LOCUS BBa_K1362096 3408 bp DNA linear 26-Apr-2024 DEFINITION pSB4C50: Low(?) copy BioBrick cloning/expression backbone carrying Cm resistance ACCESSION BBa_K1362096 VERSION BBa_K1362096.1 FEATURES Location/Qualifiers misc_feature 1..21 /label=Suffix misc_feature 2..7 /label=SpeI misc_feature 16..21 /label=PstI misc_feature 9..16 /label=NotI misc_feature 1..21 /label=BBa_G00001 misc_feature 30..34 /label=Nt.BstNB I recognition site misc_feature 35..37 /label=Arbitrary sequence (could be any bases) misc_feature 38..42 /label=Topoisomerase I recognition site misc_feature 38..38 /label=Generated T overhang misc_feature 30..42 /label=BBa_B0044 misc_feature 52..54 /label=stop codon (+2 frame) misc_feature 51..53 /label=stop codon (-1 frame) misc_feature 56..58 /label=stop codon (+3 frame) misc_feature 60..62 /label=stop codon (+1 frame) misc_feature 59..61 /label=stop codon (-2 frame) misc_feature 55..57 /label=stop codon (-3 frame) misc_feature 51..62 /label=BBa_B0042 stem_loop 81..112 /label=stem loop misc_feature 74..113 /label=identical to E. coli MG1655 between tonB and yciA terminator 71..139 /label=BBa_B0054 primer_bind 148..167 /label=VR misc_feature 148..167 /label=BBa_G00102 stem_loop 189..208 /label=stem_loop terminator 176..216 /label=BBa_B0062 misc_feature 225..231 /label=BBa_G0003 stem_loop 285..314 /label=Terminator misc_feature 376..743 /label=par region misc_feature 378..391 /label=inverted repeat misc_feature 397..401 /label=Tn3 integration site misc_feature 404..420 /label=inverted repeat misc_feature 431..454 /label=inverted repeat misc_feature 460..477 /label=inverted repeat misc_feature 874..956 /label=AT rich region misc_feature 955..974 /label=direct repeat misc_feature 976..995 /label=direct repeat misc_feature 1008..1027 /label=direct repeat misc_feature 1053..1058 /label=mutated SpeI site promoter 1067..1072 /label=-35 misc_feature 1077..1098 /label=inverted repeat promoter 1085..1104 /label=promoter region promoter 1089..1094 /label=-10 misc_feature 1101..1123 /label=inverted repeat RBS 1109..1121 /label=RBS RBS 1118..1123 /label=RBS CDS 1125..2075 /label=repA misc_feature 1894..1894 /label=G to A difference with canonical pSC101 vector sequence misc_feature 1989..1989 /label=C to T difference with canonical pSC101 vector sequence misc_feature 2086..2100 /label=inverted repeat misc_feature 2151..2151 /label=G to C difference with canonical pSC101 vector sequence polyA_site 2186..2201 /label=putative polyA misc_feature 2202..2218 /label=inverted repeat misc_feature 2241..2241 /label=T to C difference with canonical pSC101 vector sequence misc_feature 240..2276 /label=BBa_I50042 misc_feature 2285..2292 /label=BBa_G0002 CDS 2407..3069 /label=cmR misc_feature 2301..3069 /label=BBa_P1004 misc_feature 3078..3084 /label=BBa_G0004 stem_loop 3101..3135 /label=stem_loop terminator 3093..3164 /label=BBa_B0053 primer_bind 3173..3192 /label=VF2 primer 3173..3192 /label=BBa_G00100 stem_loop 3236..3257 /label=stem loop from bacteriophage T3 misc_feature 3228..3266 /label=identical to bacteriophage T3 terminator 3201..3278 /label=BBa_B0055 misc_feature 3288..3290 /label=stop codon (+2 frame) misc_feature 3287..3289 /label=stop codon (-1 frame) misc_feature 3292..3294 /label=stop codon (+3 frame) misc_feature 3296..3298 /label=stop codon (+1 frame) misc_feature 3295..3297 /label=stop codon (-2 frame) misc_feature 3291..3293 /label=stop codon (-3 frame) misc_feature 3287..3298 /label=BBa_B0042 misc_feature 3307..3311 /label=Topoisomerase I recognition site misc_feature 3315..3319 /label=Nt.BstNB I recognition site misc_feature 3312..3314 /label=Arbitrary sequence (could be any bases) misc_feature 3311..3311 /label=Generated T overhang misc_feature 3307..3319 /label=BBa_B0043 promoter 3333..3351 /label=strong T7 promotor misc_feature 3351..3378 /label=lacO reg misc_feature 3328..3333 /label=scar of cloning into EcoRI site promoter 3328..3378 /label=BBa_K1362463 misc_feature 3387..3392 /label=EcoRI site misc_feature 3402..3407 /label=XbaI site misc_feature 3387..3408 /label=Prefix misc_feature 3393..3400 /label=NotI site misc_feature 3387..3408 /label=BBa_G00000 ORIGIN 1 tactagtagc ggccgctgca gtactagagg agtcactaag ggtactagag ttagttagtt 61 agtactagag attagcagaa agtcaaaagc ctccgaccgg aggcttttga ctaaaacttc 121 ccttggggtt atcattgggt actagaggct cactcaaagg cggtaattac tagagcagat 181 aaaaaaaatc cttagctttc gctaaggatg atttcttact agaggctaga gtactagagc 241 tgtcagacca agtttacgag ctcgcttgga ctcctgttga tagatccagt aatgacctca 301 gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtgag 361 aatccaagca ctagggacag taagacgggt aagcctgttg atgataccgc tgccttactg 421 ggtgcattag ccagtctgaa tgacctgtca cgggataatc cgaagtggtc agactggaaa 481 atcagagggc aggaactgct gaacagcaaa aagtcagata gcaccacata gcagacccgc 541 cataaaacgc cctgagaagc ccgtgacggg cttttcttgt attatgggta gtttccttgc 601 atgaatccat aaaaggcgcc tgtagtgcca tttaccccca ttcactgcca gagccgtgag 661 cgcagcgaac tgaatgtcac gaaaaagaca gcgactcagg tgcctgatgg tcggagacaa 721 aaggaatatt cagcgatttg cccgagcttg cgagggtgct acttaagcct ttagggtttt 781 aaggtctgtt ttgtagagga gcaaacagcg tttgcgacat ccttttgtaa tactgcggaa 841 ctgactaaag tagtgagtta tacacagggc tgggatctat tctttttatc tttttttatt 901 ctttctttat tctataaatt ataaccactt gaatataaac aaaaaaaaca cacaaaggtc 961 tagcggaatt tacagagggt ctagcagaat ttacaagttt tccagcaaag gtctagcaga 1021 atttacagat acccacaact caaaggaaaa ggacatgtaa ttatcattga ctagcccatc 1081 tcaattggta tagtgattaa aatcacctag accaattgag atgtatgtct gaattagttg 1141 ttttcaaagc aaatgaacta gcgattagtc gctatgactt aacggagcat gaaaccaagc 1201 taattttatg ctgtgtggca ctactcaacc ccacgattga aaaccctaca aggaaagaac 1261 ggacggtatc gttcacttat aaccaatacg ctcagatgat gaacatcagt agggaaaatg 1321 cttatggtgt attagctaaa gcaaccagag agctgatgac gagaactgtg gaaatcagga 1381 atcctttggt taaaggcttt gagattttcc agtggacaaa ctatgccaag ttctcaagcg 1441 aaaaattaga attagttttt agtgaagaga tattgcctta tcttttccag ttaaaaaaat 1501 tcataaaata taatctggaa catgttaagt cttttgaaaa caaatactct atgaggattt 1561 atgagtggtt attaaaagaa ctaacacaaa agaaaactca caaggcaaat atagagatta 1621 gccttgatga atttaagttc atgttaatgc ttgaaaataa ctaccatgag tttaaaaggc 1681 ttaaccaatg ggttttgaaa ccaataagta aagatttaaa cacttacagc aatatgaaat 1741 tggtggttga taagcgaggc cgcccgactg atacgttgat tttccaagtt gaactagata 1801 gacaaatgga tctcgtaacc gaacttgaga acaaccagat aaaaatgaat ggtgacaaaa 1861 taccaacaac cattacatca gattcctacc tacataacgg actaagaaaa acactacacg 1921 atgctttaac tgcaaaaatt cagctcacca gttttgaggc aaaatttttg agtgacatgc 1981 aaagtaagta tgatctcaat ggttcgttct catggctcac gcaaaaacaa cgaaccacac 2041 tagagaacat actggctaaa tacggaagga tctgaggttc ttatggctct tgtatctatc 2101 agtgaagcat caagactaac aaacaaaagt agaacaactg ttcaccgtta catatcaaag 2161 ggaaaactgt ccatatgcac agatgaaaac ggtgtaaaaa agatagatac atcagagctt 2221 ttacgagttt ttggtgcatt caaagctgtt caccatgaac agatcgacaa tgtaactact 2281 agagtactag agtactagag gttgatcggg cacgtaagag gttccaactt tcaccataat 2341 gaaataagat cactaccggg cgtatttttt gagttatcga gattttcagg agctaaggaa 2401 gctaaaatgg agaaaaaaat cacgggatat accaccgttg atatatccca atggcatcgt 2461 aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag 2521 ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc 2581 tttattcaca ttcttgcccg cctgatgaac gctcacccgg agtttcgtat ggccatgaaa 2641 gacggtgagc tggtgatctg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa 2701 actgaaacgt tttcgtccct ctggagtgaa taccacgacg atttccggca gtttctccac 2761 atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt 2821 attgagaata tgttttttgt ctcagccaat ccctgggtga gtttcaccag ttttgattta 2881 aacgtggcca atatggacaa cttcttcgcc cccgttttca cgatgggcaa atattatacg 2941 caaggcgaca aggtgctgat gccgctggcg atccaggttc atcatgccgt ttgtgatggc 3001 ttccatgtcg gccgcatgct taatgaatta caacagtact gtgatgagtg gcagggcggg 3061 gcgtaataat actagagtac tagctactag agtccggcaa aaaaacgggc aaggtgtcac 3121 caccctgccc tttttcttta aaaccgaaaa gattacttcg cgtttactag agtgccacct 3181 gacgtctaag aatactagag aaggaatatt cagcaatttg cccgtgccga agaaaggccc 3241 acccgtgaag gtgagccagt gagttgattg ctacgtaata ctagagttag ttagttagta 3301 ctagagccct tagtgactct actagaggaa tttaatacga ctcactatag gggaattgtg 3361 agcggataac aattccccta ctagaggaat tcgcggccgc ttctagag //