pSB4A50
type: ComponentDefinition
persistent identity: BBa K1362095
display id: BBa_K1362095
version: 1
was generated by: iGEM to SBOL conversion
description: pSB4A50: Low copy BioBrick cloning/expression backbone carrying Amp resistance
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:04Z
owned by: james
owned by: myers
discontinued: false
dominant: false
experience: experience/Works
group u list: _1738_
m user id: 0
owner id: 22830
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
component: BBa_B0054
component: BBa_B0042
component: BBa_B0062
component: BBa_G00102
component: BBa_B0044
component: BBa_G00001
component: BBa_B0053
component: BBa_G0004
component: BBa_B0055
component: BBa_G00100
component: BBa_I50042
component: BBa_G0003
component: BBa_P1002
component: BBa_G0002
component: BBa_B0043
component: BBa_G00000
component: BBa_K1362463
sequence annotation: BBa_G00001
sequence annotation: BBa_B0044
sequence annotation: BBa_B0042
sequence annotation: BBa_B0054
sequence annotation: BBa_G00102
sequence annotation: BBa_B0062
sequence annotation: BBa_G0003
sequence annotation: BBa_I50042
sequence annotation: BBa_G0002
sequence annotation: BBa_P1002
sequence annotation: BBa_G0004
sequence annotation: BBa_B0053
sequence annotation: BBa_G00100
sequence annotation: BBa_B0055
sequence annotation: BBa_B0043
sequence annotation: BBa_K1362463
sequence annotation: BBa_G00000
sequence: https://synbiohub.org/public/igem/BBa_K1362095_sequence/1

BBa_B0054
type: Component
persistent identity: component2409779
display id: component2409779
version: 1
top level: pSB4A50
definition: BBa_B0054
access: public

BBa_B0042
type: Component
persistent identity: component2409776
display id: component2409776
version: 1
top level: pSB4A50
definition: TSS
access: public

BBa_B0062
type: Component
persistent identity: component2409783
display id: component2409783
version: 1
top level: pSB4A50
definition: BBa_B0062
access: public

BBa_G00102
type: Component
persistent identity: component2409781
display id: component2409781
version: 1
top level: pSB4A50
definition: VR
access: public

BBa_B0044
type: Component
persistent identity: component2409769
display id: component2409769
version: 1
top level: pSB4A50
definition: TOPO
access: public

BBa_G00001
type: Component
persistent identity: component2409764
display id: component2409764
version: 1
top level: pSB4A50
definition: Suffix
access: public

BBa_B0053
type: Component
persistent identity: component2409821
display id: component2409821
version: 1
top level: pSB4A50
definition: BBa_B0053
access: public

BBa_G0004
type: Component
persistent identity: component2409819
display id: component2409819
version: 1
top level: pSB4A50
definition: scar
access: public

BBa_B0055
type: Component
persistent identity: component2409826
display id: component2409826
version: 1
top level: pSB4A50
definition: BBa_B0055
access: public

BBa_G00100
type: Component
persistent identity: component2409823
display id: component2409823
version: 1
top level: pSB4A50
definition: VF2
access: public

BBa_I50042
type: Component
persistent identity: component2409812
display id: component2409812
version: 1
top level: pSB4A50
definition: pSC101
access: public

BBa_G0003
type: Component
persistent identity: component2409784
display id: component2409784
version: 1
top level: pSB4A50
definition: scar
access: public

BBa_P1002
type: Component
persistent identity: component2409818
display id: component2409818
version: 1
top level: pSB4A50
definition: ampR
access: public

BBa_G0002
type: Component
persistent identity: component2409813
display id: component2409813
version: 1
top level: pSB4A50
definition: SX scar
access: public

BBa_B0043
type: Component
persistent identity: component2409838
display id: component2409838
version: 1
top level: pSB4A50
definition: TOPO
access: public

BBa_B0042
type: Component
persistent identity: component2409833
display id: component2409833
version: 1
top level: pSB4A50
definition: TSS
access: public

BBa_G00000
type: Component
persistent identity: component2409847
display id: component2409847
version: 1
top level: pSB4A50
definition: Prefix
access: public

BBa_K1362463
type: Component
persistent identity: component2409842
display id: component2409842
version: 1
top level: pSB4A50
definition: T7/lac pro
access: public

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation2409764
display id: annotation2409764
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409764/range2409764/1
component: BBa_G00001

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409764/range2409764/1
type: Range
persistent identity: range2409764
display id: range2409764
version: 1
top level: pSB4A50
direction: direction/reverse
start: 1
end: 21
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation2409769
display id: annotation2409769
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409769/range2409769/1
component: BBa_B0044

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409769/range2409769/1
type: Range
persistent identity: range2409769
display id: range2409769
version: 1
top level: pSB4A50
direction: direction/reverse
start: 22
end: 34
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2409776
display id: annotation2409776
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409776/range2409776/1
component: BBa_B0042

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409776/range2409776/1
type: Range
persistent identity: range2409776
display id: range2409776
version: 1
top level: pSB4A50
direction: direction/reverse
start: 35
end: 46
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation2409779
display id: annotation2409779
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409779/range2409779/1
component: BBa_B0054

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409779/range2409779/1
type: Range
persistent identity: range2409779
display id: range2409779
version: 1
top level: pSB4A50
direction: direction/reverse
start: 47
end: 115
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation2409781
display id: annotation2409781
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409781/range2409781/1
component: BBa_G00102

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409781/range2409781/1
type: Range
persistent identity: range2409781
display id: range2409781
version: 1
top level: pSB4A50
direction: direction/reverse
start: 116
end: 135
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation2409783
display id: annotation2409783
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409783/range2409783/1
component: BBa_B0062

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409783/range2409783/1
type: Range
persistent identity: range2409783
display id: range2409783
version: 1
top level: pSB4A50
direction: direction/reverse
start: 136
end: 176
orientation: inline

BBa_G0003
type: SequenceAnnotation
persistent identity: annotation2409784
display id: annotation2409784
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409784/range2409784/1
component: BBa_G0003

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409784/range2409784/1
type: Range
persistent identity: range2409784
display id: range2409784
version: 1
top level: pSB4A50
direction: direction/reverse
start: 177
end: 183
orientation: inline

BBa_I50042
type: SequenceAnnotation
persistent identity: annotation2409812
display id: annotation2409812
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409812/range2409812/1
component: BBa_I50042

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409812/range2409812/1
type: Range
persistent identity: range2409812
display id: range2409812
version: 1
top level: pSB4A50
direction: direction/reverse
start: 184
end: 2220
orientation: inline

BBa_G0002
type: SequenceAnnotation
persistent identity: annotation2409813
display id: annotation2409813
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409813/range2409813/1
component: BBa_G0002

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409813/range2409813/1
type: Range
persistent identity: range2409813
display id: range2409813
version: 1
top level: pSB4A50
direction: direction/reverse
start: 2221
end: 2228
orientation: inline

BBa_P1002
type: SequenceAnnotation
persistent identity: annotation2409818
display id: annotation2409818
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409818/range2409818/1
component: BBa_P1002

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409818/range2409818/1
type: Range
persistent identity: range2409818
display id: range2409818
version: 1
top level: pSB4A50
direction: direction/reverse
start: 2229
end: 3171
orientation: inline

BBa_G0004
type: SequenceAnnotation
persistent identity: annotation2409819
display id: annotation2409819
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409819/range2409819/1
component: BBa_G0004

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409819/range2409819/1
type: Range
persistent identity: range2409819
display id: range2409819
version: 1
top level: pSB4A50
direction: direction/reverse
start: 3172
end: 3178
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation2409821
display id: annotation2409821
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409821/range2409821/1
component: BBa_B0053

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409821/range2409821/1
type: Range
persistent identity: range2409821
display id: range2409821
version: 1
top level: pSB4A50
direction: direction/reverse
start: 3179
end: 3250
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation2409823
display id: annotation2409823
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409823/range2409823/1
component: BBa_G00100

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409823/range2409823/1
type: Range
persistent identity: range2409823
display id: range2409823
version: 1
top level: pSB4A50
direction: direction/reverse
start: 3251
end: 3270
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation2409826
display id: annotation2409826
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409826/range2409826/1
component: BBa_B0055

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409826/range2409826/1
type: Range
persistent identity: range2409826
display id: range2409826
version: 1
top level: pSB4A50
direction: direction/reverse
start: 3271
end: 3348
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation2409833
display id: annotation2409833
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409833/range2409833/1
component: BBa_B0042

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409833/range2409833/1
type: Range
persistent identity: range2409833
display id: range2409833
version: 1
top level: pSB4A50
direction: direction/reverse
start: 3349
end: 3360
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation2409838
display id: annotation2409838
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409838/range2409838/1
component: BBa_B0043

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409838/range2409838/1
type: Range
persistent identity: range2409838
display id: range2409838
version: 1
top level: pSB4A50
direction: direction/reverse
start: 3361
end: 3373
orientation: inline

BBa_K1362463
type: SequenceAnnotation
persistent identity: annotation2409842
display id: annotation2409842
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409842/range2409842/1
component: BBa_K1362463

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409842/range2409842/1
type: Range
persistent identity: range2409842
display id: range2409842
version: 1
top level: pSB4A50
direction: direction/reverse
start: 3374
end: 3424
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation2409847
display id: annotation2409847
version: 1
top level: pSB4A50
location: https://synbiohub.org/public/igem/BBa_K1362095/annotation2409847/range2409847/1
component: BBa_G00000

https://synbiohub.org/public/igem/BBa_K1362095/annotation2409847/range2409847/1
type: Range
persistent identity: range2409847
display id: range2409847
version: 1
top level: pSB4A50
direction: direction/reverse
start: 3425
end: 3446
orientation: inline

TSS
type: ComponentDefinition
persistent identity: BBa B0042
display id: BBa_B0042
version: 1
was generated by: iGEM to SBOL conversion
description: Translational stop sequence
created: 2006-02-04T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: This part contains a stop codon in all 6 reading frames. Useful for ensuring that translation does not proceed through this part.
discontinued: false
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: stop codon (-1 frame)
sequence annotation: stop codon (+2 frame)
sequence annotation: stop codon (-3 frame)
sequence annotation: stop codon (+3 frame)
sequence annotation: stop codon (-2 frame)
sequence annotation: stop codon (+1 frame)
sequence: https://synbiohub.org/public/igem/BBa_B0042_sequence/1

stop codon (-1 frame)
type: SequenceAnnotation
persistent identity: annotation1797084
display id: annotation1797084
version: 1
top level: TSS
location: https://synbiohub.org/public/igem/BBa_B0042/annotation1797084/range1797084/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0042/annotation1797084/range1797084/1
type: Range
persistent identity: range1797084
display id: range1797084
version: 1
top level: TSS
direction: direction/reverse
start: 1
end: 3
orientation: inline

stop codon (+2 frame)
type: SequenceAnnotation
persistent identity: annotation1797083
display id: annotation1797083
version: 1
top level: TSS
location: https://synbiohub.org/public/igem/BBa_B0042/annotation1797083/range1797083/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0042/annotation1797083/range1797083/1
type: Range
persistent identity: range1797083
display id: range1797083
version: 1
top level: TSS
direction: direction/forward
start: 2
end: 4
orientation: inline

stop codon (-3 frame)
type: SequenceAnnotation
persistent identity: annotation1797088
display id: annotation1797088
version: 1
top level: TSS
location: https://synbiohub.org/public/igem/BBa_B0042/annotation1797088/range1797088/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0042/annotation1797088/range1797088/1
type: Range
persistent identity: range1797088
display id: range1797088
version: 1
top level: TSS
direction: direction/reverse
start: 5
end: 7
orientation: inline

stop codon (+3 frame)
type: SequenceAnnotation
persistent identity: annotation1797085
display id: annotation1797085
version: 1
top level: TSS
location: https://synbiohub.org/public/igem/BBa_B0042/annotation1797085/range1797085/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0042/annotation1797085/range1797085/1
type: Range
persistent identity: range1797085
display id: range1797085
version: 1
top level: TSS
direction: direction/forward
start: 6
end: 8
orientation: inline

stop codon (-2 frame)
type: SequenceAnnotation
persistent identity: annotation1797087
display id: annotation1797087
version: 1
top level: TSS
location: https://synbiohub.org/public/igem/BBa_B0042/annotation1797087/range1797087/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0042/annotation1797087/range1797087/1
type: Range
persistent identity: range1797087
display id: range1797087
version: 1
top level: TSS
direction: direction/reverse
start: 9
end: 11
orientation: inline

stop codon (+1 frame)
type: SequenceAnnotation
persistent identity: annotation1797086
display id: annotation1797086
version: 1
top level: TSS
location: https://synbiohub.org/public/igem/BBa_B0042/annotation1797086/range1797086/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0042/annotation1797086/range1797086/1
type: Range
persistent identity: range1797086
display id: range1797086
version: 1
top level: TSS
direction: direction/forward
start: 10
end: 12
orientation: inline

TOPO
type: ComponentDefinition
persistent identity: BBa B0043
display id: BBa_B0043
version: 1
was generated by: iGEM to SBOL conversion
description: Topoisomerase I mediated cloning site (forward)
created: 2006-02-04T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Insertion of this site will enable generation of a TOPO vector. The vector will have a T overhang and permit binding of topoisomerase I for direct cloning of PCR products. See http://openwetware.org/wiki/Topoisomerase_I_mediated_TA_cloning for a complete description of Topoisomerase I mediated cloning.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Topoisomerase I recognition site
sequence annotation: Generated T overhang
sequence annotation: Arbitrary sequence (could be any bases)
sequence annotation: Nt.BstNB I recognition site
sequence: https://synbiohub.org/public/igem/BBa_B0043_sequence/1

Topoisomerase I recognition site
type: SequenceAnnotation
persistent identity: annotation1797101
display id: annotation1797101
version: 1
top level: TOPO
location: https://synbiohub.org/public/igem/BBa_B0043/annotation1797101/range1797101/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0043/annotation1797101/range1797101/1
type: Range
persistent identity: range1797101
display id: range1797101
version: 1
top level: TOPO
direction: direction/forward
start: 1
end: 5
orientation: inline

Generated T overhang
type: SequenceAnnotation
persistent identity: annotation1797104
display id: annotation1797104
version: 1
top level: TOPO
location: https://synbiohub.org/public/igem/BBa_B0043/annotation1797104/range1797104/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0043/annotation1797104/range1797104/1
type: Range
persistent identity: range1797104
display id: range1797104
version: 1
top level: TOPO
direction: direction/forward
start: 5
end: 5
orientation: inline

Arbitrary sequence (could be any bases)
type: SequenceAnnotation
persistent identity: annotation1797103
display id: annotation1797103
version: 1
top level: TOPO
location: https://synbiohub.org/public/igem/BBa_B0043/annotation1797103/range1797103/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0043/annotation1797103/range1797103/1
type: Range
persistent identity: range1797103
display id: range1797103
version: 1
top level: TOPO
direction: direction/forward
start: 6
end: 8
orientation: inline

Nt.BstNB I recognition site
type: SequenceAnnotation
persistent identity: annotation1797102
display id: annotation1797102
version: 1
top level: TOPO
location: https://synbiohub.org/public/igem/BBa_B0043/annotation1797102/range1797102/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0043/annotation1797102/range1797102/1
type: Range
persistent identity: range1797102
display id: range1797102
version: 1
top level: TOPO
direction: direction/reverse
start: 9
end: 13
orientation: inline

TOPO
type: ComponentDefinition
persistent identity: BBa B0044
display id: BBa_B0044
version: 1
was generated by: iGEM to SBOL conversion
description: Topoisomerase I mediated cloning site (reverse)
created: 2006-02-04T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Insertion of this site will enable generation of a TOPO vector. The vector will have a T overhang and permit binding of topoisomerase I for direct cloning of PCR products. See http://openwetware.org/wiki/Topoisomerase_I_mediated_TA_cloning for a complete description of Topoisomerase I mediated cloning. This site belongs 3' of the cloning site. It is the reverse complement of BBa_B0043.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: Nt.BstNB I recognition site
sequence annotation: Arbitrary sequence (could be any bases)
sequence annotation: Topoisomerase I recognition site
sequence annotation: Generated T overhang
sequence: https://synbiohub.org/public/igem/BBa_B0044_sequence/1

Nt.BstNB I recognition site
type: SequenceAnnotation
persistent identity: annotation1797126
display id: annotation1797126
version: 1
top level: TOPO
location: https://synbiohub.org/public/igem/BBa_B0044/annotation1797126/range1797126/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0044/annotation1797126/range1797126/1
type: Range
persistent identity: range1797126
display id: range1797126
version: 1
top level: TOPO
direction: direction/forward
start: 1
end: 5
orientation: inline

Arbitrary sequence (could be any bases)
type: SequenceAnnotation
persistent identity: annotation1797127
display id: annotation1797127
version: 1
top level: TOPO
location: https://synbiohub.org/public/igem/BBa_B0044/annotation1797127/range1797127/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0044/annotation1797127/range1797127/1
type: Range
persistent identity: range1797127
display id: range1797127
version: 1
top level: TOPO
direction: direction/forward
start: 6
end: 8
orientation: inline

Topoisomerase I recognition site
type: SequenceAnnotation
persistent identity: annotation1797128
display id: annotation1797128
version: 1
top level: TOPO
location: https://synbiohub.org/public/igem/BBa_B0044/annotation1797128/range1797128/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_B0044/annotation1797128/range1797128/1
type: Range
persistent identity: range1797128
display id: range1797128
version: 1
top level: TOPO
direction: direction/reverse
start: 9
end: 13
orientation: inline

Generated T overhang
type: SequenceAnnotation
persistent identity: annotation1797129
display id: annotation1797129
version: 1
top level: TOPO
location: https://synbiohub.org/public/igem/BBa_B0044/annotation1797129/range1797129/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_B0044/annotation1797129/range1797129/1
type: Range
persistent identity: range1797129
display id: range1797129
version: 1
top level: TOPO
direction: direction/reverse
start: 9
end: 9
orientation: inline

BBa_B0053
type: ComponentDefinition
persistent identity: BBa B0053
display id: BBa_B0053
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (His)
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Terminator from the E.coli His operon
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Caitlin Conboy
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0053_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation318602
display id: annotation318602
version: 1
top level: BBa_B0053
location: https://synbiohub.org/public/igem/BBa_B0053/annotation318602/range318602/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0053/annotation318602/range318602/1
type: Range
persistent identity: range318602
display id: range318602
version: 1
top level: BBa_B0053
direction: direction/forward
start: 9
end: 43
orientation: inline

BBa_B0054
type: ComponentDefinition
persistent identity: BBa B0054
display id: BBa_B0054
version: 1
was generated by: iGEM to SBOL conversion
description: Transcriptional terminator
created: 2005-11-16T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Terminator to be placed downstream of multiple cloning site in new pSB series plasmids. Similar to BBa_B0051.
discontinued: false
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 44
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: identical to E. coli MG1655 between tonB and yciA
sequence annotation: stem loop
sequence: https://synbiohub.org/public/igem/BBa_B0054_sequence/1

identical to E. coli MG1655 between tonB and yciA
type: SequenceAnnotation
persistent identity: annotation1783342
display id: annotation1783342
version: 1
top level: BBa_B0054
location: https://synbiohub.org/public/igem/BBa_B0054/annotation1783342/range1783342/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0054/annotation1783342/range1783342/1
type: Range
persistent identity: range1783342
display id: range1783342
version: 1
top level: BBa_B0054
direction: direction/forward
start: 4
end: 43
orientation: inline

stem loop
type: SequenceAnnotation
persistent identity: annotation1747359
display id: annotation1747359
version: 1
top level: BBa_B0054
location: https://synbiohub.org/public/igem/BBa_B0054/annotation1747359/range1747359/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0054/annotation1747359/range1747359/1
type: Range
persistent identity: range1747359
display id: range1747359
version: 1
top level: BBa_B0054
direction: direction/forward
start: 11
end: 42
orientation: inline

BBa_B0055
type: ComponentDefinition
persistent identity: BBa B0055
display id: BBa_B0055
version: 1
was generated by: iGEM to SBOL conversion
description: -- No description --
created: 2005-11-16T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Terminator to be placed upstream of multiple cloning site in new pSB series plasmids.
discontinued: false
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 44
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: identical to bacteriophage T3
sequence annotation: stem loop from bacteriophage T3
sequence: https://synbiohub.org/public/igem/BBa_B0055_sequence/1

identical to bacteriophage T3
type: SequenceAnnotation
persistent identity: annotation1783311
display id: annotation1783311
version: 1
top level: BBa_B0055
location: https://synbiohub.org/public/igem/BBa_B0055/annotation1783311/range1783311/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0055/annotation1783311/range1783311/1
type: Range
persistent identity: range1783311
display id: range1783311
version: 1
top level: BBa_B0055
direction: direction/reverse
start: 28
end: 66
orientation: inline

stem loop from bacteriophage T3
type: SequenceAnnotation
persistent identity: annotation1783301
display id: annotation1783301
version: 1
top level: BBa_B0055
location: https://synbiohub.org/public/igem/BBa_B0055/annotation1783301/range1783301/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0055/annotation1783301/range1783301/1
type: Range
persistent identity: range1783301
display id: range1783301
version: 1
top level: BBa_B0055
direction: direction/reverse
start: 36
end: 57
orientation: inline

BBa_B0062
type: ComponentDefinition
persistent identity: BBa B0062
display id: BBa_B0062
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (Reverse B0052)
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:07:21Z
owned by: james
owned by: myers
mutable description: Reverse of B0052, E.coli transcriptional terminator from the ribosomal RNA rrnC operon.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Caitlin Conboy
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0062_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation318637
display id: annotation318637
version: 1
top level: BBa_B0062
location: https://synbiohub.org/public/igem/BBa_B0062/annotation318637/range318637/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0062/annotation318637/range318637/1
type: Range
persistent identity: range318637
display id: range318637
version: 1
top level: BBa_B0062
direction: direction/forward
start: 14
end: 33
orientation: inline

Prefix
type: ComponentDefinition
persistent identity: BBa G00000
display id: BBa_G00000
version: 1
was generated by: iGEM to SBOL conversion
description: BioBrick cloning site prefix
created: 2006-03-13T12:00:00Z
modified: 2015-08-31T04:07:27Z
owned by: james
owned by: myers
mutable description: This part is the standard BioBricks prefix.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: Prefix
sequence annotation: EcoRI site
sequence annotation: NotI site
sequence annotation: XbaI site
sequence: https://synbiohub.org/public/igem/BBa_G00000_sequence/1

Prefix
type: SequenceAnnotation
persistent identity: annotation1934225
display id: annotation1934225
version: 1
top level: Prefix
location: https://synbiohub.org/public/igem/BBa_G00000/annotation1934225/range1934225/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_G00000/annotation1934225/range1934225/1
type: Range
persistent identity: range1934225
display id: range1934225
version: 1
top level: Prefix
direction: direction/forward
start: 1
end: 22
orientation: inline

EcoRI site
type: SequenceAnnotation
persistent identity: annotation1934223
display id: annotation1934223
version: 1
top level: Prefix
location: https://synbiohub.org/public/igem/BBa_G00000/annotation1934223/range1934223/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_G00000/annotation1934223/range1934223/1
type: Range
persistent identity: range1934223
display id: range1934223
version: 1
top level: Prefix
direction: direction/forward
start: 1
end: 6
orientation: inline

NotI site
type: SequenceAnnotation
persistent identity: annotation1961228
display id: annotation1961228
version: 1
top level: Prefix
location: https://synbiohub.org/public/igem/BBa_G00000/annotation1961228/range1961228/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_G00000/annotation1961228/range1961228/1
type: Range
persistent identity: range1961228
display id: range1961228
version: 1
top level: Prefix
direction: direction/forward
start: 7
end: 14
orientation: inline

XbaI site
type: SequenceAnnotation
persistent identity: annotation1934224
display id: annotation1934224
version: 1
top level: Prefix
location: https://synbiohub.org/public/igem/BBa_G00000/annotation1934224/range1934224/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_G00000/annotation1934224/range1934224/1
type: Range
persistent identity: range1934224
display id: range1934224
version: 1
top level: Prefix
direction: direction/forward
start: 16
end: 21
orientation: inline

Suffix
type: ComponentDefinition
persistent identity: BBa G00001
display id: BBa_G00001
version: 1
was generated by: iGEM to SBOL conversion
description: BioBrick cloning site suffix
created: 2006-03-13T12:00:00Z
modified: 2015-08-31T04:07:27Z
owned by: james
owned by: myers
mutable description: This part is the standard BioBricks suffix.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Suffix
sequence annotation: SpeI
sequence annotation: NotI
sequence annotation: PstI
sequence: https://synbiohub.org/public/igem/BBa_G00001_sequence/1

Suffix
type: SequenceAnnotation
persistent identity: annotation1821544
display id: annotation1821544
version: 1
top level: Suffix
location: https://synbiohub.org/public/igem/BBa_G00001/annotation1821544/range1821544/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_G00001/annotation1821544/range1821544/1
type: Range
persistent identity: range1821544
display id: range1821544
version: 1
top level: Suffix
direction: direction/forward
start: 1
end: 21
orientation: inline

SpeI
type: SequenceAnnotation
persistent identity: annotation1934226
display id: annotation1934226
version: 1
top level: Suffix
location: https://synbiohub.org/public/igem/BBa_G00001/annotation1934226/range1934226/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_G00001/annotation1934226/range1934226/1
type: Range
persistent identity: range1934226
display id: range1934226
version: 1
top level: Suffix
direction: direction/forward
start: 2
end: 7
orientation: inline

NotI
type: SequenceAnnotation
persistent identity: annotation1934228
display id: annotation1934228
version: 1
top level: Suffix
location: https://synbiohub.org/public/igem/BBa_G00001/annotation1934228/range1934228/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_G00001/annotation1934228/range1934228/1
type: Range
persistent identity: range1934228
display id: range1934228
version: 1
top level: Suffix
direction: direction/forward
start: 9
end: 16
orientation: inline

PstI
type: SequenceAnnotation
persistent identity: annotation1934227
display id: annotation1934227
version: 1
top level: Suffix
location: https://synbiohub.org/public/igem/BBa_G00001/annotation1934227/range1934227/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_G00001/annotation1934227/range1934227/1
type: Range
persistent identity: range1934227
display id: range1934227
version: 1
top level: Suffix
direction: direction/forward
start: 16
end: 21
orientation: inline

VF2
type: ComponentDefinition
persistent identity: BBa G00100
display id: BBa_G00100
version: 1
was generated by: iGEM to SBOL conversion
description: Forward primer for sequencing/amplifying BioBrick parts (VF2)
created: 2004-05-24T11:00:00Z
modified: 2015-08-31T04:07:28Z
owned by: james
owned by: myers
mutable description: Originally named VF2, primer binds upstream of the standard BBa_MCS on biobrick vectors.
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Tom Knight
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence annotation: VF2
sequence: https://synbiohub.org/public/igem/BBa_G00100_sequence/1

VF2
type: SequenceAnnotation
persistent identity: annotation1934505
display id: annotation1934505
version: 1
top level: VF2
location: https://synbiohub.org/public/igem/BBa_G00100/annotation1934505/range1934505/1
role: SO:0005850
role: feature/primer binding

https://synbiohub.org/public/igem/BBa_G00100/annotation1934505/range1934505/1
type: Range
persistent identity: range1934505
display id: range1934505
version: 1
top level: VF2
direction: direction/forward
start: 1
end: 20
orientation: inline

VR
type: ComponentDefinition
persistent identity: BBa G00102
display id: BBa_G00102
version: 1
was generated by: iGEM to SBOL conversion
description: Reverse BioBrick primer annealing site (VR binding site)
created: 2006-02-03T12:00:00Z
modified: 2015-08-31T04:07:28Z
owned by: james
owned by: myers
mutable description: This is the VR sequence to be used in construction of plasmids which include the VR primer site. It is the reverse complement of BBa_G00101.
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: VR
sequence: https://synbiohub.org/public/igem/BBa_G00102_sequence/1

VR
type: SequenceAnnotation
persistent identity: annotation1961229
display id: annotation1961229
version: 1
top level: VR
location: https://synbiohub.org/public/igem/BBa_G00102/annotation1961229/range1961229/1
role: feature/primer binding
role: SO:0005850

https://synbiohub.org/public/igem/BBa_G00102/annotation1961229/range1961229/1
type: Range
persistent identity: range1961229
display id: range1961229
version: 1
top level: VR
direction: direction/reverse
start: 1
end: 20
orientation: inline

ampR
type: ComponentDefinition
persistent identity: BBa P1002
display id: BBa_P1002
version: 1
was generated by: iGEM to SBOL conversion
description: ampicillin resistance cassette
created: 2006-01-23T12:00:00Z
modified: 2015-05-08T01:14:11Z
owned by: james
owned by: myers
mutable description: Ampicillin resistance cassette for modular BioBricks vector construction.
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: It's complicated
status: status/Planning
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Generator
role: SO:0000804
sequence annotation: ampR -35
sequence annotation: ampR -10
sequence annotation: ampR RBS
sequence annotation: ampicillin resistance
sequence: https://synbiohub.org/public/igem/BBa_P1002_sequence/1

ampR -35
type: SequenceAnnotation
persistent identity: annotation1897109
display id: annotation1897109
version: 1
top level: ampR
location: https://synbiohub.org/public/igem/BBa_P1002/annotation1897109/range1897109/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_P1002/annotation1897109/range1897109/1
type: Range
persistent identity: range1897109
display id: range1897109
version: 1
top level: ampR
direction: direction/forward
start: 31
end: 36
orientation: inline

ampR -10
type: SequenceAnnotation
persistent identity: annotation1897110
display id: annotation1897110
version: 1
top level: ampR
location: https://synbiohub.org/public/igem/BBa_P1002/annotation1897110/range1897110/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_P1002/annotation1897110/range1897110/1
type: Range
persistent identity: range1897110
display id: range1897110
version: 1
top level: ampR
direction: direction/forward
start: 57
end: 62
orientation: inline

ampR RBS
type: SequenceAnnotation
persistent identity: annotation1897111
display id: annotation1897111
version: 1
top level: ampR
location: https://synbiohub.org/public/igem/BBa_P1002/annotation1897111/range1897111/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_P1002/annotation1897111/range1897111/1
type: Range
persistent identity: range1897111
display id: range1897111
version: 1
top level: ampR
direction: direction/forward
start: 71
end: 75
orientation: inline

ampicillin resistance
type: SequenceAnnotation
persistent identity: annotation1790786
display id: annotation1790786
version: 1
top level: ampR
location: https://synbiohub.org/public/igem/BBa_P1002/annotation1790786/range1790786/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_P1002/annotation1790786/range1790786/1
type: Range
persistent identity: range1790786
display id: range1790786
version: 1
top level: ampR
direction: direction/forward
start: 80
end: 943
orientation: inline

pSC101
type: ComponentDefinition
persistent identity: BBa I50042
display id: BBa_I50042
version: 1
was generated by: iGEM to SBOL conversion
description: pSC101 origin of replication
created: 2007-04-10T11:00:00Z
modified: 2015-08-31T04:07:42Z
mutable provenance: This part was amplified from pSB4A3-P1010.
owned by: james
owned by: myers
mutable description: pSC101 origin of replication which is supposed to have low copy number. This was the origin used in Michael Elowitz's repressilator and in Bujard's pZS4Int-1 vector with mutations introduced to eliminate a SpeI site. It is also the origin of replication in pSB4* series plasmids.
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The replication origin boundaries were chosen quite liberally (i.e. they might include more sequence than is strictly necessary) because a different version of this origin (BBa_I50040) was shown to be non-functional.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: Terminator
sequence annotation: par region
sequence annotation: inverted repeat
sequence annotation: Tn3 integration site
sequence annotation: AT rich region
sequence annotation: direct repeat
sequence annotation: mutated SpeI site
sequence annotation: -35
sequence annotation: promoter region
sequence annotation: -10
sequence annotation: RBS
sequence annotation: repA
sequence annotation: G to A difference with canonical pSC101 vector sequence
sequence annotation: C to T difference with canonical pSC101 vector sequence
sequence annotation: G to C difference with canonical pSC101 vector sequence
sequence annotation: putative polyA
sequence annotation: T to C difference with canonical pSC101 vector sequence
sequence: https://synbiohub.org/public/igem/BBa_I50042_sequence/1

Terminator
type: SequenceAnnotation
persistent identity: annotation2071309
display id: annotation2071309
version: 1
top level: pSC101
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071309/range2071309/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_I50042/annotation2071309/range2071309/1
type: Range
persistent identity: range2071309
display id: range2071309
version: 1
top level: pSC101
direction: direction/forward
start: 46
end: 75
orientation: inline

par region
type: SequenceAnnotation
persistent identity: annotation2071310
display id: annotation2071310
version: 1
top level: pSC101
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071310/range2071310/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_I50042/annotation2071310/range2071310/1
type: Range
persistent identity: range2071310
display id: range2071310
version: 1
top level: pSC101
direction: direction/forward
start: 137
end: 504
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation2071311
display id: annotation2071311
version: 1
top level: pSC101
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071311/range2071311/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_I50042/annotation2071311/range2071311/1
type: Range
persistent identity: range2071311
display id: range2071311
version: 1
top level: pSC101
direction: direction/forward
start: 139
end: 152
orientation: inline

Tn3 integration site
type: SequenceAnnotation
persistent identity: annotation2071312
display id: annotation2071312
version: 1
top level: pSC101
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071312/range2071312/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_I50042/annotation2071312/range2071312/1
type: Range
persistent identity: range2071312
display id: range2071312
version: 1
top level: pSC101
direction: direction/forward
start: 158
end: 162
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation2071313
display id: annotation2071313
version: 1
top level: pSC101
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071313/range2071313/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_I50042/annotation2071313/range2071313/1
type: Range
persistent identity: range2071313
display id: range2071313
version: 1
top level: pSC101
direction: direction/forward
start: 165
end: 181
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation2071314
display id: annotation2071314
version: 1
top level: pSC101
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071314/range2071314/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_I50042/annotation2071314/range2071314/1
type: Range
persistent identity: range2071314
display id: range2071314
version: 1
top level: pSC101
direction: direction/forward
start: 192
end: 215
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation2071315
display id: annotation2071315
version: 1
top level: pSC101
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071315/range2071315/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_I50042/annotation2071315/range2071315/1
type: Range
persistent identity: range2071315
display id: range2071315
version: 1
top level: pSC101
direction: direction/forward
start: 221
end: 238
orientation: inline

AT rich region
type: SequenceAnnotation
persistent identity: annotation2071316
display id: annotation2071316
version: 1
top level: pSC101
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071316/range2071316/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_I50042/annotation2071316/range2071316/1
type: Range
persistent identity: range2071316
display id: range2071316
version: 1
top level: pSC101
direction: direction/forward
start: 635
end: 717
orientation: inline

direct repeat
type: SequenceAnnotation
persistent identity: annotation2071317
display id: annotation2071317
version: 1
top level: pSC101
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071317/range2071317/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_I50042/annotation2071317/range2071317/1
type: Range
persistent identity: range2071317
display id: range2071317
version: 1
top level: pSC101
direction: direction/forward
start: 716
end: 735
orientation: inline

direct repeat
type: SequenceAnnotation
persistent identity: annotation2071318
display id: annotation2071318
version: 1
top level: pSC101
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071318/range2071318/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I50042/annotation2071318/range2071318/1
type: Range
persistent identity: range2071318
display id: range2071318
version: 1
top level: pSC101
direction: direction/forward
start: 737
end: 756
orientation: inline

direct repeat
type: SequenceAnnotation
persistent identity: annotation2071319
display id: annotation2071319
version: 1
top level: pSC101
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071319/range2071319/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I50042/annotation2071319/range2071319/1
type: Range
persistent identity: range2071319
display id: range2071319
version: 1
top level: pSC101
direction: direction/forward
start: 769
end: 788
orientation: inline

mutated SpeI site
type: SequenceAnnotation
persistent identity: annotation2071320
display id: annotation2071320
version: 1
top level: pSC101
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071320/range2071320/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_I50042/annotation2071320/range2071320/1
type: Range
persistent identity: range2071320
display id: range2071320
version: 1
top level: pSC101
direction: direction/reverse
start: 814
end: 819
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2071321
display id: annotation2071321
version: 1
top level: pSC101
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071321/range2071321/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I50042/annotation2071321/range2071321/1
type: Range
persistent identity: range2071321
display id: range2071321
version: 1
top level: pSC101
direction: direction/forward
start: 828
end: 833
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation2071322
display id: annotation2071322
version: 1
top level: pSC101
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071322/range2071322/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I50042/annotation2071322/range2071322/1
type: Range
persistent identity: range2071322
display id: range2071322
version: 1
top level: pSC101
direction: direction/forward
start: 838
end: 859
orientation: inline

promoter region
type: SequenceAnnotation
persistent identity: annotation2071323
display id: annotation2071323
version: 1
top level: pSC101
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071323/range2071323/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_I50042/annotation2071323/range2071323/1
type: Range
persistent identity: range2071323
display id: range2071323
version: 1
top level: pSC101
direction: direction/forward
start: 846
end: 865
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2071324
display id: annotation2071324
version: 1
top level: pSC101
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071324/range2071324/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_I50042/annotation2071324/range2071324/1
type: Range
persistent identity: range2071324
display id: range2071324
version: 1
top level: pSC101
direction: direction/forward
start: 850
end: 855
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation2071325
display id: annotation2071325
version: 1
top level: pSC101
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071325/range2071325/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I50042/annotation2071325/range2071325/1
type: Range
persistent identity: range2071325
display id: range2071325
version: 1
top level: pSC101
direction: direction/forward
start: 862
end: 884
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2071326
display id: annotation2071326
version: 1
top level: pSC101
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071326/range2071326/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_I50042/annotation2071326/range2071326/1
type: Range
persistent identity: range2071326
display id: range2071326
version: 1
top level: pSC101
direction: direction/forward
start: 870
end: 882
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2071327
display id: annotation2071327
version: 1
top level: pSC101
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071327/range2071327/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_I50042/annotation2071327/range2071327/1
type: Range
persistent identity: range2071327
display id: range2071327
version: 1
top level: pSC101
direction: direction/forward
start: 879
end: 884
orientation: inline

repA
type: SequenceAnnotation
persistent identity: annotation2071328
display id: annotation2071328
version: 1
top level: pSC101
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071328/range2071328/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I50042/annotation2071328/range2071328/1
type: Range
persistent identity: range2071328
display id: range2071328
version: 1
top level: pSC101
direction: direction/forward
start: 886
end: 1836
orientation: inline

G to A difference with canonical pSC101 vector sequence
type: SequenceAnnotation
persistent identity: annotation2071329
display id: annotation2071329
version: 1
top level: pSC101
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071329/range2071329/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_I50042/annotation2071329/range2071329/1
type: Range
persistent identity: range2071329
display id: range2071329
version: 1
top level: pSC101
direction: direction/forward
start: 1655
end: 1655
orientation: inline

C to T difference with canonical pSC101 vector sequence
type: SequenceAnnotation
persistent identity: annotation2071330
display id: annotation2071330
version: 1
top level: pSC101
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071330/range2071330/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_I50042/annotation2071330/range2071330/1
type: Range
persistent identity: range2071330
display id: range2071330
version: 1
top level: pSC101
direction: direction/forward
start: 1750
end: 1750
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation2071331
display id: annotation2071331
version: 1
top level: pSC101
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071331/range2071331/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_I50042/annotation2071331/range2071331/1
type: Range
persistent identity: range2071331
display id: range2071331
version: 1
top level: pSC101
direction: direction/forward
start: 1847
end: 1861
orientation: inline

G to C difference with canonical pSC101 vector sequence
type: SequenceAnnotation
persistent identity: annotation2071332
display id: annotation2071332
version: 1
top level: pSC101
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071332/range2071332/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_I50042/annotation2071332/range2071332/1
type: Range
persistent identity: range2071332
display id: range2071332
version: 1
top level: pSC101
direction: direction/forward
start: 1912
end: 1912
orientation: inline

putative polyA
type: SequenceAnnotation
persistent identity: annotation2071333
display id: annotation2071333
version: 1
top level: pSC101
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071333/range2071333/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_I50042/annotation2071333/range2071333/1
type: Range
persistent identity: range2071333
display id: range2071333
version: 1
top level: pSC101
direction: direction/forward
start: 1947
end: 1962
orientation: inline

inverted repeat
type: SequenceAnnotation
persistent identity: annotation2071334
display id: annotation2071334
version: 1
top level: pSC101
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071334/range2071334/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_I50042/annotation2071334/range2071334/1
type: Range
persistent identity: range2071334
display id: range2071334
version: 1
top level: pSC101
direction: direction/forward
start: 1963
end: 1979
orientation: inline

T to C difference with canonical pSC101 vector sequence
type: SequenceAnnotation
persistent identity: annotation2071335
display id: annotation2071335
version: 1
top level: pSC101
location: https://synbiohub.org/public/igem/BBa_I50042/annotation2071335/range2071335/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_I50042/annotation2071335/range2071335/1
type: Range
persistent identity: range2071335
display id: range2071335
version: 1
top level: pSC101
direction: direction/forward
start: 2002
end: 2002
orientation: inline

SX scar
type: ComponentDefinition
persistent identity: BBa G0002
display id: BBa_G0002
version: 1
was generated by: iGEM to SBOL conversion
description: SpeI/XbaI mixed site
created: 2007-02-26T12:00:00Z
modified: 2015-08-31T04:07:27Z
mutable provenance: XbaI and SpeI sites
owned by: james
owned by: myers
mutable description: XbaI/SpeI mixed site. Simply used to aid in entry of parts into the registry.
discontinued: false
dominant: true
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_G0002_sequence/1

scar
type: ComponentDefinition
persistent identity: BBa G0003
display id: BBa_G0003
version: 1
was generated by: iGEM to SBOL conversion
description: NheI/XbaI mixed site
created: 2007-02-26T12:00:00Z
modified: 2015-08-31T04:07:27Z
mutable provenance: NheI and XbaI
owned by: james
owned by: myers
mutable description: NheI/XbaI mixed site
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_G0003_sequence/1

scar
type: ComponentDefinition
persistent identity: BBa G0004
display id: BBa_G0004
version: 1
was generated by: iGEM to SBOL conversion
description: SpeI/NheI mixed site
created: 2007-02-26T12:00:00Z
modified: 2015-08-31T04:07:27Z
mutable provenance: NheI and SpeI
owned by: james
owned by: myers
mutable description: NheI/SpeI mixed site. Used to help enter parts into the registry.
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_G0004_sequence/1

T7/lac pro
type: ComponentDefinition
persistent identity: BBa K1362463
display id: BBa_K1362463
version: 1
was generated by: iGEM to SBOL conversion
description: strong T7 promoter + LacO Regulator
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:06Z
owned by: james
owned by: myers
mutable description: GAATTTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCC
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 22830
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: scar of cloning into EcoRI site
sequence annotation: strong T7 promotor
sequence annotation: lacO reg
sequence: https://synbiohub.org/public/igem/BBa_K1362463_sequence/1

scar of cloning into EcoRI site
type: SequenceAnnotation
persistent identity: annotation2405286
display id: annotation2405286
version: 1
top level: T7/lac pro
location: https://synbiohub.org/public/igem/BBa_K1362463/annotation2405286/range2405286/1
role: feature/scar

https://synbiohub.org/public/igem/BBa_K1362463/annotation2405286/range2405286/1
type: Range
persistent identity: range2405286
display id: range2405286
version: 1
top level: T7/lac pro
direction: direction/forward
start: 1
end: 6
orientation: inline

strong T7 promotor
type: SequenceAnnotation
persistent identity: annotation2405261
display id: annotation2405261
version: 1
top level: T7/lac pro
location: https://synbiohub.org/public/igem/BBa_K1362463/annotation2405261/range2405261/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K1362463/annotation2405261/range2405261/1
type: Range
persistent identity: range2405261
display id: range2405261
version: 1
top level: T7/lac pro
direction: direction/forward
start: 6
end: 24
orientation: inline

lacO reg
type: SequenceAnnotation
persistent identity: annotation2405276
display id: annotation2405276
version: 1
top level: T7/lac pro
location: https://synbiohub.org/public/igem/BBa_K1362463/annotation2405276/range2405276/1
role: feature/operator
role: SO:0000057

https://synbiohub.org/public/igem/BBa_K1362463/annotation2405276/range2405276/1
type: Range
persistent identity: range2405276
display id: range2405276
version: 1
top level: T7/lac pro
direction: direction/forward
start: 24
end: 51
orientation: inline

https://synbiohub.org/public/igem/BBa_K1362095_sequence/1
type: Sequence
persistent identity: BBa K1362095 sequence
display id: BBa_K1362095_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcaggagtcactaagggttagttagttagattagcagaaagtcaaaagcctccgaccggaggcttttgactaaaacttcccttggggttatcattggggctcactcaaaggcggtaatcagataaaaaaaatccttagctttcgctaaggatgatttctgctagagctgtcagaccaagtttacgagctcgcttggactcctgttgatagatccagtaatgacctcagaactccatctggatttgttcagaacgctcggttgccgccgggcgttttttattggtgagaatccaagcactagggacagtaagacgggtaagcctgttgatgataccgctgccttactgggtgcattagccagtctgaatgacctgtcacgggataatccgaagtggtcagactggaaaatcagagggcaggaactgctgaacagcaaaaagtcagatagcaccacatagcagacccgccataaaacgccctgagaagcccgtgacgggcttttcttgtattatgggtagtttccttgcatgaatccataaaaggcgcctgtagtgccatttacccccattcactgccagagccgtgagcgcagcgaactgaatgtcacgaaaaagacagcgactcaggtgcctgatggtcggagacaaaaggaatattcagcgatttgcccgagcttgcgagggtgctacttaagcctttagggttttaaggtctgttttgtagaggagcaaacagcgtttgcgacatccttttgtaatactgcggaactgactaaagtagtgagttatacacagggctgggatctattctttttatctttttttattctttctttattctataaattataaccacttgaatataaacaaaaaaaacacacaaaggtctagcggaatttacagagggtctagcagaatttacaagttttccagcaaaggtctagcagaatttacagatacccacaactcaaaggaaaaggacatgtaattatcattgactagcccatctcaattggtatagtgattaaaatcacctagaccaattgagatgtatgtctgaattagttgttttcaaagcaaatgaactagcgattagtcgctatgacttaacggagcatgaaaccaagctaattttatgctgtgtggcactactcaaccccacgattgaaaaccctacaaggaaagaacggacggtatcgttcacttataaccaatacgctcagatgatgaacatcagtagggaaaatgcttatggtgtattagctaaagcaaccagagagctgatgacgagaactgtggaaatcaggaatcctttggttaaaggctttgagattttccagtggacaaactatgccaagttctcaagcgaaaaattagaattagtttttagtgaagagatattgccttatcttttccagttaaaaaaattcataaaatataatctggaacatgttaagtcttttgaaaacaaatactctatgaggatttatgagtggttattaaaagaactaacacaaaagaaaactcacaaggcaaatatagagattagccttgatgaatttaagttcatgttaatgcttgaaaataactaccatgagtttaaaaggcttaaccaatgggttttgaaaccaataagtaaagatttaaacacttacagcaatatgaaattggtggttgataagcgaggccgcccgactgatacgttgattttccaagttgaactagatagacaaatggatctcgtaaccgaacttgagaacaaccagataaaaatgaatggtgacaaaataccaacaaccattacatcagattcctacctacataacggactaagaaaaacactacacgatgctttaactgcaaaaattcagctcaccagttttgaggcaaaatttttgagtgacatgcaaagtaagtatgatctcaatggttcgttctcatggctcacgcaaaaacaacgaaccacactagagaacatactggc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0042_sequence/1
type: Sequence
persistent identity: BBa B0042 sequence
display id: BBa_B0042_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttagttagttag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0043_sequence/1
type: Sequence
persistent identity: BBa B0043 sequence
display id: BBa_B0043_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cccttagtgactc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0044_sequence/1
type: Sequence
persistent identity: BBa B0044 sequence
display id: BBa_B0044_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gagtcactaaggg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0053_sequence/1
type: Sequence
persistent identity: BBa B0053 sequence
display id: BBa_B0053_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0054_sequence/1
type: Sequence
persistent identity: BBa B0054 sequence
display id: BBa_B0054_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attagcagaaagtcaaaagcctccgaccggaggcttttgactaaaacttcccttggggttatcattggg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0055_sequence/1
type: Sequence
persistent identity: BBa B0055 sequence
display id: BBa_B0055_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0062_sequence/1
type: Sequence
persistent identity: BBa B0062 sequence
display id: BBa_B0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cagataaaaaaaatccttagctttcgctaaggatgatttct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00000_sequence/1
type: Sequence
persistent identity: BBa G00000 sequence
display id: BBa_G00000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaattcgcggccgcttctagag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00001_sequence/1
type: Sequence
persistent identity: BBa G00001 sequence
display id: BBa_G00001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00100_sequence/1
type: Sequence
persistent identity: BBa G00100 sequence
display id: BBa_G00100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgccacctgacgtctaagaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00102_sequence/1
type: Sequence
persistent identity: BBa G00102 sequence
display id: BBa_G00102_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctcactcaaaggcggtaat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_P1002_sequence/1
type: Sequence
persistent identity: BBa P1002 sequence
display id: BBa_P1002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctaaatacattcaaatatctatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagaatatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgccgaagatcagttgggtgcacgtgtgggttacatcgaactggacctcaacagcggtaagattcttgagagttttcgccccgaagaacgtttcccaatgatgagcacttttaaagttctgctctgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggacggcatgacagtacgcgaattatgcagcgctgccataaccatgagtgataacacggcggccaacttacttctgacaacgatcggaggaccgaaggagcttaccgcttttttgcacaacatgggtgatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagctatggcaacaacgttgcgcaaactcttaactggcgaacttcttactctcgcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtcccgcggtattattgcagccctggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagccaggcaactatggacgaacgtaatcgccagatcgctgagataggtgcctccctgattaagcattggtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I50042_sequence/1
type: Sequence
persistent identity: BBa I50042 sequence
display id: BBa_I50042_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctgtcagaccaagtttacgagctcgcttggactcctgttgatagatccagtaatgacctcagaactccatctggatttgttcagaacgctcggttgccgccgggcgttttttattggtgagaatccaagcactagggacagtaagacgggtaagcctgttgatgataccgctgccttactgggtgcattagccagtctgaatgacctgtcacgggataatccgaagtggtcagactggaaaatcagagggcaggaactgctgaacagcaaaaagtcagatagcaccacatagcagacccgccataaaacgccctgagaagcccgtgacgggcttttcttgtattatgggtagtttccttgcatgaatccataaaaggcgcctgtagtgccatttacccccattcactgccagagccgtgagcgcagcgaactgaatgtcacgaaaaagacagcgactcaggtgcctgatggtcggagacaaaaggaatattcagcgatttgcccgagcttgcgagggtgctacttaagcctttagggttttaaggtctgttttgtagaggagcaaacagcgtttgcgacatccttttgtaatactgcggaactgactaaagtagtgagttatacacagggctgggatctattctttttatctttttttattctttctttattctataaattataaccacttgaatataaacaaaaaaaacacacaaaggtctagcggaatttacagagggtctagcagaatttacaagttttccagcaaaggtctagcagaatttacagatacccacaactcaaaggaaaaggacatgtaattatcattgactagcccatctcaattggtatagtgattaaaatcacctagaccaattgagatgtatgtctgaattagttgttttcaaagcaaatgaactagcgattagtcgctatgacttaacggagcatgaaaccaagctaattttatgctgtgtggcactactcaaccccacgattgaaaaccctacaaggaaagaacggacggtatcgttcacttataaccaatacgctcagatgatgaacatcagtagggaaaatgcttatggtgtattagctaaagcaaccagagagctgatgacgagaactgtggaaatcaggaatcctttggttaaaggctttgagattttccagtggacaaactatgccaagttctcaagcgaaaaattagaattagtttttagtgaagagatattgccttatcttttccagttaaaaaaattcataaaatataatctggaacatgttaagtcttttgaaaacaaatactctatgaggatttatgagtggttattaaaagaactaacacaaaagaaaactcacaaggcaaatatagagattagccttgatgaatttaagttcatgttaatgcttgaaaataactaccatgagtttaaaaggcttaaccaatgggttttgaaaccaataagtaaagatttaaacacttacagcaatatgaaattggtggttgataagcgaggccgcccgactgatacgttgattttccaagttgaactagatagacaaatggatctcgtaaccgaacttgagaacaaccagataaaaatgaatggtgacaaaataccaacaaccattacatcagattcctacctacataacggactaagaaaaacactacacgatgctttaactgcaaaaattcagctcaccagttttgaggcaaaatttttgagtgacatgcaaagtaagtatgatctcaatggttcgttctcatggctcacgcaaaaacaacgaaccacactagagaacatactggctaaatacggaaggatctgaggttcttatggctcttgtatctatcagtgaagcatcaagactaacaaacaaaagtagaacaactgttcaccgttacatatcaaagggaaaactgtccatatgcacagatgaaaacggtgtaaaaaagatagatacatcagagcttttacgagtttttggtgcat…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G0002_sequence/1
type: Sequence
persistent identity: BBa G0002 sequence
display id: BBa_G0002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G0003_sequence/1
type: Sequence
persistent identity: BBa G0003 sequence
display id: BBa_G0003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctagag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G0004_sequence/1
type: Sequence
persistent identity: BBa G0004 sequence
display id: BBa_G0004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1362463_sequence/1
type: Sequence
persistent identity: BBa K1362463 sequence
display id: BBa_K1362463_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaatttaatacgactcactataggggaattgtgagcggataacaattcccc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z