##gff-version 3
##sequence-region BBa_K1362103 1 1259
BBa_K1362103	.	sequence_feature	30	35	.	+	0	ID=BBa_G0000;Name=BBa_G0000
BBa_K1362103	.	sequence_feature	1258	1259	.	+	0	ID=BBa_K1362465;Name=BBa_K1362465
BBa_K1362103	.	engineered_region	171	1253	.	+	0	ID=BBa_K1362422;Name=BBa_K1362422
BBa_K1362103	.	engineered_region	178	1246	.	+	0	ID=BBa_J04450;Name=BBa_J04450;Parent=BBa_K1362422
BBa_K1362103	.	terminator	1206	1246	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J04450
BBa_K1362103	.	engineered_region	1206	1246	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1362103	.	stop_codon	1239	1239	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1362103	.	polyA_site	1233	1246	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1362103	.	stem_loop	1213	1232	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1362103	.	promoter	178	377	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04450
BBa_K1362103	.	non_covalent_binding_site	266	303	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1362103	.	engineered_region	178	377	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1362103	.	non_covalent_binding_site	343	377	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1362103	.	start_codon	350	350	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1362103	.	promoter	314	319	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1362103	.	CDS	178	265	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1362103	.	promoter	338	343	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1362103	.	CDS	404	1084	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_J04450
BBa_K1362103	.	CDS	404	1078	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1362103	.	engineered_tag	1085	1109	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1362103	.	ribosome_entry_site	386	397	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04450
BBa_K1362103	.	conserved_region	390	393	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1362103	.	terminator	1118	1197	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J04450
BBa_K1362103	.	stem_loop	1129	1172	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1362103	.	engineered_region	1118	1197	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1362103	.	sequence_feature	171	177	.	+	0	ID=BBa_K1362423;Name=BBa_K1362423;Parent=BBa_K1362422
BBa_K1362103	.	sequence_feature	1247	1253	.	+	0	ID=BBa_K1362424;Name=BBa_K1362424;Parent=BBa_K1362422
BBa_K1362103	.	polypeptide_domain	1254	1257	.	+	0	ID=BBa_K1362421;Name=BBa_K1362421
BBa_K1362103	.	stop_codon	1254	1257	.	+	0	ID=annotation2402257;Name=stop;Parent=BBa_K1362421
BBa_K1362103	.	ribosome_entry_site	1	29	.	+	0	ID=BBa_K1362090;Name=BBa_K1362090
BBa_K1362103	.	ribosome_entry_site	11	28	.	+	0	ID=annotation2393795;Name=t7 RBS;Parent=BBa_K1362090
BBa_K1362103	.	sequence_feature	21	28	.	+	0	ID=annotation2393796;Name=Shine-Dalgarno;Parent=BBa_K1362090
BBa_K1362103	.	polypeptide_domain	60	63	.	+	0	ID=BBa_K1362418;Name=BBa_K1362418
BBa_K1362103	.	polypeptide_domain	36	38	.	+	0	ID=BBa_K1362414;Name=BBa_K1362414
BBa_K1362103	.	engineered_region	66	166	.	+	0	ID=BBa_K1362399;Name=BBa_K1362399
BBa_K1362103	.	sequence_alteration	164	164	.	+	0	ID=annotation2411442;Name=to prevent formation of SpeI site;Parent=BBa_K1362399
BBa_K1362103	.	CDS	66	166	.	+	0	ID=annotation2411441;Name=NpuDnaE C-Intein;Parent=BBa_K1362399
BBa_K1362103	.	polypeptide_domain	64	65	.	+	0	ID=BBa_K1362464;Name=BBa_K1362464
BBa_K1362103	.	null	64	65	.	+	0	ID=annotation2405868;Name=2/3Gly;Parent=BBa_K1362464
BBa_K1362103	.	tag	39	59	.	+	0	ID=BBa_K1362431;Name=BBa_K1362431
BBa_K1362103	.	sequence_feature	57	59	.	+	0	ID=annotation2400586;Name=Gly;Parent=BBa_K1362431
BBa_K1362103	.	tag	39	56	.	+	0	ID=annotation2400585;Name=His6;Parent=BBa_K1362431
BBa_K1362103	.	polypeptide_domain	167	170	.	+	0	ID=BBa_K1362420;Name=BBa_K1362420
>BBa_K1362103
aataattttgtttaactttaagaaggagatactagatgcatcatcaccatcaccacggtggtggaatcaaaatagccac
acgtaaatatttaggcaaacaaaatgtctatgacattggagttgagcgcgaccataattttgcactcaaaaatggcttc
atagcagcaggcagagacccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgaca
ggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggcttt
acactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggaga
aatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacgg
tcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaa
ggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccgg
ctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtgg
tgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttc
ccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtg
ctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacat
ggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactac
accatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagat
cgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcg
gtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttataggtctcataataa