##gff-version 3
##sequence-region BBa_K1362104 1 1457
BBa_K1362104	.	ribosome_entry_site	1	29	.	+	0	ID=BBa_K1362090;Name=BBa_K1362090
BBa_K1362104	.	ribosome_entry_site	11	28	.	+	0	ID=annotation2393795;Name=t7 RBS;Parent=BBa_K1362090
BBa_K1362104	.	sequence_feature	21	28	.	+	0	ID=annotation2393796;Name=Shine-Dalgarno;Parent=BBa_K1362090
BBa_K1362104	.	engineered_region	39	1121	.	+	0	ID=BBa_K1362422;Name=BBa_K1362422
BBa_K1362104	.	sequence_feature	1115	1121	.	+	0	ID=BBa_K1362424;Name=BBa_K1362424;Parent=BBa_K1362422
BBa_K1362104	.	engineered_region	46	1114	.	+	0	ID=BBa_J04450;Name=BBa_J04450;Parent=BBa_K1362422
BBa_K1362104	.	ribosome_entry_site	254	265	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04450
BBa_K1362104	.	conserved_region	258	261	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1362104	.	terminator	986	1065	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J04450
BBa_K1362104	.	engineered_region	986	1065	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1362104	.	stem_loop	997	1040	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1362104	.	terminator	1074	1114	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J04450
BBa_K1362104	.	engineered_region	1074	1114	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1362104	.	stop_codon	1107	1107	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1362104	.	polyA_site	1101	1114	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1362104	.	stem_loop	1081	1100	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1362104	.	CDS	272	952	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_J04450
BBa_K1362104	.	CDS	272	946	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1362104	.	engineered_tag	953	977	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1362104	.	promoter	46	245	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04450
BBa_K1362104	.	start_codon	218	218	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1362104	.	CDS	46	133	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1362104	.	non_covalent_binding_site	134	171	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1362104	.	engineered_region	46	245	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1362104	.	promoter	206	211	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1362104	.	non_covalent_binding_site	211	245	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1362104	.	promoter	182	187	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1362104	.	sequence_feature	39	45	.	+	0	ID=BBa_K1362423;Name=BBa_K1362423;Parent=BBa_K1362422
BBa_K1362104	.	polypeptide_domain	36	38	.	+	0	ID=BBa_K1362414;Name=BBa_K1362414
BBa_K1362104	.	polypeptide_domain	1126	1427	.	+	0	ID=BBa_K1362400;Name=BBa_K1362400
BBa_K1362104	.	polypeptide_domain	1122	1125	.	+	0	ID=BBa_K1362415;Name=BBa_K1362415
BBa_K1362104	.	polypeptide_domain	1428	1431	.	+	0	ID=BBa_K1362421;Name=BBa_K1362421
BBa_K1362104	.	stop_codon	1428	1431	.	+	0	ID=annotation2402257;Name=stop;Parent=BBa_K1362421
BBa_K1362104	.	sequence_feature	30	35	.	+	0	ID=BBa_G0000;Name=BBa_G0000
BBa_K1362104	.	tag	1432	1457	.	+	0	ID=BBa_K1362999;Name=BBa_K1362999
BBa_K1362104	.	tag	1434	1451	.	+	0	ID=annotation2400474;Name=iGEMHD;Parent=BBa_K1362999
BBa_K1362104	.	stop_codon	1452	1454	.	+	0	ID=annotation2400473;Name=stop;Parent=BBa_K1362999
BBa_K1362104	.	stop_codon	1455	1457	.	+	0	ID=annotation2400472;Name=stop;Parent=BBa_K1362999
>BBa_K1362104
aataattttgtttaactttaagaaggagatactagatgagagacccaatacgcaaaccgcctctccccgcgcgttggcc
gattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttag
ctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaat
ttcacacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaa
gttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtaccc
agaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacgg
ttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaa
cgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctaca
aagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccac
cgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactac
gacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactgg
acatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgctta
ataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactggg
cctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctg
cgtttataggtctcatgcttaagctatgaaacggaaatattgacagtagaatatggattattaccgattggtaaaattg
tagaaaagcgcatcgaatgtactgtttatagcgttgataataatggaaatatttatacacaacctgtagcacaatggca
cgatcgcggagaacaagaggtgtttgagtattgtttggaagatggttcattgattcgggcaacaaaagaccataagttt
atgactgttgatggtcaaatgttgccaattgatgaaatatttgaacgtgaattggatttgatgcgggttgataatttgc
cgaattaataaatcggtgaaatgcacgactgatag