LOCUS       BBa_K1362121            1172 bp    DNA     linear       25-Nov-2024
DEFINITION  RBS + SspGyrB C-intein RFC[105] assembly construct (with His6)
ACCESSION   BBa_K1362121
VERSION     BBa_K1362121.1  
FEATURES             Location/Qualifiers
     RBS             11..28
                     /label=t7 RBS
     misc_feature    21..28
                     /label=Shine-Dalgarno
     RBS             1..29
                     /label=BBa_K1362090
     misc_feature    30..35
                     /label=BBa_G0000
     misc_feature    36..38
                     /label=BBa_K1362414
     misc_feature    39..56
                     /label=His6
     misc_feature    57..59
                     /label=Gly
     misc_feature    39..59
                     /label=BBa_K1362431
     misc_feature    60..63
                     /label=BBa_K1362418
     misc_feature    64..65
                     /label=BBa_K1362467
     misc_feature    66..79
                     /label=BBa_K1362407
     misc_feature    80..83
                     /label=BBa_K1362419
     misc_feature    84..90
                     /label=BBa_K1362423
     CDS             91..178
                     /label=end of LacI coding region (inactive)
     misc_feature    91..290
                     /label=BBa_R0010
     misc_feature    179..216
                     /label=CAP binding site
     promoter        227..232
                     /label=-35
     promoter        251..256
                     /label=-10
     misc_feature    256..290
                     /label=LacI binding site
     misc_feature    263..263
                     /label=start
     promoter        91..290
                     /label=BBa_R0010
     misc_feature    303..306
                     /label=conserved
     RBS             299..310
                     /label=BBa_B0034
     CDS             317..991
                     /label=mrfp1
     misc_feature    998..1022
                     /label=Help:Barcodes
     CDS             317..997
                     /label=BBa_E1010
     stem_loop       1042..1085
                     /label=stem_loop
     misc_feature    1031..1110
                     /label=BBa_B0010
     terminator      1031..1110
                     /label=BBa_B0010
     stem_loop       1126..1145
                     /label=T7 TE
     misc_feature    1152..1152
                     /label=stop
     polyA_site      1146..1159
                     /label=polya
     misc_feature    1119..1159
                     /label=BBa_B0012
     terminator      1119..1159
                     /label=BBa_B0012
     misc_feature    91..1159
                     /label=BBa_J04450
     misc_feature    1160..1166
                     /label=BBa_K1362424
     misc_feature    84..1166
                     /label=BBa_K1362422
     misc_feature    1167..1170
                     /label=stop
     misc_feature    1167..1170
                     /label=BBa_K1362421
     misc_feature    1171..1172
                     /label=BBa_K1362465
ORIGIN
        1 aataattttg tttaacttta agaaggagat actagatgca tcatcaccat caccacggtg
       61 gtggtggtgt tttcgttcac aacagagacc caatacgcaa accgcctctc cccgcgcgtt
      121 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
      181 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
      241 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca tactagagaa
      301 agaggagaaa tactagatgg cttcctccga agacgttatc aaagagttca tgcgtttcaa
      361 agttcgtatg gaaggttccg ttaacggtca cgagttcgaa atcgaaggtg aaggtgaagg
      421 tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt accaaaggtg gtccgctgcc
      481 gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt tccaaagctt acgttaaaca
      541 cccggctgac atcccggact acctgaaact gtccttcccg gaaggtttca aatgggaacg
      601 tgttatgaac ttcgaagacg gtggtgttgt taccgttacc caggactcct ccctgcaaga
      661 cggtgagttc atctacaaag ttaaactgcg tggtaccaac ttcccgtccg acggtccggt
      721 tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc cggaagacgg
      781 tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc actacgacgc
      841 tgaagttaaa accacctaca tggctaaaaa accggttcag ctgccgggtg cttacaaaac
      901 cgacatcaaa ctggacatca cctcccacaa cgaagactac accatcgttg aacagtacga
      961 acgtgctgaa ggtcgtcact ccaccggtgc ttaataacgc tgatagtgct agtgtagatc
     1021 gctactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     1081 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
     1141 gggcctttct gcgtttatag gtctcataat aa
//