##gff-version 3
##sequence-region BBa_K1362091 1 2206
BBa_K1362091	.	stem_loop	2107	2126	.	+	0	ID=annotation2404610;Name=terminator stem loop
BBa_K1362091	.	primer_binding_site	2018	2037	.	+	0	ID=annotation2404608;Name=VF2
BBa_K1362091	.	sequence_feature	2094	2097	.	+	0	ID=annotation2404609;Name=end of terminator
BBa_K1362091	.	sequence_feature	22	93	.	+	0	ID=annotation2404601;Name=E. coli his operon terminator
BBa_K1362091	.	operator	2157	2184	.	+	0	ID=annotation2404631;Name=lacO reg
BBa_K1362091	.	CDS	1035	1895	.	+	0	ID=annotation2404607;Name=ampR (rev)
BBa_K1362091	.	sequence_feature	276	276	.	+	0	ID=annotation2404606;Name=ORI
BBa_K1362091	.	stem_loop	30	64	.	+	0	ID=annotation2404603;Name=terminator stem loop
BBa_K1362091	.	engineered_region	2185	2206	.	+	0	ID=annotation2404632;Name=prefix
BBa_K1362091	.	engineered_region	1	21	.	+	0	ID=annotation2404600;Name=suffix
BBa_K1362091	.	primer_binding_site	157	176	.	+	0	ID=annotation2404602;Name=VR
BBa_K1362091	.	sequence_feature	261	875	.	+	0	ID=annotation2404605;Name=rep(pMB1) (rev)
BBa_K1362091	.	promoter	2139	2157	.	+	0	ID=annotation2404611;Name=T7 promotor
>BBa_K1362091
tactagtagcggccgctgcagtccggcaaaaaagggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaaga
ttacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctc
actcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaa
ggccaggaaccgtaaaaaggccgcgttgctggcgtttttccacaggctccgcccccctgacgagcatcacaaaaatcga
cgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgct
ctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctc
acgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgac
cgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactg
gtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactag
aagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaa
caaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatc
ctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaa
aaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtct
gacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactcc
ccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctc
accggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcc
tccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgcca
ttgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagt
tacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgca
gtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactg
gtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataa
taccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatctta
ccgctgttgagatccagttcgatataacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgttt
ctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatact
cttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaa
aataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacat
taacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctg
gaatttaatacgactcactataggggaattgtgagcggataacaattccccgaattcgcggccgcttctagag