LOCUS BBa_K1362097 3470 bp DNA linear 31-Oct-2024 DEFINITION Low copy BioBrick expression backbone carrying Kan resistance ACCESSION BBa_K1362097 VERSION BBa_K1362097.1 FEATURES Location/Qualifiers misc_feature 1..21 /label=Suffix misc_feature 2..7 /label=SpeI misc_feature 16..21 /label=PstI misc_feature 9..16 /label=NotI misc_feature 1..21 /label=BBa_G00001 misc_feature 22..26 /label=Nt.BstNB I recognition site misc_feature 27..29 /label=Arbitrary sequence (could be any bases) misc_feature 30..34 /label=Topoisomerase I recognition site misc_feature 30..30 /label=Generated T overhang misc_feature 22..34 /label=BBa_B0044 misc_feature 36..38 /label=stop codon (+2 frame) misc_feature 35..37 /label=stop codon (-1 frame) misc_feature 40..42 /label=stop codon (+3 frame) misc_feature 44..46 /label=stop codon (+1 frame) misc_feature 43..45 /label=stop codon (-2 frame) misc_feature 39..41 /label=stop codon (-3 frame) misc_feature 35..46 /label=BBa_B0042 stem_loop 57..88 /label=stem loop misc_feature 50..89 /label=identical to E. coli MG1655 between tonB and yciA terminator 47..115 /label=BBa_B0054 primer_bind 116..135 /label=VR misc_feature 116..135 /label=BBa_G00102 stem_loop 149..168 /label=stem_loop terminator 136..176 /label=BBa_B0062 misc_feature 177..183 /label=BBa_G0005 CDS 184..1002 /label=kanamycin resistance misc_feature 970..970 /label=G to C mutation to eliminate XhoI site misc_feature 1107..1150 /label=repeat region misc_feature 184..1150 /label=BBa_P1007 misc_feature 1151..1158 /label=BBa_G0001 polyA_site 1234..1249 /label=putative polyA CDS 1360..2310 /label=repA misc_feature 2312..2334 /label=inverted repeat RBS 2312..2317 /label=RBS RBS 2314..2326 /label=RBS promoter 2331..2350 /label=promoter region misc_feature 2337..2358 /label=inverted repeat promoter 2341..2346 /label=rep promoter -10 promoter 2363..2368 /label=rep promoter -35 misc_feature 2377..2382 /label=Mutated SpeI site misc_feature 2408..2427 /label=direct repeat misc_feature 2440..2459 /label=direct repeat misc_feature 2461..2480 /label=direct repeat misc_feature 2479..2561 /label=AT rich region misc_feature 2692..3059 /label=par region misc_feature 2958..2975 /label=inverted repeat misc_feature 2981..3004 /label=inverted repeat misc_feature 3015..3031 /label=inverted repeat misc_feature 3034..3038 /label=Tn3 integration site misc_feature 3044..3057 /label=inverted repeat stem_loop 3121..3150 /label=terminator misc_feature 1194..1194 /label=difference from canonical pSC101 sequence in Genbank misc_feature 1284..1284 /label=difference from canonical pSC101 sequence in Genbank misc_feature 1446..1446 /label=difference from canonical pSC101 sequence in Genbank misc_feature 1541..1541 /label=difference from canonical pSC101 sequence in Genbank misc_feature 1159..3195 /label=BBa_I50041 misc_feature 3196..3202 /label=BBa_G0006 stem_loop 3211..3245 /label=stem_loop terminator 3203..3274 /label=BBa_B0053 primer_bind 3275..3294 /label=VF2 primer 3275..3294 /label=BBa_G00100 stem_loop 3330..3351 /label=stem loop from bacteriophage T3 misc_feature 3322..3360 /label=identical to bacteriophage T3 terminator 3295..3372 /label=BBa_B0055 misc_feature 3374..3376 /label=stop codon (+2 frame) misc_feature 3373..3375 /label=stop codon (-1 frame) misc_feature 3378..3380 /label=stop codon (+3 frame) misc_feature 3382..3384 /label=stop codon (+1 frame) misc_feature 3381..3383 /label=stop codon (-2 frame) misc_feature 3377..3379 /label=stop codon (-3 frame) misc_feature 3373..3384 /label=BBa_B0042 misc_feature 3385..3389 /label=Topoisomerase I recognition site misc_feature 3393..3397 /label=Nt.BstNB I recognition site misc_feature 3390..3392 /label=Arbitrary sequence (could be any bases) misc_feature 3389..3389 /label=Generated T overhang misc_feature 3385..3397 /label=BBa_B0043 promoter 3403..3421 /label=strong T7 promotor misc_feature 3421..3448 /label=lacO reg misc_feature 3398..3403 /label=scar of cloning into EcoRI site promoter 3398..3448 /label=BBa_K1362463 misc_feature 3449..3454 /label=EcoRI site misc_feature 3464..3469 /label=XbaI site misc_feature 3449..3470 /label=Prefix misc_feature 3455..3462 /label=NotI site misc_feature 3449..3470 /label=BBa_G00000 ORIGIN 1 tactagtagc ggccgctgca ggagtcacta agggttagtt agttagatta gcagaaagtc 61 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttggggctca 121 ctcaaaggcg gtaatcagat aaaaaaaatc cttagctttc gctaaggatg atttctgcta 181 gtattattag aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt 241 atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca 301 gttccaaaga atggcaaggt cctggtaacg gtctgcgatt ccgacccgtc caacatcaat 361 acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt 421 gacgactgaa tccggtgaga atggcaagag cttgtgcatt tctttccaga cttgttcaac 481 aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcatgcg 541 tgattgcgcc tgagcaagac gaaatacacg atcgctgtta aaaggacaat tacaaacagg 601 aatcgaatgt aaccggcgca ggaacacggc cagcgcatca acaatatttt cacctgaatc 661 aggatattct tctaatacct ggaaggctgt tttcccagga atcgcggtgg tgagtaacca 721 cgcatcatca ggagtacgga taaaatgctt gatggtcggg agaggcataa actccgtcag 781 ccagttgaga cggaccatct catctgtaac atcattggca acgctacctt tgccatgttt 841 cagaaacaac tctggcgcat cgggcttccc atacaagcga tagattgtcg cacctgattg 901 cccgacatta tcgcgagccc atttataccc atataaatca gcgtccatgt tggagtttaa 961 gcgcggacgg gagcaagacg tttcccgttg aatatggctc ataacacccc ttgtattact 1021 gtttatgtaa gcagacagtt ttattgttca tgatgatata tttttatctt gtgcaatgta 1081 acatcagaga ttttgagaca caacgtggct ttgttgaata aatcgaactt ttgctgagtt 1141 gaaggatcag ctctagtagt tacattgtcg atctgttcat ggtgaacagc tttgaatgca 1201 ccaaaaactc gtaaaagctc tgatgtatct atctttttta caccgttttc atctgtgcat 1261 atggacagtt ttccctttga tatgtaacgg tgaacagttg ttctactttt gtttgttagt 1321 cttgatgctt cactgataga tacaagagcc ataagaacct cagatccttc cgtatttagc 1381 cagtatgttc tctagtgtgg ttcgttgttt ttgcgtgagc catgagaacg aaccattgag 1441 atcatactta ctttgcatgt cactcaaaaa ttttgcctca aaactggtga gctgaatttt 1501 tgcagttaaa gcatcgtgta gtgtttttct tagtccgtta tgtaggtagg aatctgatgt 1561 aatggttgtt ggtattttgt caccattcat ttttatctgg ttgttctcaa gttcggttac 1621 gagatccatt tgtctatcta gttcaacttg gaaaatcaac gtatcagtcg ggcggcctcg 1681 cttatcaacc accaatttca tattgctgta agtgtttaaa tctttactta ttggtttcaa 1741 aacccattgg ttaagccttt taaactcatg gtagttattt tcaagcatta acatgaactt 1801 aaattcatca aggctaatct ctatatttgc cttgtgagtt ttcttttgtg ttagttcttt 1861 taataaccac tcataaatcc tcatagagta tttgttttca aaagacttaa catgttccag 1921 attatatttt atgaattttt ttaactggaa aagataaggc aatatctctt cactaaaaac 1981 taattctaat ttttcgcttg agaacttggc atagtttgtc cactggaaaa tctcaaagcc 2041 tttaaccaaa ggattcctga tttccacagt tctcgtcatc agctctctgg ttgctttagc 2101 taatacacca taagcatttt ccctactgat gttcatcatc tgagcgtatt ggttataagt 2161 gaacgatacc gtccgttctt tccttgtagg gttttcaatc gtggggttga gtagtgccac 2221 acagcataaa attagcttgg tttcatgctc cgttaagtca tagcgactaa tcgctagttc 2281 atttgctttg aaaacaacta attcagacat acatctcaat tggtctaggt gattttaatc 2341 actataccaa ttgagatggg ctagtcaatg ataattacat gtccttttcc tttgagttgt 2401 gggtatctgt aaattctgct agacctttgc tggaaaactt gtaaattctg ctagaccctc 2461 tgtaaattcc gctagacctt tgtgtgtttt ttttgtttat attcaagtgg ttataattta 2521 tagaataaag aaagaataaa aaaagataaa aagaatagat cccagccctg tgtataactc 2581 actactttag tcagttccgc agtattacaa aaggatgtcg caaacgctgt ttgctcctct 2641 acaaaacaga ccttaaaacc ctaaaggctt aagtagcacc ctcgcaagct cgggcaaatc 2701 gctgaatatt ccttttgtct ccgaccatca ggcacctgag tcgctgtctt tttcgtgaca 2761 ttcagttcgc tgcgctcacg gctctggcag tgaatggggg taaatggcac tacaggcgcc 2821 ttttatggat tcatgcaagg aaactaccca taatacaaga aaagcccgtc acgggcttct 2881 cagggcgttt tatggcgggt ctgctatgtg gtgctatctg actttttgct gttcagcagt 2941 tcctgccctc tgattttcca gtctgaccac ttcggattat cccgtgacag gtcattcaga 3001 ctggctaatg cacccagtaa ggcagcggta tcatcaacag gcttacccgt cttactgtcc 3061 ctagtgcttg gattctcacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa 3121 tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctcgta 3181 aacttggtct gacagctcta gctccggcaa aaaaacgggc aaggtgtcac caccctgccc 3241 tttttcttta aaaccgaaaa gattacttcg cgtttgccac ctgacgtcta agaaaaggaa 3301 tattcagcaa tttgcccgtg ccgaagaaag gcccacccgt gaaggtgagc cagtgagttg 3361 attgctacgt aattagttag ttagccctta gtgactcgaa tttaatacga ctcactatag 3421 gggaattgtg agcggataac aattccccga attcgcggcc gcttctagag //