BBa_G00102 1 VR Reverse BioBrick primer annealing site (VR binding site) 2006-02-03T12:00:00Z 2015-08-31T04:07:28Z This is the VR sequence to be used in construction of plasmids which include the VR primer site. It is the reverse complement of BBa_G00101. false false _41_6_ 0 126 45 Not in stock false false Reshma Shetty annotation1961229 1 VR range1961229 1 1 20 BBa_G0003 1 scar NheI/XbaI mixed site 2007-02-26T12:00:00Z 2015-08-31T04:07:27Z NheI and XbaI NheI/XbaI mixed site false false _41_ 0 126 70 Not in stock false None. false Reshma Shetty BBa_G0002 1 SX scar SpeI/XbaI mixed site 2007-02-26T12:00:00Z 2015-08-31T04:07:27Z XbaI and SpeI sites XbaI/SpeI mixed site. Simply used to aid in entry of parts into the registry. false true _41_ 0 126 70 Not in stock false None. false Reshma Shetty BBa_B0062 1 BBa_B0062 Terminator (Reverse B0052) 2004-01-29T12:00:00Z 2015-08-31T04:07:21Z Reverse of B0052, E.coli transcriptional terminator from the ribosomal RNA rrnC operon. false false _1_ 0 24 7 Not in stock false false Caitlin Conboy annotation318637 1 stem_loop range318637 1 14 33 BBa_B0044 1 TOPO Topoisomerase I mediated cloning site (reverse) 2006-02-04T12:00:00Z 2015-08-31T04:07:20Z Insertion of this site will enable generation of a TOPO vector. The vector will have a T overhang and permit binding of topoisomerase I for direct cloning of PCR products. See http://openwetware.org/wiki/Topoisomerase_I_mediated_TA_cloning for a complete description of Topoisomerase I mediated cloning. This site belongs 3' of the cloning site. It is the reverse complement of BBa_B0043. false false _41_6_ 0 126 45 Not in stock false false Reshma Shetty annotation1797128 1 Topoisomerase I recognition site range1797128 1 9 13 annotation1797126 1 Nt.BstNB I recognition site range1797126 1 1 5 annotation1797127 1 Arbitrary sequence (could be any bases) range1797127 1 6 8 annotation1797129 1 Generated T overhang range1797129 1 9 9 BBa_G00001 1 Suffix BioBrick cloning site suffix 2006-03-13T12:00:00Z 2015-08-31T04:07:27Z This part is the standard BioBricks suffix. false true _41_6_ 0 126 45 Not in stock false false Reshma Shetty annotation1934226 1 SpeI range1934226 1 2 7 annotation1821544 1 Suffix range1821544 1 1 21 annotation1934227 1 PstI range1934227 1 16 21 annotation1934228 1 NotI range1934228 1 9 16 BBa_B0055 1 BBa_B0055 -- No description -- 2005-11-16T12:00:00Z 2015-08-31T04:07:20Z Terminator to be placed upstream of multiple cloning site in new pSB series plasmids. false false _41_6_ 0 126 44 Not in stock false false Reshma Shetty annotation1783301 1 stem loop from bacteriophage T3 range1783301 1 36 57 annotation1783311 1 identical to bacteriophage T3 range1783311 1 28 66 BBa_G00000 1 Prefix BioBrick cloning site prefix 2006-03-13T12:00:00Z 2015-08-31T04:07:27Z This part is the standard BioBricks prefix. false true _41_6_ 0 126 45 Not in stock false false Reshma Shetty annotation1961228 1 NotI site range1961228 1 7 14 annotation1934224 1 XbaI site range1934224 1 16 21 annotation1934225 1 Prefix range1934225 1 1 22 annotation1934223 1 EcoRI site range1934223 1 1 6 BBa_B0043 1 TOPO Topoisomerase I mediated cloning site (forward) 2006-02-04T12:00:00Z 2015-08-31T04:07:20Z Insertion of this site will enable generation of a TOPO vector. The vector will have a T overhang and permit binding of topoisomerase I for direct cloning of PCR products. See http://openwetware.org/wiki/Topoisomerase_I_mediated_TA_cloning for a complete description of Topoisomerase I mediated cloning. false false _41_6_ 0 126 45 Not in stock false false Reshma Shetty annotation1797101 1 Topoisomerase I recognition site range1797101 1 1 5 annotation1797103 1 Arbitrary sequence (could be any bases) range1797103 1 6 8 annotation1797102 1 Nt.BstNB I recognition site range1797102 1 9 13 annotation1797104 1 Generated T overhang range1797104 1 5 5 BBa_K1362096 1 pSB4C50 pSB4C50: Low(?) copy BioBrick cloning/expression backbone carrying Cm resistance 2014-10-07T11:00:00Z 2015-05-08T01:10:04Z false false _1738_ 0 22830 9 Not in stock false false Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha component2409927 1 BBa_K1362463 component2409864 1 BBa_B0042 component2409857 1 BBa_B0044 component2409871 1 BBa_B0062 component2409901 1 BBa_G0002 component2409932 1 BBa_G00000 component2409904 1 BBa_G0004 component2409900 1 BBa_I50042 component2409918 1 BBa_B0042 component2409911 1 BBa_B0055 component2409906 1 BBa_B0053 component2409923 1 BBa_B0043 component2409852 1 BBa_G00001 component2409903 1 BBa_P1004 component2409908 1 BBa_G00100 component2409872 1 BBa_G0003 component2409869 1 BBa_G00102 component2409867 1 BBa_B0054 annotation2409901 1 BBa_G0002 range2409901 1 2285 2292 annotation2409872 1 BBa_G0003 range2409872 1 225 231 annotation2409904 1 BBa_G0004 range2409904 1 3078 3084 annotation2409869 1 BBa_G00102 range2409869 1 148 167 annotation2409906 1 BBa_B0053 range2409906 1 3093 3164 annotation2409911 1 BBa_B0055 range2409911 1 3201 3278 annotation2409927 1 BBa_K1362463 range2409927 1 3328 3378 annotation2409852 1 BBa_G00001 range2409852 1 1 21 annotation2409918 1 BBa_B0042 range2409918 1 3287 3298 annotation2409867 1 BBa_B0054 range2409867 1 71 139 annotation2409908 1 BBa_G00100 range2409908 1 3173 3192 annotation2409871 1 BBa_B0062 range2409871 1 176 216 annotation2409932 1 BBa_G00000 range2409932 1 3387 3408 annotation2409864 1 BBa_B0042 range2409864 1 51 62 annotation2409903 1 BBa_P1004 range2409903 1 2301 3069 annotation2409900 1 BBa_I50042 range2409900 1 240 2276 annotation2409923 1 BBa_B0043 range2409923 1 3307 3319 annotation2409857 1 BBa_B0044 range2409857 1 30 42 BBa_G0004 1 scar SpeI/NheI mixed site 2007-02-26T12:00:00Z 2015-08-31T04:07:27Z NheI and SpeI NheI/SpeI mixed site. Used to help enter parts into the registry. false false _41_ 0 126 70 Not in stock false None. false Reshma Shetty BBa_P1004 1 cmR chloramphenicol resistance cassette 2006-02-03T12:00:00Z 2015-05-08T01:14:11Z Released HQ 2013 same part as BBa_P1002 except in reverse orientation. false false _41_6_ 0 126 45 In stock false true Reshma Shetty annotation1897114 1 cmR range1897114 1 107 769 BBa_B0054 1 BBa_B0054 Transcriptional terminator 2005-11-16T12:00:00Z 2015-08-31T04:07:20Z Terminator to be placed downstream of multiple cloning site in new pSB series plasmids. Similar to BBa_B0051. false false _41_6_ 0 126 44 Not in stock false false Reshma Shetty annotation1747359 1 stem loop range1747359 1 11 42 annotation1783342 1 identical to E. coli MG1655 between tonB and yciA range1783342 1 4 43 BBa_K1362463 1 T7/lac pro strong T7 promoter + LacO Regulator 2014-10-07T11:00:00Z 2015-05-08T01:10:06Z GAATTTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCC false false _1738_ 0 22830 9 Not in stock false false Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha annotation2405286 1 scar of cloning into EcoRI site range2405286 1 1 6 annotation2405276 1 lacO reg range2405276 1 24 51 annotation2405261 1 strong T7 promotor range2405261 1 6 24 BBa_B0042 1 TSS Translational stop sequence 2006-02-04T12:00:00Z 2015-08-31T04:07:20Z This part contains a stop codon in all 6 reading frames. Useful for ensuring that translation does not proceed through this part. false false _41_6_ 0 126 45 Not in stock false false Reshma Shetty annotation1797084 1 stop codon (-1 frame) range1797084 1 1 3 annotation1797088 1 stop codon (-3 frame) range1797088 1 5 7 annotation1797087 1 stop codon (-2 frame) range1797087 1 9 11 annotation1797083 1 stop codon (+2 frame) range1797083 1 2 4 annotation1797085 1 stop codon (+3 frame) range1797085 1 6 8 annotation1797086 1 stop codon (+1 frame) range1797086 1 10 12 BBa_B0053 1 BBa_B0053 Terminator (His) 2004-01-29T12:00:00Z 2015-08-31T04:07:20Z Terminator from the E.coli His operon false true _1_ 0 24 7 Not in stock false false Caitlin Conboy annotation318602 1 stem_loop range318602 1 9 43 BBa_I50042 1 pSC101 pSC101 origin of replication 2007-04-10T11:00:00Z 2015-08-31T04:07:42Z This part was amplified from pSB4A3-P1010. pSC101 origin of replication which is supposed to have low copy number. This was the origin used in Michael Elowitz's repressilator and in Bujard's pZS4Int-1 vector with mutations introduced to eliminate a SpeI site. It is also the origin of replication in pSB4* series plasmids. false false _41_ 0 126 70 Not in stock false The replication origin boundaries were chosen quite liberally (i.e. they might include more sequence than is strictly necessary) because a different version of this origin (BBa_I50040) was shown to be non-functional. false Reshma Shetty annotation2071313 1 inverted repeat range2071313 1 165 181 annotation2071310 1 par region range2071310 1 137 504 annotation2071311 1 inverted repeat range2071311 1 139 152 annotation2071325 1 inverted repeat range2071325 1 862 884 annotation2071321 1 -35 range2071321 1 828 833 annotation2071309 1 Terminator range2071309 1 46 75 annotation2071332 1 G to C difference with canonical pSC101 vector sequence range2071332 1 1912 1912 annotation2071315 1 inverted repeat range2071315 1 221 238 annotation2071329 1 G to A difference with canonical pSC101 vector sequence range2071329 1 1655 1655 annotation2071330 1 C to T difference with canonical pSC101 vector sequence range2071330 1 1750 1750 annotation2071326 1 RBS range2071326 1 870 882 annotation2071323 1 promoter region range2071323 1 846 865 annotation2071324 1 -10 range2071324 1 850 855 annotation2071327 1 RBS range2071327 1 879 884 annotation2071320 1 mutated SpeI site range2071320 1 814 819 annotation2071318 1 direct repeat range2071318 1 737 756 annotation2071317 1 direct repeat range2071317 1 716 735 annotation2071333 1 putative polyA range2071333 1 1947 1962 annotation2071319 1 direct repeat range2071319 1 769 788 annotation2071312 1 Tn3 integration site range2071312 1 158 162 annotation2071316 1 AT rich region range2071316 1 635 717 annotation2071328 1 repA range2071328 1 886 1836 annotation2071331 1 inverted repeat range2071331 1 1847 1861 annotation2071335 1 T to C difference with canonical pSC101 vector sequence range2071335 1 2002 2002 annotation2071334 1 inverted repeat range2071334 1 1963 1979 annotation2071314 1 inverted repeat range2071314 1 192 215 annotation2071322 1 inverted repeat range2071322 1 838 859 BBa_G00100 1 VF2 Forward primer for sequencing/amplifying BioBrick parts (VF2) 2004-05-24T11:00:00Z 2015-08-31T04:07:28Z Originally named VF2, primer binds upstream of the standard BBa_MCS on biobrick vectors. false false _11_1_ 0 60 7 Not in stock false false Tom Knight annotation1934505 1 VF2 range1934505 1 1 20 BBa_K1362096_sequence 1 tactagtagcggccgctgcagtactagaggagtcactaagggtactagagttagttagttagtactagagattagcagaaagtcaaaagcctccgaccggaggcttttgactaaaacttcccttggggttatcattgggtactagaggctcactcaaaggcggtaattactagagcagataaaaaaaatccttagctttcgctaaggatgatttcttactagaggctagagtactagagctgtcagaccaagtttacgagctcgcttggactcctgttgatagatccagtaatgacctcagaactccatctggatttgttcagaacgctcggttgccgccgggcgttttttattggtgagaatccaagcactagggacagtaagacgggtaagcctgttgatgataccgctgccttactgggtgcattagccagtctgaatgacctgtcacgggataatccgaagtggtcagactggaaaatcagagggcaggaactgctgaacagcaaaaagtcagatagcaccacatagcagacccgccataaaacgccctgagaagcccgtgacgggcttttcttgtattatgggtagtttccttgcatgaatccataaaaggcgcctgtagtgccatttacccccattcactgccagagccgtgagcgcagcgaactgaatgtcacgaaaaagacagcgactcaggtgcctgatggtcggagacaaaaggaatattcagcgatttgcccgagcttgcgagggtgctacttaagcctttagggttttaaggtctgttttgtagaggagcaaacagcgtttgcgacatccttttgtaatactgcggaactgactaaagtagtgagttatacacagggctgggatctattctttttatctttttttattctttctttattctataaattataaccacttgaatataaacaaaaaaaacacacaaaggtctagcggaatttacagagggtctagcagaatttacaagttttccagcaaaggtctagcagaatttacagatacccacaactcaaaggaaaaggacatgtaattatcattgactagcccatctcaattggtatagtgattaaaatcacctagaccaattgagatgtatgtctgaattagttgttttcaaagcaaatgaactagcgattagtcgctatgacttaacggagcatgaaaccaagctaattttatgctgtgtggcactactcaaccccacgattgaaaaccctacaaggaaagaacggacggtatcgttcacttataaccaatacgctcagatgatgaacatcagtagggaaaatgcttatggtgtattagctaaagcaaccagagagctgatgacgagaactgtggaaatcaggaatcctttggttaaaggctttgagattttccagtggacaaactatgccaagttctcaagcgaaaaattagaattagtttttagtgaagagatattgccttatcttttccagttaaaaaaattcataaaatataatctggaacatgttaagtcttttgaaaacaaatactctatgaggatttatgagtggttattaaaagaactaacacaaaagaaaactcacaaggcaaatatagagattagccttgatgaatttaagttcatgttaatgcttgaaaataactaccatgagtttaaaaggcttaaccaatgggttttgaaaccaataagtaaagatttaaacacttacagcaatatgaaattggtggttgataagcgaggccgcccgactgatacgttgattttccaagttgaactagatagacaaatggatctcgtaaccgaacttgagaacaaccagataaaaatgaatggtgacaaaataccaacaaccattacatcagattcctacctacataacggactaagaaaaacactacacgatgctttaactgcaaaaattcagctcaccagttttgaggcaaaatttttgagtgacatgcaaagtaagtatgatctcaatggttcgttctcatggctcacgcaaaaacaacgaaccacactagagaacatactggctaaatacggaaggatctgaggttcttatggctcttgtatctatcagtgaagcatcaagactaacaaacaaaagtagaacaactgttcaccgttacatatcaaagggaaaactgtccatatgcacagatgaaaacggtgtaaaaaagatagatacatcagagcttttacgagtttttggtgcattcaaagctgttcaccatgaacagatcgacaatgtaactactagagtactagagtactagaggttgatcgggcacgtaagaggttccaactttcaccataatgaaataagatcactaccgggcgtattttttgagttatcgagattttcaggagctaaggaagctaaaatggagaaaaaaatcacgggatataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgctcaatgtacctataaccagaccgttcagctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatgaacgctcacccggagtttcgtatggccatgaaagacggtgagctggtgatctgggatagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcgtccctctggagtgaataccacgacgatttccggcagtttctccacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgttttttgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcacgatgggcaaatattatacgcaaggcgacaaggtgctgatgccgctggcgatccaggttcatcatgccgtttgtgatggcttccatgtcggccgcatgcttaatgaattacaacagtactgtgatgagtggcagggcggggcgtaataatactagagtactagctactagagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttactagagtgccacctgacgtctaagaatactagagaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaatactagagttagttagttagtactagagcccttagtgactctactagaggaatttaatacgactcactataggggaattgtgagcggataacaattcccctactagaggaattcgcggccgcttctagag BBa_G0004_sequence 1 tactagc BBa_P1004_sequence 1 gttgatcgggcacgtaagaggttccaactttcaccataatgaaataagatcactaccgggcgtattttttgagttatcgagattttcaggagctaaggaagctaaaatggagaaaaaaatcacgggatataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgctcaatgtacctataaccagaccgttcagctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatgaacgctcacccggagtttcgtatggccatgaaagacggtgagctggtgatctgggatagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcgtccctctggagtgaataccacgacgatttccggcagtttctccacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgttttttgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcacgatgggcaaatattatacgcaaggcgacaaggtgctgatgccgctggcgatccaggttcatcatgccgtttgtgatggcttccatgtcggccgcatgcttaatgaattacaacagtactgtgatgagtggcagggcggggcgtaataa BBa_B0062_sequence 1 cagataaaaaaaatccttagctttcgctaaggatgatttct BBa_B0055_sequence 1 aaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaa BBa_B0044_sequence 1 gagtcactaaggg BBa_B0042_sequence 1 ttagttagttag BBa_G0003_sequence 1 gctagag BBa_B0054_sequence 1 attagcagaaagtcaaaagcctccgaccggaggcttttgactaaaacttcccttggggttatcattggg BBa_G00000_sequence 1 gaattcgcggccgcttctagag BBa_G00001_sequence 1 tactagtagcggccgctgcag BBa_B0053_sequence 1 tccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtt BBa_I50042_sequence 1 ctgtcagaccaagtttacgagctcgcttggactcctgttgatagatccagtaatgacctcagaactccatctggatttgttcagaacgctcggttgccgccgggcgttttttattggtgagaatccaagcactagggacagtaagacgggtaagcctgttgatgataccgctgccttactgggtgcattagccagtctgaatgacctgtcacgggataatccgaagtggtcagactggaaaatcagagggcaggaactgctgaacagcaaaaagtcagatagcaccacatagcagacccgccataaaacgccctgagaagcccgtgacgggcttttcttgtattatgggtagtttccttgcatgaatccataaaaggcgcctgtagtgccatttacccccattcactgccagagccgtgagcgcagcgaactgaatgtcacgaaaaagacagcgactcaggtgcctgatggtcggagacaaaaggaatattcagcgatttgcccgagcttgcgagggtgctacttaagcctttagggttttaaggtctgttttgtagaggagcaaacagcgtttgcgacatccttttgtaatactgcggaactgactaaagtagtgagttatacacagggctgggatctattctttttatctttttttattctttctttattctataaattataaccacttgaatataaacaaaaaaaacacacaaaggtctagcggaatttacagagggtctagcagaatttacaagttttccagcaaaggtctagcagaatttacagatacccacaactcaaaggaaaaggacatgtaattatcattgactagcccatctcaattggtatagtgattaaaatcacctagaccaattgagatgtatgtctgaattagttgttttcaaagcaaatgaactagcgattagtcgctatgacttaacggagcatgaaaccaagctaattttatgctgtgtggcactactcaaccccacgattgaaaaccctacaaggaaagaacggacggtatcgttcacttataaccaatacgctcagatgatgaacatcagtagggaaaatgcttatggtgtattagctaaagcaaccagagagctgatgacgagaactgtggaaatcaggaatcctttggttaaaggctttgagattttccagtggacaaactatgccaagttctcaagcgaaaaattagaattagtttttagtgaagagatattgccttatcttttccagttaaaaaaattcataaaatataatctggaacatgttaagtcttttgaaaacaaatactctatgaggatttatgagtggttattaaaagaactaacacaaaagaaaactcacaaggcaaatatagagattagccttgatgaatttaagttcatgttaatgcttgaaaataactaccatgagtttaaaaggcttaaccaatgggttttgaaaccaataagtaaagatttaaacacttacagcaatatgaaattggtggttgataagcgaggccgcccgactgatacgttgattttccaagttgaactagatagacaaatggatctcgtaaccgaacttgagaacaaccagataaaaatgaatggtgacaaaataccaacaaccattacatcagattcctacctacataacggactaagaaaaacactacacgatgctttaactgcaaaaattcagctcaccagttttgaggcaaaatttttgagtgacatgcaaagtaagtatgatctcaatggttcgttctcatggctcacgcaaaaacaacgaaccacactagagaacatactggctaaatacggaaggatctgaggttcttatggctcttgtatctatcagtgaagcatcaagactaacaaacaaaagtagaacaactgttcaccgttacatatcaaagggaaaactgtccatatgcacagatgaaaacggtgtaaaaaagatagatacatcagagcttttacgagtttttggtgcattcaaagctgttcaccatgaacagatcgacaatgtaac BBa_G00100_sequence 1 tgccacctgacgtctaagaa BBa_K1362463_sequence 1 gaatttaatacgactcactataggggaattgtgagcggataacaattcccc BBa_G0002_sequence 1 tactagag BBa_G00102_sequence 1 gctcactcaaaggcggtaat BBa_B0043_sequence 1 cccttagtgactc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z