BBa_B0054 1 BBa_B0054 Transcriptional terminator 2005-11-16T12:00:00Z 2015-08-31T04:07:20Z Terminator to be placed downstream of multiple cloning site in new pSB series plasmids. Similar to BBa_B0051. false false _41_6_ 0 126 44 Not in stock false false Reshma Shetty annotation1783342 1 identical to E. coli MG1655 between tonB and yciA range1783342 1 4 43 annotation1747359 1 stem loop range1747359 1 11 42 BBa_K1362097 1 pSB4K50 Low copy BioBrick expression backbone carrying Kan resistance 2014-10-07T11:00:00Z 2015-05-08T01:10:04Z false false _1738_ 0 22830 9 Not in stock false false Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha component2409993 1 BBa_G00100 component2409962 1 BBa_G0001 component2409954 1 BBa_G00102 component2409957 1 BBa_G0005 component2409988 1 BBa_I50041 component2410012 1 BBa_K1362463 component2410017 1 BBa_G00000 component2409961 1 BBa_P1007 component2409989 1 BBa_G0006 component2410003 1 BBa_B0042 component2409949 1 BBa_B0042 component2409991 1 BBa_B0053 component2409942 1 BBa_B0044 component2409937 1 BBa_G00001 component2410008 1 BBa_B0043 component2409956 1 BBa_B0062 component2409996 1 BBa_B0055 component2409952 1 BBa_B0054 annotation2409991 1 BBa_B0053 range2409991 1 3203 3274 annotation2409957 1 BBa_G0005 range2409957 1 177 183 annotation2409993 1 BBa_G00100 range2409993 1 3275 3294 annotation2409937 1 BBa_G00001 range2409937 1 1 21 annotation2409962 1 BBa_G0001 range2409962 1 1151 1158 annotation2409956 1 BBa_B0062 range2409956 1 136 176 annotation2409942 1 BBa_B0044 range2409942 1 22 34 annotation2409954 1 BBa_G00102 range2409954 1 116 135 annotation2409988 1 BBa_I50041 range2409988 1 1159 3195 annotation2409952 1 BBa_B0054 range2409952 1 47 115 annotation2410003 1 BBa_B0042 range2410003 1 3373 3384 annotation2409996 1 BBa_B0055 range2409996 1 3295 3372 annotation2409949 1 BBa_B0042 range2409949 1 35 46 annotation2409989 1 BBa_G0006 range2409989 1 3196 3202 annotation2410008 1 BBa_B0043 range2410008 1 3385 3397 annotation2410017 1 BBa_G00000 range2410017 1 3449 3470 annotation2409961 1 BBa_P1007 range2409961 1 184 1150 annotation2410012 1 BBa_K1362463 range2410012 1 3398 3448 BBa_G00102 1 VR Reverse BioBrick primer annealing site (VR binding site) 2006-02-03T12:00:00Z 2015-08-31T04:07:28Z This is the VR sequence to be used in construction of plasmids which include the VR primer site. It is the reverse complement of BBa_G00101. false false _41_6_ 0 126 45 Not in stock false false Reshma Shetty annotation1961229 1 VR range1961229 1 1 20 BBa_B0042 1 TSS Translational stop sequence 2006-02-04T12:00:00Z 2015-08-31T04:07:20Z This part contains a stop codon in all 6 reading frames. Useful for ensuring that translation does not proceed through this part. false false _41_6_ 0 126 45 Not in stock false false Reshma Shetty annotation1797084 1 stop codon (-1 frame) range1797084 1 1 3 annotation1797085 1 stop codon (+3 frame) range1797085 1 6 8 annotation1797083 1 stop codon (+2 frame) range1797083 1 2 4 annotation1797086 1 stop codon (+1 frame) range1797086 1 10 12 annotation1797087 1 stop codon (-2 frame) range1797087 1 9 11 annotation1797088 1 stop codon (-3 frame) range1797088 1 5 7 BBa_B0044 1 TOPO Topoisomerase I mediated cloning site (reverse) 2006-02-04T12:00:00Z 2015-08-31T04:07:20Z Insertion of this site will enable generation of a TOPO vector. The vector will have a T overhang and permit binding of topoisomerase I for direct cloning of PCR products. See http://openwetware.org/wiki/Topoisomerase_I_mediated_TA_cloning for a complete description of Topoisomerase I mediated cloning. This site belongs 3' of the cloning site. It is the reverse complement of BBa_B0043. false false _41_6_ 0 126 45 Not in stock false false Reshma Shetty annotation1797126 1 Nt.BstNB I recognition site range1797126 1 1 5 annotation1797127 1 Arbitrary sequence (could be any bases) range1797127 1 6 8 annotation1797129 1 Generated T overhang range1797129 1 9 9 annotation1797128 1 Topoisomerase I recognition site range1797128 1 9 13 BBa_G00100 1 VF2 Forward primer for sequencing/amplifying BioBrick parts (VF2) 2004-05-24T11:00:00Z 2015-08-31T04:07:28Z Originally named VF2, primer binds upstream of the standard BBa_MCS on biobrick vectors. false false _11_1_ 0 60 7 Not in stock false false Tom Knight annotation1934505 1 VF2 range1934505 1 1 20 BBa_I50041 1 pSC101 pSC101 origin of replication, reverse 2006-03-13T12:00:00Z 2015-08-31T04:07:42Z pSC101 origin of replication which is supposed to have low copy number. This was the origin used in Michael Elowitz's repressilator and in Bujard's pZS4Int-1 vector with mutations introduced to eliminate a SpeI site. It is also the origin of replication in pSB4* series plasmids. This part is the reverse copy of BBa_I50040. false false _41_6_ 0 126 45 Not in stock false false Reshma Shetty annotation2080859 1 difference from canonical pSC101 sequence in Genbank range2080859 1 383 383 annotation2080858 1 difference from canonical pSC101 sequence in Genbank range2080858 1 288 288 annotation1932221 1 inverted repeat range1932221 1 1179 1200 annotation2080856 1 difference from canonical pSC101 sequence in Genbank range2080856 1 36 36 annotation1932229 1 par region range1932229 1 1534 1901 annotation1932228 1 AT rich region range1932228 1 1321 1403 annotation1932218 1 RBS range1932218 1 1154 1159 annotation1932231 1 inverted repeat range1932231 1 1823 1846 annotation1932223 1 rep promoter -35 range1932223 1 1205 1210 annotation1932230 1 inverted repeat range1932230 1 1800 1817 annotation1932234 1 inverted repeat range1932234 1 1886 1899 annotation2080857 1 difference from canonical pSC101 sequence in Genbank range2080857 1 126 126 annotation1932224 1 Mutated SpeI site range1932224 1 1219 1224 annotation1932215 1 putative polyA range1932215 1 76 91 annotation1932222 1 rep promoter -10 range1932222 1 1183 1188 annotation1932233 1 Tn3 integration site range1932233 1 1876 1880 annotation1932227 1 direct repeat range1932227 1 1303 1322 annotation1932217 1 inverted repeat range1932217 1 1154 1176 annotation1932235 1 terminator range1932235 1 1963 1992 annotation1932219 1 RBS range1932219 1 1156 1168 annotation1932220 1 promoter region range1932220 1 1173 1192 annotation1932225 1 direct repeat range1932225 1 1250 1269 annotation1932216 1 repA range1932216 1 202 1152 annotation1932226 1 direct repeat range1932226 1 1282 1301 annotation1932232 1 inverted repeat range1932232 1 1857 1873 BBa_G00000 1 Prefix BioBrick cloning site prefix 2006-03-13T12:00:00Z 2015-08-31T04:07:27Z This part is the standard BioBricks prefix. false true _41_6_ 0 126 45 Not in stock false false Reshma Shetty annotation1934225 1 Prefix range1934225 1 1 22 annotation1934223 1 EcoRI site range1934223 1 1 6 annotation1961228 1 NotI site range1961228 1 7 14 annotation1934224 1 XbaI site range1934224 1 16 21 BBa_G0006 1 scar XbaI/NheI mixed site 2007-02-26T12:00:00Z 2015-08-31T04:07:27Z XbaI and NheI XbaI/NheI mixed site false false _41_ 0 126 70 Not in stock false None. false Reshma Shetty BBa_B0043 1 TOPO Topoisomerase I mediated cloning site (forward) 2006-02-04T12:00:00Z 2015-08-31T04:07:20Z Insertion of this site will enable generation of a TOPO vector. The vector will have a T overhang and permit binding of topoisomerase I for direct cloning of PCR products. See http://openwetware.org/wiki/Topoisomerase_I_mediated_TA_cloning for a complete description of Topoisomerase I mediated cloning. false false _41_6_ 0 126 45 Not in stock false false Reshma Shetty annotation1797102 1 Nt.BstNB I recognition site range1797102 1 9 13 annotation1797104 1 Generated T overhang range1797104 1 5 5 annotation1797101 1 Topoisomerase I recognition site range1797101 1 1 5 annotation1797103 1 Arbitrary sequence (could be any bases) range1797103 1 6 8 BBa_B0062 1 BBa_B0062 Terminator (Reverse B0052) 2004-01-29T12:00:00Z 2015-08-31T04:07:21Z Reverse of B0052, E.coli transcriptional terminator from the ribosomal RNA rrnC operon. false false _1_ 0 24 7 Not in stock false false Caitlin Conboy annotation318637 1 stem_loop range318637 1 14 33 BBa_G0001 1 scar XbaI/SpeI mixed site, reverse 2007-02-26T12:00:00Z 2015-08-31T04:07:27Z XbaI and SpeI XbaI/SpeI mixed site, reverse false false _41_ 0 126 70 Not in stock false None. false Reshma Shetty BBa_B0053 1 BBa_B0053 Terminator (His) 2004-01-29T12:00:00Z 2015-08-31T04:07:20Z Terminator from the E.coli His operon false true _1_ 0 24 7 Not in stock false false Caitlin Conboy annotation318602 1 stem_loop range318602 1 9 43 BBa_K1362463 1 T7/lac pro strong T7 promoter + LacO Regulator 2014-10-07T11:00:00Z 2015-05-08T01:10:06Z GAATTTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCC false false _1738_ 0 22830 9 Not in stock false false Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha annotation2405286 1 scar of cloning into EcoRI site range2405286 1 1 6 annotation2405276 1 lacO reg range2405276 1 24 51 annotation2405261 1 strong T7 promotor range2405261 1 6 24 BBa_G00001 1 Suffix BioBrick cloning site suffix 2006-03-13T12:00:00Z 2015-08-31T04:07:27Z This part is the standard BioBricks suffix. false true _41_6_ 0 126 45 Not in stock false false Reshma Shetty annotation1934227 1 PstI range1934227 1 16 21 annotation1821544 1 Suffix range1821544 1 1 21 annotation1934226 1 SpeI range1934226 1 2 7 annotation1934228 1 NotI range1934228 1 9 16 BBa_G0005 1 scar NheI/SpeI mixed site 2007-02-26T12:00:00Z 2015-08-31T04:07:27Z NheI and SpeI NheI/SpeI mixed site false false _41_ 0 126 70 Not in stock false None. false Reshma Shetty BBa_B0055 1 BBa_B0055 -- No description -- 2005-11-16T12:00:00Z 2015-08-31T04:07:20Z Terminator to be placed upstream of multiple cloning site in new pSB series plasmids. false false _41_6_ 0 126 44 Not in stock false false Reshma Shetty annotation1783301 1 stem loop from bacteriophage T3 range1783301 1 36 57 annotation1783311 1 identical to bacteriophage T3 range1783311 1 28 66 BBa_P1007 1 KanR kanamycin resistance cassette 2006-02-03T12:00:00Z 2015-05-08T01:14:11Z This contains KanR, a terminator and then AmpR and a terminator. false false _41_6_ 0 126 45 Not in stock false false Reshma Shetty annotation1919399 1 kanamycin resistance range1919399 1 1 819 annotation1919401 1 repeat region range1919401 1 924 967 annotation1919400 1 G to C mutation to eliminate XhoI site range1919400 1 787 787 BBa_I50041_sequence 1 gttacattgtcgatctgttcatggtgaacagctttgaatgcaccaaaaactcgtaaaagctctgatgtatctatcttttttacaccgttttcatctgtgcatatggacagttttccctttgatatgtaacggtgaacagttgttctacttttgtttgttagtcttgatgcttcactgatagatacaagagccataagaacctcagatccttccgtatttagccagtatgttctctagtgtggttcgttgtttttgcgtgagccatgagaacgaaccattgagatcatacttactttgcatgtcactcaaaaattttgcctcaaaactggtgagctgaatttttgcagttaaagcatcgtgtagtgtttttcttagtccgttatgtaggtaggaatctgatgtaatggttgttggtattttgtcaccattcatttttatctggttgttctcaagttcggttacgagatccatttgtctatctagttcaacttggaaaatcaacgtatcagtcgggcggcctcgcttatcaaccaccaatttcatattgctgtaagtgtttaaatctttacttattggtttcaaaacccattggttaagccttttaaactcatggtagttattttcaagcattaacatgaacttaaattcatcaaggctaatctctatatttgccttgtgagttttcttttgtgttagttcttttaataaccactcataaatcctcatagagtatttgttttcaaaagacttaacatgttccagattatattttatgaatttttttaactggaaaagataaggcaatatctcttcactaaaaactaattctaatttttcgcttgagaacttggcatagtttgtccactggaaaatctcaaagcctttaaccaaaggattcctgatttccacagttctcgtcatcagctctctggttgctttagctaatacaccataagcattttccctactgatgttcatcatctgagcgtattggttataagtgaacgataccgtccgttctttccttgtagggttttcaatcgtggggttgagtagtgccacacagcataaaattagcttggtttcatgctccgttaagtcatagcgactaatcgctagttcatttgctttgaaaacaactaattcagacatacatctcaattggtctaggtgattttaatcactataccaattgagatgggctagtcaatgataattacatgtccttttcctttgagttgtgggtatctgtaaattctgctagacctttgctggaaaacttgtaaattctgctagaccctctgtaaattccgctagacctttgtgtgttttttttgtttatattcaagtggttataatttatagaataaagaaagaataaaaaaagataaaaagaatagatcccagccctgtgtataactcactactttagtcagttccgcagtattacaaaaggatgtcgcaaacgctgtttgctcctctacaaaacagaccttaaaaccctaaaggcttaagtagcaccctcgcaagctcgggcaaatcgctgaatattccttttgtctccgaccatcaggcacctgagtcgctgtctttttcgtgacattcagttcgctgcgctcacggctctggcagtgaatgggggtaaatggcactacaggcgccttttatggattcatgcaaggaaactacccataatacaagaaaagcccgtcacgggcttctcagggcgttttatggcgggtctgctatgtggtgctatctgactttttgctgttcagcagttcctgccctctgattttccagtctgaccacttcggattatcccgtgacaggtcattcagactggctaatgcacccagtaaggcagcggtatcatcaacaggcttacccgtcttactgtccctagtgcttggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatctatcaacaggagtccaagcgagctcgtaaacttggtctgacag BBa_G0005_sequence 1 gctagta BBa_G0006_sequence 1 ctctagc BBa_B0062_sequence 1 cagataaaaaaaatccttagctttcgctaaggatgatttct BBa_P1007_sequence 1 ttattagaaaaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactcaccgaggcagttccaaagaatggcaaggtcctggtaacggtctgcgattccgacccgtccaacatcaatacaacctattaatttcccctcgtcaaaaataaggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaagagcttgtgcatttctttccagacttgttcaacaggccagccattacgctcgtcatcaaaatcactcgcatcaaccaaaccgttattcatgcgtgattgcgcctgagcaagacgaaatacacgatcgctgttaaaaggacaattacaaacaggaatcgaatgtaaccggcgcaggaacacggccagcgcatcaacaatattttcacctgaatcaggatattcttctaatacctggaaggctgttttcccaggaatcgcggtggtgagtaaccacgcatcatcaggagtacggataaaatgcttgatggtcgggagaggcataaactccgtcagccagttgagacggaccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaacaactctggcgcatcgggcttcccatacaagcgatagattgtcgcacctgattgcccgacattatcgcgagcccatttatacccatataaatcagcgtccatgttggagtttaagcgcggacgggagcaagacgtttcccgttgaatatggctcataacaccccttgtattactgtttatgtaagcagacagttttattgttcatgatgatatatttttatcttgtgcaatgtaacatcagagattttgagacacaacgtggctttgttgaataaatcgaacttttgctgagttgaaggatcag BBa_B0055_sequence 1 aaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaa BBa_B0044_sequence 1 gagtcactaaggg BBa_B0042_sequence 1 ttagttagttag BBa_B0054_sequence 1 attagcagaaagtcaaaagcctccgaccggaggcttttgactaaaacttcccttggggttatcattggg BBa_G00000_sequence 1 gaattcgcggccgcttctagag BBa_G00001_sequence 1 tactagtagcggccgctgcag BBa_B0053_sequence 1 tccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtt BBa_G00100_sequence 1 tgccacctgacgtctaagaa BBa_K1362097_sequence 1 tactagtagcggccgctgcaggagtcactaagggttagttagttagattagcagaaagtcaaaagcctccgaccggaggcttttgactaaaacttcccttggggttatcattggggctcactcaaaggcggtaatcagataaaaaaaatccttagctttcgctaaggatgatttctgctagtattattagaaaaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactcaccgaggcagttccaaagaatggcaaggtcctggtaacggtctgcgattccgacccgtccaacatcaatacaacctattaatttcccctcgtcaaaaataaggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaagagcttgtgcatttctttccagacttgttcaacaggccagccattacgctcgtcatcaaaatcactcgcatcaaccaaaccgttattcatgcgtgattgcgcctgagcaagacgaaatacacgatcgctgttaaaaggacaattacaaacaggaatcgaatgtaaccggcgcaggaacacggccagcgcatcaacaatattttcacctgaatcaggatattcttctaatacctggaaggctgttttcccaggaatcgcggtggtgagtaaccacgcatcatcaggagtacggataaaatgcttgatggtcgggagaggcataaactccgtcagccagttgagacggaccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaacaactctggcgcatcgggcttcccatacaagcgatagattgtcgcacctgattgcccgacattatcgcgagcccatttatacccatataaatcagcgtccatgttggagtttaagcgcggacgggagcaagacgtttcccgttgaatatggctcataacaccccttgtattactgtttatgtaagcagacagttttattgttcatgatgatatatttttatcttgtgcaatgtaacatcagagattttgagacacaacgtggctttgttgaataaatcgaacttttgctgagttgaaggatcagctctagtagttacattgtcgatctgttcatggtgaacagctttgaatgcaccaaaaactcgtaaaagctctgatgtatctatcttttttacaccgttttcatctgtgcatatggacagttttccctttgatatgtaacggtgaacagttgttctacttttgtttgttagtcttgatgcttcactgatagatacaagagccataagaacctcagatccttccgtatttagccagtatgttctctagtgtggttcgttgtttttgcgtgagccatgagaacgaaccattgagatcatacttactttgcatgtcactcaaaaattttgcctcaaaactggtgagctgaatttttgcagttaaagcatcgtgtagtgtttttcttagtccgttatgtaggtaggaatctgatgtaatggttgttggtattttgtcaccattcatttttatctggttgttctcaagttcggttacgagatccatttgtctatctagttcaacttggaaaatcaacgtatcagtcgggcggcctcgcttatcaaccaccaatttcatattgctgtaagtgtttaaatctttacttattggtttcaaaacccattggttaagccttttaaactcatggtagttattttcaagcattaacatgaacttaaattcatcaaggctaatctctatatttgccttgtgagttttcttttgtgttagttcttttaataaccactcataaatcctcatagagtatttgttttcaaaagacttaacatgttccagattatattttatgaatttttttaactggaaaagataaggcaatatctcttcactaaaaactaattctaatttttcgcttgagaacttggcatagtttgtccactggaaaatctcaaagcctttaaccaaaggattcctgatttccacagttctcgtcatcagctctctggttgctttagctaatacaccataagcattttccctactgatgttcatcatctgagcgtattggttataagtgaacgataccgtccgttctttccttgtagggttttcaatcgtggggttgagtagtgccacacagcataaaattagcttggtttcatgctccgttaagtcatagcgactaatcgctagttcatttgctttgaaaacaactaattcagacatacatctcaattggtctaggtgattttaatcactataccaattgagatgggctagtcaatgataattacatgtccttttcctttgagttgtgggtatctgtaaattctgctagacctttgctggaaaacttgtaaattctgctagaccctctgtaaattccgctagacctttgtgtgttttttttgtttatattcaagtggttataatttatagaataaagaaagaataaaaaaagataaaaagaatagatcccagccctgtgtataactcactactttagtcagttccgcagtattacaaaaggatgtcgcaaacgctgtttgctcctctacaaaacagaccttaaaaccctaaaggcttaagtagcaccctcgcaagctcgggcaaatcgctgaatattccttttgtctccgaccatcaggcacctgagtcgctgtctttttcgtgacattcagttcgctgcgctcacggctctggcagtgaatgggggtaaatggcactacaggcgccttttatggattcatgcaaggaaactacccataatacaagaaaagcccgtcacgggcttctcagggcgttttatggcgggtctgctatgtggtgctatctgactttttgctgttcagcagttcctgccctctgattttccagtctgaccacttcggattatcccgtgacaggtcattcagactggctaatgcacccagtaaggcagcggtatcatcaacaggcttacccgtcttactgtccctagtgcttggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatctatcaacaggagtccaagcgagctcgtaaacttggtctgacagctctagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaatttaatacgactcactataggggaattgtgagcggataacaattccccgaattcgcggccgcttctagag BBa_G0001_sequence 1 ctctagta BBa_K1362463_sequence 1 gaatttaatacgactcactataggggaattgtgagcggataacaattcccc BBa_G00102_sequence 1 gctcactcaaaggcggtaat BBa_B0043_sequence 1 cccttagtgactc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z