LOCUS BBa_K1362106 2523 bp DNA linear 22-Nov-2024 DEFINITION RBS + NpuDnaE N-intein RFC[105] assembly construct with additional C-teminal insertion site ACCESSION BBa_K1362106 VERSION BBa_K1362106.1 FEATURES Location/Qualifiers RBS 11..28 /label=t7 RBS misc_feature 21..28 /label=Shine-Dalgarno RBS 1..29 /label=BBa_K1362090 misc_feature 30..35 /label=BBa_G0000 misc_feature 36..38 /label=BBa_K1362414 misc_feature 39..45 /label=BBa_K1362423 CDS 46..133 /label=end of LacI coding region (inactive) misc_feature 46..245 /label=BBa_R0010 misc_feature 134..171 /label=CAP binding site promoter 182..187 /label=-35 promoter 206..211 /label=-10 misc_feature 211..245 /label=LacI binding site misc_feature 218..218 /label=start promoter 46..245 /label=BBa_R0010 misc_feature 258..261 /label=conserved RBS 254..265 /label=BBa_B0034 CDS 272..946 /label=mrfp1 misc_feature 953..977 /label=Help:Barcodes CDS 272..952 /label=BBa_E1010 stem_loop 997..1040 /label=stem_loop misc_feature 986..1065 /label=BBa_B0010 terminator 986..1065 /label=BBa_B0010 stem_loop 1081..1100 /label=T7 TE misc_feature 1107..1107 /label=stop polyA_site 1101..1114 /label=polya misc_feature 1074..1114 /label=BBa_B0012 terminator 1074..1114 /label=BBa_B0012 misc_feature 46..1114 /label=BBa_J04450 misc_feature 1115..1121 /label=BBa_K1362424 misc_feature 39..1121 /label=BBa_K1362422 misc_feature 1122..1125 /label=BBa_K1362415 misc_feature 1126..1427 /label=BBa_K1362400 misc_feature 1430..1433 /label=RFC[???] C misc_feature 1428..1433 /label=GlyGly misc_feature 1428..1433 /label=BBa_K1362417 misc_feature 1434..1440 /label=BBa_K1362423 CDS 1441..1528 /label=end of LacI coding region (inactive) misc_feature 1441..1640 /label=BBa_R0010 misc_feature 1529..1566 /label=CAP binding site promoter 1577..1582 /label=-35 promoter 1601..1606 /label=-10 misc_feature 1606..1640 /label=LacI binding site misc_feature 1613..1613 /label=start promoter 1441..1640 /label=BBa_R0010 misc_feature 1653..1656 /label=conserved RBS 1649..1660 /label=BBa_B0034 CDS 1667..2341 /label=mrfp1 misc_feature 2348..2372 /label=Help:Barcodes CDS 1667..2347 /label=BBa_E1010 stem_loop 2392..2435 /label=stem_loop misc_feature 2381..2460 /label=BBa_B0010 terminator 2381..2460 /label=BBa_B0010 stem_loop 2476..2495 /label=T7 TE misc_feature 2502..2502 /label=stop polyA_site 2496..2509 /label=polya misc_feature 2469..2509 /label=BBa_B0012 terminator 2469..2509 /label=BBa_B0012 misc_feature 1441..2509 /label=BBa_J04450 misc_feature 2510..2516 /label=BBa_K1362424 misc_feature 1434..2516 /label=BBa_K1362422 misc_feature 2517..2520 /label=stop misc_feature 2517..2520 /label=BBa_K1362421 misc_feature 2521..2522 /label=stop misc_feature 2521..2523 /label=BBa_K1362437 ORIGIN 1 aataattttg tttaacttta agaaggagat actagatgag agacccaata cgcaaaccgc 61 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga 121 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg 181 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc 241 acacatacta gagaaagagg agaaatacta gatggcttcc tccgaagacg ttatcaaaga 301 gttcatgcgt ttcaaagttc gtatggaagg ttccgttaac ggtcacgagt tcgaaatcga 361 aggtgaaggt gaaggtcgtc cgtacgaagg tacccagacc gctaaactga aagttaccaa 421 aggtggtccg ctgccgttcg cttgggacat cctgtccccg cagttccagt acggttccaa 481 agcttacgtt aaacacccgg ctgacatccc ggactacctg aaactgtcct tcccggaagg 541 tttcaaatgg gaacgtgtta tgaacttcga agacggtggt gttgttaccg ttacccagga 601 ctcctccctg caagacggtg agttcatcta caaagttaaa ctgcgtggta ccaacttccc 661 gtccgacggt ccggttatgc agaaaaaaac catgggttgg gaagcttcca ccgaacgtat 721 gtacccggaa gacggtgctc tgaaaggtga aatcaaaatg cgtctgaaac tgaaagacgg 781 tggtcactac gacgctgaag ttaaaaccac ctacatggct aaaaaaccgg ttcagctgcc 841 gggtgcttac aaaaccgaca tcaaactgga catcacctcc cacaacgaag actacaccat 901 cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaat aacgctgata 961 gtgctagtgt agatcgctac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa 1021 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact 1081 ggctcacctt cgggtgggcc tttctgcgtt tataggtctc atgcttaagc tatgaaacgg 1141 aaatattgac agtagaatat ggattattac cgattggtaa aattgtagaa aagcgcatcg 1201 aatgtactgt ttatagcgtt gataataatg gaaatattta tacacaacct gtagcacaat 1261 ggcacgatcg cggagaacaa gaggtgtttg agtattgttt ggaagatggt tcattgattc 1321 gggcaacaaa agaccataag tttatgactg ttgatggtca aatgttgcca attgatgaaa 1381 tatttgaacg tgaattggat ttgatgcggg ttgataattt gccgaatggt ggtagagacc 1441 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 1501 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 1561 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 1621 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg cttcctccga 1681 agacgttatc aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca 1741 cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa 1801 actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt 1861 ccagtacggt tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact 1921 gtccttcccg gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt 1981 taccgttacc caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg 2041 tggtaccaac ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc 2101 ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct 2161 gaaactgaaa gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa 2221 accggttcag ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa 2281 cgaagactac accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc 2341 ttaataacgc tgatagtgct agtgtagatc gctactagag ccaggcatca aataaaacga 2401 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc 2461 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatag gtctcataat 2521 aaa //