    22..93
                     /label=E. coli his operon terminator
     primer_bind     157..176
                     /label=VR
     stem_loop       30..64
                     /label=terminator stem loop
     misc_feature    261..875
                     /label=rep(pMB1) (rev)
     misc_feature    276..276
                     /label=ORI
     CDS             1035..1895
                     /label=ampR (rev)
     primer_bind     2018..2037
                     /label=VF2
     misc_feature    2094..2097
                     /label=end of terminator
     stem_loop       2107..2126
                     /label=terminator stem loop
     promoter        2139..2157
                     /label=T7 promotor
     misc_feature    2157..2184
                     /label=lacO reg
     misc_feature    2185..2206
                     /label=prefix
ORIGIN
        1 tactagtagc ggccgctgca gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt
       61 ttctttaaaa ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca ctgactcgct
      121 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
      181 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
      241 caggaaccgt aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga
      301 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
      361 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
      421 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
      481 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
      541 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
      601 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
      661 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
      721 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
      781 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
      841 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
      901 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
      961 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     1021 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     1081 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     1141 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     1201 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     1261 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     1321 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     1381 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     1441 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     1501 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     1561 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     1621 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     1681 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     1741 gctgttgaga tccagttcga tataacccac tcgtgcaccc aactgatctt cagcatcttt
     1801 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     1861 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     1921 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     1981 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
     2041 tattatcatg acattaacct ataaaaatag gcgtatcacg aggcagaatt tcagataaaa
     2101 aaaatcctta gctttcgcta aggatgattt ctggaattta atacgactca ctatagggga
     2161 attgtgagcg gataacaatt ccccgaattc gcggccgctt ctagag
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