##gff-version 3
##sequence-region BBa_K1362096 1 3408
BBa_K1362096	.	sequence_feature	1	21	.	+	0	ID=BBa_G00001;Name=BBa_G00001
BBa_K1362096	.	engineered_region	1	21	.	+	0	ID=annotation1821544;Name=Suffix;Parent=BBa_G00001
BBa_K1362096	.	sequence_feature	16	21	.	+	0	ID=annotation1934227;Name=PstI;Parent=BBa_G00001
BBa_K1362096	.	sequence_feature	9	16	.	+	0	ID=annotation1934228;Name=NotI;Parent=BBa_G00001
BBa_K1362096	.	sequence_feature	2	7	.	+	0	ID=annotation1934226;Name=SpeI;Parent=BBa_G00001
BBa_K1362096	.	sequence_feature	3387	3408	.	+	0	ID=BBa_G00000;Name=BBa_G00000
BBa_K1362096	.	engineered_region	3387	3408	.	+	0	ID=annotation1934225;Name=Prefix;Parent=BBa_G00000
BBa_K1362096	.	sequence_feature	3387	3392	.	+	0	ID=annotation1934223;Name=EcoRI site;Parent=BBa_G00000
BBa_K1362096	.	sequence_feature	3402	3407	.	+	0	ID=annotation1934224;Name=XbaI site;Parent=BBa_G00000
BBa_K1362096	.	sequence_feature	3393	3400	.	+	0	ID=annotation1961228;Name=NotI site;Parent=BBa_G00000
BBa_K1362096	.	sequence_feature	3307	3319	.	+	0	ID=BBa_B0043;Name=BBa_B0043
BBa_K1362096	.	sequence_feature	3311	3311	.	+	0	ID=annotation1797104;Name=Generated T overhang;Parent=BBa_B0043
BBa_K1362096	.	sequence_feature	3307	3311	.	+	0	ID=annotation1797101;Name=Topoisomerase I recognition site;Parent=BBa_B0043
BBa_K1362096	.	sequence_feature	3312	3314	.	+	0	ID=annotation1797103;Name=Arbitrary sequence (could be any bases);Parent=BBa_B0043
BBa_K1362096	.	sequence_feature	3315	3319	.	+	0	ID=annotation1797102;Name=Nt.BstNB I recognition site;Parent=BBa_B0043
BBa_K1362096	.	terminator	176	216	.	+	0	ID=BBa_B0062;Name=BBa_B0062
BBa_K1362096	.	stem_loop	189	208	.	+	0	ID=annotation318637;Name=stem_loop;Parent=BBa_B0062
BBa_K1362096	.	sequence_feature	240	2276	.	+	0	ID=BBa_I50042;Name=BBa_I50042
BBa_K1362096	.	ribosome_entry_site	1109	1121	.	+	0	ID=annotation2071326;Name=RBS;Parent=BBa_I50042
BBa_K1362096	.	sequence_feature	1008	1027	.	+	0	ID=annotation2071319;Name=direct repeat;Parent=BBa_I50042
BBa_K1362096	.	promoter	1085	1104	.	+	0	ID=annotation2071323;Name=promoter region;Parent=BBa_I50042
BBa_K1362096	.	sequence_feature	431	454	.	+	0	ID=annotation2071314;Name=inverted repeat;Parent=BBa_I50042
BBa_K1362096	.	sequence_feature	1101	1123	.	+	0	ID=annotation2071325;Name=inverted repeat;Parent=BBa_I50042
BBa_K1362096	.	promoter	1089	1094	.	+	0	ID=annotation2071324;Name=-10;Parent=BBa_I50042
BBa_K1362096	.	sequence_alteration	2151	2151	.	+	0	ID=annotation2071332;Name=G to C difference with canonical pSC101 vector sequence;Parent=BBa_I50042
BBa_K1362096	.	sequence_feature	1077	1098	.	+	0	ID=annotation2071322;Name=inverted repeat;Parent=BBa_I50042
BBa_K1362096	.	sequence_alteration	2241	2241	.	+	0	ID=annotation2071335;Name=T to C difference with canonical pSC101 vector sequence;Parent=BBa_I50042
BBa_K1362096	.	sequence_feature	874	956	.	+	0	ID=annotation2071316;Name=AT rich region;Parent=BBa_I50042
BBa_K1362096	.	polyA_site	2186	2201	.	+	0	ID=annotation2071333;Name=putative polyA;Parent=BBa_I50042
BBa_K1362096	.	sequence_feature	397	401	.	+	0	ID=annotation2071312;Name=Tn3 integration site;Parent=BBa_I50042
BBa_K1362096	.	sequence_feature	976	995	.	+	0	ID=annotation2071318;Name=direct repeat;Parent=BBa_I50042
BBa_K1362096	.	sequence_alteration	1989	1989	.	+	0	ID=annotation2071330;Name=C to T difference with canonical pSC101 vector sequence;Parent=BBa_I50042
BBa_K1362096	.	sequence_alteration	1894	1894	.	+	0	ID=annotation2071329;Name=G to A difference with canonical pSC101 vector sequence;Parent=BBa_I50042
BBa_K1362096	.	CDS	1125	2075	.	+	0	ID=annotation2071328;Name=repA;Parent=BBa_I50042
BBa_K1362096	.	stem_loop	285	314	.	+	0	ID=annotation2071309;Name=Terminator;Parent=BBa_I50042
BBa_K1362096	.	sequence_feature	2202	2218	.	+	0	ID=annotation2071334;Name=inverted repeat;Parent=BBa_I50042
BBa_K1362096	.	sequence_feature	460	477	.	+	0	ID=annotation2071315;Name=inverted repeat;Parent=BBa_I50042
BBa_K1362096	.	sequence_alteration	1053	1058	.	+	0	ID=annotation2071320;Name=mutated SpeI site;Parent=BBa_I50042
BBa_K1362096	.	ribosome_entry_site	1118	1123	.	+	0	ID=annotation2071327;Name=RBS;Parent=BBa_I50042
BBa_K1362096	.	sequence_feature	955	974	.	+	0	ID=annotation2071317;Name=direct repeat;Parent=BBa_I50042
BBa_K1362096	.	sequence_feature	2086	2100	.	+	0	ID=annotation2071331;Name=inverted repeat;Parent=BBa_I50042
BBa_K1362096	.	sequence_feature	378	391	.	+	0	ID=annotation2071311;Name=inverted repeat;Parent=BBa_I50042
BBa_K1362096	.	promoter	1067	1072	.	+	0	ID=annotation2071321;Name=-35;Parent=BBa_I50042
BBa_K1362096	.	sequence_feature	404	420	.	+	0	ID=annotation2071313;Name=inverted repeat;Parent=BBa_I50042
BBa_K1362096	.	sequence_feature	376	743	.	+	0	ID=annotation2071310;Name=par region;Parent=BBa_I50042
BBa_K1362096	.	sequence_feature	3287	3298	.	+	0	ID=BBa_B0042;Name=BBa_B0042
BBa_K1362096	.	stop_codon	3292	3294	.	+	0	ID=annotation1797085;Name=stop codon (+3 frame);Parent=BBa_B0042
BBa_K1362096	.	stop_codon	3287	3289	.	+	0	ID=annotation1797084;Name=stop codon (-1 frame);Parent=BBa_B0042
BBa_K1362096	.	stop_codon	3288	3290	.	+	0	ID=annotation1797083;Name=stop codon (+2 frame);Parent=BBa_B0042
BBa_K1362096	.	stop_codon	3296	3298	.	+	0	ID=annotation1797086;Name=stop codon (+1 frame);Parent=BBa_B0042
BBa_K1362096	.	stop_codon	3295	3297	.	+	0	ID=annotation1797087;Name=stop codon (-2 frame);Parent=BBa_B0042
BBa_K1362096	.	stop_codon	3291	3293	.	+	0	ID=annotation1797088;Name=stop codon (-3 frame);Parent=BBa_B0042
BBa_K1362096	.	sequence_feature	30	42	.	+	0	ID=BBa_B0044;Name=BBa_B0044
BBa_K1362096	.	sequence_feature	38	38	.	+	0	ID=annotation1797129;Name=Generated T overhang;Parent=BBa_B0044
BBa_K1362096	.	sequence_feature	35	37	.	+	0	ID=annotation1797127;Name=Arbitrary sequence (could be any bases);Parent=BBa_B0044
BBa_K1362096	.	sequence_feature	38	42	.	+	0	ID=annotation1797128;Name=Topoisomerase I recognition site;Parent=BBa_B0044
BBa_K1362096	.	sequence_feature	30	34	.	+	0	ID=annotation1797126;Name=Nt.BstNB I recognition site;Parent=BBa_B0044
BBa_K1362096	.	terminator	3093	3164	.	+	0	ID=BBa_B0053;Name=BBa_B0053
BBa_K1362096	.	stem_loop	3101	3135	.	+	0	ID=annotation318602;Name=stem_loop;Parent=BBa_B0053
BBa_K1362096	.	sequence_feature	51	62	.	+	0	ID=BBa_B0042;Name=BBa_B0042
BBa_K1362096	.	stop_codon	56	58	.	+	0	ID=annotation1797085;Name=stop codon (+3 frame);Parent=BBa_B0042
BBa_K1362096	.	stop_codon	51	53	.	+	0	ID=annotation1797084;Name=stop codon (-1 frame);Parent=BBa_B0042
BBa_K1362096	.	stop_codon	52	54	.	+	0	ID=annotation1797083;Name=stop codon (+2 frame);Parent=BBa_B0042
BBa_K1362096	.	stop_codon	60	62	.	+	0	ID=annotation1797086;Name=stop codon (+1 frame);Parent=BBa_B0042
BBa_K1362096	.	stop_codon	59	61	.	+	0	ID=annotation1797087;Name=stop codon (-2 frame);Parent=BBa_B0042
BBa_K1362096	.	stop_codon	55	57	.	+	0	ID=annotation1797088;Name=stop codon (-3 frame);Parent=BBa_B0042
BBa_K1362096	.	sequence_feature	148	167	.	+	0	ID=BBa_G00102;Name=BBa_G00102
BBa_K1362096	.	primer_binding_site	148	167	.	+	0	ID=annotation1961229;Name=VR;Parent=BBa_G00102
BBa_K1362096	.	terminator	71	139	.	+	0	ID=BBa_B0054;Name=BBa_B0054
BBa_K1362096	.	sequence_feature	74	113	.	+	0	ID=annotation1783342;Name=identical to E. coli MG1655 between tonB and yciA;Parent=BBa_B0054
BBa_K1362096	.	stem_loop	81	112	.	+	0	ID=annotation1747359;Name=stem loop;Parent=BBa_B0054
BBa_K1362096	.	sequence_feature	3078	3084	.	+	0	ID=BBa_G0004;Name=BBa_G0004
BBa_K1362096	.	sequence_feature	225	231	.	+	0	ID=BBa_G0003;Name=BBa_G0003
BBa_K1362096	.	engineered_region	2301	3069	.	+	0	ID=BBa_P1004;Name=BBa_P1004
BBa_K1362096	.	CDS	2407	3069	.	+	0	ID=annotation1897114;Name=cmR;Parent=BBa_P1004
BBa_K1362096	.	sequence_feature	2285	2292	.	+	0	ID=BBa_G0002;Name=BBa_G0002
BBa_K1362096	.	promoter	3328	3378	.	+	0	ID=BBa_K1362463;Name=BBa_K1362463
BBa_K1362096	.	null	3328	3333	.	+	0	ID=annotation2405286;Name=scar of cloning into EcoRI site;Parent=BBa_K1362463
BBa_K1362096	.	operator	3351	3378	.	+	0	ID=annotation2405276;Name=lacO reg;Parent=BBa_K1362463
BBa_K1362096	.	promoter	3333	3351	.	+	0	ID=annotation2405261;Name=strong T7 promotor;Parent=BBa_K1362463
BBa_K1362096	.	terminator	3201	3278	.	+	0	ID=BBa_B0055;Name=BBa_B0055
BBa_K1362096	.	sequence_feature	3228	3266	.	+	0	ID=annotation1783311;Name=identical to bacteriophage T3;Parent=BBa_B0055
BBa_K1362096	.	stem_loop	3236	3257	.	+	0	ID=annotation1783301;Name=stem loop from bacteriophage T3;Parent=BBa_B0055
BBa_K1362096	.	primer	3173	3192	.	+	0	ID=BBa_G00100;Name=BBa_G00100
BBa_K1362096	.	primer_binding_site	3173	3192	.	+	0	ID=annotation1934505;Name=VF2;Parent=BBa_G00100
>BBa_K1362096
tactagtagcggccgctgcagtactagaggagtcactaagggtactagagttagttagttagtactagagattagcaga
aagtcaaaagcctccgaccggaggcttttgactaaaacttcccttggggttatcattgggtactagaggctcactcaaa
ggcggtaattactagagcagataaaaaaaatccttagctttcgctaaggatgatttcttactagaggctagagtactag
agctgtcagaccaagtttacgagctcgcttggactcctgttgatagatccagtaatgacctcagaactccatctggatt
tgttcagaacgctcggttgccgccgggcgttttttattggtgagaatccaagcactagggacagtaagacgggtaagcc
tgttgatgataccgctgccttactgggtgcattagccagtctgaatgacctgtcacgggataatccgaagtggtcagac
tggaaaatcagagggcaggaactgctgaacagcaaaaagtcagatagcaccacatagcagacccgccataaaacgccct
gagaagcccgtgacgggcttttcttgtattatgggtagtttccttgcatgaatccataaaaggcgcctgtagtgccatt
tacccccattcactgccagagccgtgagcgcagcgaactgaatgtcacgaaaaagacagcgactcaggtgcctgatggt
cggagacaaaaggaatattcagcgatttgcccgagcttgcgagggtgctacttaagcctttagggttttaaggtctgtt
ttgtagaggagcaaacagcgtttgcgacatccttttgtaatactgcggaactgactaaagtagtgagttatacacaggg
ctgggatctattctttttatctttttttattctttctttattctataaattataaccacttgaatataaacaaaaaaaa
cacacaaaggtctagcggaatttacagagggtctagcagaatttacaagttttccagcaaaggtctagcagaatttaca
gatacccacaactcaaaggaaaaggacatgtaattatcattgactagcccatctcaattggtatagtgattaaaatcac
ctagaccaattgagatgtatgtctgaattagttgttttcaaagcaaatgaactagcgattagtcgctatgacttaacgg
agcatgaaaccaagctaattttatgctgtgtggcactactcaaccccacgattgaaaaccctacaaggaaagaacggac
ggtatcgttcacttataaccaatacgctcagatgatgaacatcagtagggaaaatgcttatggtgtattagctaaagca
accagagagctgatgacgagaactgtggaaatcaggaatcctttggttaaaggctttgagattttccagtggacaaact
atgccaagttctcaagcgaaaaattagaattagtttttagtgaagagatattgccttatcttttccagttaaaaaaatt
cataaaatataatctggaacatgttaagtcttttgaaaacaaatactctatgaggatttatgagtggttattaaaagaa
ctaacacaaaagaaaactcacaaggcaaatatagagattagccttgatgaatttaagttcatgttaatgcttgaaaata
actaccatgagtttaaaaggcttaaccaatgggttttgaaaccaataagtaaagatttaaacacttacagcaatatgaa
attggtggttgataagcgaggccgcccgactgatacgttgattttccaagttgaactagatagacaaatggatctcgta
accgaacttgagaacaaccagataaaaatgaatggtgacaaaataccaacaaccattacatcagattcctacctacata
acggactaagaaaaacactacacgatgctttaactgcaaaaattcagctcaccagttttgaggcaaaatttttgagtga
catgcaaagtaagtatgatctcaatggttcgttctcatggctcacgcaaaaacaacgaaccacactagagaacatactg
gctaaatacggaaggatctgaggttcttatggctcttgtatctatcagtgaagcatcaagactaacaaacaaaagtaga
acaactgttcaccgttacatatcaaagggaaaactgtccatatgcacagatgaaaacggtgtaaaaaagatagatacat
cagagcttttacgagtttttggtgcattcaaagctgttcaccatgaacagatcgacaatgtaactactagagtactaga
gtactagaggttgatcgggcacgtaagaggttccaactttcaccataatgaaataagatcactaccgggcgtatttttt
gagttatcgagattttcaggagctaaggaagctaaaatggagaaaaaaatcacgggatataccaccgttgatatatccc
aatggcatcgtaaagaacattttgaggcatttcagtcagttgctcaatgtacctataaccagaccgttcagctggatat
tacggcctttttaaagaccgtaaagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatg
aacgctcacccggagtttcgtatggccatgaaagacggtgagctggtgatctgggatagtgttcacccttgttacaccg
ttttccatgagcaaactgaaacgttttcgtccctctggagtgaataccacgacgatttccggcagtttctccacatata
ttcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgttttttgtctca
gccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcacga
tgggcaaatattatacgcaaggcgacaaggtgctgatgccgctggcgatccaggttcatcatgccgtttgtgatggctt
ccatgtcggccgcatgcttaatgaattacaacagtactgtgatgagtggcagggcggggcgtaataatactagagtact
agctactagagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcg
cgtttactagagtgccacctgacgtctaagaatactagagaaggaatattcagcaatttgcccgtgccgaagaaaggcc
cacccgtgaaggtgagccagtgagttgattgctacgtaatactagagttagttagttagtactagagcccttagtgact
ctactagaggaatttaatacgactcactataggggaattgtgagcggataacaattcccctactagaggaattcgcggc
cgcttctagag