TerDnaE3(N
type: ComponentDefinition
persistent identity: BBa K1362130
display id: BBa_K1362130
version: 1
was generated by: iGEM to SBOL conversion
description: RBS + TerDnaE3 N-intein RFC[105] assembly construct (with His6)
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:10:04Z
mutable provenance: ???
owned by: james
owned by: myers
mutable description: ???
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 12377
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: ???
star: false
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K1362421
component: BBa_K1362430
component: BBa_K1362090
component: BBa_K1362437
component: BBa_K1362414
component: BBa_G0000
component: BBa_K1362415
component: BBa_K1362422
component: BBa_K1362417
component: BBa_K1362404
sequence annotation: BBa_K1362090
sequence annotation: BBa_G0000
sequence annotation: BBa_K1362414
sequence annotation: BBa_K1362422
sequence annotation: BBa_K1362415
sequence annotation: BBa_K1362404
sequence annotation: BBa_K1362417
sequence annotation: BBa_K1362430
sequence annotation: BBa_K1362421
sequence annotation: BBa_K1362437
sequence: https://synbiohub.org/public/igem/BBa_K1362130_sequence/1

BBa_K1362421
type: Component
persistent identity: component2410504
display id: component2410504
version: 1
top level: TerDnaE3(N
definition: RFC105 Z
access: public

BBa_K1362430
type: Component
persistent identity: component2410502
display id: component2410502
version: 1
top level: TerDnaE3(N
definition: G-His6(N)
access: public

BBa_K1362090
type: Component
persistent identity: component2410471
display id: component2410471
version: 1
top level: TerDnaE3(N
definition: T7 RBS
access: public

BBa_K1362437
type: Component
persistent identity: component2410506
display id: component2410506
version: 1
top level: TerDnaE3(N
definition: Stop
access: public

BBa_K1362414
type: Component
persistent identity: component2410473
display id: component2410473
version: 1
top level: TerDnaE3(N
definition: RFC105 A
access: public

BBa_G0000
type: Component
persistent identity: component2410472
display id: component2410472
version: 1
top level: TerDnaE3(N
definition: scar
access: public

BBa_K1362415
type: Component
persistent identity: component2410495
display id: component2410495
version: 1
top level: TerDnaE3(N
definition: RFC105 NN
access: public

BBa_K1362422
type: Component
persistent identity: component2410494
display id: component2410494
version: 1
top level: TerDnaE3(N
definition: mRFP sel.
access: public

BBa_K1362417
type: Component
persistent identity: component2410499
display id: component2410499
version: 1
top level: TerDnaE3(N
definition: RFC105 NC
access: public

BBa_K1362404
type: Component
persistent identity: component2410496
display id: component2410496
version: 1
top level: TerDnaE3(N
definition: TerDnaE3(N
access: public

BBa_K1362090
type: SequenceAnnotation
persistent identity: annotation2410471
display id: annotation2410471
version: 1
top level: TerDnaE3(N
location: https://synbiohub.org/public/igem/BBa_K1362130/annotation2410471/range2410471/1
component: BBa_K1362090

https://synbiohub.org/public/igem/BBa_K1362130/annotation2410471/range2410471/1
type: Range
persistent identity: range2410471
display id: range2410471
version: 1
top level: TerDnaE3(N
direction: direction/reverse
start: 1
end: 29
orientation: inline

BBa_G0000
type: SequenceAnnotation
persistent identity: annotation2410472
display id: annotation2410472
version: 1
top level: TerDnaE3(N
location: https://synbiohub.org/public/igem/BBa_K1362130/annotation2410472/range2410472/1
component: BBa_G0000

https://synbiohub.org/public/igem/BBa_K1362130/annotation2410472/range2410472/1
type: Range
persistent identity: range2410472
display id: range2410472
version: 1
top level: TerDnaE3(N
direction: direction/reverse
start: 30
end: 35
orientation: inline

BBa_K1362414
type: SequenceAnnotation
persistent identity: annotation2410473
display id: annotation2410473
version: 1
top level: TerDnaE3(N
location: https://synbiohub.org/public/igem/BBa_K1362130/annotation2410473/range2410473/1
component: BBa_K1362414

https://synbiohub.org/public/igem/BBa_K1362130/annotation2410473/range2410473/1
type: Range
persistent identity: range2410473
display id: range2410473
version: 1
top level: TerDnaE3(N
direction: direction/reverse
start: 36
end: 38
orientation: inline

BBa_K1362422
type: SequenceAnnotation
persistent identity: annotation2410494
display id: annotation2410494
version: 1
top level: TerDnaE3(N
location: https://synbiohub.org/public/igem/BBa_K1362130/annotation2410494/range2410494/1
component: BBa_K1362422

https://synbiohub.org/public/igem/BBa_K1362130/annotation2410494/range2410494/1
type: Range
persistent identity: range2410494
display id: range2410494
version: 1
top level: TerDnaE3(N
direction: direction/reverse
start: 39
end: 1121
orientation: inline

BBa_K1362415
type: SequenceAnnotation
persistent identity: annotation2410495
display id: annotation2410495
version: 1
top level: TerDnaE3(N
location: https://synbiohub.org/public/igem/BBa_K1362130/annotation2410495/range2410495/1
component: BBa_K1362415

https://synbiohub.org/public/igem/BBa_K1362130/annotation2410495/range2410495/1
type: Range
persistent identity: range2410495
display id: range2410495
version: 1
top level: TerDnaE3(N
direction: direction/reverse
start: 1122
end: 1125
orientation: inline

BBa_K1362404
type: SequenceAnnotation
persistent identity: annotation2410496
display id: annotation2410496
version: 1
top level: TerDnaE3(N
location: https://synbiohub.org/public/igem/BBa_K1362130/annotation2410496/range2410496/1
component: BBa_K1362404

https://synbiohub.org/public/igem/BBa_K1362130/annotation2410496/range2410496/1
type: Range
persistent identity: range2410496
display id: range2410496
version: 1
top level: TerDnaE3(N
direction: direction/reverse
start: 1126
end: 1511
orientation: inline

BBa_K1362417
type: SequenceAnnotation
persistent identity: annotation2410499
display id: annotation2410499
version: 1
top level: TerDnaE3(N
location: https://synbiohub.org/public/igem/BBa_K1362130/annotation2410499/range2410499/1
component: BBa_K1362417

https://synbiohub.org/public/igem/BBa_K1362130/annotation2410499/range2410499/1
type: Range
persistent identity: range2410499
display id: range2410499
version: 1
top level: TerDnaE3(N
direction: direction/reverse
start: 1512
end: 1517
orientation: inline

BBa_K1362430
type: SequenceAnnotation
persistent identity: annotation2410502
display id: annotation2410502
version: 1
top level: TerDnaE3(N
location: https://synbiohub.org/public/igem/BBa_K1362130/annotation2410502/range2410502/1
component: BBa_K1362430

https://synbiohub.org/public/igem/BBa_K1362130/annotation2410502/range2410502/1
type: Range
persistent identity: range2410502
display id: range2410502
version: 1
top level: TerDnaE3(N
direction: direction/reverse
start: 1518
end: 1541
orientation: inline

BBa_K1362421
type: SequenceAnnotation
persistent identity: annotation2410504
display id: annotation2410504
version: 1
top level: TerDnaE3(N
location: https://synbiohub.org/public/igem/BBa_K1362130/annotation2410504/range2410504/1
component: BBa_K1362421

https://synbiohub.org/public/igem/BBa_K1362130/annotation2410504/range2410504/1
type: Range
persistent identity: range2410504
display id: range2410504
version: 1
top level: TerDnaE3(N
direction: direction/reverse
start: 1542
end: 1545
orientation: inline

BBa_K1362437
type: SequenceAnnotation
persistent identity: annotation2410506
display id: annotation2410506
version: 1
top level: TerDnaE3(N
location: https://synbiohub.org/public/igem/BBa_K1362130/annotation2410506/range2410506/1
component: BBa_K1362437

https://synbiohub.org/public/igem/BBa_K1362130/annotation2410506/range2410506/1
type: Range
persistent identity: range2410506
display id: range2410506
version: 1
top level: TerDnaE3(N
direction: direction/reverse
start: 1546
end: 1548
orientation: inline

scar
type: ComponentDefinition
persistent identity: BBa G0000
display id: BBa_G0000
version: 1
was generated by: iGEM to SBOL conversion
description: SpeI/XbaI scar for RBS-CDS junctions
created: 2007-07-22T11:00:00Z
modified: 2015-08-31T04:07:27Z
mutable provenance: SpeI/XbaI scar
owned by: james
owned by: myers
mutable description: This is the sequence of the SpeI/XbaI scar for RBS-CDS junctions in BioBricks standard assembly.
discontinued: false
dominant: true
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This is a shorter scar to ensure proper spacing between the RBS and CDS.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_G0000_sequence/1

T7 RBS
type: ComponentDefinition
persistent identity: BBa K1362090
display id: BBa_K1362090
version: 1
was generated by: iGEM to SBOL conversion
description: strong T7 RBS
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:04Z
mutable provenance: synthesized as found in the T7 genome and several commercial expression plasmids.
owned by: james
owned by: myers
mutable description: RFC10 compatible strong RBS derived from the T7 phage gene 10a (major capsid protein)[1]. When assembled to a coding part with the A of the start codon being part of the XbaI site, the RBS will be shifted one bp downstream compared to the native sequence. The sequence was successfully used by the iGEM team Heidelberg 2014 for the expression of many proteins in E.coli. 1. Olinss, P. & Rangwala, S. H. Derived from Bacteriophage T7 mRNA Acts ELS an Enhancer of Translation of the lac2 Gene in. 16973???16976 (1989).
discontinued: false
dominant: false
experience: experience/Works
group u list: _1738_
m user id: 0
owner id: 22830
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: The 18 bp including the XbaI that can be found upstream of the presumably important part of the RBS were included into the sequence just to make sure it works. However to fully comply with RFC10 a G was inserted behind the XbaI site.
star: false
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: t7 RBS
sequence annotation: Shine-Dalgarno
sequence: https://synbiohub.org/public/igem/BBa_K1362090_sequence/1

t7 RBS
type: SequenceAnnotation
persistent identity: annotation2393795
display id: annotation2393795
version: 1
top level: T7 RBS
location: https://synbiohub.org/public/igem/BBa_K1362090/annotation2393795/range2393795/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K1362090/annotation2393795/range2393795/1
type: Range
persistent identity: range2393795
display id: range2393795
version: 1
top level: T7 RBS
direction: direction/forward
start: 11
end: 28
orientation: inline

Shine-Dalgarno
type: SequenceAnnotation
persistent identity: annotation2393796
display id: annotation2393796
version: 1
top level: T7 RBS
location: https://synbiohub.org/public/igem/BBa_K1362090/annotation2393796/range2393796/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K1362090/annotation2393796/range2393796/1
type: Range
persistent identity: range2393796
display id: range2393796
version: 1
top level: T7 RBS
direction: direction/forward
start: 21
end: 28
orientation: inline

G-His6(N)
type: ComponentDefinition
persistent identity: BBa K1362430
display id: BBa_K1362430
version: 1
was generated by: iGEM to SBOL conversion
description: C-terminal His6 (N-Intein)
created: 2014-10-04T11:00:00Z
modified: 2015-05-08T01:10:05Z
mutable provenance: ???
owned by: james
owned by: myers
mutable description: ???
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 22830
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: ???
star: false
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
type: DnaRegion
role: partType/Tag
role: SO:0000324
sequence annotation: His6
sequence annotation: Stop
sequence: https://synbiohub.org/public/igem/BBa_K1362430_sequence/1

His6
type: SequenceAnnotation
persistent identity: annotation2394832
display id: annotation2394832
version: 1
top level: G-His6(N)
location: https://synbiohub.org/public/igem/BBa_K1362430/annotation2394832/range2394832/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_K1362430/annotation2394832/range2394832/1
type: Range
persistent identity: range2394832
display id: range2394832
version: 1
top level: G-His6(N)
direction: direction/forward
start: 4
end: 21
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2394833
display id: annotation2394833
version: 1
top level: G-His6(N)
location: https://synbiohub.org/public/igem/BBa_K1362430/annotation2394833/range2394833/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K1362430/annotation2394833/range2394833/1
type: Range
persistent identity: range2394833
display id: range2394833
version: 1
top level: G-His6(N)
direction: direction/forward
start: 22
end: 24
orientation: inline

RFC105 A
type: ComponentDefinition
persistent identity: BBa K1362414
display id: BBa_K1362414
version: 1
was generated by: iGEM to SBOL conversion
description: N-terminal start overhang (T)(A)-(G)ATG=RBS+Start RFC[105] A
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:05Z
mutable provenance: This standard overhang part was developed as part of the iGEM team Heidelberg 2014's Intein Toolbox [[#References[1]]]. All standard sequences can be reviewed in RFC[???] [[#References[2]]].
owned by: james
owned by: myers
mutable description: This is a standard overhang sequence for in-frame cloning of Proteins of Interest in front or behind an Intein. A detailed cloning strategy is found on the iGEM team Heidelberg 2014's wiki page as well as in RFC[???]. Specifically, this part contains the overhang A used to insert a protein behind any RFC[10] compatible RBS and in-frame with the start-codon. It lies within the four bases formed by the second last base of the XbaI/SpeI scar or the first base in front of start codon respectively and the start codon itself.
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 12377
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This part is only the sequence of a standard overhang. It will not be sent in as physical DNA and was merely created to easily compose new parts in the RFC[???] standard. In the actual cloning process this sequence was and is recommended to be inserted with the according PCR primers.
star: false
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K1362414_sequence/1

RFC105 NN
type: ComponentDefinition
persistent identity: BBa K1362415
display id: BBa_K1362415
version: 1
was generated by: iGEM to SBOL conversion
description: N-terminal splicing overhang TGCT=Cys+1/3 Leu|Ile RFC[105] overhang NN
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:05Z
mutable provenance: This standard overhang part was developed as part of the iGEM team Heidelberg 2014's Intein Toolbox [[#References[1]]]. All standard sequences can be reviewed in RFC[???] [[#References[2]]].
owned by: james
owned by: myers
mutable description: This is a standard overhang sequence for in-frame cloning of Proteins of Interest in front or behind an Intein. A detailed cloning strategy is found on the iGEM team Heidelberg 2014's wiki page as well as in RFC[???]. Specifically, this part contains the overhang A used to insert a protein behind any RFC[10] compatible RBS and in-frame with the start-codon. It lies within the four bases formed by the second last base of the XbaI/SpeI scar or the first base in front of start codon respectively and the start codon itself.
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 12377
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This part is only the sequence of a standard overhang. It will not be sent in as physical DNA and was merely created to easily compose new parts in the RFC[???] standard. In the actual cloning process this sequence was and is recommended to be inserted with the according PCR primers.
star: false
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K1362415_sequence/1

RFC105 NC
type: ComponentDefinition
persistent identity: BBa K1362417
display id: BBa_K1362417
version: 1
was generated by: iGEM to SBOL conversion
description: C-terminal GG-linker overhang GGTGGT=Gly+Gly RFC[105] overhang NC
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:05Z
mutable provenance: This standard overhang part was developed as part of the iGEM team Heidelberg 2014's Intein Toolbox [[#References|[1]]]. All standard sequences can be reviewed in RFC[???] [[#References|[2]]].
owned by: james
owned by: myers
mutable description: This is a standard overhang sequence for in-frame cloning of Proteins of Interest in front or behind an Intein. A detailed cloning strategy is found on the iGEM team Heidelberg 2014's [http://2014.igem.org/Team:Heidelberg/parts wiki page] as well as in RFC[???]. Specifically, this part contains the overhang C used to insert a protein-tag or similar in front of the C-terminal end of a N-Intein. It lies within the a double-glycine linker piece.
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 12377
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This part is only the sequence of a standard overhang. It will not be sent in as physical DNA and was merely created to easily compose new parts in the RFC[???] standard. In the actual cloning process this sequence was and is recommended to be inserted with the according PCR primers.
star: false
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: GlyGly
sequence annotation: RFC[???] C
sequence: https://synbiohub.org/public/igem/BBa_K1362417_sequence/1

GlyGly
type: SequenceAnnotation
persistent identity: annotation2405926
display id: annotation2405926
version: 1
top level: RFC105 NC
location: https://synbiohub.org/public/igem/BBa_K1362417/annotation2405926/range2405926/1
role: feature/scar

https://synbiohub.org/public/igem/BBa_K1362417/annotation2405926/range2405926/1
type: Range
persistent identity: range2405926
display id: range2405926
version: 1
top level: RFC105 NC
direction: direction/forward
start: 1
end: 6
orientation: inline

RFC[???] C
type: SequenceAnnotation
persistent identity: annotation2405925
display id: annotation2405925
version: 1
top level: RFC105 NC
location: https://synbiohub.org/public/igem/BBa_K1362417/annotation2405925/range2405925/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_K1362417/annotation2405925/range2405925/1
type: Range
persistent identity: range2405925
display id: range2405925
version: 1
top level: RFC105 NC
direction: direction/forward
start: 3
end: 6
orientation: inline

RFC105 Z
type: ComponentDefinition
persistent identity: BBa K1362421
display id: BBa_K1362421
version: 1
was generated by: iGEM to SBOL conversion
description: C-terminal stop overhang TAAT=STOP+1/3Stop RFC[105] overhang Z
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:05Z
mutable provenance: This standard overhang part was developed as part of the iGEM team Heidelberg 2014's Intein Toolbox [[#References|[1]]]. All standard sequences can be reviewed in RFC[???] [[#References|[2]]].
owned by: james
owned by: myers
mutable description: This is a standard overhang sequence for in-frame cloning of Proteins of Interest in front or behind an Intein. A detailed cloning strategy is found on the iGEM team Heidelberg 2014's [http://2014.igem.org/Team:Heidelberg/parts wiki page] as well as in RFC[???]. Specifically, this part contains the overhang F used to insert the last part of an intein-fused protein directly in front of an RFC[10] double stop-codon. It lies within the first for bases of the stopstop sequence.
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 12377
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This part is only the sequence of a standard overhang. It will not be sent in as physical DNA and was merely created to easily compose new parts in the RFC[???] standard. In the actual cloning process this sequence was and is recommended to be inserted with the according PCR primers.
star: false
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K1362421_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2402257
display id: annotation2402257
version: 1
top level: RFC105 Z
location: https://synbiohub.org/public/igem/BBa_K1362421/annotation2402257/range2402257/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K1362421/annotation2402257/range2402257/1
type: Range
persistent identity: range2402257
display id: range2402257
version: 1
top level: RFC105 Z
direction: direction/forward
start: 1
end: 4
orientation: inline

mRFP sel.
type: ComponentDefinition
persistent identity: BBa K1362422
display id: BBa_K1362422
version: 1
was generated by: iGEM to SBOL conversion
description: mRFP selection marker with outward BsaI restriction sites
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:05Z
mutable provenance: This selection marker part comes from the iGEM team Heidelberg 2014's Intein Toolbox [[#References|[1]]]. The associated cloning strateges can be reviewed in RFC[???]. The mRFP generator sequence was cloned from <partinfo>BBa_J04450 </partinfo>.
owned by: james
owned by: myers
mutable description: This part is an mRFP selection marker used in the cloning process described in RFC[???] [[#References|[2]]]. It was developed by the iGEM team Heidelberg 2014 as part of our toolbox constructs for intein cloning [[#References|[1]]].
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 12377
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This part will not be sent as physical DNA. It is however a useful part for golden gate cloning.
star: false
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1362424
component: BBa_J04450
component: BBa_K1362423
sequence annotation: BBa_K1362423
sequence annotation: BBa_J04450
sequence annotation: BBa_K1362424
sequence: https://synbiohub.org/public/igem/BBa_K1362422_sequence/1

BBa_K1362424
type: Component
persistent identity: component2410917
display id: component2410917
version: 1
top level: mRFP sel.
definition: BsaI ->
access: public

BBa_J04450
type: Component
persistent identity: component2410916
display id: component2410916
version: 1
top level: mRFP sel.
definition: BBa_J04450
access: public

BBa_K1362423
type: Component
persistent identity: component2410898
display id: component2410898
version: 1
top level: mRFP sel.
definition: <- BsaI
access: public

BBa_K1362423
type: SequenceAnnotation
persistent identity: annotation2410898
display id: annotation2410898
version: 1
top level: mRFP sel.
location: https://synbiohub.org/public/igem/BBa_K1362422/annotation2410898/range2410898/1
component: BBa_K1362423

https://synbiohub.org/public/igem/BBa_K1362422/annotation2410898/range2410898/1
type: Range
persistent identity: range2410898
display id: range2410898
version: 1
top level: mRFP sel.
direction: direction/reverse
start: 1
end: 7
orientation: inline

BBa_J04450
type: SequenceAnnotation
persistent identity: annotation2410916
display id: annotation2410916
version: 1
top level: mRFP sel.
location: https://synbiohub.org/public/igem/BBa_K1362422/annotation2410916/range2410916/1
component: BBa_J04450

https://synbiohub.org/public/igem/BBa_K1362422/annotation2410916/range2410916/1
type: Range
persistent identity: range2410916
display id: range2410916
version: 1
top level: mRFP sel.
direction: direction/reverse
start: 8
end: 1076
orientation: inline

BBa_K1362424
type: SequenceAnnotation
persistent identity: annotation2410917
display id: annotation2410917
version: 1
top level: mRFP sel.
location: https://synbiohub.org/public/igem/BBa_K1362422/annotation2410917/range2410917/1
component: BBa_K1362424

https://synbiohub.org/public/igem/BBa_K1362422/annotation2410917/range2410917/1
type: Range
persistent identity: range2410917
display id: range2410917
version: 1
top level: mRFP sel.
direction: direction/reverse
start: 1077
end: 1083
orientation: inline

TerDnaE3(N
type: ComponentDefinition
persistent identity: BBa K1362404
display id: BBa_K1362404
version: 1
was generated by: iGEM to SBOL conversion
description: TerDnaE3 N-Intein cloning piece
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:05Z
mutable provenance: Part was made using by DNA synthesis. The Sequence is obtained from Liu et al.[[#References|[1]]]
owned by: james
owned by: myers
mutable description: Trichodesmium erythraeum DnaE3-S11 split Intein N-terminal half. This is a DNA piece for cloning used to assemble other BioBrick parts.
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 12377
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This part represents only the intein sequence without inluding the standard splicing-site or polyglycine-linker overhangs.
star: false
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
sequence: https://synbiohub.org/public/igem/BBa_K1362404_sequence/1

Stop
type: ComponentDefinition
persistent identity: BBa K1362437
display id: BBa_K1362437
version: 1
was generated by: iGEM to SBOL conversion
description: Tripple A
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:05Z
owned by: james
owned by: myers
mutable description: Triple A yielding an second stop codon if behind RFC[???] overhang F
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 22911
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K1362437_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2402256
display id: annotation2402256
version: 1
top level: Stop
location: https://synbiohub.org/public/igem/BBa_K1362437/annotation2402256/range2402256/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K1362437/annotation2402256/range2402256/1
type: Range
persistent identity: range2402256
display id: range2402256
version: 1
top level: Stop
direction: direction/forward
start: 1
end: 2
orientation: inline

BBa_J04450
type: ComponentDefinition
persistent identity: BBa J04450
display id: BBa_J04450
version: 1
was generated by: iGEM to SBOL conversion
description: RFP Coding Device
created: 2005-06-08T11:00:00Z
modified: 2015-08-31T04:08:14Z
mutable provenance: Davidson Synth-Aces
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Contains an IPTG inducible promoter an RBS, RFP, and terminator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _16_
m user id: 0
owner id: 328
owning group id: 16
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Tamar Odle
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_B0034
component: BBa_R0010
component: BBa_B0012
component: BBa_B0010
component: BBa_E1010
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J04450_sequence/1

BBa_B0034
type: Component
persistent identity: component1509404
display id: component1509404
version: 1
top level: BBa_J04450
definition: BBa_B0034
access: public

BBa_R0010
type: Component
persistent identity: component1509394
display id: component1509394
version: 1
top level: BBa_J04450
definition: LacI
access: public

BBa_B0012
type: Component
persistent identity: component1509427
display id: component1509427
version: 1
top level: BBa_J04450
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1509417
display id: component1509417
version: 1
top level: BBa_J04450
definition: BBa_B0010
access: public

BBa_E1010
type: Component
persistent identity: component1509411
display id: component1509411
version: 1
top level: BBa_J04450
definition: mRFP1
access: public

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1509394
display id: annotation1509394
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509394/range1509394/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_J04450/annotation1509394/range1509394/1
type: Range
persistent identity: range1509394
display id: range1509394
version: 1
top level: BBa_J04450
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1509404
display id: annotation1509404
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509404/range1509404/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J04450/annotation1509404/range1509404/1
type: Range
persistent identity: range1509404
display id: range1509404
version: 1
top level: BBa_J04450
direction: direction/forward
start: 209
end: 220
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1509411
display id: annotation1509411
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509411/range1509411/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_J04450/annotation1509411/range1509411/1
type: Range
persistent identity: range1509411
display id: range1509411
version: 1
top level: BBa_J04450
direction: direction/forward
start: 227
end: 907
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1509417
display id: annotation1509417
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509417/range1509417/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J04450/annotation1509417/range1509417/1
type: Range
persistent identity: range1509417
display id: range1509417
version: 1
top level: BBa_J04450
direction: direction/forward
start: 941
end: 1020
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1509427
display id: annotation1509427
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509427/range1509427/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J04450/annotation1509427/range1509427/1
type: Range
persistent identity: range1509427
display id: range1509427
version: 1
top level: BBa_J04450
direction: direction/forward
start: 1029
end: 1069
orientation: inline

BsaI ->
type: ComponentDefinition
persistent identity: BBa K1362424
display id: BBa_K1362424
version: 1
was generated by: iGEM to SBOL conversion
description: BsaI restriction site for RFC[105] cloning
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:05Z
mutable provenance: This standard restriction site sequence is used as part of the iGEM team Heidelberg 2014's Intein Toolbox [[#References|[1]]]. All associated standard sequences can be reviewed in RFC[???]
owned by: james
owned by: myers
mutable description: This is the reverse complement of a BsaI restriction site headed by an Adenine as a spacer-base to separate the recognition sequence from the outward-lying cutting sequence. It was used by us for scarless golden-gate cloning to fuse inteins to other proteins and thereby implement a variety of possible port-translational modifications.
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 12377
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This part is only the sequence of a restriction site. It will not be sent in as physical DNA and was merely created to easily compose new parts in the RFC[???] standard. In the actual cloning process this sequence was and is recommended to be inserted with the according PCR primers.
star: false
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K1362424_sequence/1

<- BsaI
type: ComponentDefinition
persistent identity: BBa K1362423
display id: BBa_K1362423
version: 1
was generated by: iGEM to SBOL conversion
description: BsaI reverse restriction site for RFC[105] cloning
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:05Z
mutable provenance: This standard restriction site sequence is used as part of the iGEM team Heidelberg 2014's Intein Toolbox [[#References|[1]]]. All associated standard sequences can be reviewed in RFC[???]
owned by: james
owned by: myers
mutable description: This is the reverse complement of a BsaI restriction site headed by an Adenine as a spacer-base to separate the recognition sequence from the outward-lying cutting sequence. It was used by us for scarless golden-gate cloning to fuse inteins to other proteins and thereby implement a variety of possible port-translational modifications.
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 12377
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This part is only the sequence of a restriction site. It will not be sent in as physical DNA and was merely created to easily compose new parts in the RFC[???] standard. In the actual cloning process this sequence was and is recommended to be inserted with the according PCR primers.
star: false
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K1362423_sequence/1

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

https://synbiohub.org/public/igem/BBa_K1362130_sequence/1
type: Sequence
persistent identity: BBa K1362130 sequence
display id: BBa_K1362130_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aataattttgtttaactttaagaaggagatactagatgagagacccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttataggtctcatgcttaacttacgaaactgagatcatgaccgttgagtatggtccgctgccgatcggcaaaattgttgaatatcgcatcgagtgcaccgtgtacaccgtggacaaaaacggctacatctacactcagccgattgcgcagtggcacaaccgcggtatgcaggaagtatatgaatattccctggaagatggcactgttatccgcgctaccccagagcacaaattcatgactgaggatggtcagatgctgccgatcgatgaaatcttcgaacgtaacctggacctgaaatgtctgggtacgctggaagtaaaaattgttagccgtaaactggcaaaaaccgaaaacgtatatgatatcggcgttaccaaggaccataacttcgttctggcaaacggtggtggtcatcatcaccatcaccattagtaataaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G0000_sequence/1
type: Sequence
persistent identity: BBa G0000 sequence
display id: BBa_G0000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1362090_sequence/1
type: Sequence
persistent identity: BBa K1362090 sequence
display id: BBa_K1362090_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aataattttgtttaactttaagaaggaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1362430_sequence/1
type: Sequence
persistent identity: BBa K1362430 sequence
display id: BBa_K1362430_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggtcatcatcaccatcaccattag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1362414_sequence/1
type: Sequence
persistent identity: BBa K1362414 sequence
display id: BBa_K1362414_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1362415_sequence/1
type: Sequence
persistent identity: BBa K1362415 sequence
display id: BBa_K1362415_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1362417_sequence/1
type: Sequence
persistent identity: BBa K1362417 sequence
display id: BBa_K1362417_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggtggt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1362421_sequence/1
type: Sequence
persistent identity: BBa K1362421 sequence
display id: BBa_K1362421_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1362422_sequence/1
type: Sequence
persistent identity: BBa K1362422 sequence
display id: BBa_K1362422_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agagacccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttataggtctca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1362437_sequence/1
type: Sequence
persistent identity: BBa K1362437 sequence
display id: BBa_K1362437_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1362404_sequence/1
type: Sequence
persistent identity: BBa K1362404 sequence
display id: BBa_K1362404_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taacttacgaaactgagatcatgaccgttgagtatggtccgctgccgatcggcaaaattgttgaatatcgcatcgagtgcaccgtgtacaccgtggacaaaaacggctacatctacactcagccgattgcgcagtggcacaaccgcggtatgcaggaagtatatgaatattccctggaagatggcactgttatccgcgctaccccagagcacaaattcatgactgaggatggtcagatgctgccgatcgatgaaatcttcgaacgtaacctggacctgaaatgtctgggtacgctggaagtaaaaattgttagccgtaaactggcaaaaaccgaaaacgtatatgatatcggcgttaccaaggaccataacttcgttctggcaaac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1362424_sequence/1
type: Sequence
persistent identity: BBa K1362424 sequence
display id: BBa_K1362424_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggtctca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J04450_sequence/1
type: Sequence
persistent identity: BBa J04450 sequence
display id: BBa_J04450_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1362423_sequence/1
type: Sequence
persistent identity: BBa K1362423 sequence
display id: BBa_K1362423_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agagacc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z