LOCUS       BBa_K1362141            1583 bp    DNA     linear       25-Nov-2024
DEFINITION  RBS + SspDnaB C-intein RFC[105] assembly construct (with His6)
ACCESSION   BBa_K1362141
VERSION     BBa_K1362141.1  
FEATURES             Location/Qualifiers
     RBS             11..28
                     /label=t7 RBS
     misc_feature    21..28
                     /label=Shine-Dalgarno
     RBS             1..29
                     /label=BBa_K1362090
     misc_feature    30..35
                     /label=BBa_G0000
     misc_feature    36..38
                     /label=BBa_K1362414
     misc_feature    39..56
                     /label=His6
     misc_feature    57..59
                     /label=Gly
     misc_feature    39..59
                     /label=BBa_K1362431
     misc_feature    60..63
                     /label=BBa_K1362418
     misc_feature    64..65
                     /label=BBa_K1362467
     misc_feature    66..490
                     /label=BBa_K1362409
     misc_feature    491..494
                     /label=BBa_K1362419
     misc_feature    495..501
                     /label=BBa_K1362423
     CDS             502..589
                     /label=end of LacI coding region (inactive)
     misc_feature    502..701
                     /label=BBa_R0010
     misc_feature    590..627
                     /label=CAP binding site
     promoter        638..643
                     /label=-35
     promoter        662..667
                     /label=-10
     misc_feature    667..701
                     /label=LacI binding site
     misc_feature    674..674
                     /label=start
     promoter        502..701
                     /label=BBa_R0010
     misc_feature    714..717
                     /label=conserved
     RBS             710..721
                     /label=BBa_B0034
     CDS             728..1402
                     /label=mrfp1
     misc_feature    1409..1433
                     /label=Help:Barcodes
     CDS             728..1408
                     /label=BBa_E1010
     stem_loop       1453..1496
                     /label=stem_loop
     misc_feature    1442..1521
                     /label=BBa_B0010
     terminator      1442..1521
                     /label=BBa_B0010
     stem_loop       1537..1556
                     /label=T7 TE
     misc_feature    1563..1563
                     /label=stop
     polyA_site      1557..1570
                     /label=polya
     misc_feature    1530..1570
                     /label=BBa_B0012
     terminator      1530..1570
                     /label=BBa_B0012
     misc_feature    502..1570
                     /label=BBa_J04450
     misc_feature    1571..1577
                     /label=BBa_K1362424
     misc_feature    495..1577
                     /label=BBa_K1362422
     misc_feature    1578..1581
                     /label=stop
     misc_feature    1578..1581
                     /label=BBa_K1362421
     misc_feature    1582..1583
                     /label=BBa_K1362465
ORIGIN
        1 aataattttg tttaacttta agaaggagat actagatgca tcatcaccat caccacggtg
       61 gtggtagcac aggaaaaaga gtttctatta aagatttgtt agatgaaaaa gattttgaaa
      121 tatgggcaat taatgaacag acgatgaagc tagaatcagc taaagttagt cgtgtatttt
      181 gtactggcaa aaagctagtt tatattctaa aaactcgact aggtagaact atcaaggcaa
      241 cagcaaatca tagattttta actattgatg gttggaaaag attagatgag ctatctttaa
      301 aagagcatat tgctctaccc cgtaaactag aaagctcctc tttacaattg tcaccagaaa
      361 tagaaaagtt gtctcagagt gatatttact gggactccat cgtttctatt acggagactg
      421 gagtcgaaga ggtttttgat ttgactgtgc caggaccaca taactttgtc gccaatgaca
      481 tcattgtcca caacagagac ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
      541 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
      601 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
      661 gtatgttgtg tggaattgtg agcggataac aatttcacac atactagaga aagaggagaa
      721 atactagatg gcttcctccg aagacgttat caaagagttc atgcgtttca aagttcgtat
      781 ggaaggttcc gttaacggtc acgagttcga aatcgaaggt gaaggtgaag gtcgtccgta
      841 cgaaggtacc cagaccgcta aactgaaagt taccaaaggt ggtccgctgc cgttcgcttg
      901 ggacatcctg tccccgcagt tccagtacgg ttccaaagct tacgttaaac acccggctga
      961 catcccggac tacctgaaac tgtccttccc ggaaggtttc aaatgggaac gtgttatgaa
     1021 cttcgaagac ggtggtgttg ttaccgttac ccaggactcc tccctgcaag acggtgagtt
     1081 catctacaaa gttaaactgc gtggtaccaa cttcccgtcc gacggtccgg ttatgcagaa
     1141 aaaaaccatg ggttgggaag cttccaccga acgtatgtac ccggaagacg gtgctctgaa
     1201 aggtgaaatc aaaatgcgtc tgaaactgaa agacggtggt cactacgacg ctgaagttaa
     1261 aaccacctac atggctaaaa aaccggttca gctgccgggt gcttacaaaa ccgacatcaa
     1321 actggacatc acctcccaca acgaagacta caccatcgtt gaacagtacg aacgtgctga
     1381 aggtcgtcac tccaccggtg cttaataacg ctgatagtgc tagtgtagat cgctactaga
     1441 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     1501 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
     1561 tgcgtttata ggtctcataa taa
//