##gff-version 3
##sequence-region BBa_K1362109 1 2731
BBa_K1362109	.	engineered_region	1	1464	.	+	0	ID=BBa_K1362102;Name=BBa_K1362102
BBa_K1362109	.	sequence_feature	30	35	.	+	0	ID=BBa_G0000;Name=BBa_G0000;Parent=BBa_K1362102
BBa_K1362109	.	engineered_region	39	1121	.	+	0	ID=BBa_K1362422;Name=BBa_K1362422;Parent=BBa_K1362102
BBa_K1362109	.	sequence_feature	1115	1121	.	+	0	ID=BBa_K1362424;Name=BBa_K1362424;Parent=BBa_K1362422
BBa_K1362109	.	engineered_region	46	1114	.	+	0	ID=BBa_J04450;Name=BBa_J04450;Parent=BBa_K1362422
BBa_K1362109	.	CDS	272	952	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_J04450
BBa_K1362109	.	CDS	272	946	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1362109	.	engineered_tag	953	977	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1362109	.	promoter	46	245	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04450
BBa_K1362109	.	start_codon	218	218	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1362109	.	non_covalent_binding_site	134	171	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1362109	.	engineered_region	46	245	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1362109	.	promoter	206	211	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1362109	.	non_covalent_binding_site	211	245	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1362109	.	promoter	182	187	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1362109	.	CDS	46	133	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1362109	.	ribosome_entry_site	254	265	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04450
BBa_K1362109	.	conserved_region	258	261	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1362109	.	terminator	986	1065	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J04450
BBa_K1362109	.	stem_loop	997	1040	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1362109	.	engineered_region	986	1065	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1362109	.	terminator	1074	1114	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J04450
BBa_K1362109	.	polyA_site	1101	1114	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1362109	.	stem_loop	1081	1100	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1362109	.	engineered_region	1074	1114	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1362109	.	stop_codon	1107	1107	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1362109	.	sequence_feature	39	45	.	+	0	ID=BBa_K1362423;Name=BBa_K1362423;Parent=BBa_K1362422
BBa_K1362109	.	polypeptide_domain	1126	1427	.	+	0	ID=BBa_K1362400;Name=BBa_K1362400;Parent=BBa_K1362102
BBa_K1362109	.	tag	1434	1457	.	+	0	ID=BBa_K1362430;Name=BBa_K1362430;Parent=BBa_K1362102
BBa_K1362109	.	stop_codon	1455	1457	.	+	0	ID=annotation2394833;Name=Stop;Parent=BBa_K1362430
BBa_K1362109	.	tag	1437	1454	.	+	0	ID=annotation2394832;Name=His6;Parent=BBa_K1362430
BBa_K1362109	.	sequence_feature	1462	1464	.	+	0	ID=BBa_K1362437;Name=BBa_K1362437;Parent=BBa_K1362102
BBa_K1362109	.	stop_codon	1462	1463	.	+	0	ID=annotation2402256;Name=stop;Parent=BBa_K1362437
BBa_K1362109	.	polypeptide_domain	36	38	.	+	0	ID=BBa_K1362414;Name=BBa_K1362414;Parent=BBa_K1362102
BBa_K1362109	.	polypeptide_domain	1458	1461	.	+	0	ID=BBa_K1362421;Name=BBa_K1362421;Parent=BBa_K1362102
BBa_K1362109	.	stop_codon	1458	1461	.	+	0	ID=annotation2402257;Name=stop;Parent=BBa_K1362421
BBa_K1362109	.	polypeptide_domain	1122	1125	.	+	0	ID=BBa_K1362416;Name=BBa_K1362416;Parent=BBa_K1362102
BBa_K1362109	.	polypeptide_domain	1428	1433	.	+	0	ID=BBa_K1362417;Name=BBa_K1362417;Parent=BBa_K1362102
BBa_K1362109	.	conserved_region	1430	1433	.	+	0	ID=annotation2405925;Name=RFC[???] C;Parent=BBa_K1362417
BBa_K1362109	.	null	1428	1433	.	+	0	ID=annotation2405926;Name=GlyGly;Parent=BBa_K1362417
BBa_K1362109	.	ribosome_entry_site	1	29	.	+	0	ID=BBa_K1362090;Name=BBa_K1362090;Parent=BBa_K1362102
BBa_K1362109	.	sequence_feature	21	28	.	+	0	ID=annotation2393796;Name=Shine-Dalgarno;Parent=BBa_K1362090
BBa_K1362109	.	ribosome_entry_site	11	28	.	+	0	ID=annotation2393795;Name=t7 RBS;Parent=BBa_K1362090
BBa_K1362109	.	engineered_region	1473	2731	.	+	0	ID=BBa_K1362103;Name=BBa_K1362103
BBa_K1362109	.	sequence_feature	1502	1507	.	+	0	ID=BBa_G0000;Name=BBa_G0000;Parent=BBa_K1362103
BBa_K1362109	.	tag	1511	1531	.	+	0	ID=BBa_K1362431;Name=BBa_K1362431;Parent=BBa_K1362103
BBa_K1362109	.	tag	1511	1528	.	+	0	ID=annotation2400585;Name=His6;Parent=BBa_K1362431
BBa_K1362109	.	sequence_feature	1529	1531	.	+	0	ID=annotation2400586;Name=Gly;Parent=BBa_K1362431
BBa_K1362109	.	polypeptide_domain	1639	1642	.	+	0	ID=BBa_K1362420;Name=BBa_K1362420;Parent=BBa_K1362103
BBa_K1362109	.	polypeptide_domain	1536	1537	.	+	0	ID=BBa_K1362464;Name=BBa_K1362464;Parent=BBa_K1362103
BBa_K1362109	.	null	1536	1537	.	+	0	ID=annotation2405868;Name=2/3Gly;Parent=BBa_K1362464
BBa_K1362109	.	polypeptide_domain	1532	1535	.	+	0	ID=BBa_K1362418;Name=BBa_K1362418;Parent=BBa_K1362103
BBa_K1362109	.	polypeptide_domain	1508	1510	.	+	0	ID=BBa_K1362414;Name=BBa_K1362414;Parent=BBa_K1362103
BBa_K1362109	.	polypeptide_domain	2726	2729	.	+	0	ID=BBa_K1362421;Name=BBa_K1362421;Parent=BBa_K1362103
BBa_K1362109	.	stop_codon	2726	2729	.	+	0	ID=annotation2402257;Name=stop;Parent=BBa_K1362421
BBa_K1362109	.	engineered_region	1538	1638	.	+	0	ID=BBa_K1362399;Name=BBa_K1362399;Parent=BBa_K1362103
BBa_K1362109	.	sequence_alteration	1636	1636	.	+	0	ID=annotation2411442;Name=to prevent formation of SpeI site;Parent=BBa_K1362399
BBa_K1362109	.	CDS	1538	1638	.	+	0	ID=annotation2411441;Name=NpuDnaE C-Intein;Parent=BBa_K1362399
BBa_K1362109	.	sequence_feature	2730	2731	.	+	0	ID=BBa_K1362465;Name=BBa_K1362465;Parent=BBa_K1362103
BBa_K1362109	.	ribosome_entry_site	1473	1501	.	+	0	ID=BBa_K1362090;Name=BBa_K1362090;Parent=BBa_K1362103
BBa_K1362109	.	sequence_feature	1493	1500	.	+	0	ID=annotation2393796;Name=Shine-Dalgarno;Parent=BBa_K1362090
BBa_K1362109	.	ribosome_entry_site	1483	1500	.	+	0	ID=annotation2393795;Name=t7 RBS;Parent=BBa_K1362090
BBa_K1362109	.	engineered_region	1643	2725	.	+	0	ID=BBa_K1362422;Name=BBa_K1362422;Parent=BBa_K1362103
BBa_K1362109	.	sequence_feature	2719	2725	.	+	0	ID=BBa_K1362424;Name=BBa_K1362424;Parent=BBa_K1362422
BBa_K1362109	.	engineered_region	1650	2718	.	+	0	ID=BBa_J04450;Name=BBa_J04450;Parent=BBa_K1362422
BBa_K1362109	.	CDS	1876	2556	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_J04450
BBa_K1362109	.	CDS	1876	2550	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1362109	.	engineered_tag	2557	2581	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1362109	.	promoter	1650	1849	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04450
BBa_K1362109	.	start_codon	1822	1822	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1362109	.	non_covalent_binding_site	1738	1775	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1362109	.	engineered_region	1650	1849	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1362109	.	promoter	1810	1815	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1362109	.	non_covalent_binding_site	1815	1849	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1362109	.	promoter	1786	1791	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1362109	.	CDS	1650	1737	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1362109	.	ribosome_entry_site	1858	1869	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04450
BBa_K1362109	.	conserved_region	1862	1865	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1362109	.	terminator	2590	2669	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J04450
BBa_K1362109	.	stem_loop	2601	2644	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1362109	.	engineered_region	2590	2669	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1362109	.	terminator	2678	2718	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J04450
BBa_K1362109	.	polyA_site	2705	2718	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1362109	.	stem_loop	2685	2704	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1362109	.	engineered_region	2678	2718	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1362109	.	stop_codon	2711	2711	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1362109	.	sequence_feature	1643	1649	.	+	0	ID=BBa_K1362423;Name=BBa_K1362423;Parent=BBa_K1362422
>BBa_K1362109
aataattttgtttaactttaagaaggagatactagatgagagacccaatacgcaaaccgcctctccccgcgcgttggcc
gattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttag
ctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaat
ttcacacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaa
gttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtaccc
agaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacgg
ttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaa
cgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctaca
aagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccac
cgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactac
gacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactgg
acatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgctta
ataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactggg
cctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctg
cgtttataggtctcaggcttaagctatgaaacggaaatattgacagtagaatatggattattaccgattggtaaaattg
tagaaaagcgcatcgaatgtactgtttatagcgttgataataatggaaatatttatacacaacctgtagcacaatggca
cgatcgcggagaacaagaggtgtttgagtattgtttggaagatggttcattgattcgggcaacaaaagaccataagttt
atgactgttgatggtcaaatgttgccaattgatgaaatatttgaacgtgaattggatttgatgcgggttgataatttgc
cgaatggtggtggtcatcatcaccatcaccattagtaataaatactagagaataattttgtttaactttaagaaggaga
tactagatgcatcatcaccatcaccacggtggtggaatcaaaatagccacacgtaaatatttaggcaaacaaaatgtct
atgacattggagttgagcgcgaccataattttgcactcaaaaatggcttcatagcagcaggcagagacccaatacgcaa
accgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgag
cgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgt
gtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcctccgaagacgtt
atcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtg
aaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacat
cctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtcc
ttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccc
tgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaa
aaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctg
aaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtg
cttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctga
aggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaac
gaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactgg
ctcaccttcgggtgggcctttctgcgtttataggtctcataataa