LOCUS BBa_K1362108 2731 bp DNA linear 23-Dec-2024 DEFINITION RBS + NpuDnaE N&C-intein coexpression RFC[105] assembly construct (with His6) ACCESSION BBa_K1362108 VERSION BBa_K1362108.1 FEATURES Location/Qualifiers RBS 11..28 /label=t7 RBS misc_feature 21..28 /label=Shine-Dalgarno RBS 1..29 /label=BBa_K1362090 misc_feature 30..35 /label=BBa_G0000 misc_feature 36..38 /label=BBa_K1362414 misc_feature 39..45 /label=BBa_K1362423 CDS 46..133 /label=end of LacI coding region (inactive) misc_feature 46..245 /label=BBa_R0010 misc_feature 134..171 /label=CAP binding site promoter 182..187 /label=-35 promoter 206..211 /label=-10 misc_feature 211..245 /label=LacI binding site misc_feature 218..218 /label=start promoter 46..245 /label=BBa_R0010 misc_feature 258..261 /label=conserved RBS 254..265 /label=BBa_B0034 CDS 272..946 /label=mrfp1 misc_feature 953..977 /label=Help:Barcodes CDS 272..952 /label=BBa_E1010 stem_loop 997..1040 /label=stem_loop misc_feature 986..1065 /label=BBa_B0010 terminator 986..1065 /label=BBa_B0010 stem_loop 1081..1100 /label=T7 TE misc_feature 1107..1107 /label=stop polyA_site 1101..1114 /label=polya misc_feature 1074..1114 /label=BBa_B0012 terminator 1074..1114 /label=BBa_B0012 misc_feature 46..1114 /label=BBa_J04450 misc_feature 1115..1121 /label=BBa_K1362424 misc_feature 39..1121 /label=BBa_K1362422 misc_feature 1122..1125 /label=BBa_K1362415 misc_feature 1126..1427 /label=BBa_K1362400 misc_feature 1430..1433 /label=RFC[???] C misc_feature 1428..1433 /label=GlyGly misc_feature 1428..1433 /label=BBa_K1362417 misc_feature 1437..1454 /label=His6 misc_feature 1455..1457 /label=Stop misc_feature 1434..1457 /label=BBa_K1362430 misc_feature 1458..1461 /label=stop misc_feature 1458..1461 /label=BBa_K1362421 misc_feature 1462..1463 /label=stop misc_feature 1462..1464 /label=BBa_K1362437 misc_feature 1..1464 /label=BBa_K1362100 RBS 1483..1500 /label=t7 RBS misc_feature 1493..1500 /label=Shine-Dalgarno RBS 1473..1501 /label=BBa_K1362090 misc_feature 1502..1507 /label=BBa_G0000 misc_feature 1508..1510 /label=BBa_K1362414 misc_feature 1511..1528 /label=His6 misc_feature 1529..1531 /label=Gly misc_feature 1511..1531 /label=BBa_K1362431 misc_feature 1532..1535 /label=BBa_K1362418 misc_feature 1536..1537 /label=2/3Gly misc_feature 1536..1537 /label=BBa_K1362464 misc_feature 1538..1638 /label=BBa_K1362401 misc_feature 1639..1642 /label=BBa_K1362419 misc_feature 1643..1649 /label=BBa_K1362423 CDS 1650..1737 /label=end of LacI coding region (inactive) misc_feature 1650..1849 /label=BBa_R0010 misc_feature 1738..1775 /label=CAP binding site promoter 1786..1791 /label=-35 promoter 1810..1815 /label=-10 misc_feature 1815..1849 /label=LacI binding site misc_feature 1822..1822 /label=start promoter 1650..1849 /label=BBa_R0010 misc_feature 1862..1865 /label=conserved RBS 1858..1869 /label=BBa_B0034 CDS 1876..2550 /label=mrfp1 misc_feature 2557..2581 /label=Help:Barcodes CDS 1876..2556 /label=BBa_E1010 stem_loop 2601..2644 /label=stem_loop misc_feature 2590..2669 /label=BBa_B0010 terminator 2590..2669 /label=BBa_B0010 stem_loop 2685..2704 /label=T7 TE misc_feature 2711..2711 /label=stop polyA_site 2705..2718 /label=polya misc_feature 2678..2718 /label=BBa_B0012 terminator 2678..2718 /label=BBa_B0012 misc_feature 1650..2718 /label=BBa_J04450 misc_feature 2719..2725 /label=BBa_K1362424 misc_feature 1643..2725 /label=BBa_K1362422 misc_feature 2726..2729 /label=stop misc_feature 2726..2729 /label=BBa_K1362421 misc_feature 2730..2731 /label=BBa_K1362465 misc_feature 1473..2731 /label=BBa_K1362101 ORIGIN 1 aataattttg tttaacttta agaaggagat actagatgag agacccaata cgcaaaccgc 61 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga 121 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg 181 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc 241 acacatacta gagaaagagg agaaatacta gatggcttcc tccgaagacg ttatcaaaga 301 gttcatgcgt ttcaaagttc gtatggaagg ttccgttaac ggtcacgagt tcgaaatcga 361 aggtgaaggt gaaggtcgtc cgtacgaagg tacccagacc gctaaactga aagttaccaa 421 aggtggtccg ctgccgttcg cttgggacat cctgtccccg cagttccagt acggttccaa 481 agcttacgtt aaacacccgg ctgacatccc ggactacctg aaactgtcct tcccggaagg 541 tttcaaatgg gaacgtgtta tgaacttcga agacggtggt gttgttaccg ttacccagga 601 ctcctccctg caagacggtg agttcatcta caaagttaaa ctgcgtggta ccaacttccc 661 gtccgacggt ccggttatgc agaaaaaaac catgggttgg gaagcttcca ccgaacgtat 721 gtacccggaa gacggtgctc tgaaaggtga aatcaaaatg cgtctgaaac tgaaagacgg 781 tggtcactac gacgctgaag ttaaaaccac ctacatggct aaaaaaccgg ttcagctgcc 841 gggtgcttac aaaaccgaca tcaaactgga catcacctcc cacaacgaag actacaccat 901 cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaat aacgctgata 961 gtgctagtgt agatcgctac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa 1021 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact 1081 ggctcacctt cgggtgggcc tttctgcgtt tataggtctc atgcttaagc tatgaaacgg 1141 aaatattgac agtagaatat ggattattac cgattggtaa aattgtagaa aagcgcatcg 1201 aatgtactgt ttatagcgtt gataataatg gaaatattta tacacaacct gtagcacaat 1261 ggcacgatcg cggagaacaa gaggtgtttg agtattgttt ggaagatggt tcattgattc 1321 gggcaacaaa agaccataag tttatgactg ttgatggtca aatgttgcca attgatgaaa 1381 tatttgaacg tgaattggat ttgatgcggg ttgataattt gccgaatggt ggtggtcatc 1441 atcaccatca ccattagtaa taaatactag agaataattt tgtttaactt taagaaggag 1501 atactagatg catcatcacc atcaccacgg tggtggaatc aaaatagcca cacgtaaata 1561 tttaggcaaa caaaatgtct atgacattgg agttgagcgc gaccataatt ttgcactcaa 1621 aaatggcttc atagcttcca acagagaccc aatacgcaaa ccgcctctcc ccgcgcgttg 1681 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg 1741 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct 1801 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacat actagagaaa 1861 gaggagaaat actagatggc ttcctccgaa gacgttatca aagagttcat gcgtttcaaa 1921 gttcgtatgg aaggttccgt taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt 1981 cgtccgtacg aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg 2041 ttcgcttggg acatcctgtc cccgcagttc cagtacggtt ccaaagctta cgttaaacac 2101 ccggctgaca tcccggacta cctgaaactg tccttcccgg aaggtttcaa atgggaacgt 2161 gttatgaact tcgaagacgg tggtgttgtt accgttaccc aggactcctc cctgcaagac 2221 ggtgagttca tctacaaagt taaactgcgt ggtaccaact tcccgtccga cggtccggtt 2281 atgcagaaaa aaaccatggg ttgggaagct tccaccgaac gtatgtaccc ggaagacggt 2341 gctctgaaag gtgaaatcaa aatgcgtctg aaactgaaag acggtggtca ctacgacgct 2401 gaagttaaaa ccacctacat ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc 2461 gacatcaaac tggacatcac ctcccacaac gaagactaca ccatcgttga acagtacgaa 2521 cgtgctgaag gtcgtcactc caccggtgct taataacgct gatagtgcta gtgtagatcg 2581 ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt 2641 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg 2701 ggcctttctg cgtttatagg tctcataata a //